Difference between revisions of "Team:Gaston Day School/Notebook"

Line 262: Line 262:
 
   <tbody>
 
   <tbody>
 
     <tr>
 
     <tr>
−
       <td></td>
+
       <td>gDNA</td>
−
       <td></td>
+
       <td>3</td>
−
       <td></td>
+
       <td>24</td>
 +
    </tr>
 +
    <tr>
 +
      <td>Buffer</td>
 +
      <td>2.5</td>
 +
      <td>20</td>
 +
    </tr>
 +
    <tr>
 +
      <td>dNTP</td>
 +
      <td>1</td>
 +
      <td>8</td>
 +
    </tr>
 +
    <tr>
 +
      <td>dH2O</td>
 +
      <td>12.3</td>
 +
      <td>98.4</td>
 +
    </tr>
 +
    <tr>
 +
      <td>taq</td>
 +
      <td>0.2</td>
 +
      <td>1.6</td>
 +
    </tr>
 +
    <tr>
 +
      <td>MgCl2</td>
 +
      <td>1</td>
 +
      <td>8</td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
<table>
 +
  </tbody>
 +
    <tr>
 +
      <td>/</td>
 +
      <td>dH2O</td>
 +
      <td>For</td>
 +
      <td>Rev</td>
 +
      <td>/</td>
 +
    </tr>
 +
    <tr>
 +
      <td>0</td>
 +
      <td>5</td>
 +
      <td>-</td>
 +
      <td>-</td>
 +
      <td>-</td>
 +
    </tr>
 +
    <tr>
 +
      <td>1</td>
 +
      <td>4</td>
 +
      <td>0.5</td>
 +
      <td>0.5</td>
 +
      <td>ACE F</td>
 +
    </tr>
 +
    <tr>
 +
      <td>2</td>
 +
      <td>4</td>
 +
      <td>0.5</td>
 +
      <td>0.5</td>
 +
      <td>EutG</td>
 +
    </tr>
 +
    <tr>
 +
      <td>3</td>
 +
      <td>4</td>
 +
      <td>0.5</td>
 +
      <td>0.5</td>
 +
      <td>YiaY</td>
 +
    </tr>
 +
    <tr>
 +
      <td>4</td>
 +
      <td>4</td>
 +
      <td>0.5</td>
 +
      <td>0.5</td>
 +
      <td>AdhP</td>
 +
    </tr>
 +
    <tr>
 +
      <td>5</td>
 +
      <td>4</td>
 +
      <td>0.5</td>
 +
      <td>0.5</td>
 +
      <td>Lpd</td>
 +
    </tr>
 +
    <tr>
 +
      <td>6</td>
 +
      <td>4</td>
 +
      <td>0.5</td>
 +
      <td>0.5</td>
 +
      <td>AceE</td>
 
     </tr>
 
     </tr>
 
   </tbody>
 
   </tbody>

Revision as of 19:31, 24 October 2017

Notebook

On this page, Gaston Day School iGEM team would like to share with you our notes during the experiments from 09/01/2017 to 10/23/2017. The team leader Heena Saqib records the data patiently.

MeCl:

??? ??? ???
14.3 H2O 114.4
2.5 Buffer 20
1 MgCl2 8
1 dNTP 8

tube:

2ul H2O
-5ul F
-5ul R

Lable:

0 Control
1 AceF
2 EutG BB
3 YiaY BB
4 Adhp BB
5 Lpol
6 AceE

Maeten Mix

gDNA 3 24
Buffer 2.5 20
??? 1 8
dH2O 13.3 106.4
??? 0.2 1.6
/ dH2O For Rev /
1 4 0.5 0.5 AceF
2 4 0.5 0.5 EutG
3 4 0.5 0.5 YiaY
4 4 0.5 0.5 AdnP
5 4 0.5 0.5 Lpd
6 4 0.5 0.5 AceE
7 5 - - -

Maeten Mix

gDNA 3 24
Buffer 2.5 20
dNTP 1 8
dH2O 12.3 98.4
taq 0.2 1.6
MgCl2 1 8
/ dH2O For Rev /
0 5 - - -
1 4 0.5 0.5 ACE F
2 4 0.5 0.5 EutG
3 4 0.5 0.5 YiaY
4 4 0.5 0.5 AdhP
5 4 0.5 0.5 Lpd
6 4 0.5 0.5 AceE

 

Email: iGEM@gastonday.org

Gaston Day School

2001 Gaston Day School Rd

Gastonia 28056