Difference between revisions of "Team:UCSC/Attributions"

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For the past four years, undergraduates at the University of California, Santa Cruz (UCSC) have participated in iGEM. This year, the team is composed of students studying Biomolecular Engineering, Bioinformatics, Bioelectronics, Biochemistry, Molecular, Cellular, and Developmental (MCD) Biology, and Chemical Engineering. This wide array of disciplines is crucial for the creative process behind using synthetic biology to advance solutions to problems. Modern engineering demands collaboration between individuals and pushes them to think critically and work efficiently. Through synthetic biology, the 2017 UCSC iGEM team is creating effective solutions and developing models for future success.
 
For the past four years, undergraduates at the University of California, Santa Cruz (UCSC) have participated in iGEM. This year, the team is composed of students studying Biomolecular Engineering, Bioinformatics, Bioelectronics, Biochemistry, Molecular, Cellular, and Developmental (MCD) Biology, and Chemical Engineering. This wide array of disciplines is crucial for the creative process behind using synthetic biology to advance solutions to problems. Modern engineering demands collaboration between individuals and pushes them to think critically and work efficiently. Through synthetic biology, the 2017 UCSC iGEM team is creating effective solutions and developing models for future success.
 
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<h3>General Support</h3>
 
<h3>General Support</h3>
 
We would like to thank Dr. Hartzog for his work to help iGEM BME188 coursework satisfy the MCD disciplinary communication requirement.  
 
We would like to thank Dr. Hartzog for his work to help iGEM BME188 coursework satisfy the MCD disciplinary communication requirement.  
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</br>
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<b>Elliot Roth</b>, Founder and CEO at Spira
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<p>Spoke with us about his experience working with Spirulina and provided insight into working with the genetics of Spirulina.</p>
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Dr. Susan S. Golden, Professor at Howard Hughs Medical Institute, Director at the Center for Circadian Biology, Chancellor’s Associates Chair (III) in Molecular Biology, Distinguished Professor at University of California, San Diego 
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Provided us with pAM1573
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</br>
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Dr. Donald Bryant, Ernest C. Pollard Professor in Biotechnology, Professor of Biochemistry and Molecular Biology at Penn State
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Provided us with Synechococcus sp. PCC 7002 and advice regarding the best plates to grow cyanobacteria.
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</br>
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Dr. J. Christopher Anderson, Associate Professor, Bioengineering at the University of California, Berkeley
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Pioneered Acetaminophen production in E. coli and Saccharomyces cerevisiae and is working with UCSC iGEM to extend the IP to photosynthetic organisms.
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UCSC iGEM 2016
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Members of the UCSC iGEM 2016 team provided us with valuable advice on project planning and execution.
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</br>
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Dr. Rohinton T. Kamakaka & Members of the Kamakaka Lab, Department of Molecular, Cell, and Developmental Biology at the University of California, Santa Cruz
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Provided our team with lab materials, access to UV gel dock (check name), access to Thermo ScientificTM NanoDropTM 2000/2000c (check), and providing valuable suggestions and guidance on troubleshooting experiments.
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</br>
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Dr. Anne M. Ruffing, Senior Member of the Technical Staff, Bioenergy and Defense Technologies Department, Sandia National Labs
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Spoke with us about her experience working with cyanobacteria, specifically Synechococcus elongatus PCC 7942. Dr. Ruffing advised about optimizing growth conditions of our S. elongatus cultures.
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</br>
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Dr. Chris Vollmers & Members of the Vollmers Lab (hyperlink: https://vollmerslab.soe.ucsc.edu/ ), Department of Biomolecular Engineering, University of California, Santa Cruz
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Donated pipet tips and gloves and provided suggestions for troubleshooting issues with gel extraction.     
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</br>     
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Dr. Chad Saltikov & Members of the Saltikov Lab, Department of Environmental Toxicology, University of California, Santa Cruz
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Donated DPN1.
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<b>Haussler Lab</b> <a href="https://hausslergenomics.ucsc.edu/"> Genomics Institue </a>, University of California, Santa Cruz
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Donated gloves, gel extraction kits, and provided us with temporary lab space.
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</br>
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<b>Dr. Olof Einarsdottir</b>, Professor, Physical & Biological Sciences—Chemistry & Biochemistry Department
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        Provided us with advice on modeling.
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</br>
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<b>Dr. Grant Hartzog & Members of the Hartzog Lab</b>, Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz
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        Donated monobasic potassium phosphate.
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</br>       
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<b>Heather Tyra<b/>, Integrated DNA Technologies, Product Support Specialist for the IDT Synthetic Biology Group
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Assisted our team in ordering the parts needed for the riboswitch component of our project.
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</br>
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<b>UC Berkeley DNA Sequencing Facility</b>
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Sequenced our samples of pAM2991, pAM1573, ssuE, 4ABH, bluB, and nhoA.
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</br>
  
 
<h3>Project support and advice</h3>
 
<h3>Project support and advice</h3>
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<h3>Presentation coaching</h3>
 
<h3>Presentation coaching</h3>
 
<h3>Human Practices support</h3>
 
<h3>Human Practices support</h3>
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<h2>Information about UCSC iGEM</h2>
 
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Questions to answer
 
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    <li>Does your institution teach an iGEM or synthetic biology course?</li>
 
