When developing Key. coli, we found it was important to mathematically model possible situations in order to investigate the effects of different situations that we might encounter throughout different stages of development as well as during implementation. Software was developed to compare the fluorescence levels of the key colony with the mother colony to check if there was a high enough degree of similarity.       This information was used by the wet lab to assist them by informing them in what to expect. This was done through the use of programming to create visual graphs and simulations, as well as development of tools to allow for comparison between fluorescence levels without needing to actually create more synthetic organisms. Another advantage is that this is far quicker than creating these results in the lab. The source code for these models can be accessed from our GitHub page The models were not perfect at first: refinement from lab results helped to optimize and correct the models.