To predict theoretical acetaminophen production, we calculated the amount of its precursor, chorismate, by quantifying its main products, the aromatic amino acids phenylalanine, tyrosine, and tryptophan. Since no amino acid composition data was available for Synechococcus, we started by using literature data for the similar cyanobacteria species Spirulina found that between 11 and 13.6 percent of amino acids were aromatics by mass, or between 6.5 and 7.7 molar percent of total protein. To further verify our organism's amount of acetaminophen precursor, we ran both the genome and ribosomal protein sequences through a custom Python program converting codons to amino acids and calculating aromatic amino acid molar percentages which resulted in 9.3% and 5.14% respectively.

Using our sequence generated median chorismate value of 9.3% and the assumption that our enzymes would take a third of the chorismate precursor, we got an estimate for acetaminophen concentration of between 17.5mg and 1.29 mg per gram dried biomass. These numbers show that there is likely enough precursor and that acetaminophen production should be within a measurable range.

To understand the production capacity of our organism, we aggregated growth data from published papers and all of our lab’s growth data. Using limited logistic growth curves and linear algebra to fit our equation, we modelled dried biomass and cell count per time, with the additional dependent variables of temperature, light intensity, and starter culture density.