Week 12
8/14:
• Continued expression and purification of our proteins.
8/15:
• Nanodrop of MBP lnqZE Elutions
• Nanodrop of MBP lnqZA Elutions
• Nanodrop of MBP lnqZQ Elutions
8/16:
• Nanodrop of GST A53 Elutions
• Nanodrop of GST Elutions
• Nanodrop of MBP lnqZA hybrid Elutions
• Ran an SDS Page gel to determine whether or not the protein was being synthesized/ where in the purification process it was being collected
• Tested elutions of GST Epi and MBP lnqZA
8/17:
• Protein purified MBP lnqZE hybrid, two different samples of MBP lnqZA hybrid, and MBP Lnq ZQ hybrid
8/18:
• Nandodrop of MBP lnqZE Elutions
• Nanodrop of sample 1 of MBP lnqZA
• Nanodrop of sample 2 of MBP lnqZA