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<p> psb1a3: 75ng/ul </p> | <p> psb1a3: 75ng/ul </p> | ||
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In preparation Alyssa and I rehydrated 2 backbones from iGEM, the PSB1C3 and PSB1A3. The C3 was from plate 7 location O,23 and the A3 was from plate 4 location H,2. Will let the plates grow overnight in the incubator and make MP samples, in triplicates of each, tomorrow.
DNA extraction from kit and rehydration protocol:
To use the DNA in the Distribution Kit, follow these instructions:
Note: There is an estimated 2-3ng of DNA in each well, following this protocol, assume that you are transforming with 200- 300pg/µL
* To know which antibiotics to use, look at the plasmid that the part is in. The naming scheme for plasmids is specifically designed to indicate antibiotic resistance.
7/20/2017 - something went wrong with the process, the plates to not show sufficient growth. Tests and plating will be redone today.
You can see what others teams have done to organize their notes:
