Difference between revisions of "Team:WashU StLouis/Parts"
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| − | <p style="font-size:4vw"> Our Parts </p> | + | <p style="font-size:4vw; text-align:center"> Our Parts </p> |
<p>Our team designed several parts that are intended to confer resistance to UV-B radiation in E. coli. Our basic parts encode radiation-resistance proteins, while our composite parts combine these proteins with an inducible Lac reporter and blue chromoprotein, a reporter.</p> | <p>Our team designed several parts that are intended to confer resistance to UV-B radiation in E. coli. Our basic parts encode radiation-resistance proteins, while our composite parts combine these proteins with an inducible Lac reporter and blue chromoprotein, a reporter.</p> | ||
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| − | <p style="font-size:2vw"> Basic Parts </p> | + | <p style="font-size:2vw; text-align:center"> Basic Parts </p> |
<p> These basic parts are all coding sequences that came from foreign organisms, and we hoped would confer radiation resistance to E. Coli and our Cyanobacteria strain</p> | <p> These basic parts are all coding sequences that came from foreign organisms, and we hoped would confer radiation resistance to E. Coli and our Cyanobacteria strain</p> | ||
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| − | <p style="font-size:2vw"> Composite Parts </p> | + | <p style="font-size:2vw; text-align:center"> Composite Parts </p> |
<p> Two of our composite parts contain Lac-inducible forms of our resistance genes. One is a reporter using the Blue Chromoprotein that we used as a control. The last is an antibiotic resistance gene that we improved by optimizing for <i>E. Coli</i></p> | <p> Two of our composite parts contain Lac-inducible forms of our resistance genes. One is a reporter using the Blue Chromoprotein that we used as a control. The last is an antibiotic resistance gene that we improved by optimizing for <i>E. Coli</i></p> | ||
Revision as of 01:49, 2 November 2017
Our Parts
Our team designed several parts that are intended to confer resistance to UV-B radiation in E. coli. Our basic parts encode radiation-resistance proteins, while our composite parts combine these proteins with an inducible Lac reporter and blue chromoprotein, a reporter.
Basic Parts
These basic parts are all coding sequences that came from foreign organisms, and we hoped would confer radiation resistance to E. Coli and our Cyanobacteria strain
| Part Name | Part Number | Part Function |
|---|---|---|
| Dsup | BBa_K2195000 | Tardigrade DNA binding protein, provides radiation resistance |
| phrAc | BBa_K2195001 | Cyanobacteria DNA photolyase, provides radiation resistance |
| phrAt | BBa_K2195006 | Tardigrade DNA photolyase, provides radiation resistance |
Composite Parts
Two of our composite parts contain Lac-inducible forms of our resistance genes. One is a reporter using the Blue Chromoprotein that we used as a control. The last is an antibiotic resistance gene that we improved by optimizing for E. Coli
| Part Name | Part Number | Part Function |
|---|---|---|
| uvsE Full Gene | BBa_K2195008 | Synthesized gene of Lac Promoter, RBS and uvsE |
| Dsup Full Gene | BBa_K2195005 | Synthesized gene of Lac Promoter, RBS and Dsup |
| Blue Chromoprotein Reporter | BBa_K2195007 | Synthesized gene of Lac Promoter, RBS and BCP |
| Neomycin Phosphotransferase full gene | BBa_K2195002 | Confers antibiotic resistance to Kanamycin and Neomycin |
