Difference between revisions of "Team:Gaston Day School/Experiment"

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   <div>
 
   <div>
 
   <h4>Labels</h4>
 
   <h4>Labels</h4>
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   <p style="font-weight: bolder;">For MCF tubes</p>
+
   <p style="font-weight: bolder;">For MCF tubes:</p>
 +
  <ol>
 +
    <li>P: Plasmid Prep</li>
 +
    <li>D: Digest</li>
 +
    <li>T: Transformation</li>
 +
    <li>L: Ligation</li>
 +
  </ol>
 
   </div>
 
   </div>
  
−
 
+
  <div>
 +
  <h4>Transformation</h4>
 +
  <ol>
 +
    <li>x = the number of transformations you are doing</li>
 +
    <li>Place the SOC medium (from the fridge) in the incubator</li>
 +
    <li>Also place X plates in the incubator (With the appropriate antibiotic resistance, Chl for complete, Amp for parts)</li>
 +
    <li>Make an ice-water bath</li>
 +
    <li>Obtain X MCF tubes and a float</li>
 +
    <li>Add 50 ul of competent cells</li>
 +
    <li>Add 2ul of the DNA</li>
 +
    <li>Place it in the ice-water bath for 2 mins</li>
 +
    <li>While waiting, turn on the sink's hot water, obtain a beaker, add hot water, and using a thermometer, adjust water to 42o C (using the hot plate if needed)</li>
 +
    <li>Place tubes in the incubator for 30 seconds</li>
 +
    <li>Another 2 mins on ice</li>
 +
    <li>Add 1 ml of warm SOC medium</li>
 +
    <li>Place each tube in the incubator (37o C) for 1 hour</li>
 +
    <li>Plating</li>
 +
  </ol>
 +
  </div>
  
  

Revision as of 00:06, 25 October 2017

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Experiments and Protocol

Labels

For MCF tubes:

  1. P: Plasmid Prep
  2. D: Digest
  3. T: Transformation
  4. L: Ligation

Transformation

  1. x = the number of transformations you are doing
  2. Place the SOC medium (from the fridge) in the incubator
  3. Also place X plates in the incubator (With the appropriate antibiotic resistance, Chl for complete, Amp for parts)
  4. Make an ice-water bath
  5. Obtain X MCF tubes and a float
  6. Add 50 ul of competent cells
  7. Add 2ul of the DNA
  8. Place it in the ice-water bath for 2 mins
  9. While waiting, turn on the sink's hot water, obtain a beaker, add hot water, and using a thermometer, adjust water to 42o C (using the hot plate if needed)
  10. Place tubes in the incubator for 30 seconds
  11. Another 2 mins on ice
  12. Add 1 ml of warm SOC medium
  13. Place each tube in the incubator (37o C) for 1 hour
  14. Plating