Team:SDSZ-China/Parts
Parts
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K2239001 | Generator | ChBD-7alphaHSDH | Ruohong Wang | 1089 |
| BBa_K2239002 | Generator | ChBD-7betaHSDH | Ruohong Wang | 1179 |
| BBa_K2239003 | Generator | ChBD-LDH | Ruohong Wang | 1323 |
| BBa_K2239004 | Generator | ChBD-GDH | Ruohong Wang | 1107 |
| BBa_K2239005 | Generator | ChBD-GFP | Ruohong Wang | 1047 |
| BBa_K2239006 | Generator | CBD-7alphaHSDH | Ruohong Wang | 1080 |
| BBa_K2239007 | Generator | CBD-7betaHSDH | Ruohong Wang | 1170 |
| BBa_K2239008 | Generator | CBD-LDH | Ruohong Wang | 1314 |
| BBa_K2239009 | Generator | CBD-GDH | Ruohong Wang | 1098 |
| BBa_K2239010 | Generator | CBD-GFP | Ruohong Wang | 1038 |
| BBa_K2239011 | Coding | 7alpha-HSDH | Ruohong Wang | 780 |
| BBa_K2239012 | Coding | 7beta-HSDH | Ruohong Wang | 870 |
| BBa_K2239013 | Coding | GFP | Ruohong Wang | 738 |
| BBa_K2239014 | Coding | GDH (glucose dehydrogenas) | Ruohong Wang | 798 |
| BBa_K2239015 | Coding | LDH ( lactate dehydrogenase) | Ruohong Wang | 1014 |
Contribution
We improved the description of CBD and ChBD on previous part pages.
Proof of the CBD function
The resulted solution after expression is mixed with a gauze piece, and the green color on gauze is recorded. Then the gauze is washed three times using ddH2O, but the green fluorescent on it is not significantly reduced, proving that the CDB is well functioned. In comparison, no green fluorescent is left after washing the gauze mixed with GFP-ChBD (Chintin binding domain).
[Fig. 1. GFP-CBD on gauze before washing]
[Fig. 2. GFP-CBD on gauze after washing]
[Fig. 3. GFP-ChBD on gauze before washing]
[Fig. 4. GFP-ChBD on gauze after washing]
Proof of the ChBD function
The resulted solution after expression is mixed with chitin powder. Also, the solution with GFP-ChBD but without chitin, solution with the presence of GFP and chitin, and water in comparison, are presented.
Tube 3 contains GFP-ChBD, with a significant green color in the solution. After adding chitin powder inside the tube, the color in the solution disappears and shows on the chitin powder, as presented in tube 2. It proves that the ChBD successfully binds the GFP onto the chitin powder. In contrast, the color of the solution is not changed when adding chitin powder into the GFP solution without ChBD. As a result, the ChBD is well functioned.
[Fig.5 ChBD function: tube1, GFP and chitin; tube2, GFP-ChBD and chitin; tube3, GFP-ChBD; tube4, water]
