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<i class="fa fa-flask" style="font-size:px;color:white;"></i></br>Characterization of a Thermal</br>Actuator and Calcium-Sensitive Reporter in</br>Saccharomyces cerevisiae</br></br></center></font></p>
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<font face="Arial" size="6"><center>Project Description</center></font><hr>
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    <div class="col-sm-6"><font face="Arial" size="4">Our current work was inspired by the ability of a thermosensitive ion channel, known as TRPV1, to import calcium into the cell in response to temperature changes. This process could one day be used to provide a method of <i>In-Silico</i> control of genetic circuits in synthetic biology. Our project explores the characterization of a calcium-sensitive reporter in Saccharomyces cerevisiae to monitor gene expression levels within a range of temperatures. 
</br></br><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Description">To learn more about our project, click here.</a></font></div>
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<font face="Arial" size="6"><center>Project Aims</center></font><hr>
</br><font face="Arial" size="4">For our project this year we focused on characterizing the optimal extracellular calcium conditions to induce the expression of our calcium sensitive reporters. We are working with two reporter proteins that provide quick maturation rates and bright fluorescence within yeast so they will make for useful output indicators for <i>In-Silico</i> control in future work.

</br></br>We also built on work from previous iGEM teams to further develop a deterministic model for the calcium pathway in yeast that will activate our reporter genes. Additionally, we developed a stochastic model to predict the nature of ion channel gating in response to temperature change. These models will help guide future work with TRPV1 as a thermal actuator in yeast.  

</br></br><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Results">To view our results, click here.</a>
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</br><font face="Arial" size="4">(<b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range.)</font></br></br></br>
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    <div class="col-sm-4"><font face="Arial" size="6">About Us</font><hr></br><font face="Arial" size="3">The Lubbock_TTU Team is part of a student organization at Texas Tech University known as iGEM Raiders. This year, the iGEM Raiders team is composed of 11 undergraduate and graduate students. </br></br><a href="https://2017.igem.org/Team:LUBBOCK_TTU/Team">To learn more about our team, click here.</a></font></div>
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