Difference between revisions of "Team:TP-CC San Diego/LabBook"

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         <ul>
 
         <ul>
 
             <li>No colonies grew on carb antibiotic plates </li>
 
             <li>No colonies grew on carb antibiotic plates </li>
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        </ul>
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    </div>
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<h3 class = "day"> DAY 4 </h3>
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    <div class = "description">
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        <p>
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            <b>SUMMARY of August 11th: </b>
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        </p>
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        <ul>
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            <li>Used same,r </li>
 
         </ul>
 
         </ul>
 
     </div>
 
     </div>

Revision as of 04:26, 15 October 2017


Lab Notebook

DAY 1

SUMMARY of July 17th:

  • DNA extraction from GBM39 cells
  • Agarose gel electrophoresis to confirm DNA

DAY 2

SUMMARY of July 18th:

  • qPCR primer design and order

DAY 3

SUMMARY of July 21:

  • Primers arrived

DAY 4

SUMMARY of August 1st:

DAY 5

SUMMARY of August 2nd:

  • Received oligos

DAY 6

SUMMARY of August 7th:

  • Digestion of lentiviral CRISPR plasmid
  • Gel purification digested plasmid using QIAquick Gel Extraction Kit and eluted in buffer EB
  • There should be two bands on the gel, one at 2kb which should not be purified. Purify only the larger band

DAY 7

SUMMARY of August 9th:

  • Anneal oligo pairs
  • Ligation and transformation

DAY 8

SUMMARY of August 10th:

  • No colonies grew on carb antibiotic plates

DAY 4

SUMMARY of August 11th:

  • Used same,r