Difference between revisions of "Team:WashU StLouis/Safety"

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<h1> Safety </h1>
 
<h1> Safety </h1>
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h2> Our Lab Space </h2>
  
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<p>Officially, our team operates under the Energy, Environmental, and Chemical Engineering (EECE) Department at Washington University in St. Louis. The majority of our wet lab work is done in the Tang and Zhang Labs, both of which are <strong>Biosafety Level 1 (BSL 1)</strong>. Both labs are equipped with open bench space and biosafety cabinets, and they share 4, 30, and 37 degree rooms for cell cultures and plates. The Zhang lab designates a specific area and enforces safety measures for work involving the use of Ethidium Bromide (such as in gel electrophoresis).</p>
  
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<h2> Safety Training </h2>
  
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<p>Prior to starting lab work, we were required to take mandatory online training and lab-specific safety training. The online training involved a slideshow and a quiz in order to receive certification from WashU's Environmental and Health Safety (EHS) department. Our lab-specific training was given by graduate students in the Zhang and Tang labs. During the lab-specific training, we learned the specific location of safety equipment and other materials as well as the various safety protocols instituted by both labs.</p>
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<h2>Personal Protective Equipment </h2>
  
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<p>Our PPE falls generally in line with the requirements of most BSL 1 laboratories. This means our attire entering the lab includes long pants, appropriate closed-toe shoes, and shirts with sleeves. Once we enter the lab, we make sure to put on fitting nitrile gloves, lab coats, and goggles/glasses (when necessary).</p>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Revision as of 07:00, 16 June 2017

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Safety

Our Lab Space

Officially, our team operates under the Energy, Environmental, and Chemical Engineering (EECE) Department at Washington University in St. Louis. The majority of our wet lab work is done in the Tang and Zhang Labs, both of which are Biosafety Level 1 (BSL 1). Both labs are equipped with open bench space and biosafety cabinets, and they share 4, 30, and 37 degree rooms for cell cultures and plates. The Zhang lab designates a specific area and enforces safety measures for work involving the use of Ethidium Bromide (such as in gel electrophoresis).

Safety Training

Prior to starting lab work, we were required to take mandatory online training and lab-specific safety training. The online training involved a slideshow and a quiz in order to receive certification from WashU's Environmental and Health Safety (EHS) department. Our lab-specific training was given by graduate students in the Zhang and Tang labs. During the lab-specific training, we learned the specific location of safety equipment and other materials as well as the various safety protocols instituted by both labs.

Personal Protective Equipment

Our PPE falls generally in line with the requirements of most BSL 1 laboratories. This means our attire entering the lab includes long pants, appropriate closed-toe shoes, and shirts with sleeves. Once we enter the lab, we make sure to put on fitting nitrile gloves, lab coats, and goggles/glasses (when necessary).