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...ted BBa_K1031803 colonies grew. Took 2 colonies, sampled for colony PCR. Frozen stock. Liquid culture in CM - Took colonies, frozen stock, colony PCR, liquid culture. Called it PK.XR

...ano drop mini prep PrD-CFP PXP218, PrD-cYFP PSB1C3, and PrD PSB1C3', 'Make frozen stock of PrD-YFP PSB1C3 and PrD-CFP PSB1C3', 'Miniprep pXP216 and pXP420 v2

<br/>- Resuspend (frozen) cell pellet in cracking buffer (100 µl of cracking buffer per 7,5 total O  Frozen Competent Cell (FCC) Solution → Solution is 5% (v/v) glycerol and 10% (v/

repeatedly frozen and thawed, it starts to ‘die’ after even one The competent cells can also be used fresh, without making frozen aliquots,

...nvolving calcium chloride. As the final step in preparation all cells were frozen in either 50 &micro;l or 100 &micro;l volumes by submersion in liquid nitro .... Purified DNA concentration was checked using a nanodrop and samples were frozen in a -20&#176;C freezer for further use.</p>

repeatedly frozen and thawed, it starts to ‘die’ after even one The competent cells can also be used fresh, without making frozen aliquots,

<li>The glycerol stock should then be frozen at -80 o C<ul> <li>The glycerol stock should then be frozen at -80 o C<ul>

<li>The cells were frozen at -80ºC. </li> <li>Top10 chemically competent cells (100 µl frozen stock) </li>

<li>Streak cells from frozen stock onto LB plate. Incubate O/N at 37&deg;C.</li> ...microcentrifuge tubes (on ice). Freeze immediately on dry ice. Stire cells frozen at -80&deg;C.</i></li>

<p>Once the wikis are frozen, users can no longer make any changes until they are unfrozen after the Jam ...iant Jamboree. Since judges start reviewing iGEM teams once the Wiki's are frozen, this is a very real deadline and no edits will be allowed after the deadli

bonded over frozen custard and ice cream.</p> ...n a test tube. The team took pictures of each sample. The tubes were then frozen in a -80༠ freezer.

<li>Once the cultures are frozen, transfer them to a -80°C freezer. The cultures should be good for 3 weeks <li>Thaw frozen tube of competent cells on ice.</li>

...thawed, so we took a pipette tip and scraped some of the top layer of the frozen tube and plated those. Plate was a standard ampicillin plate.</p> ...th the completion of our Abstract and finalised title. Welcome to project "Frozen in Thyme" </p>

<h3>Frozen</h3> <h3>Frozen</h3>

.... Pellet was resuspended in Buffer A (50 mM NaPi, 300 mM NaCl, pH 8.0) and frozen in liquid nitrogen before storing at -20 °C. Frozen cells were thawed in hand-warm water bath before lysing them with EmulsiFle

and can spoil if frozen (<a href="http://www.who.int/immunization/documents/IIP2015_Module2.pdf" ta simple tests for whether or not the vaccines had frozen beyond the point of safety,

<li>Pour off supernatant, sample can be used immediately or frozen at -20C.</li> <li>Pour off supernatant, sample can be used immediately or frozen at -20C.</li>

<p><ul class="ulclass"><li> • Frozen chemically competent cells (100 µL per tube) are thawed on ice. ...rile AD with 10 % glycerol. Cells are ready for transformation or could be frozen at -80°C until transformation.

...n liquid nitrogen at -200°C and placed on dry ice. After all samples were frozen, the tube lids were pierced and placed in a chilled vacuum chamber overnigh

<li>Thaw competent cells rapidly by immersing frozen tubes in a 37°C water bath. </li> ...0 ml) with 4 ml EGTA (0.1 M, pH 8.0) but without CaCl2. SpII + EGTA can be frozen at -20°C in small aliquots, although repeated freeze-thawing should be avo