Difference between revisions of "Team:NWU-CHINA/Safety"

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<h1> Safety </h1>
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h1>Safety</h1>
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        <p>&nbsp;&nbsp;In biology research, especially researches using microorganism, biosafety is of almighty importance to researchers, environment, and the public. As far as we are concerned, biosafety  can be divided into two parts, safety operation and BSL of the lab.</p>
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    <h3>Our chassis</h3>
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        <p>&nbsp;&nbsp;We chose E.coli and P.a as our chassis. As we all know, E.coli is a BSL&minus;1 chassis, so we just do experiment on bench with E.coli. As for P.a, it is a kind of conditional pathogen which can cause disease when people immune decrease, so it is commonly in hospital. Disease  caused by this bacteria can be cured. However, as P.a is a BSL-2 chassis, we do experiment in biosafety cabinet with P.a. Besides, when new team member join us, we will train there operation skills and widespread biosafety knowledge to them.</p>
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        <p>&nbsp;&nbsp;Our part all include coding gene about alkane degradation. It is not harmful to plants or animals.</p>
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    <h3>Safety practice</h3>
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        <p>&nbsp;&nbsp;Before our experiment, we will put on lab coat, gloves and mask to protect us and our sample. During our experiment, we will use alcohol lights,UV illuminator and DNA gel electrophoresis. In this time, we put on gloves and glasses to protect ourselves from UV and TAE. After our experiment, we will sterilize items in contact with bacteria. When we use autoclaves, advisor will be with us to ensure our safety.</p>
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<p>&nbsp;&nbsp;What&prime;s more, we also checked our antibiotic is safe enough to use.</p>
  
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Revision as of 07:05, 14 October 2017

Safety

  In biology research, especially researches using microorganism, biosafety is of almighty importance to researchers, environment, and the public. As far as we are concerned, biosafety can be divided into two parts, safety operation and BSL of the lab.

Our chassis

  We chose E.coli and P.a as our chassis. As we all know, E.coli is a BSL−1 chassis, so we just do experiment on bench with E.coli. As for P.a, it is a kind of conditional pathogen which can cause disease when people immune decrease, so it is commonly in hospital. Disease caused by this bacteria can be cured. However, as P.a is a BSL-2 chassis, we do experiment in biosafety cabinet with P.a. Besides, when new team member join us, we will train there operation skills and widespread biosafety knowledge to them.

  Our part all include coding gene about alkane degradation. It is not harmful to plants or animals.

Safety practice

  Before our experiment, we will put on lab coat, gloves and mask to protect us and our sample. During our experiment, we will use alcohol lights,UV illuminator and DNA gel electrophoresis. In this time, we put on gloves and glasses to protect ourselves from UV and TAE. After our experiment, we will sterilize items in contact with bacteria. When we use autoclaves, advisor will be with us to ensure our safety.

  What′s more, we also checked our antibiotic is safe enough to use.