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Revision as of 19:34, 24 October 2017
Notebook
On this page, Gaston Day School iGEM team would like to share with you our notes during the experiments from 09/01/2017 to 10/23/2017. The team leader Heena Saqib records the data patiently.
MeCl:
??? | ??? | ??? |
---|---|---|
14.3 | H2O | 114.4 |
2.5 | Buffer | 20 |
1 | MgCl2 | 8 |
1 | dNTP | 8 |
tube:
2ul | H2O |
-5ul | F |
-5ul | R |
Lable:
0 | Control |
1 | AceF |
2 | EutG BB |
3 | YiaY BB |
4 | Adhp BB |
5 | Lpol |
6 | AceE |
Maeten Mix
gDNA | 3 | 24 |
Buffer | 2.5 | 20 |
??? | 1 | 8 |
dH2O | 13.3 | 106.4 |
??? | 0.2 | 1.6 |
/ | dH2O | For | Rev | / |
---|---|---|---|---|
1 | 4 | 0.5 | 0.5 | AceF |
2 | 4 | 0.5 | 0.5 | EutG |
3 | 4 | 0.5 | 0.5 | YiaY |
4 | 4 | 0.5 | 0.5 | AdnP |
5 | 4 | 0.5 | 0.5 | Lpd |
6 | 4 | 0.5 | 0.5 | AceE |
7 | 5 | - | - | - |
Maeten Mix
gDNA | 3 | 24 |
Buffer | 2.5 | 20 |
dNTP | 1 | 8 |
dH2O | 12.3 | 98.4 |
taq | 0.2 | 1.6 |
MgCl2 | 1 | 8 |
/ | dH2O | For | Rev | / |
---|---|---|---|---|
0 | 5 | - | - | - |
1 | 4 | 0.5 | 0.5 | ACE F |
2 | 4 | 0.5 | 0.5 | EutG |
3 | 4 | 0.5 | 0.5 | YiaY |
4 | 4 | 0.5 | 0.5 | AdhP |
5 | 4 | 0.5 | 0.5 | Lpd |
6 | 4 | 0.5 | 0.5 | AceE |