Team:Evry Paris-Saclay/Basic Part
Basic Parts
During this project, our team designed the following basic BioBricks:
Coding sequences:
| Part number | Name | Submitted to the registry | Improves the function of an existing BioBrick Part |
|---|---|---|---|
| BBa_K2448001 | mEmerald | yes | |
| BBa_K2448003 | LacZ-alpha (pUC19 like) | ||
| BBa_K2448004 | mCherry | yes | Yes of Part:BBa_J06504 |
| BBa_K2448006 | PsiR from Agrobacterium tumefaciens | ||
| BBa_K2448007 | PsiR from Sinorhizobium fredii | ||
| BBa_K2448008 | PsiR from Sinorhizobium meliloti | ||
| BBa_K2448009 | FruR from Escherichia coli | ||
| BBa_K2448021 | D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum | ||
| BBa_K2448022 | D-Psicose 3-epimerase (Dpe) from Agrobacterium tumefaciens |
Plasmid backbones:
| Part number | Name | Submitted to the registry | Improves the function of an existing BioBrick Part |
|---|---|---|---|
| BBa_K2448036 | pSB1C3 BsmBI free | yes | Yes of Part:pSB1C3 |
| BBa_K2448037 | pSB3K3 BsaI free | yes | Yes of Part:pSB3K3 |
| BBa_K2448038 | pSB1C3 LacIq | yes | Yes of Part:pSB1C3 |
Ribosomal binding sites (RBS):
| Part number | Name | Submitted to the registry | Improves the function of an existing BioBrick Part |
|---|---|---|---|
| BBa_K2448000 | B0034 with scars AGGA and AGATCA | ||
| BBa_K2448002 | B0034 like RBS with scars AATT and CAGCT | ||
| BBa_K2448020 | B0034 with scars TAC and CAT | ||
| BBa_K2448039 | B0034 with scars GTAC and TACCAT | ||
| BBa_K2448049 | B0034 with scars GTAC and CTCGAGg | ||
| BBa_K2448019 | RBS (pET like) |
Promoters:
| Part number | Name | Submitted to the registry | Improves the function of an existing BioBrick Part |
|---|---|---|---|
| BBa_K2448016 | pPsiTac1 | Yes of Part:BBa_K864400 | |
| BBa_K2448050 | pPsiTac2 | Yes of Part:BBa_K864400 | |
| BBa_K2448010 | pPsiA from Agrobacterium tumefaciens | ||
| BBa_K2448011 | pPsiR from Agrobacterium tumefaciens | ||
| BBa_K2448012 | pPsiA from Sinorhizobium fredii | ||
| BBa_K2448013 | pPsiR from Sinorhizobium fredii | ||
| BBa_K2448014 | pPsiA from Sinorhizobium meliloti | ||
| BBa_K2448015 | pPsiR from Sinorhizobium meliloti | ||
| BBa_K2448017 | pFruB from Escherichia coli |
Terminators:
| Part number | Name | Submitted to the registry | Improves the function of an existing BioBrick Part |
|---|---|---|---|
| BBa_K2448005 | B0015 with scars T and TGTT | ||
| BBa_K2448051 | B0015 with scars AGCGGCATGC and GCAGA | ||
| BBa_K2448018 | L3S1P51 terminator | ||
| BBa_K2448052 | L2U3H03 Terminator |
And various spacers in between the functional elements of our composite parts containing cloning sites :
| Part number | Name | Submitted to the registry | Improves the function of an existing BioBrick Part |
|---|---|---|---|
| BBa_K2448040 | spacer with cloning sites XhoI and BsmBI | ||
| BBa_K2448041 | spacer with cloning sites BsmBI and SphI | ||
| BBa_K2448042 | spacer with cloning sites BamHI and BbsI | ||
| BBa_K2448043 | spacer with cloning sites BbsI and NcoI | ||
| BBa_K2448044 | spacer with cloning sites XhoI, BsmBI x2, SphI | ||
| BBa_K2448045 | spacer with cloning sites BamHI, BbsI x2, NcoI | ||
| BBa_K2448046 | spacer with cloning site SphI | ||
| BBa_K2448047 | spacer with cloning site BamHI | ||
| BBa_K2448048 | spacer with cloning site NcoI |
