Team:Stony Brook/Notebook/Week11
Week 11
8/06:
• Prepared samples of GST A53 and GST LnqZ to run SDS-PAGE
• Ran SDS-PAGE
• Viewed SDS-PAGE→ faint protein bands in elution 1 for both A53 and LnqZ, but not in any other elutions
8/07:
• Nanodropped our elutions of MBP protein purification products
• Nanodropped our elutions of GST protein purification products
• Freeze-Thaw of SUMO LnqZ and Epi
• Made liquid cultures of
• MBP lnqZE
• MBP lnqZA
• MBP lnqZQ
8/08:
• Nanodrop elutions of SUMO Epi
• Nanodrop elutions of SUMO Lnq Z
8/09:
• Continued expression and purification of our proteins.
8/10:
• Continued expression and purification of our proteins.
• Discussed improvements on our purification protocol with our advisor Dr. Steven Glynn.
8/11:
• Continued expression and purification of our proteins.
• Applied new improvements to our purification protocol, such as the use of a sonicator
