Difference between revisions of "Team:Bielefeld-CeBiTec/Notebook/Oligonucleotides"

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</div>
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 +
 +
 +
 +
<div class="contentbox">
 +
<div class="content oligo-table">
 +
<table style="font-size: 10pt;">
 +
<thead>
 +
<th style="width: 55px;">ID</th>
 +
<th style="width: 180px;">Name</th>
 +
<th style="width: 100px;">Length</th>
 +
<th style="width: 80px;">Tm °C</th>
 +
<th style="width: auto;">Sequence 5'-> 3'</th>
 +
</thead>
 +
<tbody>
 +
<tr>
 +
    <td>17aa</td>
 +
    <td>tetM_gaF</td>
 +
    <td>48</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">ACAATGCTAGCGACAAAGAGGAGAAAGTAATGCGCACCCTGAACATCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ab</td>
 +
    <td>tetM_gaR</td>
 +
    <td>49</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTACTACTCACCCGAATGCCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ac</td>
 +
    <td>tetM_gaF</td>
 +
    <td>60</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">ACAATGCTAGCGACAAAGAGGAGAAAGTAATGAAAATCATAAATATCGGTGTATTAGCTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ad</td>
 +
    <td>tetM_gaR</td>
 +
    <td>60</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATGTGATTTTGTTGAACATATAACGAAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ae</td>
 +
    <td>tetM_gaF</td>
 +
    <td>64</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAAAATTATTAATATTGGAGTTTTAGCTCATG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17af</td>
 +
    <td>tetM_gaR</td>
 +
    <td>74</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTAAGTTATTTTATTGAACATATATCTTACTTTATCTATCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ag</td>
 +
    <td>pSB1C3-mRFP_gaF</td>
 +
    <td>49</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">CTAGTGTAGATCGCGGTCTCATGCGTACTAGTAGCGGCCGCTGCAGTCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ah</td>
 +
    <td>pSB1C3-mRFP_gaR</td>
 +
    <td>73</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">CTCCTCTTTGTCGCTAGCATTGTACCTAGGACTGAGCTAGCCATAAACTCTAGAAGCGGCCGCGAATTCCAGA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ai</td>
 +
    <td>aac2_gaF</td>
 +
    <td>49</td>
 +
    <td>62.0</td>
 +
    <td  style="font-size: 7.5pt;">TACAATGCTAGCGACAAAGAGGAGAAAGTAATGACCATCGACCTCCGCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17aj</td>
 +
    <td>aac2_gaR</td>
 +
    <td>48</td>
 +
    <td>62.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGGTGACGTCGCCGTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ak</td>
 +
    <td>mRFP_gaF</td>
 +
    <td>68</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CTAGGTACAATGCTAGCGACAAAGAGGAGAAAGTAATGGGCGAGACCGCTTCCTCCGAAGACGTTATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17al</td>
 +
    <td>mRFP_gaR</td>
 +
    <td>65</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTTGCCGGACTGCAGCGGCCGCTACTAGTACGCATGAGACCGCGATCTACACTAGCACTATCAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17am</td>
 +
    <td>aadA_gaF</td>
 +
    <td>49</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAGGGAAGCGGTGATCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17an</td>
 +
    <td>aadA_gaR</td>
 +
    <td>58</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTGCCGACTACCTTGGTGATCTCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ao</td>
 +
    <td>ermc_gaF</td>
 +
    <td>62</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">ACAATGCTAGCGACAAAGAGGAGAAAGTAATGAACGAGAAAAATATAAAACACAGTCAAAAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ap</td>
 +
    <td>ermc_gaR</td>
 +
    <td>78</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATTACTTATTAAATAATTTATAGCTATTGAAAAGAGATAAGAATTGTTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17aq</td>
 +
    <td>msrA_gaF</td>
 +
    <td>67</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">ACAATGCTAGCGACAAAGAGGAGAAAGTAATGGAACAATATACAATTAAATTTAACCAAATCAATCA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ar</td>
 +
    <td>msrA_gaR</td>
 +
    <td>67</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATTAAGTTATATCATGAATAGATTGTCCTGTTAATTCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17as</td>
 +
    <td>mef_gaF</td>
 +
    <td>62</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">ACAATGCTAGCGACAAAGAGGAGAAAGTAATGGAAAAATACAACAATTGGAAACTTAAGTTT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17at</td>
 +
    <td>mef_gaR</td>
 +
    <td>71</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTTAAATCTAATTTTCTAATCTCATTTATCATTGGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17au</td>
 +
    <td>ermX_gaF</td>
 +
    <td>48</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">ACAATGCTAGCGACAAAGAGGAGAAAGTAATGTCTGCATACGGACACG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17av</td>
 +
    <td>ermX_gaR</td>
 +
    <td>51</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATCACCTGGAAGAGATCGATCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17aw</td>
 +
    <td>carB4_gaF</td>
 +
    <td>54</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAAGTTGCTGTTAGTGTTCTCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ax</td>
 +
    <td>carB4_gar</td>
 +
    <td>53</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAACGGCTTTGCGAACTATAAA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ay</td>
 +
    <td>tetX_gaF</td>
 +
    <td>47</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">TGCTAGCGACAAAGAGGAGAAAGTAATGAGACCTCGTATCGCTGTGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17az</td>
 +
    <td>tetX_gaR</td>
 +
    <td>43</td>
 +
    <td>62.0</td>
 +
    <td  style="font-size: 7.5pt;">CGGACTGCAGCGGCCGCTACTAGTATCAAGCAGCAGGGCAGCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ba</td>
 +
    <td>addA1_gaF</td>
 +
    <td>55</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTGCCGACTACCTTGGTGATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bb</td>
 +
    <td>addA1_gaR</td>
 +
    <td>49</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAGGGAAGCGGTGATCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bc</td>
 +
    <td>addA1_gaF</td>
 +
    <td>49</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAGGGAAGCGGTGATCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bd</td>
 +
    <td>addA1_gaR</td>
 +
    <td>53</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTGCCGACTACCTTGGTGA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17be</td>
 +
    <td>aad9_gaF</td>
 +
    <td>52</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">GCTAGCGACAAAGAGGAGAAAGTAATGAGGAGGATATATTTGAATACATACG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bf</td>
 +
    <td>aad9_gaR</td>
 +
    <td>73</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">CGGACTGCAGCGGCCGCTACTAGTATTATAATTTTTTTAATCTGTTATTTAAATAGTTTATAGTTAAATTTAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bg</td>
 +
    <td>aac3_gaF</td>
 +
    <td>53</td>
 +
    <td>61.8</td>
 +
    <td  style="font-size: 7.5pt;">TACAATGCTAGCGACAAAGAGGAGAAAGTAGTGCAATACGAATGGCGAAAAGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bh</td>
 +
    <td>aac3_gaR</td>
 +
    <td>50</td>
 +
    <td>62.6</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGCCAATCGACTGGCGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bi</td>
 +
    <td>tetA_gaF</td>
 +
    <td>55</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAAATCTAACAATGCGCTCATCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bj</td>
 +
    <td>tetA_gaR</td>
 +
    <td>48</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATTAGGTCGAGGTGGCCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bk</td>
 +
    <td>pbp5_gaF</td>
 +
    <td>57</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TACAATGCTAGCGACAAAGAGGAGAAAGTAATGGAACGAAGCAATCGTAATAAAAAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bl</td>
 +
    <td>pbp5_gaR</td>
 +
    <td>65</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTAATGGTTGCTTCTAAGTAATCAATTAATG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bm</td>
 +
    <td>hyg_gaF</td>
 +
    <td>61</td>
 +
    <td>66.7</td>
 +
    <td  style="font-size: 7.5pt;">TACAATGCTAGCGACAAAGAGGAGAAAGTAGTGACACAAGAATCCCTGTTACTTCTCGACC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bn</td>
 +
    <td>hyg_gaR</td>
 +
    <td>46</td>
 +
    <td>67.9</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGGCGCCGGGGGCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bo</td>
 +
    <td>arpA_gaR</td>
 +
    <td>51</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGCCTTTCGGTGTTTCCAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bp</td>
 +
    <td>arpA_gaF</td>
 +
    <td>50</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">TACAATGCTAGCGACAAAGAGGAGAAAGTAATGCAATTCAAGCCAGCCGT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bq</td>
 +
    <td>ecoRI(1)35</td>
 +
    <td>35</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GAAGGTCGCCTGGAGTTCTCCGAAGTCGCGGTGGA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17br</td>
 +
    <td>ecoRI(1)re</td>
 +
    <td>35</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GCGACTTCGGAGAACTCCAGGCGACCTTCCTGCTT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bs</td>
 +
    <td>ecoRI(2)fw</td>
 +
    <td>36</td>
 +
    <td>64.0</td>
 +
    <td  style="font-size: 7.5pt;">CATTCTGATCGTGGAGTTCGCCAAGGAGCTGCACGA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bt</td>
 +
    <td>ecoRI(2)re</td>
 +
    <td>40</td>
 +
    <td>64.0</td>
 +
    <td  style="font-size: 7.5pt;">AGCTCCTTGGCGAACTCCACGATCAGAATGGCGTTTTTCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bu</td>
 +
    <td>PlacUV5_ga1F</td>
 +
    <td>55</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTACACTTTATGCTTCCGGCTCGTATAATGGACAAAGAGGAGAAAGTAATGGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bv</td>
