Difference between revisions of "Team:SSTi-SZGD/Description"

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<p>The level of pesticide usage in China is 2.5 times above the world average. In June 2016, the total output of chemical pesticides in China was around 3.347 million tons, up by 7.17% compared with the same period of 2015. A range of chemical and physical technologies have been widely applied in the degradation of pesticide residues. These techniques are of high in accuracy and easy to be manipulated characteristics. However, the shortfalls come with high in cost, harmful to non-target organisms, food, plants and soils, and likely to cause secondary pollution, etc.</p>
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The level of pesticide usage in China is 2.5 times above the world average. In June 2016, the total output of chemical pesticides in China was around 3.347 million tons, up by 7.17% compared with the same period of 2015. The deterioration effects of long-term and overdose usage of pesticide in soil include soil erosion, soil contamination, water pollution, organic pollution, reducing biodiversity, etc. A range of chemical and physical technologies have been widely applied in the degradation of pesticide residues. These techniques are of high in accuracy and easy to be manipulated characteristics. However, the shortfalls come with high in cost, harmful to non-target organisms, food, plants and soils, and likely to cause secondary pollution, etc.  
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<p>The aim of this project is to combine optogenetics and biotechnology, by using genetically modified organisms as the core carriers, to develop a novel method for degrading pesticide residues in contaminated soil. We employed a novel light inducible/repressive system that can efficiently overexpress heterogenous proteins that are able to degrade pesticides chemicals organophosphorus and parathion-methyl pesticide, without the need of using toxic and expensive chemicals. In addition, we plan to develop a device that integrates monitoring and data-sharing functions that help applying this system to on-site pesticide residue detection and degradation. We hope to achieve a microbial degradation method with low toxicity, low cost and high efficiency which serves a positive role in maintaining ecological balance.</p>
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This year we aimed to to combine optogenetics and biotechnology, by using genetically modified organism (E.coli.) as a core carrier, to develop a novel method for degrading pesticide residues in contaminated soil. We employed a state-of-art light-regulated gene expression system (LightOFF) that can efficiently over-express heterogenous hydrolases that are able to degrade insecticide organophosphorus and fungicide carbendazim respectively, by using darkness as an inducer. This application can lead to two forms of products: enzyme and whole cell products, for efficiently degradation of pesticide residues in soil. In addition, we propose to develop an automatic spraying device to facilitate the usage of whole cell products in real world. Altogether we hope to develop a microbial degradation method with low toxicity, low cost and high efficiency that serves a positive role in maintaining ecological balance.
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Revision as of 01:01, 20 October 2017

SSTi-SZGD---Description

Guardian of the wheatland A product for the degradation of soil pesticide residues

Pesticide Residue Sweep--Why We Need it ?

The level of pesticide usage in China is 2.5 times above the world average. In June 2016, the total output of chemical pesticides in China was around 3.347 million tons, up by 7.17% compared with the same period of 2015. The deterioration effects of long-term and overdose usage of pesticide in soil include soil erosion, soil contamination, water pollution, organic pollution, reducing biodiversity, etc. A range of chemical and physical technologies have been widely applied in the degradation of pesticide residues. These techniques are of high in accuracy and easy to be manipulated characteristics. However, the shortfalls come with high in cost, harmful to non-target organisms, food, plants and soils, and likely to cause secondary pollution, etc.

How to work ?

This year we aimed to to combine optogenetics and biotechnology, by using genetically modified organism (E.coli.) as a core carrier, to develop a novel method for degrading pesticide residues in contaminated soil. We employed a state-of-art light-regulated gene expression system (LightOFF) that can efficiently over-express heterogenous hydrolases that are able to degrade insecticide organophosphorus and fungicide carbendazim respectively, by using darkness as an inducer. This application can lead to two forms of products: enzyme and whole cell products, for efficiently degradation of pesticide residues in soil. In addition, we propose to develop an automatic spraying device to facilitate the usage of whole cell products in real world. Altogether we hope to develop a microbial degradation method with low toxicity, low cost and high efficiency that serves a positive role in maintaining ecological balance.

A product for the degradation of soil pesticide residues Copyright © 2017 Lucky      power by  IGEM Team:SSTi-SZGD