Difference between revisions of "Team:TP-CC San Diego/LabBook"

Line 85: Line 85:
 
       </p>
 
       </p>
 
       <ul>  
 
       <ul>  
−
           <li>primers arrived</li>
+
           <li>Primers arrived</li>
 
       </ul>
 
       </ul>
 
   </div>
 
   </div>
Line 92: Line 92:
 
     <div class = "description">  
 
     <div class = "description">  
 
         <p>  
 
         <p>  
−
             <b>SUMMARY of August 1: </b>  
+
             <b>SUMMARY of August 1st: </b>  
 
         </p>
 
         </p>
 
         <ul>
 
         <ul>
Line 99: Line 99:
 
     </div>
 
     </div>
  
 +
<h3 class = "day"> DAY 5 </h3>
 +
 
 +
    <div class = "description">
 +
        <p>
 +
            <b>SUMMARY of August 2nd: </b>
 +
        </p>
 +
        <ul>
 +
            <li>Received oligos </li>
 +
        </ul>
 +
    </div>
 +
 +
<h3 class = "day"> DAY 6 </h3>
 +
 
 +
    <div class = "description">
 +
        <p>
 +
            <b>SUMMARY of August 7th: </b>
 +
        </p>
 +
        <ul>
 +
            <li>Digestion of lentiviral CRISPR plasmid </li>
 +
            <li>Gel purification digested plasmid using QIAquick Gel Extraction Kit and eluted in buffer EB</li>
 +
            <li>There should be two bands on the gel, one at 2kb which should not be purified. Purify only the larger band</li>
 +
        </ul>
 +
    </div>
 +
<h3 class = "day"> DAY 7 </h3>
 +
 
 +
    <div class = "description">
 +
        <p>
 +
            <b>SUMMARY of August 9th: </b>
 +
        </p>
 +
        <ul>
 +
            <li>Anneal oligo pairs </li>
 +
            <li>Ligation and transformation<li>
 +
        </ul>
 +
    </div>
 +
 +
<h3 class = "day"> DAY 8 </h3>
 +
 
 +
    <div class = "description">
 +
        <p>
 +
            <b>SUMMARY of August 10th: </b>
 +
        </p>
 +
        <ul>
 +
            <li>No colonies grew on carb antibiotic plates </li>
 +
        </ul>
 +
    </div>
 
</div>
 
</div>
  

Revision as of 04:22, 15 October 2017


Lab Notebook

DAY 1

SUMMARY of July 17th:

  • DNA extraction from GBM39 cells
  • Agarose gel electrophoresis to confirm DNA

DAY 2

SUMMARY of July 18th:

  • qPCR primer design and order

DAY 3

SUMMARY of July 21:

  • Primers arrived

DAY 4

SUMMARY of August 1st:

DAY 5

SUMMARY of August 2nd:

  • Received oligos

DAY 6

SUMMARY of August 7th:

  • Digestion of lentiviral CRISPR plasmid
  • Gel purification digested plasmid using QIAquick Gel Extraction Kit and eluted in buffer EB
  • There should be two bands on the gel, one at 2kb which should not be purified. Purify only the larger band

DAY 7

SUMMARY of August 9th:

  • Anneal oligo pairs
  • Ligation and transformation

DAY 8

SUMMARY of August 10th:

  • No colonies grew on carb antibiotic plates