Difference between revisions of "Team:MSU-Michigan/Parts"

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<p>The parts were taken from the Shewanella oneidensis MR-1 genome by finding a regulator on <a href="http://regprecise.lbl.gov/RegPrecise/">RegPrecise</a> Then taking the consensus sequence provided at the appropriate distance from a gene and taking the DNA that is upstream from the gene including the consensus sequence.  This ensured that the ribosomal binding site would be in the sequence we took as well as any other necessary machinery for the following gene to be regulated by the promoter that we extracted.  All of the promoters were then amplified using PCR and inserted into the prL814 plasmid in place of the T7A1 promoter.</p>
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<p>The parts were taken from the Shewanella oneidensis MR-1 genome by finding a regulator on <a href="http://regprecise.lbl.gov/RegPrecise/">RegPrecise.</a> Then the consensus sequence was located at the appropriate distance from a gene and DNA upstream from the gene was taken, which included the consensus sequence.  This ensured that the ribosomal binding site would be in the sequence we took as well as any other necessary machinery for the following gene to be regulated by the promoter that we extracted.  All of the promoters were then amplified using PCR and inserted into the prL814 plasmid in place of the T7A1 promoter.</p>
  
  

Revision as of 14:57, 27 October 2017

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Parts

The parts were taken from the Shewanella oneidensis MR-1 genome by finding a regulator on RegPrecise. Then the consensus sequence was located at the appropriate distance from a gene and DNA upstream from the gene was taken, which included the consensus sequence. This ensured that the ribosomal binding site would be in the sequence we took as well as any other necessary machinery for the following gene to be regulated by the promoter that we extracted. All of the promoters were then amplified using PCR and inserted into the prL814 plasmid in place of the T7A1 promoter.

Part Table

Biobricks

"Confirmation of promoters induced by (from left to right) Blue light, Paraquat, Nitrate (NO3), and Copper"

<groupparts>iGEM17 MSU-Michigan</groupparts>