 +
    <td>PlacUV5_ga1R</td>
 +
    <td>19</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CAGCGGCCGCTACTAGTAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bw</td>
 +
    <td>PlacUV5_ga2F</td>
 +
    <td>19</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">GTACTAGTAGCGGCCGCTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bx</td>
 +
    <td>PlacUV5_ga2R</td>
 +
    <td>48</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CATTATACGAGCCGGAAGCATAAAGTGTAAACTCTAGAAGCGGCCGCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17by</td>
 +
    <td>PtNTT2_gaF</td>
 +
    <td>47</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">GCTCGTATAATGGACAAAGAGGAGAAAGTAATGCGCCCGTATCCGAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17bz</td>
 +
    <td>PtNTT2_gaR</td>
 +
    <td>58</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTTTCTTTGTTGGTTTTCGCTTCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ca</td>
 +
    <td>LFS_ovexPCR_F</td>
 +
    <td>51</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">TCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17cb</td>
 +
    <td>LFS_ovexPCR_R</td>
 +
    <td>50</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">ACTGAGCCTTTCGTTTTATTTGATGCCTGGAATGCCTCCCGGATAAAACA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17cc</td>
 +
    <td>Term_ovexPCR_F</td>
 +
    <td>53</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTTCAGATGTGTTTTATCCGGGAGGCATTCCAGGCATCAAATAAAACGAAAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17cd</td>
 +
    <td>Term_ovexPCR_R</td>
 +
    <td>50</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">GAGCGCTCACAATTCTCACGCTTTCCAGTCTATAAACGCAGAAAGGCCCA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ce</td>
 +
    <td>lacO_ovexPCR_F</td>
 +
    <td>41</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">TGGGCCTTTCTGCGTTTATAGACTGGAAAGCGTGAGAATTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17cf</td>
 +
    <td>lacO_ovexPCR_R</td>
 +
    <td>57</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">ATCTAAGCCTATTGAGTATTTCTTATCCATCTAGTATTCCTGTGTGAAATTGTTATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17cg</td>
 +
    <td>cas9_ovexPCR_F</td>
 +
    <td>58</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">GCGGATAACAATTTCACACAGGAATACTAGATGGATAAGAAATACTCAATAGGCTTAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ch</td>
 +
    <td>cas9_ovexPCR_R</td>
 +
    <td>80</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">ATGGTAATGTTGCTCATATCAGTATCTCATTTTACGGCGGTCGCCTTGACCGCCGACACCCTCAGTCACCTCCTAGCTGA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ci</td>
 +
    <td>RFS_ovexPCR_F</td>
 +
    <td>80</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">GAGTCAGCTAGGAGGTGACTGAGGGTGTCGGCGGTCAAGGCGACCGCCGTAAAATGAGATACTGATATGAGCAACATTAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17cj</td>
 +
    <td>RFS_ovexPCR_R</td>
 +
    <td>59</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">TTAGAACAGGAATGGATTTTCTTCTACACACAATAAATGTAATGCGTTATATCTTCCTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ck</td>
 +
    <td>Sequ_ftsz_R</td>
 +
    <td>23</td>
 +
    <td>53.0</td>
 +
    <td  style="font-size: 7.5pt;">GTAAGTTCCATTGGTTCAAACAT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17cl</td>
 +
    <td>Sequ_ftsz_F</td>
 +
    <td>21</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">CTGCGTAAGCAAGCTGATTAA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17cm</td>
 +
    <td>Sequ_tet_R</td>
 +
    <td>21</td>
 +
    <td>53.0</td>
 +
    <td  style="font-size: 7.5pt;">GAGCGCATTGTTAGATTTCAT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17cn</td>
 +
    <td>Sequ_tet_F</td>
 +
    <td>20</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">GCCGGGCCACCTCGACCTAA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17co</td>
 +
    <td>Sequ_kan_R</td>
 +
    <td>21</td>
 +
    <td>50.0</td>
 +
    <td  style="font-size: 7.5pt;">CAATCCATCTTGTTCAATCAT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17cp</td>
 +
    <td>Sequ_kan_F</td>
 +
    <td>21</td>
 +
    <td>53.0</td>
 +
    <td  style="font-size: 7.5pt;">CTTCTTGACGAGTTCTTCTGA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17cq</td>
 +
    <td>codA_flanke1_delF</td>
 +
    <td>46</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TGAGTTAGCTCACTCATTAGGCACCCATTAAAAGCGGTCGTTCCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17cr</td>
 +
    <td>codA_flanke1_delR</td>
 +
    <td>27</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">TTATTCGACACTGTTAGCCTCCACATG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17cs</td>
 +
    <td>codA_flanke2_delF</td>
 +
    <td>44</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">TGTGGAGGCTAACAGTGTCGAATAAACGACTGGGTTACAGCGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ct</td>
 +
    <td>codA_flanke2_delR</td>
 +
    <td>45</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">GACTGGCTTTCTACGTGTTCCGCTTCGGAATTTGCCACCAGAGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17cu</td>
 +
    <td>sgRNA_LKA01_for</td>
 +
    <td>24</td>
 +
    <td>0.0</td>
 +
    <td  style="font-size: 7.5pt;">ACGCTATTGTTCATAATAGAAGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17cv</td>
 +
    <td>sgRNA_LKA01_rev</td>
 +
    <td>24</td>
 +
    <td>0.0</td>
 +
    <td  style="font-size: 7.5pt;">AAACCTCTTCTATTATGAACAATA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17cw</td>
 +
    <td>PtNTT2_assembly_gaF</td>
 +
    <td>20</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">GCGGCGGATAACGAACAGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17cx</td>
 +
    <td>PtNTT2_assembly_gaR</td>
 +
    <td>23</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">TAATGCTCTGTTCGTTATCCGCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17cy</td>
 +
    <td>Sequ_MCS_F</td>
 +
    <td>20</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">GCGAATTGGGTACACTTACC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17cz</td>
 +
    <td>Sequ_MCS_R</td>
 +
    <td>21</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">CACTAAAGGGAACAAAAGCTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17da</td>
 +
    <td>Sequ_pBK1_F</td>
 +
    <td>20</td>
 +
    <td>64.0</td>
 +
    <td  style="font-size: 7.5pt;">CACCTGACGCGCCCTGTAGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17db</td>
 +
    <td>Sequ_pBK2_F</td>
 +
    <td>24</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">GCATTCATTTTATGTTTCAGGTTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17dc</td>
 +
    <td>Sequ_pBK3_F</td>
 +
    <td>21</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">CAGCTTTTGTTCCCTTTAGTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17dd</td>
 +
    <td>Sequ_pBK4_F</td>
 +
    <td>21</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">CAGAAGAACTCGTCAAGAAGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17de</td>
 +
    <td>Sequ_54_F</td>
 +
    <td>22</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">GAACGCTCTCCTGAGTAGGACA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17df</td>
 +
    <td>Sequ_55_F</td>
 +
    <td>21</td>
 +
    <td>66.0</td>
 +
    <td  style="font-size: 7.5pt;">GGCGCAGCGGTCGGACTGAAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17dg</td>
 +
    <td>Sequ_56_F</td>
 +
    <td>21</td>
 +
    <td>64.0</td>
 +
    <td  style="font-size: 7.5pt;">CTGCCCTGCTGAACTACCGCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17dh</td>
 +
    <td>Sequ_58_R</td>
 +
    <td>21</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">GTAATCGCTGAATTCCCGGCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17di</td>
 +
    <td>Prefix_fwd</td>
 +
    <td>19</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTCTGGAATTCGCGGCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17dj</td>
 +
    <td>Suffix_rev</td>
 +
    <td>17</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17dk</td>
 +
    <td>KanMod-Fwd-1</td>
 +
    <td>57</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">CATAAACAGTAATACAAGGGGTGTTATGTAGAGCCATATTCAACGGGAAACGTCTTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17dl</td>
 +
    <td>KanMod-Fwd-2</td>
 +
    <td>60</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">CATAAACAGTAATACAAGGGGTGTTATGTAGTAGAGCCATATTCAACGGGAAACGTCTTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17dm</td>
 +
    <td>KanMod-Fwd-3</td>
 +
    <td>63</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">CATAAACAGTAATACAAGGGGTGTTATGTAGTAGTAGAGCCATATTCAACGGGAAACGTCTTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17dn</td>
 +
    <td>KanMod-Rev</td>
 +
    <td>32</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">AACACCCCTTGTATTACTGTTTATGTAAGCAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17do</td>
 +
    <td>KanMod-Split-Fwd</td>
 +
    <td>33</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">GTAGTGATCTTATTTCATTATGGTGAAAGTTGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17dp</td>
 +
    <td>KanMod-Split-Rev</td>
 +
    <td>33</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CCAACTTTCACCATAATGAAATAAGATCACTAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17dq</td>
 +
    <td>KanMod-19ML</td>
 +
    <td>24</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">GCTTAAACTCCAACTTGGACGCTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17dr</td>
 +
    <td>LacO_GA_For</td>
 +
    <td>52</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">ATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ds</td>
 +
    <td>LacO_GA_Rev</td>
 +
    <td>49</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">GGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17dt</td>
 +
    <td>VR</td>
