Difference between revisions of "Team:IISc-Bangalore/Design"

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{{IISc-Bangalore}}
 
{{IISc-Bangalore}}
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<html>
 
  
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<div id="inPageNav">
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            <ol>
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                <li>T7 promoter</li>
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                <li>T7 terminator</li>
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                <li>sfGFP</li>
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                <li>SpyCatcher</li>
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                <li>mCherry</li>
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                <li>T7 expression system</li>
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                <li>sfGFP-SpyCatcher fusion</li>
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                <li>mCherry-SpyTag fusion</li>
  
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            </ol>
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<div class="column full_size">
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<h1>BioBricks designed</h1>
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<p>
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Design is the first step in the design-build-test cycle in engineering and synthetic biology. Use this page to describe the process that you used in the design of your parts. You should clearly explain the engineering principles used to design your project.
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</p>
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<h2>sfGFP tagged to gvpC C-terminally</h2>
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<h2>sfGFP tagged to gvpC N-terminally</h2>
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<h2>sfGFP tagged to gvpC internally</h2>
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<h2>iFLOAT adaptor</h2>
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<h2>Secretory gvpA for <i>Pichia pastoris</i></h2>
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<h2>Secretory gvpC-tagged sfGFP for <i>Pichia pastoris</i></h2>
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<p>
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This page is different to the "Applied Design Award" page. Please see the <a href="https://2017.igem.org/Team:IISc-Bangalore/Applied_Design">Applied Design</a> page for more information on how to compete for that award.
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</p>
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</div>
 
</div>
  
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<div class="column half_size">
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<div id="contentMain">
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<h5>What should this page contain?</h5>
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<ul>
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<li>Explanation of the engineering principles your team used in your design</li>
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<li>Discussion of the design iterations your team went through</li>
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<li>Experimental plan to test your designs</li>
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</ul>
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<h1>T7 promoter (BBa_K525998)</h1>
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<h1>T7 terminator (BBa_K731721)</h1>
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<h1>sfGFP (BBa_K1321337)</h1>
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<h1>SpyCatcher (BBa_K1650037)</h1>
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<h1>mCherry (BBa_J18932)</h1>
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<h1>Building a modular T7 expression system</h1>
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<h1>sfGFP-SpyCatcher fusion protein</h1>
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<h1>6xHis-mCherry-SpyTag fusion protein</h1>
 
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</div>
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<div class="column half_size">
 
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<h5>Inspiration</h5>
 
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<ul>
 
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<li><a href="https://2016.igem.org/Team:MIT/Experiments/Promoters">2016 MIT</a></li>
 
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<li><a href="https://2016.igem.org/Team:BostonU/Proof">2016 BostonU</a></li>
 
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<li><a href="https://2016.igem.org/Team:NCTU_Formosa/Design">2016 NCTU Formosa</a></li>
 
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</ul>
 
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</div>
 
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</html>
 

Revision as of 17:54, 24 October 2017

  1. T7 promoter
  2. T7 terminator
  3. sfGFP
  4. SpyCatcher
  5. mCherry
  6. T7 expression system
  7. sfGFP-SpyCatcher fusion
  8. mCherry-SpyTag fusion

T7 promoter (BBa_K525998)

T7 terminator (BBa_K731721)

sfGFP (BBa_K1321337)

SpyCatcher (BBa_K1650037)

mCherry (BBa_J18932)

Building a modular T7 expression system

sfGFP-SpyCatcher fusion protein

6xHis-mCherry-SpyTag fusion protein