Difference between revisions of "Team:Gaston Day School/Notebook"

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<div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
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            <div class="panel panel-default">
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                <div class="panel-heading" role="tab" id="P4">
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                    <h4 class="panel-title">
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                    <div class="col-md-11">09/18/2017 PCR check of J23102 and GlmY</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div>
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<p>Maeten Mix</p>
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<table class="table table-bordered table-striped">
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  <thead>
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    <th>
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    <th>1</th>
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    <th>-</th>
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    <th>DNA(0)</th>
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    <th>dH2O(5)</th>
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    </tr>
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  </thead>
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  <tbody>
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    <tr>
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      <td>2</td>
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      <td>J23102</td>
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      <td>3</td>
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      <td>2</td>
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    </tr>
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    <tr>
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      <td>3</td>
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      <td>GlmY</td>
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      <td>3</td>
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      <td>2</td>
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    <tr>
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      <td>4</td>
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      <td>A(1)</td>
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      <td>3</td>
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      <td>2</td>
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    </tr>
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    <tr>
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      <td>5/td>
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      <td>A(2)</td>
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      <td>3</td>
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      <td>2</td>
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    </tr>
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    <tr>
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      <td>6</td>
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      <td>B(2)</td>
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      <td>3</td>
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      <td>2</td>
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    </tr>
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    <tr>
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      <td>7</td>
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      <td>C(2)</td>
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      <td>3</td>
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      <td>2</td>
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    </tr>
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  </tbody>
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</table>
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<p>Max</p>
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<table class="table table-bordered table-striped">
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  <tbody>
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    <tr>
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      <td>Buffer</td>
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      <td>2.5</td>
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      <td>20</td>
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    </tr>
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    <tr>
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      <td>dNTP</td>
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      <td>1</td>
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      <td>8</td>
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    </tr>
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    <tr>
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      <td>VR</td>
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      <td>0.5</td>
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      <td>4</td>
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    </tr>
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    <tr>
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      <td>VF2</td>
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      <td>0.5</td>
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      <td>4</td>
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    </tr>
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    <tr>
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      <td>taq</td>
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      <td>0.2</td>
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      <td>1.6</td>
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    </tr>
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    <tr>
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      <td>dH2O</td>
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      <td>15.3</td>
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      <td>122.4</td>
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    </tr>
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  </tbody>
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</table>
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</div>
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Revision as of 19:49, 24 October 2017

Notebook

On this page, Gaston Day School iGEM team would like to share with you our notes during the experiments from 09/01/2017 to 10/23/2017. The team leader Heena Saqib records the data patiently.

MeCl:

??? ??? ???
14.3 H2O 114.4
2.5 Buffer 20
1 MgCl2 8
1 dNTP 8

tube:

2ul H2O
-5ul F
-5ul R

Lable:

0 Control
1 AceF
2 EutG BB
3 YiaY BB
4 Adhp BB
5 Lpol
6 AceE

Maeten Mix

gDNA 3 24
Buffer 2.5 20
??? 1 8
dH2O 13.3 106.4
??? 0.2 1.6
/ dH2O For Rev /
1 4 0.5 0.5 AceF
2 4 0.5 0.5 EutG
3 4 0.5 0.5 YiaY
4 4 0.5 0.5 AdnP
5 4 0.5 0.5 Lpd
6 4 0.5 0.5 AceE
7 5 - - -

Maeten Mix

gDNA 3 24
Buffer 2.5 20
dNTP 1 8
dH2O 12.3 98.4
taq 0.2 1.6
MgCl2 1 8
/ dH2O For Rev /
0 5 - - -
1 4 0.5 0.5 ACE F
2 4 0.5 0.5 EutG
3 4 0.5 0.5 YiaY
4 4 0.5 0.5 AdhP
5 4 0.5 0.5 Lpd
6 4 0.5 0.5 AceE

Maeten Mix

1 - DNA(0) dH2O(5)
2 J23102 3 2
3 GlmY 3 2
4 A(1) 3 2
5/td> A(2) 3 2
6 B(2) 3 2
7 C(2) 3 2

Max

Buffer 2.5 20
dNTP 1 8
VR 0.5 4
VF2 0.5 4
taq 0.2 1.6
dH2O 15.3 122.4

 

Email: iGEM@gastonday.org

Gaston Day School

2001 Gaston Day School Rd

Gastonia 28056