 +
    <td>20</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">GCTCACTCAAAGGCGGTAAT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17du</td>
 +
    <td>VF2</td>
 +
    <td>20</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">TGCCACCTGACGTCTAAGAA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17dv</td>
 +
    <td>pK18-wo-ori_fwd</td>
 +
    <td>14</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">ACGCCAGCAACGCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17dw</td>
 +
    <td>pK18-wo-ori_rev</td>
 +
    <td>29</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">GAAGATCCTTTGATCTTTTCTACGGGGTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17dx</td>
 +
    <td>pSC101-replicon-gaF</td>
 +
    <td>45</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">CCGTAGAAAAGATCAAAGGATCTTCGAGTTATACACAGGGCTGGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17dy</td>
 +
    <td>pSC101-replicon_gaR</td>
 +
    <td>49</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">CGTAAAAAGGCCGCGTTGCTGGCGTTCAGATCCTTCCGTATTTAGCCAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17dz</td>
 +
    <td>RubiscoTAG2_fw</td>
 +
    <td>50</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">ATGCAGGATTACAAGCAAAGCCTCAAATATGAGACTTTCTCTTATCTTCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ea</td>
 +
    <td>RubiscoTAG2_rv</td>
 +
    <td>60</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">TGAGGCTTTGCTTGTAATCCTGCATCTAAGCCATGATATCTTTCTCCTCTTTCTCTAGAA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17eb</td>
 +
    <td>RubiscoTAG111_fw</td>
 +
    <td>52</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">AAAGAGGAGAAATACTAGATGGCAGTTAAGAAATATAGCGCGGGTGTCAAAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ec</td>
 +
    <td>RubiscoTAG111_rev</td>
 +
    <td>57</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">CTGCCATCTAGTATTTCTCCTCTTTGATATCTTACTAGTTGCCGCGGTAGACCACGA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ed</td>
 +
    <td>RubisoTAG474_fw</td>
 +
    <td>45</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">TACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ee</td>
 +
    <td>RubisoTAG474_rev</td>
 +
    <td>60</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">CGGACTGCAGCGGCCGCTACTAGTAGATATCTTACTAACGGTTTTGTGTGTCCAACTTAT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ef</td>
 +
    <td>RubisomRFP_fw</td>
 +
    <td>42</td>
 +
    <td>64.0</td>
 +
    <td  style="font-size: 7.5pt;">CTTCGTGGTCTACCGCGGCAACTACTAGAGAATGGCTTCCTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17eg</td>
 +
    <td>RubiscomRFP_rev</td>
 +
    <td>56</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GCGCTATATTTCTTAACTGCCATCTAGTATTTCTCCTCTTTGCGATCTACACTAGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17eh</td>
 +
    <td>mRFPRubisco_fw</td>
 +
    <td>36</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">GATAGTGCTAGTGTAGATCGCAAAGAGGAGAAATAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ei</td>
 +
    <td>mRFPRubisco_rev</td>
 +
    <td>33</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">GTCTTCGGAGGAAGCCATTCTCTAGTAGTTGCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ej</td>
 +
    <td>Sequ_pBAD_fw</td>
 +
    <td>18</td>
 +
    <td>62.0</td>
 +
    <td  style="font-size: 7.5pt;">GTTGCGTCGCGGTGCATG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ek</td>
 +
    <td>Sequ-pBAD_rev</td>
 +
    <td>20</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CAATCTCACCATTCGCGGTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17el</td>
 +
    <td>VR</td>
 +
    <td>20</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">ATTACCGCCTTTGAGTGAGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17em</td>
 +
    <td>LFS_GA_F</td>
 +
    <td>64</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCATTGCCAGAGTGAAAATGCCTCAGAGCT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17en</td>
 +
    <td>LFS_GA_R</td>
 +
    <td>74</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">CCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAAATGCCTCCCGGATAAAACACATCTGAAAATTCATATGT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17eo</td>
 +
    <td>BB_LFS_F</td>
 +
    <td>56</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTTCAGATGTGTTTTATCCGGGAGGCATTTACTAGTAGCGGCCGCTGCAGTCCGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ep</td>
 +
    <td>BB_LFS_R</td>
 +
    <td>64</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGCTCTGAGGCATTTTCACTCTGGCAATGCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17eq</td>
 +
    <td>Term_GA_F</td>
 +
    <td>57</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">ATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCCAGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17er</td>
 +
    <td>Term_GA_R</td>
 +
    <td>67</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">GGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17es</td>
 +
    <td>BB_Term_F</td>
 +
    <td>56</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">CACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTCCGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17et</td>
 +
    <td>BB_Term_R</td>
 +
    <td>64</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">ACTGAGCCTTTCGTTTTATTTGATGCCTGGCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17eu</td>
 +
    <td>lacO_GA_F2</td>
 +
    <td>62</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">ATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGACTGGAAAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ev</td>
 +
    <td>lacO_GA_R2</td>
 +
    <td>67</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">GGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTCCTGTGTGAAATTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ew</td>
 +
    <td>BB_lacO_F</td>
 +
    <td>56</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">TTGTGAGCGGATAACAATTTCACACAGGAATACTAGTAGCGGCCGCTGCAGTCCGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ex</td>
 +
    <td>BB_lacO_R</td>
 +
    <td>64</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">GAGCGCTCACAATTCTCACGCTTTCCAGTCCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ey</td>
 +
    <td>Cas9_GA_F</td>
 +
    <td>69</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGATGGATAAGAAATACTCAATAGGCTTAGATATCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ez</td>
 +
    <td>Cas9_GA_R</td>
 +
    <td>80</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">GCCGGACTGCAGCGGCCGCTACTAGTATTTTACGGCGGTCGCCTTGACCGCCGACACCCTCAGTCACCTCCTAGCTGACT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fa</td>
 +
    <td>BB_Cas9_F</td>
 +
    <td>80</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">GAGTCAGCTAGGAGGTGACTGAGGGTGTCGGCGGTCAAGGCGACCGCCGTAAAATACTAGTAGCGGCCGCTGCAGTCCGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fb</td>
 +
    <td>BB_Cas9_R</td>
 +
    <td>64</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">ATCTAAGCCTATTGAGTATTTCTTATCCATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fc</td>
 +
    <td>RFS_GA_F</td>
 +
    <td>74</td>
 +
    <td>62.0</td>
 +
    <td  style="font-size: 7.5pt;">AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTGAGATACTGATATGAGCAACATTACCATTTATCACAAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fd</td>
 +
    <td>RFS_GA_R</td>
 +
    <td>80</td>
 +
    <td>62.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTACAATAAATGTAATGCGTTATATCTTCCTGATATAATTACCTTTGATTTCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fe</td>
 +
    <td>BB_RFS_F</td>
 +
    <td>56</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">TCAGGAAGATATAACGCATTACATTTATTGTACTAGTAGCGGCCGCTGCAGTCCGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ff</td>
 +
    <td>BB_RFS_R</td>
 +
    <td>64</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">AATGGTAATGTTGCTCATATCAGTATCTCACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fg</td>
 +
    <td>PtNTT2_trunc_gaF</td>
 +
    <td>45</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">TCGTATAATGGACAAAGAGGAGAAAGTAATGGCGGCAGCACCGTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fh</td>
 +
    <td>aaRS_pC3_fw</td>
 +
    <td>37</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">CGGCCGCTTCTAGAGGCACCTGAAGTCAGCCCCATAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fi</td>
 +
    <td>aaRS_RuTAG_rev</td>
 +
    <td>40</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">TATCTTTCTCCTCTTTGTGCAAGATTCCGAATACCGCAAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fj</td>
 +
    <td>RuTAG_aaRS_fw</td>
 +
    <td>38</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">TTCGGAATCTTGCACAAAGAGGAGAAAGATATCATGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fk</td>
 +
    <td>RuTAG474_pC3_rv</td>
 +
    <td>40</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">CGGCCGCTACTAGTAGATATCTTACTAACGGTTTTGTGTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fl</td>
 +
    <td>pC3_RuTAG474_fw</td>
 +
    <td>45</td>
 +
    <td>70.0</td>
 +
    <td  style="font-size: 7.5pt;">CGTTAGTAAGATATCTACTAGTAGCGGCCGCTGCAGTCCGGCAAA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fm</td>
 +
    <td>pC3_aaRS_rev</td>
 +
    <td>55</td>
 +
    <td>70.0</td>
 +
    <td  style="font-size: 7.5pt;">GCTGACTTCAGGTGCCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fn</td>
 +
    <td>pC3_RuTAG_fw</td>
 +
    <td>45</td>
 +
    <td>70.0</td>
 +
    <td  style="font-size: 7.5pt;">CACACAAAACCGTTAATACTAGTAGCGGCCGCTGCAGTCCGGCAA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fo</td>
 +
    <td>Sequ_pBad3</td>
 +
    <td>20</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">GCACCACCCGGATGCCTATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fp</td>
 +
