Difference between revisions of "Team:HUST-China/Attributions"

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     <div class="tab1" id="section0" style="width:100%;margin-top: 65px;">
 
     <div class="tab1" id="section0" style="width:100%;margin-top: 65px;">
             <h1 class="tab-h1" ><strong>「Interlab」</strong></h1>
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             <h1 class="tab-h1" ><strong>「Attributions」</strong></h1>
 
     </div>
 
     </div>
  
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                 <div class="collapse navbar-collapse" id="navbar-collapse">
 
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                     <li class="active"><a href="#section1">Introduction</a></li>
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                     <li class="active"><a href="#section1">Project Design</a></li>
                     <li><a href="#section2">Provenance and Release</a></li>
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                     <li><a href="#section2">Wet Lab Work</a></li>
                     <li><a href="#section3">Chassis and Safety </a></li>
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                     <li><a href="#section3">Modeling </a></li>
                     <li><a href="#section4">Instrument</a></li>
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                     <li><a href="#section4">Wiki&Poster</a></li>
                     <li><a href="#section5">Calibration Protocol</a></li>  
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                     <li><a href="#section5">Human Practices</a></li>
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                    <li><a href="#section5">Advisors</a></li>
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                    <li><a href="#section5">Acknowledgments</a></li>
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                 </ul>
 
                 </ul>
 
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                 </div>   
 
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                 <div id="section1" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;">     
 
                 <div id="section1" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;">     
                     <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px;margin-top:0;">Introduction</h3>
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                     <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px;margin-top:0;">Project Design</h3>
                     <p style="padding:10px">It is significant for synthetic biology to develop a reliable and repeatable measurement, the same to all the other engineering disciplines. We HUST-China have volunteered to test some RBS devices (BCDs) that are intended to make gene expression more precise and reliable by measure the expression level of GFP, in order to help the iGEM community collect data about how reliable will these devices turn out to be in labs around the world.
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                     <p>All team members were involved in the design by Participating in brainstorming, querying literature and doing early experiment.
 
                     </p>
 
                     </p>
 
                 </div>
 
                 </div>
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                 <div id="section2" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;">  
 
                 <div id="section2" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;">  
                     <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Provenance and Release</h3>
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                     <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Wet Lab Work</h3>
                    <h4><strong>①Individuals responsible for conducting InterLab study</strong> </h4>
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                     <p>Xiao Deng, Yiyan Yu, Long Chen, Yubei Sun, Xiaoyang Li, Ziyang Xiao, Jie Mei, Haibo Huang,</p>
                     <div class="table-responsive" style="padding: 10px 100px; text-align: center;">
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                    <p>Zichun Weng, Yating Hu, Qingyang Yu, Huiping Shi, Kangyuan Yu, Nan Hu, Shaofeng Liao,</p>
                        <table class="table .table-hover">
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                    <p> Efan Wang, Ting Peng, Ziyi Li, Tongtong Zhu, Yuqi Wu, Xichen Bai.</p>  
                          <thead>
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                </div>
                              <tr>
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                                <th style="text-align: center;">Individuals</th>
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                                <th style="text-align: center;">Interlab Part</th>
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                              </tr>
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                          </thead>
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                          <tbody>
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                              <tr>
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                                <td>Kangyuan Yu, Haibo Huang, Ziyang Xiao, Shaofeng Liao</td>
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                                <td>created the devices</td>
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                              </tr>
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                              <tr>
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                                <td>Efan Wang, Long Cheng, HuiPing Shi </td>
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                                <td>conducted the measurements</td>
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                              </tr>
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                              <tr>
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                                <td>Efan Wang</td>
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                                <td>processed the data</td>
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                              </tr>
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                          </tbody>
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                        </table>
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                    </div>
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                    <h4><strong> ②Corresponding email</strong> </h4>
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                    <div class="table-responsive" style="padding: 10px 100px; text-align: center;">
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                        <table class="table .table-hover">
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                          <thead>
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                              <tr>
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                                <th style="text-align: center;">Individuals </th>
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                                <th style="text-align: center;">Emails</th>
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                              </tr>
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                          </thead>
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                          <tbody>
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                              <tr>
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                                <td>Efan Wang</td>
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                                <td>erfan@hust.edu.cn</td>
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                              </tr> 
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                              <tr>
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                                <td>Kangyuan Yu</td>
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                                <td>985930862@qq.com</td>
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                              </tr>
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                              <tr>
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                                <td>Haibo Huang </td>
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                                <td>u201512127@hust.edu.cn</td>
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                              </tr>
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                              <tr>
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                                <td>Ziyang Xiao</td>
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                                <td>372657289@qq.com</td>
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                              </tr>
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                              <tr>
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                                <td>Shaofeng Liao</td>
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                                <td> 15827233830@qq.com</td>
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                              </tr>
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                              <tr>
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                                <td>HuiPing Shi</td>
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                                <td>172295915@qq.com</td>
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                              </tr>
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                              <tr>
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                                <td>Long Cheng</td>
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                                <td>u201512127@hust.edu.cn</td>
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                              </tr>
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                          </tbody>
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                        </table>
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                    </div>
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                </div>       
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                 <div id="section3" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;">         
 
