Difference between revisions of "Team:RPI Troy NY/Description"

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Revision as of 01:43, 25 October 2017

Home Team Collaborations Project Results
Interlab Study Parts Safety Human Practices Attributions

Project Description

♦Background♦

♦Design♦

In this project, we sought to increase the production of sophorolipids by taking a two-part approach. In the first part, we engineered primers and inserted these into the yeast to be able to produce either normal amounts of sophorolipids or lesser amounts of sophorolipids (gene knockout). In the second part, we extracted these sophorolipids and completed analysis on this.

Protocols

♦Microbial Genetics Protocols♦

Agarose Gels

We grew the yeast in two types of broth: FM2 and PM2. This is the components of each of those broths.
PM2 Ingredients Amt.(g/L) FM2 Ingredients Amt.(g/L)
Glucose 200 Glucose 100
Yeast Extract 5 Yeast Extract 5
KH2PO4 1 KH2PO4 1
MgSO4 0.5 MgSO4 0.5
CaCl2 0.1 CaCl2 0.1
NaCl 0.1 NaCl 0.1
Peptone 0.7 Peptone 0.7

Experiments

Notebook