    <td>Sequ_pBad4</td>
 +
    <td>21</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">GTCCGCACCGCTGCCGGTAGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fq</td>
 +
    <td>RuTAG_pC3_rv</td>
 +
    <td>37</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">TTGGACACACAAAACCGTTAATACTAGTAGCGGCCGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fr</td>
 +
    <td>pSB1C3_splitF</td>
 +
    <td>20</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">GTCTGACAGCTCGAGGCTTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fs</td>
 +
    <td>pSB1C3_splitR</td>
 +
    <td>20</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">CAAGCCTCGAGCTGTCAGAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ft</td>
 +
    <td>PlacUV5_ga2R_v2</td>
 +
    <td>56</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">TCTTTGTCCATTATACGAGCCGGAAGCATAAAGTGTAAACTCTAGAAGCGGCCGCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fu</td>
 +
    <td>PlacUV5_ga2F_v2</td>
 +
    <td>18</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">TACTAGTAGCGGCCGCTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fv</td>
 +
    <td>pRedET_SeqF_1</td>
 +
    <td>20</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">AGGCGTAGCAGAAACTAACG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fw</td>
 +
    <td>pRedET_SeqF_2</td>
 +
    <td>20</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">CACACAAAGGTCTAGCGGAA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fx</td>
 +
    <td>pRedET_SeqR_1</td>
 +
    <td>18</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">GACGCAGTGACGGCAATG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fy</td>
 +
    <td>pRedET_SeqR_2</td>
 +
    <td>20</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">ATTTCAGCGACCTGTTTGGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17fz</td>
 +
    <td>pK18msb_ga1</td>
 +
    <td>20</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">AAGCGGAACACGTAGAAAGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ga</td>
 +
    <td>pK18msb_ga2</td>
 +
    <td>22</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">GGTGCCTAATGAGTGAGCTAAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gb</td>
 +
    <td>K1172904fw_gb1</td>
 +
    <td>51</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">GATTTCTGGAATTCGCGGCCGCTTCTAGAGATGGCAGCACAGGTTATCAAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gc</td>
 +
    <td>K1172904re_gb2</td>
 +
    <td>60</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATCTGATTTTTGTAAAGGTCTGATAATG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gd</td>
 +
    <td>psB1A3fw_gb3</td>
 +
    <td>51</td>
 +
    <td>64.0</td>
 +
    <td  style="font-size: 7.5pt;">CATTATCAGACCTTTACAAAAATCAGATAATACTAGTAGCGGCCGCTGCAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ge</td>
 +
    <td>psB1A3re_gb4</td>
 +
    <td>51</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GTCAAACGTGTTGATAACCTGTGCTGCCATCTCTAGAAGCGGCCGCGAATT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gf</td>
 +
    <td>Glu107_fw</td>
 +
    <td>63</td>
 +
    <td>67.0</td>
 +
    <td  style="font-size: 7.5pt;">CTGAAAGCGAAATACGTCTATGGCAGCNNKGAACAGCTGGACAAAGACTACACCCTGAACGTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gg</td>
 +
    <td>Glu107_rev</td>
 +
    <td>64</td>
 +
    <td>67.0</td>
 +
    <td  style="font-size: 7.5pt;">GCTGCCATAGACGTATTTCGCTTTCAGGCCCATCGCTTCGAACACTTTCTTGTTGTAGTCGCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gh</td>
 +
    <td>Asp158_Ile159_Leu162_fw</td>
 +
    <td>73</td>
 +
    <td>67.0</td>
 +
    <td  style="font-size: 7.5pt;">GAAGTGATCTATCCGATCATGCAGGTGAACNNKNNKCACTATNNKGGCGTAGATGTAGCAGTTGGTGGCATGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gi</td>
 +
    <td>Asp158_Ile159_Leu162_rev</td>
 +
    <td>59</td>
 +
    <td>67.0</td>
 +
    <td  style="font-size: 7.5pt;">GTTCACCTGCATGATCGGATAGATCACTTCCGCCACTTTCGGGTTTTCATCTTCGCGGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gj</td>
 +
    <td>pSB1C3_lin_fwd</td>
 +
    <td>19</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TACTAGTAGCGGCCGCTGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gk</td>
 +
    <td>GFPBB_fwd</td>
 +
    <td>51</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CTGGAATTCGCGGCCGCTTCTAGAGATGCGTAAAGGAGAAGAACTTTTCAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gl</td>
 +
    <td>pSB1C3_lin_rev</td>
 +
    <td>17</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">CTCTAGAAGCGGCCGCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gm</td>
 +
    <td>GFPLink1_rev</td>
 +
    <td>64</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CCCACCACTGCCTCCACTACCTCCAGAGCCCCCTTTGTATAGTTCATCCATGCCATGTGTAATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gn</td>
 +
    <td>GFPLink2_rev</td>
 +
    <td>64</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CCCACCACTGCCTCCCTAACCTCCAGAGCCCCCTTTGTATAGTTCATCCATGCCATGTGTAATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17go</td>
 +
    <td>Link1Step_fwd</td>
 +
    <td>54</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">GGGGGCTCTGGAGGTAGTGGAGGCAGTGGTGGGGCTGAAGCTGGTATCACCGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gp</td>
 +
    <td>Link2Step_fwd</td>
 +
    <td>54</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">GGGGGCTCTGGAGGTTAGGGAGGCAGTGGTGGGGCTGAAGCTGGTATCACCGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gq</td>
 +
    <td>StrepBB_rev</td>
 +
    <td>45</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">CTGCAGCGGCCGCTACTAGTATTAGGAAGCAGCGGACGGTTTAAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gr</td>
 +
    <td>sup35_Histag_fw</td>
 +
    <td>66</td>
 +
    <td>70.0</td>
 +
    <td  style="font-size: 7.5pt;">CATCATCACCATCACCACCATCATCACCATTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gs</td>
 +
    <td>sup35_Histag_rev</td>
 +
    <td>67</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">GATGGTGGTGATGGTGATGATGAACTTCGTCGTCAACTTCTTCTTCCTGTTCTTTGATCAGAGCGTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gt</td>
 +
    <td>pSB1C3_sup35_fw</td>
 +
    <td>51</td>
 +
    <td>70.0</td>
 +
    <td  style="font-size: 7.5pt;">GAAGTTGACGACGAAGTTTAATACTAGTAGCGGCCGCTGCAGTCCGGCAAA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gu</td>
 +
    <td>pSB1C3_sup35_rv</td>
 +
    <td>56</td>
 +
    <td>70.0</td>
 +
    <td  style="font-size: 7.5pt;">CCTGGTTAGAGTCAGACATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gv</td>
 +
    <td>T7RNAP_fw</td>
 +
    <td>59</td>
 +
    <td>64.0</td>
 +
    <td  style="font-size: 7.5pt;">GATTTCTGGAATTCGCGGCCGCTTCTAGAGATGAACACGATTAACATCGCTAAGAACGA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gw</td>
 +
    <td>T7RNAP_re</td>
 +
    <td>50</td>
 +
    <td>64.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATTACGCGAACGCGAAGTCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gx</td>
 +
    <td>psB1C3_T7P_fw</td>
 +
    <td>50</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">ATCTTAGAGTCGGACTTCGCGTTCGCGTAATACTAGTAGCGGCCGCTGCA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gy</td>
 +
    <td>psB1C3_T7P_re</td>
 +
    <td>50</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GTCGTTCTTAGCGATGTTAATCGTGTTCATCTCTAGAAGCGGCCGCGAAT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17gz</td>
 +
    <td>PtNTT2_seq_1</td>
 +
    <td>20</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">GGCAACCAGGAAGTGACCAA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ha</td>
 +
    <td>PtNTT2_seq_2</td>
 +
    <td>20</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">CACATCAATCGCCGCTTTGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hb</td>
 +
    <td>PromRBS-Lin-fwd</td>
 +
    <td>25</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">TACTAGTAGCGGCCGCTGCAGTCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hc</td>
 +
    <td>KanR-Prä-fwd</td>
 +
    <td>54</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">ATGCTAGCTACTAGAGTCACACAGGAAAGATGAGCCATATTCAACGGGAAACGT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hd</td>
 +
    <td>PromRBS-Lin-rev</td>
 +
    <td>23</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">CTTTCCTGTGTGACTCTAGTAGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17he</td>
 +
    <td>KanMod-19ML-fwd</td>
 +
    <td>43</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">TCAGCATCCAAGTTGGAATTTAATCGCGGCCTGGAGCAAGACG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hf</td>
 +
    <td>KanMod-19ML-rev</td>
 +
    <td>43</td>
 +
    <td>53.0</td>
 +
    <td  style="font-size: 7.5pt;">GCGATTAAATTCCAACTTGGATGCTGATTTATATGGGTATAAA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hg</td>
 +
    <td>Suff-KanR-rev</td>
 +
    <td>52</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TGCAGCGGCCGCTACTAGTAATTAGAAAAACTCATCGAGCATCAAATGAAAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hh</td>
 +
    <td>neuKan-mod-TAG1</td>
 +
    <td>49</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">ACTAGAGTCACACAGGAAAGATGTAGAGCCATATTCAACGGGAAACGTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hi</td>
 +
    <td>neuKan-mod-TAG2</td>
 +
    <td>52</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">ACTAGAGTCACACAGGAAAGATGTAGTAGAGCCATATTCAACGGGAAACGTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hj</td>
 +
    <td>neuKan-mod-TAG3</td>
 +
    <td>55</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">ACTAGAGTCACACAGGAAAGATGTAGTAGTAGAGCCATATTCAACGGGAAACGTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hk</td>
 +
    <td>ONBY-out-rev</td>
 +
    <td>26</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">ATGAATCTCCAAGTATATAACGACTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hl</td>
 +
    <td>ONBY-out-fwd</td>
 +
