(Prototype team page) |
|||
Line 1: | Line 1: | ||
− | + | <html xmlns="http://www.w3.org/1999/xhtml"> | |
− | < | + | <head> |
+ | <meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> | ||
+ | <title>Team:Nanjing-China - 2017.igem.org</title> | ||
+ | <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Nanjing-China/CSS/1?action=raw&ctype=text/css" /> | ||
+ | <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Nanjing-China/CSS/2?action=raw&ctype=text/css" /> | ||
+ | <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Nanjing-China/CSS/d3?action=raw&ctype=text/css" /> | ||
+ | <style type="text/css"> | ||
+ | .container .contain h1 { | ||
+ | font-family: "MS Serif", "New York", serif; | ||
+ | color: #006; | ||
+ | font-size: 250%; | ||
+ | } | ||
+ | </style> | ||
+ | <script type="text/javascript" src="https://2017.igem.org/Template:Nanjing-China/Javascript/1?action=raw&ctype=text/javascript"></script> | ||
+ | <script type="text/javascript" src="https://2017.igem.org/Template:Nanjing-China/Javascript/d3?action=raw&ctype=text/javascript"></script> | ||
+ | <script src="../../SpryAssets/SpryEffects.js" type="text/javascript"></script> | ||
+ | <script type="text/javascript"> | ||
+ | function menuFix(){ | ||
+ | var sfEles=document.getElementById("menu").getElementsByTagName("li"); | ||
+ | for(var i=0;i<sfEles.length; i++){ | ||
+ | sfEles[i].onmouseover=function(){ | ||
+ | this.className +=(this.className.length>0?"":"")+"listshow"; | ||
+ | } | ||
+ | sfEles[i].onmouseout=function(){ | ||
+ | this.className =this.className.replace("listshow",""); | ||
+ | } | ||
+ | } | ||
+ | } | ||
+ | window.onload= menuFix; | ||
+ | function MM_changeProp(objId,x,theProp,theValue) { //v9.0 | ||
+ | var obj = null; with (document){ if (getElementById) | ||
+ | obj = getElementById(objId); } | ||
+ | if (obj){ | ||
+ | if (theValue == true || theValue == false) | ||
+ | eval("obj.style."+theProp+"="+theValue); | ||
+ | else eval("obj.style."+theProp+"='"+theValue+"'"); | ||
+ | } | ||
+ | } | ||
− | + | function MM_effectAppearFade(targetElement, duration, from, to, toggle) | |
− | <div | + | { |
− | < | + | Spry.Effect.DoFade(targetElement, {duration: duration, from: from, to: to, toggle: toggle}); |
− | < | + | } |
− | < | + | function MM_effectBlind(targetElement, duration, from, to, toggle) |
+ | { | ||
+ | Spry.Effect.DoBlind(targetElement, {duration: duration, from: from, to: to, toggle: toggle}); | ||
+ | } | ||
+ | </script> | ||
+ | </head> | ||
+ | <body onload="MM_timelinePlay('Timeline2')"> | ||
+ | <div id="d"> | ||
+ | <div id="Layer1" style="visibility: hidden"><img src="https://static.igem.org/mediawiki/2017/8/87/T-Nanjing-China-chart-cell-1.png" width="180" /></div> | ||
+ | <div id="Layer2" style="visibility: hidden; left: 257px; top: 13px;"><img src="https://static.igem.org/mediawiki/2017/5/5d/T-Nanjing-China-h2-d-7.png" width="130" /></div> | ||
+ | <div id="Layer3" style="visibility: hidden; left: 273px; top: 189px;"><img src="https://static.igem.org/mediawiki/2017/d/d0/T-Nanjing-China-ch2o-d-8.png" width="120" /></div> | ||
+ | <div id="Layer4" style="visibility: hidden; left: 39px; top: 254px;"><img src="https://static.igem.org/mediawiki/2017/4/47/T-Nanjing-China-h2s-d-7.png" width="125" /></div> | ||
+ | <div id="Layer5" style="visibility: hidden"> | ||
+ | <div><img src="https://static.igem.org/mediawiki/2017/7/74/T-Nanjing-China-chart-cell.png" width="215" onclick="MM_effectBlind('HOME', 1000, '0%', '100%', false)" /></div> | ||
+ | <div align="center"> | ||
+ | <div id="HOME"> | ||
+ | <div class="sub"> | ||
+ | <ul><li><a href="https://2017.igem.org/Team:Nanjing-China/Collaborations">Collaberations</a></ul></li></div> | ||
</div> | </div> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
</div> | </div> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
</div> | </div> | ||
− | + | </div> | |
− | + | <div class="container" align="center"> | |
+ | <div class="header"><img src="https://static.igem.org/mediawiki/2017/e/e3/T-Nanjing-China-title.png" width="100%" onload="MM_effectAppearFade('menu', 4000, 0, 100, false)" /> | ||
+ | <div id="menu"> | ||
+ | <ul> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China">HOME</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China">Introduction</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/Background">Background</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/CH2O">PARTS</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/CH2O">CH<sub>2</sub>o</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/H2">H<sub>2</sub></a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/H2S">H<sub>2</sub>S</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/Sliver">JUDGE</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/Sliver">Sliver</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/Gold">Gold</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/Design">PROJECT</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/Design">Design</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/Notebook">Notebook</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/Demonstrate">Demonstrate</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/Improvement">Improvement</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/TEAM/Introduction">TEAM</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/TEAM/Introduction">Introduction</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/Members">Members</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/Attributions">Attributions</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/Collaborations">Collaboration</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/Safty">OTHERS</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/HP/Silver">Safety</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/Model">Model</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:Nanjing-China/HP/Gold_Integrated">HP</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="contain" onmousemove="MM_changeProp('d','','left','85%','DIV')"> | ||
