Team:Bilkent-UNAMBG/Parts

Parts

Parts

Name

Type

Description

Length (bp)

BBa_K2234000

Regulatory

MimR-acetone responsive transcription factor: activates transcription from pMimA promoter when acetone is present.

1782

BBa_K2234001

Regulatory

pMimA: MimR (BBa_K2234000) activates pMimA with respect to acetone.

247

BBa_K2234002

Coding

LuxA CDS from V.fischeri : encodes for LuxA from Vibrio fischeri, a luminescent bacteria found in ocean.

1065

BBa_K2234003

Coding

LuxB CDS from V.fischeri: encodes for LuxB from Vibrio fischeri, a luminescent bacteria found in ocean.

981

BBa_K2234004

Coding

LuxG CDS from V.fischeri: encodes for LuxG from Vibrio fischeri, a luminescent bacteria found in ocean.

711

BBa_K2234005

Device

HxlR- pFor based formaldhyde sensor: HxlR is a regulatory protein from Bacillus subtilis that controls a formaldehyde sensitive operon. A sensor was constructed using HxlR, coupled with its respective promoter and a reporter gene.

1307

BBa_K2234006

Generator

mRFP1 generator: This part produces mRFP1 constitutively.

767

BBa_K2234007

Generator

tannenRFP generator: This part produces TannenRFP constitutively.

739

BBa_K2234008

Generator

amilGFP generator: This part produces AmilGFP constitutively.

760

BBa_K2234009

Generator

tsPurple generator: This part produces tsPurple constitutively.

888

BBa_K2234010

Generator

amilCP generator: This part produces amilCP constitutively.

867

BBa_K2234011

Regulatory

AlkR-pAlkB from Acinetobacter baylyi: AlkR is the regulatory protein of Alk operon in Acinetobacter baylyi. This operon is responsible for mid-long chain alkane degredation. It activates the promoter of AlkB gene, named pAlkB.

1274

BBa_K2234012

Regulatory

HxlR- pFor from Bacillus Subtilis: HxlR is a regulatory protein from Bacillus subtilis that controls a formaldehyde sensitive operon. This part includes HxlR CDS and the promoter it regulates.

632

BBa_K2234022

Device

Cyclohexanone Sensor: produces with respect to cyclohexanone.

2298

BBa_K2234023

Composite

pChnB-sfGFP: produces sfGFP with respect to cyclohexanone.

1152

BBa_K2234024

Device

AlkR-pAlkB based long-mid chain alkane sensor: AlkR is the regulatory protein of Alk operon in Acinetobacter baylyi. This operon is responsible for mid-long chain alkane degredation. It activates the promoter of AlkB gene, named pAlkB. This part consists of AlkR CDS under a ATC inducible promoter and sfGFP under a AlkR regulated promoter.

2058

BBa_K2234025

Device

HxlR-pFor based formaldehyde sensor 1.1: HxlR is a regulatory protein from Bacillus subtilis that controls a formaldehyde sensitive operon. This part consists of HxlR under a ATC inducible promoter and sfGFP under a HxlR regulated promoter.

1431

BBa_K2234026

Device

MimR-pmimA based acetone sensor: MimR is under the control of a ATC inducible promoter. MimR activates transcription from pMimA promoter when acetone is present. Activated promoter results in sfGFP production.

2894

BBa_S05419

Intermediate

B0032-K1321337: construction intermediate

732

BBa_S05420

Intermediate

S05419-B0015: construction intermediate

869

BBa_S05421

Intermediate

K2234021-B0015: construction intermediate

1095

BBa_S05422

Intermediate

J23106-S05421: construction intermediate

1138