CALENDAR
AUGUST
SUN | MON | TUE | WED | THU | FRI | SAT |
---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | ||
6 interlab | 7 interlab | 8 | 9 | 10 | 11 | 12 |
13 | 14 | 15 | 16 | 17 S.C genomic DNA extraction | 18 Transformation(A-ko) | 19 Transformation(A-ko) |
20 Colony PCR(A-ko) | 21 PCR(pSB1C3) Transformation(A-ko) | 22 Colony PCR(A-ko) | 23 PCR(A-ko) Colony PCR(A-ko) | 24 Colony PCR(A-ko) PCR(R from S.C genome) | 25 PCR(R,P from S.C genome) Transformation(A-ko) | 26 PCR(R,P) Colony PCR(A-ko) |
27 | 28 | 29 | 30 | 31 PCR(R,P) |
SEPTEMBER
SUN | MON | TUE | WED | THU | FRI | SAT |
---|---|---|---|---|---|---|
1 PCR(H-an,A-ko) | 2 | |||||
3 | 4 PCR(R,P) Transformation(A-ko,H-an,R,P) | 5 Colony PCR(A-ko,H-an,R,P) PCR(R,P) | 6 PCR(A-ko,H-an,R,P) Transformation(A-ko,H-an,R,P) | 7 Colony PCR(H-an,R) PCR(A-ko,H-an,R,P) Transformation(A-ko,H-an,P) | 8 Colony PCR(A-ko,P) PCR(R,P) | 9 |
10 Colony PCR(A-ko,H-an,P) | 11 Transformation(A-ko+R,A-ko+P,H-an+R,H-an+P) | 12 Colony PCR(A-ko+R,A-ko+P,H-an+R,H-an+P) | 13 PCR(A-ko,H-an) | 14 PCR(A-ko,H-an,pSB1C3) Transformation(A-ko,H-an) | 15 Colony PCR(A-ko,H-an) Transformation(A-ko,H-an) | 16 Transformation(A-ko+R,A-ko+P,H-an+R,H-an+P) |
17 Colony PCR(A-ko+R,A-ko+P,H-an+R,H-an+P) | 18 | 19 | 20 | 21 interlab Transformation(A-ko+R,A-ko+P,H-an+R,H-an+P) | 22 Colony PCR(A-ko+R,A-ko+P,H-an+R,H-an+P) | 23 |
24 | 25 Transformation(A-ko+R,A-ko+P,H-an+R,H-an+P) | 26 Colony PCR(A-ko+R,A-ko+P,H-an+R,H-an+P) | 27 Transformation(A-ko+R,A-ko+P,H-an+R,H-an+P) Colony PCR(A-ko+R,A-ko+P,H-an+R,H-an+P) | 28 Colony PCR(A-ko+R,A-ko+P,H-an+R,H-an+P) | 29 Colony PCR(A-ko+R,A-ko+P,H-an+R,H-an+P) | 30 Transformation(A-ko+R,A-ko+P,H-an+R,H-an+P) |
OCTOBER
SUN | MON | TUE | WED | THU | FRI | SAT |
---|---|---|---|---|---|---|
1 Colony PCR(A-ko+R,A-ko+P,H-an+R,H-an+P) | 2 Transformation(A-ko+P,H-an+P) | 3 | 4 Colony PCR(A-ko+P,H-an+P) Transformation(A-ko+R,A-ko+P,H-an+P) | 5 | 6 Transformation(A-ko+R,A-ko+P,H-an+P) | 7 Colony PCR(A-ko+R,A-ko+P,H-an+P) Transformation(R,P) |
8 Colony PCR(R,P) | 9 | 10 | 11 | 12 PCR(H-an+R) | 13 | 14 Transformation(A-ko+R,A-ko+P,H-an+P) |
15 Colony PCR(A-ko+R,A-ko+P,H-an+P) PCR(R,P) | 16 | 17 Transformation(A-ko+R,A-ko+P,H-an+P) | 18 Colony PCR(A-ko+R,A-ko+P,H-an+P) | 19 PCR(H) | 20 PCR(H) Transformation(A-ko+P,H-an+P,H) | 21 Colony PCR(H) Transformation of yeast(H-an+R) |
22 Transformation(A-ko+P,H-an+P) | 23 Colony PCR(A-ko+P,H-an+P) | 24 Colony PCR of yeast(H-an+R) Transformation of yeast | 25 | 26 PCR(pESC-LEU) | 27 PCR(pESC-LEU) | 28 PCR(pESC-LEU) Colony PCR of yeast |
29 Colony PCR of yeast | 30 | 31 |
S.C = Saccharomyces cerevisiae
A-ko = ADH1-Kozak-GFP
H-an = HXT3-antisenseGFP
R = RPL15A
P = PGK1
H = HXT3