Team:Virginia/Part Collection
Part Collection
This part collection includes reporter circuits of newly introduced promoters, functional nitrification pathway enzymes coding sequence, and the final combination of promoters and protein coding sequence. Future team can build more complex nitrification circuits with additional desired functions based on our part collection. This collection should serve as a base for further research related to nitrification and denitrification pathways, as well as be considered as a significant step taken towards an ideally constructed circuit.
Basic Parts
- BBa_K2272000 - a promoter that is maximally induced under microaerobic condition and regulates the expression of VHb, a bacterial hemoglobin that acts as a terminal oxidase.
- BBa_K2272001 - a promoter that regulates the production of MnSOD (mitochondrial antioxidant manganese superoxide dismutase), which detoxify radical oxygen produced by mitochondrial respiration.
- BBa_K2272002 - this part encodes Hydroxylamine Oxidoreductase (HAO), which catalyzes the oxidation of hydroxylamine to nitrite
- BBa_K2272003 - this part encodes two cytochromes that facilitate electron transport during the nitrification process
- BBa_K2272004 - this sequence from Paracoccus denitrificans codes for a signal peptide that will export the tagged protein to the periplasm of the cell
- BBa_K2272005 - this sequence from Nitrosomonas europaea codes for a signal peptide that exports the tagged protein to the periplasm of the cell
Composite Parts
- BBa_K2272007 - a reporter circuit for basic part BBa_K2272005
- BBa_K2272009 - another reporter circuit for BBa_K2272005
- BBa_K2272010 - a reporter circuit for basic part BBa_K2272004
- BBa_K2272008 - another reporter circuit for BBa_K2272004
- BBa_K22720011 - a reporter circuit for BBa_K2272005
- BBa_K2272006 - a reporter circuit for BBa_K2272004
- BBa_K2272012 - a reporter circuit for BBa_K2272001
- BBa_K2272013 - a reporter circuit for BBa_K2272000
- BBa_K2272014 - Cytochrome cycA and cycX coding sequence regulated by arabinose-inducible promoter from the distribution kit, pBAD.
- BBa_K2272015 - Cytochrome cycA and cycX coding sequence regulated by the microaerobic conditioned promoter VHb.
- BBa_K2272016 - Cytochrome cycA and cycX coding sequence regulated by hypoxic conditioned promoter, MnSOD.
- BBa_K2272017 - Coding sequence of hydroxylamine oxidoreductase regulated by hypoxic conditioned promoter, MnSOD.
- BBa_K2272018 - Coding sequence of hydroxylamine oxidoreductase regulated by arabinose-inducible promoter, pBAD.
- BBa_K2272019 - a composite version of BBa_K1067002 and BBa_K1067004
- BBa_K2272020 - a composite version of BBa_K1067002 and BBa_K1067004