    <td>27</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">TAAGATCTGGATCCTCTACGCCGGACG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hm</td>
 +
    <td>NPA/ONBY-switch-fwd</td>
 +
    <td>46</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TTATATACTTGGAGATTCATATGGACGAGTTTGAGATGATTAAGCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hn</td>
 +
    <td>ONBY/NPA-switch-rev</td>
 +
    <td>47</td>
 +
    <td>64.0</td>
 +
    <td  style="font-size: 7.5pt;">TCCGGCGTAGAGGATCCAGATCTTACAGGCGTTTGCGGATTGGTTCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ho</td>
 +
    <td>pK18msb_seq_1</td>
 +
    <td>20</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGCTATCGCCATGTAAGCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hp</td>
 +
    <td>pK18msb_seq_2</td>
 +
    <td>20</td>
 +
    <td>62.0</td>
 +
    <td  style="font-size: 7.5pt;">AGCGGAAGAGCGCCCAATAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hq</td>
 +
    <td>aaRS_pSB1C3_fw</td>
 +
    <td>50</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CGGCCGCTTCTAGAGGATTATCAATTTTAAAAAACTAACAGTTGTCAGCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hr</td>
 +
    <td>aaRS_pSB1C3_rev</td>
 +
    <td>39</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">GCAGCGGCCGCTACTAGTACTCGAGCATGCAAAAAAGCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hs</td>
 +
    <td>pSB1C3_aaRS_fw</td>
 +
    <td>44</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">GCTTTTTTGCATGCTCGAGTACTAGTAGCGGCCGCTGCAGTCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ht</td>
 +
    <td>pSB1C3_aaRS_rev</td>
 +
    <td>50</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">GTTTTTTAAAATTGATAATCCTCTAGAAGCGGCCGCGAATTCCAGAAATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hu</td>
 +
    <td>sgRNA_romA_1_fw</td>
 +
    <td>40</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">CGTCAGTCCAGATACCTTTGATGTCCTGATTGGAAGGACC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hv</td>
 +
    <td>sgRNA_romA_1_rv</td>
 +
    <td>40</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">ACTCCTCCTCTCAAGGACTATAGAAAAAAAGTCCCGCTGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hw</td>
 +
    <td>sgRNA_romG_2_fw</td>
 +
    <td>40</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">GCAGCGGGACTTTTTTTCTATAGTCCTTGAGAGGAGGAGT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hx</td>
 +
    <td>sgRNA_romG_2_rv</td>
 +
    <td>40</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">GCTCACAGGACACCTGGTATACGTATTGATCTCACGAGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hy</td>
 +
    <td>sgRNA_romd_3_fw</td>
 +
    <td>40</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">GCCTCGTGAGATCAATACGTATACCAGGTGTCCTGTGAGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17hz</td>
 +
    <td>sgRNA_romd_3_rv</td>
 +
    <td>40</td>
 +
    <td>67.0</td>
 +
    <td  style="font-size: 7.5pt;">GATTATCGGGCAAATACCTTCTCATACGTGACGGATTCCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ia</td>
 +
    <td>sgRNA_romT_4_fw</td>
 +
    <td>40</td>
 +
    <td>67.0</td>
 +
    <td  style="font-size: 7.5pt;">GGGAATCCGTCACGTATGAGAAGGTATTTGCCCGATAATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ib</td>
 +
    <td>sgRNA_romT_4_rv</td>
 +
    <td>40</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">ATACGTTCCCTTTAATTGCCTGCCGGAGTTAAGGACGTTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ic</td>
 +
    <td>sgRNA_romC_5_fw</td>
 +
    <td>40</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">GAACGTCCTTAACTCCGGCAGGCAATTAAAGGGAACGTAT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17id</td>
 +
    <td>sgRNA_romC_5_rv</td>
 +
    <td>40</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">GCTAGTATTACTCTGATCTGATGTTTGCAGTCGCTGCTGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ie</td>
 +
    <td>Prefix_rev</td>
 +
    <td>17</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">CTCTAGAAGCGGCCGCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17if</td>
 +
    <td>pSC101-ori-part_gaF</td>
 +
    <td>45</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">CTGGAATTCGCGGCCGCTTCTAGAGGAGTTATACACAGGGCTGGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ig</td>
 +
    <td>pSC101-ori-part_gaR</td>
 +
    <td>49</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CGGACTGCAGCGGCCGCTACTAGTATCAGATCCTTCCGTATTTAGCCAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ih</td>
 +
    <td>sgRNAs_Rom_gaF</td>
 +
    <td>66</td>
 +
    <td>62.0</td>
 +
    <td  style="font-size: 7.5pt;">GGCCTTTCTGCGTTTATATACTAGAAGCGGCCGCTGCACGTCAGTCCAGATACCTTTGATGTCCTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ii</td>
 +
    <td>sgRNAs_Rom_gaR</td>
 +
    <td>78</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">AACTAACTAACCCTTAGTGACTCCTGCAGCGGCCGCTACTAGTAGCTAGTATTACTCTGATCTGATGTTTGCAGTCGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ij</td>
 +
    <td>sgRNA_Rom_BB_gF</td>
 +
    <td>32</td>
 +
    <td>62.0</td>
 +
    <td  style="font-size: 7.5pt;">GCTGCAGGAGTCACTAAGGGTTAGTTAGTTAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ik</td>
 +
    <td>sgRNA_Rom_BB_gR</td>
 +
    <td>32</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">CCGCTTCTAGTATATAAACGCAGAAAGGCCCA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17il</td>
 +
    <td>SeqCas1</td>
 +
    <td>20</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">ATTTGGCCTTAGCGCATATG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17im</td>
 +
    <td>SeqCas2</td>
 +
    <td>20</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">GGCTCCCCTATCAGCTTCAA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17in</td>
 +
    <td>SeqCas3</td>
 +
    <td>21</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">TCGATAAAGGTGCTTCAGCTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17io</td>
 +
    <td>SeqCas4</td>
 +
    <td>20</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">GGTTGGGGACGTTTGTCTCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ip</td>
 +
    <td>SeqCas5</td>
 +
    <td>22</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">GGAAGAGACATGTATGTGGACC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17iq</td>
 +
    <td>SeqCas6</td>
 +
    <td>20</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">GCCGTCGTTGGAACTGCTTT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ir</td>
 +
    <td>SeqCas7</td>
 +
    <td>21</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">CAGTCCTAGTGGTTGCTAAGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17is</td>
 +
    <td>SeqCas8</td>
 +
    <td>20</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">AATCTTGGAGCTCCCGCTGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17it</td>
 +
    <td>Seq_LFS</td>
 +
    <td>20</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">CGTCCTGATTCAGACCTCCT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17iu</td>
 +
    <td>Seq_B0015</td>
 +
    <td>20</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">GGCTCAGTCGAAAGACTGGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17iv</td>
 +
    <td>RuBisCo_RFP_fw</td>
 +
    <td>56</td>
 +
    <td>64.0</td>
 +
    <td  style="font-size: 7.5pt;">GCTAGTGTAGATCGCAAAGAGGAGAAATACTAGATGGCAGTTAAGAAATATAGCGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17iw</td>
 +
    <td>Rubisco_RFP_rev</td>
 +
    <td>37</td>
 +
    <td>64.0</td>
 +
    <td  style="font-size: 7.5pt;">AGCCATTCTCTAGTAGTTGCCGCGGTAGACCACGAAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ix</td>
 +
    <td>RFP_RuBisCo_rev</td>
 +
    <td>49</td>
 +
    <td>64.0</td>
 +
    <td  style="font-size: 7.5pt;">GTATTTCTCCTCTTTGCGATCTACACTAGCACTATCAGCGTTATTAAGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17iy</td>
 +
    <td>RFP_RuBisCo_fw</td>
 +
    <td>43</td>
 +
    <td>64.0</td>
 +
    <td  style="font-size: 7.5pt;">GGCAACTACTAGAGAATGGCTTCCTCCGAAGACGTTATCAAAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17iz</td>
 +
    <td>ori_pSB1C3_fw_66_3</td>
 +
    <td>39</td>
 +
    <td>66.0</td>
 +
    <td  style="font-size: 7.5pt;">CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ja</td>
 +
    <td>pSB1C3_ori_rev_66_2</td>
 +
    <td>33</td>
 +
    <td>66.0</td>
 +
    <td  style="font-size: 7.5pt;">CGGAGCCTATGGAAAAACGCCAGCAACGCGGCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jb</td>
 +
    <td>pSB1C3_ori_fw_65_4</td>
 +
    <td>54</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">CAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jc</td>
 +
    <td>ori_pSB1C3_rev_65_3</td>
 +
    <td>57</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">GTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jd</td>
 +
    <td>J23100_gaF</td>
 +
    <td>43</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">CTTAGTGACTCGAATTCGCGGCCGCTTCTAGAGTTGACGGCTA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17je</td>
 +
    <td>J23100_gaR</td>
 +
    <td>56</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">TAACTAACTAACCCTTAGTGACTCCTGCAGCGGCCGCTACTAGTATATAAACGCAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jf</td>
 +
    <td>sgRNA_BB_gaF</td>
 +
    <td>56</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">CTGCAAACATCAGATCAGAGTAATACTAGCTACTAGTAGCGGCCGCTGCAGGAGTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jg</td>
 +
    <td>sgRNA_BB_gaR</td>
 +
    <td>62</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">ATCAGGACATCAAAGGTATCTGGACTGACGTATAAACGCAGAAAGGCCCACCCGAAGGTGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jh</td>
 +
    <td>retBB_Rom_gaR</td>
 +
    <td>38</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">CTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ji</td>
 +
    <td>retBB_Rom_gaF</td>
 +
