Difference between revisions of "Team:UNOTT/Design2"

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<p>These bricks are formed from a random soup of characterised promoters "P", a reporter gene fluorescent protein, and a random terminator. This uses BSA I sites already digested previously into the DNA in order to ligate randomly in the correct order to create a random, yet purposeful and functional fluorescent signal.
 
<p>These bricks are formed from a random soup of characterised promoters "P", a reporter gene fluorescent protein, and a random terminator. This uses BSA I sites already digested previously into the DNA in order to ligate randomly in the correct order to create a random, yet purposeful and functional fluorescent signal.
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<h3 style="text-align: center;"><span style="color: #D74214;"> Plasmid Design </span></h3>
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<h3 style="text-align: center;"><span style="color: #D74214;"> Brick stitching </span></h3>
 
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<p style="text-align: center;"><img src="https://static.igem.org/mediawiki/2017/2/29/UNOTT2017-DCBAbricks.png" alt="" width="500" height="128" />&nbsp;</p>
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<p>These bricks are then stitched together via amplifying each randomly assembled brick through common amplification sites and then cutting them using a set of restriction enzymes which give each plasmid a specific order of bricks, depending on which are cut and then ligated together. As shown.
 
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Revision as of 00:12, 14 September 2017

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KEY PLASMID DESIGN

 

 

Random Brick formation (Components of plasmid)

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These bricks are formed from a random soup of characterised promoters "P", a reporter gene fluorescent protein, and a random terminator. This uses BSA I sites already digested previously into the DNA in order to ligate randomly in the correct order to create a random, yet purposeful and functional fluorescent signal.

Brick stitching

___________________________

 

These bricks are then stitched together via amplifying each randomly assembled brick through common amplification sites and then cutting them using a set of restriction enzymes which give each plasmid a specific order of bricks, depending on which are cut and then ligated together. As shown.