Difference between revisions of "Team:Gaston Day School/Notebook"

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                     <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P3-collapse" aria-expanded="false" aria-controls="P3-collapse">
 
<div>
 
<div>
−
                     <div class="col-md-11">09/06/2017 Geneyuic PCR</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div>
+
                     <div class="col-md-11">09/25/2017 PCR check for J23102+GlmY</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div>
 
</div>
 
</div>
 
                     </a>
 
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                     <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P5-collapse" aria-expanded="false" aria-controls="P5-collapse">
 
<div>
 
<div>
−
                     <div class="col-md-11">09/06/2017 Geneyuic PCR</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div>
+
                     <div class="col-md-11">10/10/2017 Geneyuic PCR</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div>
 
</div>
 
</div>
 
                     </a>
 
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   <tbody>
 
   <tbody>
 
     <tr>
 
     <tr>
−
       <td></td>
+
       <td>Buffer</td>
−
       <td></td>
+
       <td>2.5</td>
−
       <td></td>
+
       <td>20</td>
 +
    </tr>
 +
    <tr>
 +
      <td>dNTP</td>
 +
      <td>1</td>
 +
      <td>8</td>
 +
    </tr>
 +
    <tr>
 +
      <td>VR</td>
 +
      <td>0.5</td>
 +
      <td>4</td>
 +
    </tr>
 +
    <tr>
 +
      <td>VF2</td>
 +
      <td>0.5</td>
 +
      <td>4</td>
 +
    </tr>
 +
    <tr>
 +
      <td>taq</td>
 +
      <td>0.2</td>
 +
      <td>1.6</td>
 +
    </tr>
 +
    <tr>
 +
      <td>dH2O</td>
 +
      <td>15.3</td>
 +
      <td>122.4</td>
 
     </tr>
 
     </tr>
 
   </tbody>
 
   </tbody>
 
</table>
 
</table>
 +
<table class="table table-bordered table-striped">
 +
  <thead>
 +
    <th></th>
 +
    <th></th>
 +
    <th>DNA</th>
 +
    <th>H2O</th>
 +
    </tr>
 +
  </thead>
 +
  <tbody>
 +
    <tr>
 +
      <td>1</td>
 +
      <td>Control</td>
 +
      <td>0u</td>
 +
      <td>5u</td>
 +
    </tr>
 +
    <tr>
 +
      <td>2</td>
 +
      <td>J231013</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>3</td>
 +
      <td>GlmY-3A</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>4</td>
 +
      <td>2C</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>5</td>
 +
      <td>1C</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>6</td>
 +
      <td>1B</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>7</td>
 +
      <td>1A</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
</div>
 +
</div>
 +
</div>
 +
 +
 +
<div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
 +
            <div class="panel panel-default">
 +
                <div class="panel-heading" role="tab" id="P6">
 +
                    <h4 class="panel-title">
 +
                    <a role="button" data-toggle="collapse" data-parent="#accordion" href="#P6-collapse" aria-expanded="false" aria-controls="P6-collapse">
 +
<div>
 +
                    <div class="col-md-11">10/20/2017 PCR J23102+GlmY</div><div class="col-md-1"><i class="fa fa-arrow-down fa-10" aria-hidden="true"></i></div>
 +
</div>
 +
                    </a>
 +
                    </h4>
 +
 +
                </div>
 +
                <div id="P6-collapse" class="panel-collapse collapse" role="tabpanel" aria-labelledby="P6">
 +
                    <div class="panel-body">
 +
<p>Maeten Mix</p>
 +
<p>Maeten Mix</p>
 +
<table class="table table-bordered table-striped">
 +
  <tbody>
 +
    <tr>
 +
      <td>Buffer</td>
 +
      <td>2.5</td>
 +
      <td>20</td>
 +
    </tr>
 +
    <tr>
 +
      <td>dNTP</td>
 +
      <td>1</td>
 +
      <td>8</td>
 +
    </tr>
 +
    <tr>
 +
      <td>VR</td>
 +
      <td>0.5</td>
 +
      <td>4</td>
 +
    </tr>
 +
    <tr>
 +
      <td>VF2</td>
 +
      <td>0.5</td>
 +
      <td>4</td>
 +
    </tr>
 +
    <tr>
 +
      <td>taq</td>
 +
      <td>0.2</td>
 +
      <td>1.6</td>
 +
    </tr>
 +
    <tr>
 +
      <td>dH2O</td>
 +
      <td>15.3</td>
 +
      <td>122.4</td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
<table class="table table-bordered table-striped">
 +
  <thead>
 +
    <th></th>
 +
    <th></th>
 +
    <th>DNA</th>
 +
    <th>H2O</th>
 +
    </tr>
 +
  </thead>
 +
  <tbody>
 +
    <tr>
 +
      <td>1</td>
 +
      <td>Control</td>
 +
      <td>0u</td>
 +
      <td>5u</td>
 +
    </tr>
 +
    <tr>
 +
      <td>2</td>
 +
      <td>J23102B pp</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>3</td>
 +
      <td>J23102 P NNP</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>4</td>
 +
      <td>GlmY-3A</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>5</td>
 +
      <td>102B+GlmY 1B</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>6</td>
 +
      <td>102B+GlmY 1C</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
    <tr>
 +
      <td>7</td>
 +
      <td>102B_GlmY 1D</td>
 +
      <td>3</td>
 +
      <td>2</td>
 +
    </tr>
 +
  </tbody>
 +
</table>
 +
 