+ | <div align="center"> | ||
+ | <h1>Collaboration</h1> | ||
+ | <div class="word"> | ||
+ | <div align="left"> | ||
+ | <p>We have formed online collaboration with NEFU since September. We informed each other about our progress and helped each other with experimental puzzles. Some of the problems we tackled together are listed as follows. </p> | ||
+ | <p><font color="#CC6600"><font size="+2">♝</font>:We plasmid vector can't be cut.</font></p> | ||
+ | <p><font color="#0099CC"><font size="+2">♗</font>:It may be because of the enzyme had losed potency :</font><br/> | ||
+ | <blockquote> | ||
+ | <font color="#0099CC">Plasmid was contaminated with protein</font> <br/> | ||
+ | <font color="#0099CC">The plasmid may be dissolved in a solution that inhibited the activity of the enzyme</font></p> | ||
+ | </blockquote> | ||
+ | <p><font color="#CC6600"><font size="+2">♝</font>:What should we do to link three parts efficiently?</font></p> | ||
+ | <p><font color="#0099CC"><font size="+2">♗</font>:Use isocaudamer.</font></p> | ||
+ | <p><font color="#CC6600"><font size="+2">♝</font>: What should we do when we we want to dye the sample by Comassie blue staining and WB.</font></p> | ||
+ | <p><font color="#0099CC"><font size="+2">♗</font>:When you add the samlpe, you should pay attention to the amount of it. Measure the value of OD to get the concentration of the sample you need and then add the sample. When making up polyacrylamide agarose gel, wash and dry the holder. In that way, you can get the gel free of bubble.</font></p> | ||
+ | </div></div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="footer"> | ||
+ | <div align="center"> | ||
+ | <div class="f"> | ||
+ | <div class="f-word"><p><strong><u>Address</u></strong></p> | ||
+ | <p>Life Science Department<br /> | ||
+ | #163 Xianlin Blvd, Qixia District<br /> | ||
+ | Nanjing University<br /> | ||
+ | Nanjing, Jiangsu Province<br /> | ||
+ | P.R. of China<br /> | ||
+ | Zip: 210046 | ||
+ | </p> | ||
+ | <p><strong><u>Email</u></strong></p> | ||
+ | <p>nanjing_china@163.com</p> | ||
+ | <p><strong><u>Social media</u></strong></p> | ||
+ | <p>Twitter button: <br/> | ||
+ | <a href="https://twitter.com/Guja1501">iGEM Nanjing-China@nanjing_china</a></p> | ||
+ | </div> | ||
+ | <div class="f-img"> | ||
+ | <div align="center"><img src="https://static.igem.org/mediawiki/2017/0/04/T-Nanjing-China-%E5%8D%97%E4%BA%AC%E5%A4%A7%E5%AD%A6.png" name="img1" width="160" height="220" id="img1" onmouseover="MM_changeProp('img1','','width','180px','IMG');MM_changeProp('img1','','height','240px','IMG')" onmouseout="MM_changeProp('img1','','width','160px','IMG');MM_changeProp('img1','','height','220px','IMG')" /></div> | ||
+ | </div> | ||
+ | <div class="f-img"> | ||
+ | <div align="right"><img src=" https://static.igem.org/mediawiki/2017/d/d4/T-Nanjing-China-%E5%9B%BE%E7%89%873.png | ||
+ | " name="img2" width="160" height="220" id="img2" onmouseover="MM_changeProp('img2','','width','180px','IMG');MM_changeProp('img2','','height','228px','IMG')" onmouseout="MM_changeProp('img2','','width','140px','IMG');MM_changeProp('img2','','height','220px','IMG')" /></div> | ||
+ | </div> | ||
+ | <div class="f-img"> | ||
+ | <div align="center"><img src="https://static.igem.org/mediawiki/2017/3/37/T-Nanjing-China-s1.png" name="img3" width="160" height="160" id="img3" onmouseover="MM_changeProp('img3','','width','180px','IMG');MM_changeProp('img3','','height','180px','IMG')" onmouseout="MM_changeProp('img3','','width','160px','IMG');MM_changeProp('img3','','height','160px','IMG')" align="absmiddle" /></div> | ||
+ | </div> | ||
+ | <div class="f-img"> | ||
+ | <div align="right"><img src="https://static.igem.org/mediawiki/2017/d/dc/T-Nanjing-China-%E5%9B%BE%E7%89%872.png" name="img4" width="160" height="160" id="img4" onmouseover="MM_changeProp('img4','','width','180px','IMG');MM_changeProp('img4','','height','180px','IMG')" onmouseout="MM_changeProp('img4','','width','160px','IMG');MM_changeProp('img4','','height','160px','IMG')" /></div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/9/91/T-Nanjing-China-foot.png" width="100%"/> | ||
+ | </div> | ||
+ | </div> | ||
+ | </body> | ||
</html> | </html> |
Revision as of 11:56, 24 October 2017
Collaboration
We have formed online collaboration with NEFU since September. We informed each other about our progress and helped each other with experimental puzzles. Some of the problems we tackled together are listed as follows.
♝:We plasmid vector can't be cut.
♗:It may be because of the enzyme had losed potency :
Plasmid was contaminated with protein
The plasmid may be dissolved in a solution that inhibited the activity of the enzyme
♝:What should we do to link three parts efficiently?
♗:Use isocaudamer.
♝: What should we do when we we want to dye the sample by Comassie blue staining and WB.
♗:When you add the samlpe, you should pay attention to the amount of it. Measure the value of OD to get the concentration of the sample you need and then add the sample. When making up polyacrylamide agarose gel, wash and dry the holder. In that way, you can get the gel free of bubble.