    <td>44</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">ATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCTTCCTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jj</td>
 +
    <td>pSB1C3_aaRS(oT)_fw_66</td>
 +
    <td>55</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">ATTTTAGAGCCAATTAGAAAGAGATTATAATACTAGTAGCGGCCGCTGCAGTCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jk</td>
 +
    <td>aaRS(oT)_pSB1C3_rev_58</td>
 +
    <td>71</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CCGGACTGCAGCGGCCGCTACTAGTATTATAATCTCTTTCTAATTGGCTCTAAAATCTTTATAAGTTCTTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jl</td>
 +
    <td>ori_pSB1C3_fw_65</td>
 +
    <td>56</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">TAAAAAGGCCGCGTTGCTGGCGTTGTGACCAAACAGGAAAAAACCGCCCTTAACAT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jm</td>
 +
    <td>neu_pSB1C3_ori_fw_58</td>
 +
    <td>51</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">TACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jn</td>
 +
    <td>pSB1C3_ori_rev_65</td>
 +
    <td>40</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">GCGGTTTTTTCCTGTTTGGTCACAACGCCAGCAACGCGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jo</td>
 +
    <td>KanR-mod-fwd (80)</td>
 +
    <td>80</td>
 +
    <td>72.0</td>
 +
    <td  style="font-size: 7.5pt;">ACTAGAGTCACACAGGAAAGATGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCCGCTTGGGTTGAGAGGCTATTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jp</td>
 +
    <td>KanR-mod-rev (80)</td>
 +
    <td>80</td>
 +
    <td>72.0</td>
 +
    <td  style="font-size: 7.5pt;">CTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGAAGAACTCGTCAAGAAGGCGATAGAAGGCGATGCGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jq</td>
 +
    <td>KanR-TAG1-fwd (73)</td>
 +
    <td>73</td>
 +
    <td>71.0</td>
 +
    <td  style="font-size: 7.5pt;">AATGCTAGCTACTAGAGTCACACAGGAAAGATGTAGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jr</td>
 +
    <td>KanR-TAG2-fwd (76)</td>
 +
    <td>76</td>
 +
    <td>71.0</td>
 +
    <td  style="font-size: 7.5pt;">AATGCTAGCTACTAGAGTCACACAGGAAAGATGTAGTAGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17js</td>
 +
    <td>KanR-TAG3-fwd (79)</td>
 +
    <td>79</td>
 +
    <td>71.0</td>
 +
    <td  style="font-size: 7.5pt;">AATGCTAGCTACTAGAGTCACACAGGAAAGATGTAGTAGTAGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jt</td>
 +
    <td>KanR-mod-fwd-short (59)</td>
 +
    <td>59</td>
 +
    <td>71.0</td>
 +
    <td  style="font-size: 7.5pt;">ACTAGAGTCACACAGGAAAGATGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ju</td>
 +
    <td>pSB1C3_sup_fw</td>
 +
    <td>70</td>
 +
    <td>70.0</td>
 +
    <td  style="font-size: 7.5pt;">GATCAAAGAACAGGAAGAAGAAGTTGACGACGAAGTTTAATACTAGTAGCGGCCGCTGCAGTCCGGCAAA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jv</td>
 +
    <td>pSB1C3_sup_rev</td>
 +
    <td>68</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">GCTGGTTGTTACCCTGGTTAGAGTCAGACATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jw</td>
 +
    <td>sup35_121CUA_fw</td>
 +
    <td>50</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">CCAGGCTGGTTTCCAGCCGCAGCTACAGGGTATGTCTCTGAACGACTTCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jx</td>
 +
    <td>sup35_121CUA_rv</td>
 +
    <td>55</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGCTGCGGCTGGAAACCAGCCTGGTAACCTTGCAGGTTGTTGTTGTAGTTGAAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jy</td>
 +
    <td>AcF_CUA_fw</td>
 +
    <td>54</td>
 +
    <td>67.0</td>
 +
    <td  style="font-size: 7.5pt;">CGGACTTAGAATCCGCATGGCAGGGGTTCAAATCCCCTCCGCCGGACCAAATTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17jz</td>
 +
    <td>AcF_CUA_rev</td>
 +
    <td>50</td>
 +
    <td>67.0</td>
 +
    <td  style="font-size: 7.5pt;">CCCTGCCATGCGGATTCTAAGTCCGCCGTTCTGCCCTGCTGAACTACCGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ka</td>
 +
    <td>AB_BB_gaF</td>
 +
    <td>33</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">TACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17kb</td>
 +
    <td>AB_BB_gaR</td>
 +
    <td>48</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">TACTTTCTCCTCTTTGTCGCTAGCATTGTACCTAGGACTGAGCTAGCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17kc</td>
 +
    <td>tRNA_heid_fw</td>
 +
    <td>53</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">GATTTCTGGAATTCGCGGCCGCTTCTAGAGTGTGCTTCTCAAATGCCTGAGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17kd</td>
 +
    <td>tRNA_heid_rev</td>
 +
    <td>55</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTACATGCAAAAAAGCCTGCTCGTTGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ke</td>
 +
    <td>BB1C3_tRNA_h_fw</td>
 +
    <td>51</td>
 +
    <td>62.0</td>
 +
    <td  style="font-size: 7.5pt;">GCCTGCTCAACGAGCAGGCTTTTTTGCATGTACTAGTAGCGGCCGCTGCAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17kf</td>
 +
    <td>BB1C3_tRNA_h_re</td>
 +
    <td>52</td>
 +
    <td>62.0</td>
 +
    <td  style="font-size: 7.5pt;">CAAACTGGCCTCAGGCATTTGAGAAGCACACTCTAGAAGCGGCCGCGAATTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17kg</td>
 +
    <td>pSC101-pK18msb_seqF</td>
 +
    <td>20</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">GTTCCACTGAGCGTCAGACC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17kh</td>
 +
    <td>pSC101-pK18msb_seqR</td>
 +
    <td>20</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">AACGCAGGAAAGAACATGTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ki</td>
 +
    <td>TyrRS_pSB1C3_fw</td>
 +
    <td>61</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GATTTTAGAGCCAATTAGAAAGAGATTATAACTGCAGTTTCAAACGCTAAATTGCCTGATG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17kj</td>
 +
    <td>TyrRS_pSB1C3_rev</td>
 +
    <td>63</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GTTATACGTTGTTTACGCTTTGAGGAATCCCATATGGACGAATTTGAAATGATAAAGAGAAAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17kk</td>
 +
    <td>PtNTT2_gaF_ext</td>
 +
    <td>62</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">CACTTTATGCTTCCGGCTCGTATAATGGACAAAGAGGAGAAAGTAATGCGCCCGTATCCGAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17kl</td>
 +
    <td>PtNTT2_gaR_ext</td>
 +
    <td>68</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">CTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTTTCTTTGTTGGTTTTCGCTTCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17km</td>
 +
    <td>LFS_Col</td>
 +
    <td>27</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">AATTTTCAGATGTGTTTTATCCGGGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17kn</td>
 +
    <td>RFS_Col</td>
 +
    <td>30</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">GTGATAAATGGTAATGTTGCTCATATCAGT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ko</td>
 +
    <td>PtNTT2_trunc_gaF_v2</td>
 +
    <td>46</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">TCGTATAATGGACAAAGAGGAGAAAGTAATGGGCGGCAGCACCGTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17kp</td>
 +
    <td>pSC101ori_ext_gaF</td>
 +
    <td>43</td>
 +
    <td>64.0</td>
 +
    <td  style="font-size: 7.5pt;">CCGTAGAAAAGATCAAAGGATCTTCAACGCCCTGAGAAGCCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17kq</td>
 +
    <td>pSC101ori_ext_gaR</td>
 +
    <td>47</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">CGTAAAAAGGCCGCGTTGCTGGCGTGCATCGCCTGTTTCAGGCTGTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17kr</td>
 +
    <td>K80+RBS+T7RNAPre</td>
 +
    <td>52</td>
 +
    <td>53.0</td>
 +
    <td  style="font-size: 7.5pt;">CGTCTACATTACTAGTTTCTCCTCTTTGTTAGCCCAAAAAACGGGTATGGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ks</td>
 +
    <td>K80T7RNAPp1</td>
 +
    <td>61</td>
 +
    <td>53.0</td>
 +
    <td  style="font-size: 7.5pt;">CCATACCCGTTTTTTGGGCTAACAAAGAGGAGAAACTAGTAATGTAGACGATTAACATCGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17kt</td>
 +
    <td>K808000+suff</td>
 +
    <td>51</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">CACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ku</td>
 +
    <td>K808000+suf_re</td>
 +
    <td>59</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">CCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17kv</td>
 +
    <td>K808000+Term</td>
 +
    <td>54</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">ATCTTAGAGTCGGACTTCGCGTTCGCGTAACCAGGCATCAAATAAAACGAAAGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17kw</td>
 +
    <td>K808000+Term_re</td>
 +
    <td>50</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">GACTGAGCCTTTCGTTTTATTTGATGCCTGGTTACGCGAACGCGAAGTCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17kx</td>
 +
    <td>Pre+K808000</td>
 +
    <td>54</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">GATTTCTGGAATTCGCGGCCGCTTCTAGAGTTATGACAACTTGACGGCTACATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ky</td>
 +
    <td>Pre+K808000_re</td>
 +
    <td>50</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGCCGTCAAGTTGTCATAACTCTAGAAGCGGCCGCGAATTCCAGAAATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17kz</td>
 +
    <td>T7RNAP_pos107_re</td>
 +
    <td>20</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">TTGCAGGAACTGGAAGGCTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17la</td>
 +
    <td>T7RNAP_pos107_BL</td>
 +
    <td>22</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">TTCCTGCAATAGATCAAGCCGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17lc</td>
 +
    <td>I746909+suff_rev</td>
 +
    <td>67</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">TTGCCGGACTGCAGCGGCCGCTACTAGTATAATACGACTCACTATAGGGAGATACTAGAGAAAGAGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ld</td>
 +
    <td>Pre+I746909re</td>
 +
    <td>52</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">GATTTCTGGAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGGCCCACC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17le</td>