</div>
 
</div>
 
</div>
 
</div>

Revision as of 20:05, 24 October 2017

Notebook

On this page, Gaston Day School iGEM team would like to share with you our notes during the experiments from 09/01/2017 to 10/23/2017. The team leader Heena Saqib records the data patiently.

MeCl:

??? ??? ???
14.3 H2O 114.4
2.5 Buffer 20
1 MgCl2 8
1 dNTP 8

tube:

2ul H2O
-5ul F
-5ul R

Lable:

0 Control
1 AceF
2 EutG BB
3 YiaY BB
4 Adhp BB
5 Lpol
6 AceE

Maeten Mix

gDNA 3 24
Buffer 2.5 20
??? 1 8
dH2O 13.3 106.4
??? 0.2 1.6
/ dH2O For Rev /
1 4 0.5 0.5 AceF
2 4 0.5 0.5 EutG
3 4 0.5 0.5 YiaY
4 4 0.5 0.5 AdnP
5 4 0.5 0.5 Lpd
6 4 0.5 0.5 AceE
7 5 - - -

Maeten Mix

gDNA 3 24
Buffer 2.5 20
dNTP 1 8
dH2O 12.3 98.4
taq 0.2 1.6
MgCl2 1 8
/ dH2O For Rev /
0 5 - - -
1 4 0.5 0.5 ACE F
2 4 0.5 0.5 EutG
3 4 0.5 0.5 YiaY
4 4 0.5 0.5 AdhP
5 4 0.5 0.5 Lpd
6 4 0.5 0.5 AceE
1 - DNA(0) dH2O(5)
2 J23102 3 2
3 GlmY 3 2
4 A(1) 3 2
5/td> A(2) 3 2
6 B(2) 3 2
7 C(2) 3 2

Max

Buffer 2.5 20
dNTP 1 8
VR 0.5 4
VF2 0.5 4
taq 0.2 1.6
dH2O 15.3 122.4

Maeten Mix

Buffer 2.5 20
dNTP 1 8
VR 0.5 4
VF2 0.5 4
taq 0.2 1.6
dH2O 15.3 122.4
DNA H2O
1 Control 0u 5u
2 J231013 3 2
3 GlmY-3A 3 2
4 2C 3 2
5 1C 3 2
6 1B 3 2
7 1A 3 2

Maeten Mix

Buffer 2.5 20
dNTP 1 8
VR 0.5 4
VF2 0.5 4
taq 0.2 1.6
dH2O 15.3 122.4
DNA H2O
1 Control 0u 5u
2 J231013 3 2
3 GlmY-3A 3 2
4 2C 3 2
5 1C 3 2
6 1B 3 2
7 1A 3 2

Maeten Mix

Maeten Mix

Buffer 2.5 20
dNTP 1 8
VR 0.5 4
VF2 0.5 4
taq 0.2 1.6
dH2O 15.3 122.4
DNA H2O
1 Control 0u 5u
2 J23102B pp 3 2
3 J23102 P NNP 3 2
4 GlmY-3A 3 2
5 102B+GlmY 1B 3 2
6 102B+GlmY 1C 3 2
7 102B_GlmY 1D 3 2

 

Email: iGEM@gastonday.org

Gaston Day School

2001 Gaston Day School Rd

Gastonia 28056