Thursday, June 1st.

Friday, June 2nd.

Saturday, June 3rd.

Sunday, June 4th.

Monday, June 5th.

Tuesday, June 6th.

Wednesday, June 7th.

Thursday, June 8th.

Friday, June 9th.

Saturday, June 10th.

Sunday, June 11th.

Monday, June 12th.

First day of induction week!!!!!

(thanks Marc and Will for helping us!)

Tuesday, June 13th.

Second day of induction week!!!!

(thanks Marc and Will for helping us!)

Wednesday, June 14th.

Third day of induction week!!!

(thanks Marc and Will for helping us!)

Thursday, June 15th.

Forth day of induction week!!

(thanks Marc and Will for helping us!)

First full team photo taken in the lab ⬎

Friday, June 16th.

Fifth and final day of induction week!

(thanks Marc and Will for helping us!)

We also upgraded our office door ⬎

Saturday, June 17th.

Weekend meet-up - MORE PLANNING!

Sunday, June 18th.

Monday, June 19th.

Planning...

Tuesday, June 20th.

Meeting with Rainer's research group (Maria and Alia) to discuss modelling the phosphate starvation operon.

Wednesday, June 21st.

More planning...

'To understand the literature, you must first become the literature...'

Thursday, June 22nd.

MathWorks (MatLab) free trial starts - we can start modelling our project!

Friday, June 23rd.

Skype Meeting with Markus Gershater from Synthace to learn about Design of Experiment (DoE) and how it could be applied to our experiment.

Snapgene free trial starts!

Saturday, June 24th.

Sunday, June 25th.

Monday, June 26th.

James, Adam, and Amber attended an iGEM meet-up in Edinburgh

(Thanks to the Edinburgh OG team for the great picture!)

Tuesday, June 27th.

Project presentation to organization representatives Claire Doherty (IBCarb) and Marc Corbett (BioCatNet).

Wednesday, June 28th.

EUSynBioS publish an article about our project!

You can read it here... http://www.eusynbios.org/blog/igem2017mcr/

Thursday, June 29th.

Even more planning...

Friday, June 30th.

PLANNING PLANNING PLANNING (going crazy in the office)

Saturday, July 1st.

Meet up in Jess's place to discuss project plan including DoE factors.

Sunday, July 2nd.

Meet up in Learning Commons to make project plan more presentable. Amber got into AGLC for the first time.

Important meeting with advisors and supervisors tomorrow.

Monday, July 3rd.

Meeting is canceled. More time to brush up project plan!

Tuesday, July 4th.

More time to brush up project plan! Important meeting tomorrow.

Wednesday, July 5th.

Meeting with Dr Duncan Thomas and discussed the viability of applying our project in the water industry.

Very important meeting with advisors and supervisors. Finally the whole project is set on trail and we are ready to roll!

Thursday, July 6th.

Alice is back from Canada!

DIARY entry page is created! =D

G BLock sequences are checked and double-checked before being sent to IDT for free synthesis.

Friday, July 7th.

G BLock sequences are checked and double-checked again and (finally!) sent to IDT for free synthesis. Word is that the orders will be delivered on 18th and 20th July.

Saturday, July 8th.

Sunday, July 9th.

Monday, July 10th.

Interlab study: Protocol was started by Alice and Theo! Inoculating competent cells.

Tuesday, July 11th.

Interlab study: Making not-yet-so-competent cells competent.

Wednesday, July 12th.

Interlab study: Competent cells are extraordinarily competent!

11.00 am GMT: Skype call with Peshawar iGEM 2017! Visit their website: https://2017.igem.org/Team:Peshawar

Thursday, July 13th.

Thanks to IDT our PPK gBlocks arrived today! 5 days earlier than expected!

Friday, July 14th.

Thanks to IDT our EutMN gBlocks arrived today!

Saturday, July 15th.

Sunday, July 16th.

Monday, July 17th.

Restriction digest of PPK in pNIC proved that transformation worked! Sequencing is in order.

Skype call with an iGEM alumni - Ben Reeves from CustoMem.

Tuesday, July 18th.

More PPK stuff.

Meeting with supervisors for update on everything we have been doing.

Wednesday, July 19th.

Interview with Jonathan Abra - Innovation UK

EutS and EutMN Gblocks arrive from IDT, and the eut lab work starts!