                 <div id="section3" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;">         
                     <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Chassis and Safety</h3>
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                     <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Modeling</h3>
                    <h4><strong> What chassis did you use?</strong> </h4>
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                     <p>Our modeling job are mainly taken in charge by Kai Kang and Yuanzhe Shao.</p>
                     <p>Escherichia coli DH5alpha</p>
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                    <h4><strong> What Biosafety Level is your chassis? </strong> </h4>
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                    <p>BSL1</p>
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                    <h4><strong>What PPE did you utilize during your experiments? </strong></h4>
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                    <p>Tianming gloves</p>
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                    <p>Songxinjiujiu labcoats</p>
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                 </div>
 
                 </div>
 
                 <div id="section4" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;">         
 
                 <div id="section4" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;">         
                     <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Instrument</h3>
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                     <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Wiki&Poster</h3>  
                     <h4><strong> What instrument did you use during your measurements? </strong> </h4>
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                     <p>All the content of our wiki was provided by all of us.</p>
                     <p>plate reader</p>
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                     <p>Wiki framework was done by Longhao Jia.</p>
                     <h4><strong> Please provide the brand and model of your instrument.</strong> </h4>
+
                     <p>Art and design was done by Wenjing Li.</p>
                    <p>Flexstation 3</p>
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                 </div>       
 
                 </div>       
                 <div id="section5" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;">        
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                 <div id="section5" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;">  
                     <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Calibration Protocol</h3>
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                     <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Human Practices</h3>
 +
                    <p>Yuanzhe Shao, Yating Hu, Ziyi Li, Yiyan Yu</p>     
 +
                </div>
 +
                <div id="section5" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;">
 +
                    <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Advisors</h3>
 +
                    <p>Professor Yunjun Yan</p>
 +
                    <p>Professor Kang Ning</p>
 +
                    <p>Dr Yi Zhan</p>
 +
                </div>
 +
                <div id="section5" style="border: solid 1px #666; margin:5px;border-radius:10px;overflow: hidden;">
 +
                    <h3 style="background-color:#ce6b9a;color:#ffffff; padding:10px;letter-spacing:1px; margin-top:0;">Acknowledgments</h3>
 +
                    <p>This project was supported by Huazhong University of science and technology, College of Life Science and Technology HUST, Qiming college of HUST.</p>
 +
                    <p>Mathworks provided software supply.</p>
 +
                    <p>TsingKe and TianYiHuiYuan provided primer synthesis and sequencing service.</p>
 +
                    <p>IDT provided DNA product.</p>
 +
                </div>
  
 
                    <h4><strong>  A1. Protocol for Optical Density (OD600) Standard Measurement</strong> </h4>
 
                    <h5><strong>Did you use pathlength correction during measurement? </strong> </h5>
 
                    <p>Yes</p>
 
                    <h5><strong>Number of flashes per well  </strong> </h5>
 
                    <p>6</p>
 
                    <h5><strong>Orbital averaging (mm)  </strong> </h5>
 
                    <p>600</p>
 
                    <h5><strong> What temperature setting did you use during the measurement? </strong> </h5>
 
                    <p>22℃</p>
 
                    <h5><strong>What type of 96-well plate did you use? </strong> </h5>
 
                    <p>Black plate (preferred)</p>
 
                    <h5><strong> Did your plate have flat-bottomed or round-bottomed wells? </strong> </h5>
 