 +
    <td>pre+I746909re_rev</td>
 +
    <td>55</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">GTGGGCCTTTCTGCGTTTATACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17lf</td>
 +
    <td>Seq_RFS</td>
 +
    <td>20</td>
 +
    <td>66.0</td>
 +
    <td  style="font-size: 7.5pt;">CGCCTGTGCCGCCCTTCAGA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17lg</td>
 +
    <td>pTarget_seq_fwd</td>
 +
    <td>20</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">GCCTTTGAGTGAGCTGATAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17lh</td>
 +
    <td>pTarget_seq_rev</td>
 +
    <td>20</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">CGGCACTGTTGCAAATAGTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17li</td>
 +
    <td>I746909+suff</td>
 +
    <td>49</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">TCTAGTATCTCCCTATAGTGAGTCGTATTATACTAGTAGCGGCCGCTGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17lj</td>
 +
    <td>PtNTT2_seq_fwd</td>
 +
    <td>23</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CCAGGAAGTGACCAAATTTCTGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17lk</td>
 +
    <td>PtNTT2_seq_rev</td>
 +
    <td>19</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">GCAGGCTCTGAATGGTGCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ll</td>
 +
    <td>pSC101-pK18msb-seq3</td>
 +
    <td>20</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">GAGGAGCAAACAGCGTTTGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17lm</td>
 +
    <td>pSC101-pK18msb-seq4</td>
 +
    <td>25</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">ATCTGTGCATATGGACAGTTTTCCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ln</td>
 +
    <td>sup35_pSB1C3_fw</td>
 +
    <td>69</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGATGTCTGACTCTAACCAGGGTAACAACCAGCAGAAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17lo</td>
 +
    <td>sup35_pSB1C3_rv</td>
 +
    <td>53</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">GCCGCTACTAGTATTAAACTTCGTCGTCAACTTCTTCTTCCTGTTCTTTGATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17lp</td>
 +
    <td>TyrFS_pSB1C3_fw</td>
 +
    <td>53</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">CTTTGAGGAATCCCATATGGACGAATTTGAAATGATAAAGAGAAACACATCTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17lq</td>
 +
    <td>TyrFS_pSB1C3_rv</td>
 +
    <td>67</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">GCGTTTGAAACTGCAGTTATAATCTCTTTCTAATTGGCTCTAAAATCTTTATAAGTTCTTCAGCTAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17lr</td>
 +
    <td>sgRNA_BB_A_1_gF</td>
 +
    <td>53</td>
 +
    <td>67.0</td>
 +
    <td  style="font-size: 7.5pt;">TTCTATAGTCCTTGAGAGGAGGAGTTACTAGTAGCGGCCGCTGCAGGAGTCAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ls</td>
 +
    <td>sgRNA_BB_G_2_gF</td>
 +
    <td>58</td>
 +
    <td>67.0</td>
 +
    <td  style="font-size: 7.5pt;">ATCAATACGTATACCAGGTGTCCTGTGAGCTACTAGTAGCGGCCGCTGCAGGAGTCAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17lt</td>
 +
    <td>sgRNA_BB_d_3_gF</td>
 +
    <td>58</td>
 +
    <td>67.0</td>
 +
    <td  style="font-size: 7.5pt;">CACGTATGAGAAGGTATTTGCCCGATAATCTACTAGTAGCGGCCGCTGCAGGAGTCAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17lu</td>
 +
    <td>sgRNA_BB_T_4_gF</td>
 +
    <td>58</td>
 +
    <td>67.0</td>
 +
    <td  style="font-size: 7.5pt;">AACTCCGGCAGGCAATTAAAGGGAACGTATTACTAGTAGCGGCCGCTGCAGGAGTCAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17lv</td>
 +
    <td>RomT_mutA_sense</td>
 +
    <td>80</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGAATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17lw</td>
 +
    <td>RomT_mutA_anti</td>
 +
    <td>80</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">AGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATTCTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17lx</td>
 +
    <td>RomT_mutG_sense</td>
 +
    <td>80</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGGATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ly</td>
 +
    <td>RomT_mutG_anti</td>
 +
    <td>80</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">AGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATCCTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17lz</td>
 +
    <td>RomT_mutD_sense</td>
 +
    <td>80</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCTT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ma</td>
 +
    <td>RomT_mutD_anti</td>
 +
    <td>80</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">AAGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATCTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mb</td>
 +
    <td>RomT_mutT_sense</td>
 +
    <td>80</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGTATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mc</td>
 +
    <td>RomT_mutT_anti</td>
 +
    <td>80</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">AGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATACTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17md</td>
 +
    <td>RomT_mutC_sense</td>
 +
    <td>80</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGCATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17me</td>
 +
    <td>RomT_mutC_anti</td>
 +
    <td>80</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">AGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATGCTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mf</td>
 +
    <td>ownT_mutA_sense</td>
 +
    <td>80</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CTAGCTCAGTCCTAGGTACAGTGCTAGCGATGATAGGCGGACTCTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mg</td>
 +
    <td>ownT_mutA_anti</td>
 +
    <td>80</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">GCCATCTAGTATTTCTCCTCTTTCACTAGTACCGAAGAGTCCGCCTATCATCGCTAGCACTGTACCTAGGACTGAGCTAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mh</td>
 +
    <td>ownT_mutG_sense</td>
 +
    <td>80</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CTAGCTCAGTCCTAGGTACAGTGCTAGCGATGATAGGCGGGCTCTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mi</td>
 +
    <td>ownT_mutG_anti</td>
 +
    <td>80</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">GCCATCTAGTATTTCTCCTCTTTCACTAGTACCGAAGAGCCCGCCTATCATCGCTAGCACTGTACCTAGGACTGAGCTAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mj</td>
 +
    <td>ownT_mutT_sense</td>
 +
    <td>80</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CTAGCTCAGTCCTAGGTACAGTGCTAGCGATGATAGGCGGTCTCTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mk</td>
 +
    <td>ownT_mutT_anti</td>
 +
    <td>80</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">GCCATCTAGTATTTCTCCTCTTTCACTAGTACCGAAGAGACCGCCTATCATCGCTAGCACTGTACCTAGGACTGAGCTAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ml</td>
 +
    <td>ownT_mutC_sense</td>
 +
    <td>80</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CTAGCTCAGTCCTAGGTACAGTGCTAGCGATGATAGGCGGCCTCTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mm</td>
 +
    <td>ownT_mutC_anti</td>
 +
    <td>80</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">GCCATCTAGTATTTCTCCTCTTTCACTAGTACCGAAGAGGCCGCCTATCATCGCTAGCACTGTACCTAGGACTGAGCTAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mn</td>
 +
    <td>pSC101-replace-fwd</td>
 +
    <td>45</td>
 +
    <td>71.0</td>
 +
    <td  style="font-size: 7.5pt;">TATGAAATTGGTGGTTGATAAGCGAGGCCGCCCGACTGATACGTT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mo</td>
 +
    <td>pSC101-replace-rev</td>
 +
    <td>55</td>
 +
    <td>64.0</td>
 +
    <td  style="font-size: 7.5pt;">GCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mp</td>
 +
    <td>pK18msB_split_1</td>
 +
    <td>29</td>
 +
    <td>66.0</td>
 +
    <td  style="font-size: 7.5pt;">GCAACTACAGCTCAGGCGACAACCATACG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mq</td>
 +
    <td>pK18msB_split_2</td>
 +
    <td>29</td>
 +
    <td>66.0</td>
 +
    <td  style="font-size: 7.5pt;">CGTATGGTTGTCGCCTGAGCTGTAGTTGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mr</td>
 +
    <td>sup_21TGT_fw</td>
 +
    <td>50</td>
 +
    <td>62.0</td>
 +
    <td  style="font-size: 7.5pt;">CCAGCAGTACTCTCAGAATGGATGTCAACAGCAAGGCAATAATCGTTATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ms</td>
 +
    <td>sup_21TGT_rev</td>
 +
    <td>70</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GTTGACATCCATTCTGAGAGTACTGCTGGTAATTCTGCTGGTTGTTACCCTGGTTAGAGTCAGACATCTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mt</td>
 +
    <td>TyrRS_FluoAS_fw</td>
 +
    <td>67</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GATTAAAATCCTGGAGCCCATTCGCAAGCGCCTGTAACTGCAGTTTCAAACGCTAAATTGCCTGATG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mu</td>
 +
    <td>TyrRS_FluoAS_rv</td>
 +
    <td>70</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GATGTGTTACGCTTAATCATCTCGAACTCGTCCATATGGGATTCCTCAAAGCGTAAACAACGTATAACGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mv</td>
 +
    <td>ONBY-out-rev_2</td>
 +
    <td>35</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">ATGAATCTCCAAGTATATAACGACTGCACCTTTGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mw</td>
 +
    <td>CLRS-fw</td>
 +
    <td>40</td>
 +
    <td>73.0</td>
 +
    <td  style="font-size: 7.5pt;">GAATTCGCGGCCGCTTCTAGAGTTTTAAAAAACTAACAGT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mx</td>
 +
    <td>CLRS-rv</td>
 +
    <td>40</td>
 +
    <td>80.0</td>
 +
    <td  style="font-size: 7.5pt;">CTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17my</td>
 +
    <td>PrK_210CTT_fw</td>
 +
    <td>41</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">CCGAAAGACGAAATCAGCCTTAATTCCGGCAAACCGTTTCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17mz</td>
