Difference between revisions of "Team:IISc-Bangalore/Description"

(Undo revision 187793 by Raj Magesh (talk))
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<h1>About iFLOAT</h1>
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<p>Tell us about your project, describe what moves you and why this is something important for your team. <br>
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iFLOAT is a novel protein purification strategy that aims to reduce the exorbitant cost of recombinant protein synthesis by targeting the most expensive stage: downstream processing. By tagging the recombinant protein to a cyanobacterial gas vesicle protein - a "floater" - the desired protein can be purified using a standardized protocol.
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<h5>What should this page contain?</h5>
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<ul>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<li>References and sources to document your research.</li>
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<li>Use illustrations and other visual resources to explain your project.</li>
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</ul>
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            <ol>
 
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                <li>iFLOAT</li>
 
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                <li>Gas vesicles</li>
 
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                <li>Bioengineering</li>
 
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                <li>The Problem</li>
 
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                <li>Our Solutions</li>
 
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            </ol>
 
 
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</div>
  
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<h1>iFLOAT</h1>
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<h5>Advice on writing your Project Description</h5>
  
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<h2>a multifaceted approach to cluster bioengineered gas vesicles in vitro and enhance their flotation</h2>
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<p>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
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<p>Gas vesicles (GVs) are hollow protein nanostructures synthesized by phototrophic haloarchaea and cyanobacteria to regulate their flotation in aquatic habitats. Bioengineered GVs have been genetically modified for diverse purposes including ultrasonic molecular imaging, gauging cellular turgor pressures, and vaccine delivery — harnessing unique acoustic, mechanical, and surface properties of GVs — but none of their current applications exploits their most fundamental characteristic: buoyancy. </p>
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<p>
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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</p>
  
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<p>Our modelling indicates that clusters of GVs float several orders of magnitude better than individual GVs, as buoyancy scales with volume while Stokes’ drag scales with effective radius. Our project iFLOAT aims to improve the flotation of gas vesicles by clustering them using three distinct methods — charge-based flocculation, biotin-streptavidin interaction, and SpyCatcher-SpyTag heterodimerization — and simultaneously develop robust, reproducible flotation assays. Potential future applications of buoyant clusters of bioengineered gas vesicles include bioremediation of oil spills and flotation-based separation and purification of specific targets from mixtures.</p>
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<h5>References</h5>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
  
 
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<h5>Inspiration</h5>
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<p>See how other teams have described and presented their projects: </p>
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<ul>
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<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
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<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
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</ul>
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</div>
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</html>

Revision as of 18:23, 16 October 2017

About iFLOAT

Tell us about your project, describe what moves you and why this is something important for your team.
iFLOAT is a novel protein purification strategy that aims to reduce the exorbitant cost of recombinant protein synthesis by targeting the most expensive stage: downstream processing. By tagging the recombinant protein to a cyanobacterial gas vesicle protein - a "floater" - the desired protein can be purified using a standardized protocol.

What should this page contain?
  • A clear and concise description of your project.
  • A detailed explanation of why your team chose to work on this particular project.
  • References and sources to document your research.
  • Use illustrations and other visual resources to explain your project.
Advice on writing your Project Description

We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.

Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.

References

iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.

Inspiration

See how other teams have described and presented their projects: