Difference between revisions of "Team:Stony Brook/InterLab"

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<h1>InterLab</h1>
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<h3>Bronze Medal Criterion #4</h3>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range.
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For teams participating in the <a href="https://2017.igem.org/Competition/InterLab_Study">InterLab study</a>, all work must be shown on this page.  
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<li><a href="https://2017.igem.org/Team:Stony_Brook">Home</a></li>
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<li><a href="https://2017.igem.org/Team:Stony_Brook/Team">Team</a></li>
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                                      <header>
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<h2>InterLab</h2>
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<a href="#"><img src="https://static.igem.org/mediawiki/2017/b/bb/T--Stony_Brook--homepage-swords.png" style="text-align: center;width:250px;height:250px;"/></a>
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<p>This year, our team participated in the iGEM Fourth InterLab study. The objective of this study is to improve the measurement tools in order to obtain more reliable data within the field of synthetic biology. This year’s study aimed to improve the GFP and OD600 measurement protocol via the use of a plate reader. We were provided with 8 plasmids, including 2 controls and 6 test devices, to transform into DH5-alpha Escherichia Coli cells. We measured the fluorescence and absorbance with the Molecular Devices Microplate Reader SoftMax Pro 6.5.1.
 
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<header><h3>Part One</h3></header>
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<p>The initial procedure we carried out was to measure the OD600 reference point of LUDOX-S40. The absorbance readings are listed in the table below.</p>
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<a href="#"><img src="https://static.igem.org/mediawiki/2017/0/0f/T--Stony_Brook--interlab1.png" style= "text-align: center;width:500px;height:500px;"/></a>
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<header><h3>Part Two</h3></header>
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<p>The second part of the study was to construct a standard curve by plotting the fluorescence of fluorescin at different concentrations. The table below shows the values collected from the readings and the standard curve generated from the data. As expected, there is a positive correlation between the concentration of fluorescin in uM and the intensity of the fluorescence. </p>
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<a href="#"><img src="https://static.igem.org/mediawiki/2017/8/87/T--Stony_Brook--interlab2.png" style= "text-align: center;width:500px;height:500px;"/></a>
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<a href="#"><img src="https://static.igem.org/mediawiki/2017/7/76/T--Stony_Brook--interlab2-2.png" style= "text-align: center;width:500px;height:500px;"/></a>
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<header><h3>Part Three</h3></header>
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<p>The third part of the study is transform our 8 plasmids into DH5-alpha E. coli cells on chloramphenicol plates. We made separate liquid cultures of for two separate colonies per plate. After taking the OD600 of the overnight cultures, we diluted the cells in LB and chloramphenicol according to the volumes below in order to obtain a target absorbance of 0.02. </p>
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<a href="#"><img src="https://static.igem.org/mediawiki/2017/5/59/T--Stony_Brook--interlab3.png" style= "text-align: center;width:500px;height:500px;"/></a>
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Revision as of 03:52, 21 October 2017

Stony Brook 2017

This year, our team participated in the iGEM Fourth InterLab study. The objective of this study is to improve the measurement tools in order to obtain more reliable data within the field of synthetic biology. This year’s study aimed to improve the GFP and OD600 measurement protocol via the use of a plate reader. We were provided with 8 plasmids, including 2 controls and 6 test devices, to transform into DH5-alpha Escherichia Coli cells. We measured the fluorescence and absorbance with the Molecular Devices Microplate Reader SoftMax Pro 6.5.1.