                    <p>Flat</p>
 
 
 
                    <h4><strong> A2. Measurement Steps</strong></h4>
 
 
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                                                            Section 2
 
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                                        <li>① Prepare your 96 well plate</li>
 
                                        <li>②Add 100 μl LUDOX 100 % into wells A1, B1, C1, D1</li>
 
                                        <li>③Add 100 μl of dH2O into A2, B2, C2, D2</li>
 
                                        <li>④Measure absorbance 600 nm of all samples in all standard measurement modes in instrument</li>
 
                                        <li>⑤Import data into "Abs600" blue cells in provided Excel calibration sheet</li>
 
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                    <h4><strong>  B1. Protocol for FIuorescein Fluoresence standard curve</strong> </h4>
 
                    <h5><strong>Did you use pathlength correction during measurement? </strong> </h5>
 
                    <p>Yes</p>
 
                    <h5><strong>Number of flashes per well  </strong> </h5>
 
                    <p>6</p>
 
                    <h5><strong>What gain setting did you use?  </strong> </h5>
 
                    <p>Automatic</p>
 
                    <h5><strong> If you used a filter, what light wavelengths did it pass?  </strong> </h5>
 
                    <p>530nm</p>
 
                    <h5><strong>Emission wavelength </strong> </h5>
 
                    <p>530nm</p>
 
                    <h5><strong> Excitation wavelength </strong> </h5>
 
                    <p>485nm</p>
 
                    <h5><strong> Fluorescence reading  </strong> </h5>
 
                    <p>Bottom optic</p>
 
                    <h5><strong>What type of 96-well plate did you use? </strong> </h5>
 
                    <p>Black plate (preferred)</p>
 
                    <h5><strong> Did your plate have flat-bottomed or round-bottomed wells? </strong> </h5>
 
                    <p>Flat</p>
 
                    <h5><strong> What temperature setting did you use during the measurement?  </strong> </h5>
 
                    <p>22℃</p>
 
 
                    <h4><strong> B2. Measurement Steps</strong></h4>
 
                    <h5><strong>  Part 1: Prepare the Fluorescein stock solution </strong> </h5>
 
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                                    <li>①  Spin down fluorescein stock tube to make sure pellet is at the bottom of tube.</li>
 
                                    <li>②  Prepare 2x fluorescein stock solution (100 μM) by resuspending fluorescein in 1ml of 1xPBS</li>
 
                                    <li>③  Dilute the 2x fluorescein stock solution using 1xPBS to make a 1x fluorescein solution (final concentration is 50 μM).</li>
 
                                    <li>④  *Illustration of serial dilution samples in 96 well plate or cuvettes: value decreases by 2-fold with each column (50% in column 2, 25% in column 3, 12.5% in column 4, etc.)</li>
 
                                    <li>⑤Import data into "Abs600" blue cells in provided Excel calibration sheet</li>
 
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            <h3><strong>Acknowledgments:</strong></h3>
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Revision as of 13:19, 27 October 2017

Attributions

「Attributions」

Project Design

All team members were involved in the design by Participating in brainstorming, querying literature and doing early experiment.

Wet Lab Work

Xiao Deng, Yiyan Yu, Long Chen, Yubei Sun, Xiaoyang Li, Ziyang Xiao, Jie Mei, Haibo Huang,

Zichun Weng, Yating Hu, Qingyang Yu, Huiping Shi, Kangyuan Yu, Nan Hu, Shaofeng Liao,

Efan Wang, Ting Peng, Ziyi Li, Tongtong Zhu, Yuqi Wu, Xichen Bai.

Modeling

Our modeling job are mainly taken in charge by Kai Kang and Yuanzhe Shao.

Wiki&Poster

All the content of our wiki was provided by all of us.

Wiki framework was done by Longhao Jia.

Art and design was done by Wenjing Li.

Human Practices

Yuanzhe Shao, Yating Hu, Ziyi Li, Yiyan Yu

Advisors

Professor Yunjun Yan

Professor Kang Ning

Dr Yi Zhan

Acknowledgments

This project was supported by Huazhong University of science and technology, College of Life Science and Technology HUST, Qiming college of HUST.

Mathworks provided software supply.

TsingKe and TianYiHuiYuan provided primer synthesis and sequencing service.

IDT provided DNA product.

Acknowledgments: