Team:UNOTT/Results

STEP 1: Create guideRNA Plasmid

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STEP 2: Create Reporter Plasmid

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STEP 3: Create Promoter Library

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STEP 4: Random Ligations

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STEP 5: Freeze drying & Revival

Here are the results of our freeze-dried cells revival experiments. With these experiments, we want to show that different storage do not have an effect on the revival of the cells within our key.

STEP 6: CRISPRi & guideRNA efficiency

This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, or with a non-targeting gRNA plasmid.

RESULTS: