Difference between revisions of "Team:MSU-Michigan/Parts"

Line 48: Line 48:
 
</ul>
 
</ul>
 
</div>
 
</div>
 +
<br>
 +
<br>
 +
<br>
 +
 +
<p>The parts were taken from the Shewanella oneidensis MR-1 genome by finding a regulator on http://regprecise.lbl.gov/RegPrecise/.  Then taking the consensus sequence provided at the appropriate distance from a gene and taking the DNA that is upstream from the gene including the consensus sequence.  This ensured that the ribosomal binding site would be in the sequence we took as well as any other necessary machinery for the following gene to be regulated by the promoter that we extracted.  All of the promoters were then amplified using PCR and inserted into the prL814 plasmid in place of the T7A1 promoter.</p>
 +
  
 
<h2>Part Table </h2>
 
<h2>Part Table </h2>

Revision as of 18:36, 26 October 2017

Home
Description Design Experiments InterLab Contribution Results Demonstrate Attributions
Safety
Engagement Gold Integrated Silver
Team Collaborations
Parts
Applied Design Hardware Measurement Model

Parts

Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <groupparts> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.

Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.

Note

Note that parts must be documented on the Registry.

Adding parts to the registry

You can add parts to the Registry at our Add a Part to the Registry link.

What information do I need to start putting my parts on the Registry?

The information needed to initially create a part on the Registry is:

  • Part Name
  • Part type
  • Creator
  • Sequence
  • Short Description (60 characters on what the DNA does)
  • Long Description (Longer description of what the DNA does)
  • Design considerations



The parts were taken from the Shewanella oneidensis MR-1 genome by finding a regulator on http://regprecise.lbl.gov/RegPrecise/. Then taking the consensus sequence provided at the appropriate distance from a gene and taking the DNA that is upstream from the gene including the consensus sequence. This ensured that the ribosomal binding site would be in the sequence we took as well as any other necessary machinery for the following gene to be regulated by the promoter that we extracted. All of the promoters were then amplified using PCR and inserted into the prL814 plasmid in place of the T7A1 promoter.

Part Table

Biobricks

"Confirmation of promoters induced by (from left to right) Blue light, Paraquat, Nitrate (NO3), and Copper"

<groupparts>iGEM17 MSU-Michigan</groupparts>