Thursday, July 20th.

Cloning EutS + EutMN - restriction digest, ligate, transform

Skype call with Virginia iGEM about potential collaborations

Friday, July 21st.

We have colonies! Put plates in fridge over weekend, ready to inoculate on Monday and miniprep on Tuesday.

Saturday, July 22nd.

Sunday, July 23rd.

Monday, July 24th.

Tuesday, July 25th.

Conference Call with Department of Environment, Food and Rural Affairs (DEFRA)

Checking EutS + EutMN transformation - miniprep, restriction digest and run agarose gel to confirm size

Wednesday, July 26th.

Results from yesterday = all negative; but sceptical as we have lots of colonies.. try colony PCR of the plates.

Thursday, July 27th.

Eut colony PCR (using primers VR + VF2) results = several successful EutS colonies, none for EutMN. Redo digest, ligation and transformation of EutMN. Miniprep EutS and restriction digest to confirm.

Meeting up with Warwick iGEM team during International Biology Olympiad in Coventry. Visit them at https://2017.igem.org/Team:Warwick

Friday, July 28th.

EutLK Gblock arrives from IDT: get going with cloning - restriction digest, ligation, transformation

Humanity Hallows publish an article about our project!

Saturday, July 29th.

Phone Call with Dr. Linda Kahl - BioBricks Foundation

Sunday, July 30th.

Monday, July 31st.

We have colonies on the EutMN plate - inoculate!

EutS: miniprep those showing positive by colony PCR - restriction digest and run gel to confirm size. Results = several produce expected results - can now send for sequencing.

Tuesday, August 1st.

EutMN miniprep, restriction digest and agarose gel = expected result.. now can send for sequencing!

Wednesday, August 2nd.

Thursday, August 3rd.

Friday, August 4th.

Saturday, August 5th.

Sunday, August 6th.

Monday, August 7th.

Tuesday, August 8th

Meeting with United Utilities. https://www.unitedutilities.com/

Wednesday, August 9th

Thursday, August 10th

Friday, August 11th

Saturday, August 12th

Sunday, August 13th

Monday, August 14th

Tuesday, August 15th

Wednesday, August 16th

Thursday, August 17th

Friday, August 18th.

Saturday, August 19th

Sunday, August 20th

Monday, August 21st

Tuesday, August 22nd

Wednesday, August 23rd

Thursday, August 24th

Friday, August 25th

Saturday, August 26th

Sunday, August 27th

Monday, August 28th

Tuesday, August 29th

Wednesday, August 30th

Thursday, August 31st

Friday, September 1st.

Saturday, September 2nd

Sunday, September 3rd

Monday, September 4th

Tuesday, September 5th

Wednesday, September 6th

Thursday, September 7th

Friday, September 8th

Saturday, September 9th

Sunday, September 10th

Monday, September 11th

Tuesday, September 12th

Wednesday, September 13th

Thursday, September 14th.

Friday, September 15th

Saturday, September 16th.

Sambutan Hari Malaysia!

Sunday, September 17th

Monday, September 18th

Tuesday, September 19th

Wednesday, September 20th

Thursday, September 21st

Friday, September 22nd

Saturday, September 23rd

Sunday, September 24th

Monday, September 25th

Tuesday, September 26th

Wednesday, September 27th

Thursday, September 28th

Friday, September 29th

Saturday, September 30th

Sunday, October 1st.

Monday, October 2nd.

Tuesday, October 3rd.

Wednesday, October 4th.

Thursday, October 5th.

Friday, October 6th.

Saturday, October 7th.

Sunday, October 8th.

Monday, October 9th.

Tuesday, October 10th.

Wednesday, October 11th.

Thursday, October 12th.

Friday, October 13th.

Saturday, October 14th.

Sunday, October 15th.

Monday, October 16th.

Tuesday, October 17th.

Wednesday, October 18th.

Thursday, October 19th.

Friday, October 20th.

Saturday, October 21st.

Sunday, October 22nd.

Monday, October 23rd.

Tuesday, October 24th.

Wednesday, October 25th.

Thursday, October 26th.

Friday, October 27th.

Saturday, October 28th.

Sunday, October 29th.

Monday, October 30th.

Tuesday, October 31st.

Wiki freeze coming in hot! *panic