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    <li>When did you start this course?</li>
 
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    <li>Are the syllabus and course materials freely available online?</li>
 
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<li>When did you start your brainstorming? When did you start in the lab? When did you start working on your project?</li>
 
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<p>At UCSC, participation in iGEM satisfies the senior design exit requirement for bioengineering majors and is given class credit by two 5-week classes, BME 188a BME188b, starting in late june (june 26th?) and running until early septemeber (exact date ? ). There is a paper due at the end of each summer class session describing the work that we have done. Brainstorming and discussion about potential projects started with the hard work of our co-captains in late Fall 2016 and early Winter 2017. We held weekly journal clubs to brainstorm ideas during Winter 2017 and picked team members in late Winter 2017 through early Spring 2017. After solidifyng team members, we held meetings once or twice a week to further discuss direction of the project. When the first summer session started (BME 188a), we had a change in direction from producing aspirin using lactobacillus to producing vitamins using spirulina. We soon realized it was very difficult to obtain a sequenced spirulina strain, and that genetic modification is very difficult, and not well documented. We went with a different photosynthetic organism, Synechococcus, to use as a model for spirulina, and obtained it by week 3 of BME188a. We started in the lab around this time. </p>
 
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Revision as of 23:23, 22 September 2017

Attributions

Attributions

We would like to acknowledge and thank all of the people who have helped make this project possible. For the past four years, undergraduates at the University of California, Santa Cruz (UCSC) have participated in iGEM. This year, the team is composed of students studying Biomolecular Engineering, Bioinformatics, Bioelectronics, Biochemistry, Molecular, Cellular, and Developmental (MCD) Biology, and Chemical Engineering. This wide array of disciplines is crucial for the creative process behind using synthetic biology to advance solutions to problems. Modern engineering demands collaboration between individuals and pushes them to think critically and work efficiently. Through synthetic biology, the 2017 UCSC iGEM team is creating effective solutions and developing models for future success.


General Support

We would like to thank Dr. Hartzog for his work to help iGEM BME188 coursework satisfy the MCD disciplinary communication requirement.
Elliot Roth, Founder and CEO at Spira

Spoke with us about his experience working with Spirulina and provided insight into working with the genetics of Spirulina.

Dr. Susan S. Golden, Professor at Howard Hughs Medical Institute, Director at the Center for Circadian Biology, Chancellor’s Associates Chair (III) in Molecular Biology, Distinguished Professor at University of California, San Diego Provided us with pAM1573
Dr. Donald Bryant, Ernest C. Pollard Professor in Biotechnology, Professor of Biochemistry and Molecular Biology at Penn State Provided us with Synechococcus sp. PCC 7002 and advice regarding the best plates to grow cyanobacteria.
Dr. J. Christopher Anderson, Associate Professor, Bioengineering at the University of California, Berkeley Pioneered Acetaminophen production in E. coli and Saccharomyces cerevisiae and is working with UCSC iGEM to extend the IP to photosynthetic organisms.
UCSC iGEM 2016 Members of the UCSC iGEM 2016 team provided us with valuable advice on project planning and execution.
Dr. Rohinton T. Kamakaka & Members of the Kamakaka Lab, Department of Molecular, Cell, and Developmental Biology at the University of California, Santa Cruz Provided our team with lab materials, access to UV gel dock (check name), access to Thermo ScientificTM NanoDropTM 2000/2000c (check), and providing valuable suggestions and guidance on troubleshooting experiments.
Dr. Anne M. Ruffing, Senior Member of the Technical Staff, Bioenergy and Defense Technologies Department, Sandia National Labs Spoke with us about her experience working with cyanobacteria, specifically Synechococcus elongatus PCC 7942. Dr. Ruffing advised about optimizing growth conditions of our S. elongatus cultures.
Dr. Chris Vollmers & Members of the Vollmers Lab (hyperlink: https://vollmerslab.soe.ucsc.edu/ ), Department of Biomolecular Engineering, University of California, Santa Cruz Donated pipet tips and gloves and provided suggestions for troubleshooting issues with gel extraction.
Dr. Chad Saltikov & Members of the Saltikov Lab, Department of Environmental Toxicology, University of California, Santa Cruz Donated DPN1.
Haussler Lab Genomics Institue , University of California, Santa Cruz Donated gloves, gel extraction kits, and provided us with temporary lab space.
Dr. Olof Einarsdottir, Professor, Physical & Biological Sciences—Chemistry & Biochemistry Department Provided us with advice on modeling.
Dr. Grant Hartzog & Members of the Hartzog Lab, Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz Donated monobasic potassium phosphate.
Heather Tyra, Integrated DNA Technologies, Product Support Specialist for the IDT Synthetic Biology Group Assisted our team in ordering the parts needed for the riboswitch component of our project.
UC Berkeley DNA Sequencing Facility Sequenced our samples of pAM2991, pAM1573, ssuE, 4ABH, bluB, and nhoA.

Project support and advice

Lab support

Difficult technique support

Project advisor support

Wiki support

Presentation coaching

Human Practices support

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S P O N S O R S
S O C I A L
C O N T A C T
Jack Baskin School of Engineering
University of California, Santa Cruz
1156 High Street
Santa Cruz, California, 95064