Team:Wageningen UR/Achievements
Achievements
This year's Wageningen iGEM team has been working hard and while doing so has kept the medal requirements in mind. Below each medal, a list can be found which completes the criteria that have to be met to earn the medals.
- Advanced models were created and used to get insight into the quorum sensing and reporter system parts of our project.
- Integrated Human Practices:
- We consulted with experts from the Dutch National Institute of Public Health and the Environment (RIVM), the corporate Foundation for Innovative New Diagnostics in Belgium, workers of the Médecines sans Frontières (MSF/Doctors without Borders) organization, a philosophy professor and many more. With the insights gained from experts in the field of diagnostics and people from MSF in Africa and Brazil we were able to improve the diagnostic device to suit the requirements of such a device. We were also able to take the biosafety aspect of our project into account and even consider the ethical dillemma's that arise when implementing a new diagnostic device.
- We improved BioBricks BBa_I715022 & BBa_I715023 by changing the incorrect split site AA155 to the correct split site AA168 BBa_K2387055.
- During our project we demonstrated that Mantis is viable in blood serum dilutions, and that fluorescence can indeed be measured in blood serum. Furthermore, we performed experiments to consistently air-dry our cells, and we combined several wet-lab project to perfect the functionality of Mantis.
- Validated the functionality of the following submitted BioBricks:
- We collaborated with the Utrecht and TU Delft iGEM teams to verify parts of our systems.
- Organized and hosted the BeNeLux iGEM meet-up.
- Participated and completed a RIVM project on biosafety, which was rewarded with a grant.
- We thoroughly looked into the ethical aspects of implementing a new diagnostic.
- Since biosafety is a concern when working with GMO's we looked into options of biocontainment and did a literature research on risk and safety assessment.
| T. brucei antigens | ||
|---|---|---|
| BioBrick Code | Part Name | Designer |
| BBa_K2387060 | Double tagged ISG64 of T. brucei gambiense | Linda |
| BBa_K2387061 | Double tagged ISG65 of T. brucei gambiense | Linda |
| Cpx System | ||
|---|---|---|
| BioBrick Code | Part Name | Designer |
| BBa_K2387014 | Cpx system reporter + CpxP inhibitor | Stijn |
| BBa_K2387017 | Cpx system reporter + CpxP-Affinity molecule inhibitor | Stijn |
| BBa_K2387018 | Cpx system reporter + Affinity molecule-CpxP inhibitor | Stijn |
| BBa_K2387022 | Cpx system reporter + tethered CpxP | Stijn |
| BBa_K2387023 | Cpx system reporter + tethered CpxP-Affinity molecule | Stijn |
| BBa_K2387024 | Cpx system reporter + tethered Affinity molecule-CpxP | Stijn |
| Reporters and Split Reporters | ||
|---|---|---|
| BioBrick Code | Part Name | Designer |
| BBa_K2387001 | Monomeric chromoprotein anm2CP controlled by inducible araC/pBAD promoter | José Manuel |
| BBa_K2387045 | mVenus controlled by inducible araC/pBAD promoter | José Manuel |
| BBa_K2387046 | Split mVenus controlled by inducible araC/pBAD promoter | José Manuel |
| BBa_K2387047 | sfGFP controlled by inducible araC/pBAD promoter | José Manuel |
| BBa_K2387048 | Split sfGFP controlled by inducible araC/pBAD promoter | José Manuel |
| BBa_K2387052 | mCerulean controlled by inducible araC/pBAD promoter | José Manuel |
| BBa_K2387053 | Split mCerulean controlled by inducible araC/pBAD promoter | José Manuel |
| BBa_K2387054 | mRFP controlled by inducible araC/pBAD promoter | José Manuel |
| BBa_K2387055 | Split mRFP controlled by inducible araC/pBAD promoter | José Manuel |
| BBa_K2387003 | eYFP controlled by inducible araC/pBAD promoter | Bart |
| BBa_K2387007 | eYFPn [1-154] controlled by inducible araC/pBAD promoter | Bart |
| BBa_K2387008 | eYFPc [155-238] controlled by inducible araC/pBAD promoter | Bart |
| BBa_K2387009 | eYFPn [1-154] and eYFPc[155-238] controlled by inducible araC/pBAD promoter | Bart |
| BBa_K2387062 | eYFP[155-238]-cLeucine Zipper fusion controlled by inducible araC/pBAD promoter | Bart |
| BBa_K2387063 | eYFP[1-154]-nLeucine Zipper fusion controlled by inducible araC/pBAD promoter | Bart |
| BBa_K2387065 | eYFP[1-154]-nLeucine Zipper and eYFP[155-238]-cLeucine Zipper fusions controlled by inducible araC/pBAD promoter | Bart |
| Cpx + BiFC | ||
|---|---|---|
| BioBrick Code | Part Name | Designer |
| BBa_K2387010 | CpxA-eYFPn fusion controlled by inducible araC/pBAD promoter | Bart |
| BBa_K2387029 | CpxR-mVenusn and CpxR-mVenusc fusions controlled by inducible araC/pBAD promoter | Bart |
| BBa_K2387030 | CpxR-eYFPc fusion controlled by inducible araC/pBAD promoter | Bart |
| BBa_K2387031 | CpxR-eYFPn fusion controlled by inducible araC/pBAD promoter | Bart |
| BBa_K2387032 | CpxR-eYFPn and CpxR-eYPFc fusions controlled by inducible araC/pBAD promoter | Bart |
| BBa_K2387074 | TEV protease fused to CpxR controlled by inducible araC/pBAD promoter | Bart |
| Quorum Sensing System | ||
|---|---|---|
| BioBrick Code | Part Name | Designer |
| BBa_K2387066 | Colicin E7 lytic mechanism controlled by inducible araC/pBAD promoter | Natalia |
| BBa_K2387068 | T4 Holin lytic mechanism controlled by inducible araC/pBAD promoter | Natalia |
| BBa_K2387069 | T4 Endolysin lytic mechanism controlled by inducible araC/pBAD promoter | Natalia |
| BBa_K2387070 | Repressible aiiA | Natalia |
| BBa_K2387071 | Constitutive TEV protease | Natalia |
| BBa_K2387072 | GFP quenched by REACh2 | Natalia |
| BBa_K2387073 | Inducible GFP quenched by REACh2 | Natalia |
- Registered for iGEM, had a great summer, and will attend the Giant Jamboree.
- We met all deliverables requirements and submitted 36 new BioBricks.
- Created an Attributions page with clear attribution of each aspect of our project.
- We participated in the InterLab Measurement Study.
