Difference between revisions of "Team:RPI Troy NY/Description"
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<h2 style="align:center" <font face = "Palatino"><font size = "4"> <b> ♦Microbial Genetics Protocols♦</b></h2> | <h2 style="align:center" <font face = "Palatino"><font size = "4"> <b> ♦Microbial Genetics Protocols♦</b></h2> | ||
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<strong><h3 style="align:left" <font face = "Palatino"><font size = "3"> <b> Agarose Gels</b></h3><strong> | <strong><h3 style="align:left" <font face = "Palatino"><font size = "3"> <b> Agarose Gels</b></h3><strong> | ||
<p style="padding-left:10px"><font face= "Times New Roman"> <font size ="3">Analytical Gel</p> | <p style="padding-left:10px"><font face= "Times New Roman"> <font size ="3">Analytical Gel</p> | ||
Revision as of 02:05, 25 October 2017
| Home | Team | Collaborations | Project | Results |
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Project Description
♦Background♦♦Design♦In this project, we sought to increase the production of sophorolipids by taking a two-part approach. In the first part, we engineered primers and inserted these into the yeast to be able to produce either normal amounts of sophorolipids or lesser amounts of sophorolipids (gene knockout). In the second part, we extracted these sophorolipids and completed analysis on this. 
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Protocols
♦Microbial Genetics Protocols♦Agarose GelsAnalytical Gel • 1-2% Agarose • Ethidium Bromide (1uL/100mL) Purification Gel • .8% Agarose • Ethidium Bromide (1uL/100mL) PCR Amplification• 50ng Template DNA • 25uL Accuzyme or Hotstart Mix (1uL/100mL) • 1uL pure DMSO (Optional, use if high GC content) • 1uL primer stock (10mmol forward and reverse primer) • Remainder MilliQ 50uL total
ExperimentsNotebook
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