 +
    <td>PrK_210CTT_rv</td>
 +
    <td>39</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">AAGGCTGATTTCGTCTTTCGGATTCAGCAGAACCTCCAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17na</td>
 +
    <td>PrK_456CTT_fw</td>
 +
    <td>46</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">CTTTAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17nb</td>
 +
    <td>PrK_456CTT_rv</td>
 +
    <td>54</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">CAATTTAGCGTTTGAAACTAAAGTTACAGGTTCGTAGAGATCCCGTTATAATAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17nc</td>
 +
    <td>AcF_308CTT_fw</td>
 +
    <td>46</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">CTTCAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17nd</td>
 +
    <td>AcF_308CTT_rv</td>
 +
    <td>54</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">CAATTTAGCGTTTGAAACTGAAGTTATAATCTCTTTCTAATTGGCTCTAAAATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ne</td>
 +
    <td>AcF_107GAG_fw</td>
 +
    <td>59</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">AGTGAGTTCCAGCTTGATAAGGATTATACACTGAATGTCTATAGATTGGCTTTAAAAAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17nf</td>
 +
    <td>AcF_107GAG_rv</td>
 +
    <td>56</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">GTGTATAATCCTTATCAAGCTGGAACTCACTTCCATAAACATATTTTGCCTTTAAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ng</td>
 +
    <td>sup35_121CTA_fw</td>
 +
    <td>51</td>
 +
    <td>70.0</td>
 +
    <td  style="font-size: 7.5pt;">CAAGCTGGATTCCAGCCTCAACTACAGGGGATGTCGCTTAATGACTTTCAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17nh</td>
 +
    <td>sup35_121CTA_rv</td>
 +
    <td>49</td>
 +
    <td>70.0</td>
 +
    <td  style="font-size: 7.5pt;">CCCCTGTAGTTGAGGCTGGAATCCAGCTTGATAACCTTGCAAATTGTTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ni</td>
 +
    <td>sup35_Histag_rev</td>
 +
    <td>73</td>
 +
    <td>70.0</td>
 +
    <td  style="font-size: 7.5pt;">TTAATGGTGATGATGGTGGTGATGGTGATGATGAACTTCGTCGTCAACTTCTTCTTCCTGTTCTTTAATTAAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17nj</td>
 +
    <td>blocking_L_fw</td>
 +
    <td>42</td>
 +
    <td>72.0</td>
 +
    <td  style="font-size: 7.5pt;">CGGCCTGCTGACTTTCTCGCCGATCAAAAGGCATTTTGCTAT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17nk</td>
 +
    <td>blocking_L_rv</td>
 +
    <td>37</td>
 +
    <td>72.0</td>
 +
    <td  style="font-size: 7.5pt;">GCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17nl</td>
 +
    <td>blocking_R_fw</td>
 +
    <td>72</td>
 +
    <td>70.0</td>
 +
    <td  style="font-size: 7.5pt;">GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17nm</td>
 +
    <td>blocking_R_rv</td>
 +
    <td>48</td>
 +
    <td>70.0</td>
 +
    <td  style="font-size: 7.5pt;">AAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTAAAAAAAGCACCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17nn</td>
 +
    <td>sgRNA_biobrck_F</td>
 +
    <td>44</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">CTAGCATCTAGTGACTCTAGAGCTAAGCGGCCTGCTGACTTTCT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17no</td>
 +
    <td>sgRNA_biobrck_R</td>
 +
    <td>59</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GAACTAGACAGATTCTGCAGCGGCCGCTACTAGTAAAAAAAGCCTGCTCGTTGAGCAGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17np</td>
 +
    <td>FluoAS_308CTT</td>
 +
    <td>67</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">CATCAGGCAATTTAGCGTTTGAAACTGAAGTTACAGGCGCTTGCGAATGGGCTCCAGGATTTTAATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17nq</td>
 +
    <td>Prüf_ori_fw_58</td>
 +
    <td>32</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CCTTTTTCTTTAAAACCGAAAAGATTACTTCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17nr</td>
 +
    <td>Prüf_ori_rev_58</td>
 +
    <td>22</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CGGGGCGTAATTTGATATCGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ns</td>
 +
    <td>NPA_seq_fwd</td>
 +
    <td>19</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">CCATTTGGGTGATCTTGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17nt</td>
 +
    <td>NPA_seq_rev</td>
 +
    <td>24</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">CAAGGATTCTAACTCCTCATACGA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17nu</td>
 +
    <td>GFP_tag_gaF</td>
 +
    <td>55</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">GAACAAAAGCTTATTTCTGAAGAGGACTTGCGTAAAGGAGAAGAACTTTTCACTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17nv</td>
 +
    <td>GFP_tag_gaR</td>
 +
    <td>57</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTGTATAGTTCATCCATGCCATG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17nw</td>
 +
    <td>PtNTT2_tag_gaR</td>
 +
    <td>55</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">CAAGTCCTCTTCAGAAATAAGCTTTTGTTCTTTTTCTTTGTTGGTTTTCGCTTCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17nx</td>
 +
    <td>KanR_fw</td>
 +
    <td>54</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGATGAGCCATATTCAACGGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ny</td>
 +
    <td>KanR_rev</td>
 +
    <td>49</td>
 +
    <td>53.0</td>
 +
    <td  style="font-size: 7.5pt;">GCCGGACTGCAGCGGCCGCTACTAGTATTAGAAAAACTCATCGAGCATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17nz</td>
 +
    <td>KanR_S133Am</td>
 +
    <td>23</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GCGCCGGTTGCATTAGATTCCTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17oa</td>
 +
    <td>KanR_S133Am_rev</td>
 +
    <td>19</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GCGCAGGAACACTGCCAGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ob</td>
 +
    <td>KanR_S154Am</td>
 +
    <td>26</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">CAGGCGCAATAGCGAATGAATAACGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17oc</td>
 +
    <td>KanR_S154Am_rev</td>
 +
    <td>23</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">GCCTGAGCGAGACGAAATACGCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17od</td>
 +
    <td>pSB1C3_PF_rev</td>
 +
    <td>52</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">CTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATCTCTAGAAGCGGCCGCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17oe</td>
 +
    <td>pSB1C3_SF_fw</td>
 +
    <td>54</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">TGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATACTAGTAGCGGCCGCTGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17of</td>
 +
    <td>T7RNAP_BL_fw</td>
 +
    <td>20</td>
 +
    <td>62.0</td>
 +
    <td  style="font-size: 7.5pt;">CAGACATTGCCGTCACTGCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17og</td>
 +
    <td>T7RNAP_BL_rev</td>
 +
    <td>17</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">GCCACGAAGACCACGCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17oh</td>
 +
    <td>T7_CBS_fw</td>
 +
    <td>63</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GACTCACTATAGGGAATACAAGCTACTTGTTCTTTTTGCATACTAGTAGCGGCCGCTGCAGTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17oi</td>
 +
    <td>T7_CBS_rev</td>
 +
    <td>72</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">CAAGTAGCTTGTATTCCCTATAGTGAGTCGTATTACTCTAGTATTAGCTATTCAGATTTGCGATACACCACG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17oj</td>
 +
    <td>KanR_rev_fw</td>
 +
    <td>56</td>
 +
    <td>52.0</td>
 +
    <td  style="font-size: 7.5pt;">GGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTAGAAAAACTCATCGAGCATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ok</td>
 +
    <td>KanR_rev_rev</td>
 +
    <td>54</td>
 +
    <td>52.0</td>
 +
    <td  style="font-size: 7.5pt;">CCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAATGAGCCATATTCAACGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ol</td>
 +
    <td>mutT_flanke1_gaF</td>
 +
    <td>50</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TAATGTGAGTTAGCTCACTCATTAGGCACCCTCGATTTGCCAATTGCCGA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17om</td>
 +
    <td>mutT_flanke1_gaR</td>
 +
    <td>55</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">GTCGGATGCGGCGAAAACGCCTTATCTGACTGTCTTAAACCTATAAAAAAGGCGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17on</td>
 +
    <td>mutT-flanke2_gaF</td>
 +
    <td>50</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">ATTCTGCGCCTTTTTTATAGGTTTAAGACAGTCAGATAAGGCGTTTTCGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17oo</td>
 +
    <td>mutT-flanke2-gaR</td>
 +
    <td>49</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">CTGCGGACTGGCTTTCTACGTGTTCCGCTTGGCACTTACCATGGTGCTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17op</td>
 +
    <td>Cas9only_F</td>
 +
    <td>64</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">GATTTCTGGAATTCGCGGCCGCTTCTAGAGATGGATAAGAAATACTCAATAGGCTTAGATATCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17oq</td>
 +
    <td>Cas9only_R</td>
 +
    <td>51</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGTCACCTCCTAGCTGACT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17or</td>
 +
    <td>bb_Cas9only_F</td>
 +
    <td>55</td>
 +
    <td>67.0</td>
 +
    <td  style="font-size: 7.5pt;">ATTGATTTGAGTCAGCTAGGAGGTGACTGATACTAGTAGCGGCCGCTGCAGTCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17os</td>
 +
    <td>bb_Cas9only_R</td>
 +
    <td>60</td>
 +
    <td>66.0</td>
 +
    <td  style="font-size: 7.5pt;">ATCTAAGCCTATTGAGTATTTCTTATCCATCTCTAGAAGCGGCCGCGAATTCCAGAAATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ot</td>
 +
    <td>crtI_86TAG_fw</td>
 +
    <td>51</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">GAACTGCTGCCGGTTACGCCGTAGTACCGCCTGTGTTGGGAGTCAGGGAAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ou</td>
 +
    <td>crtI_86TAG_rev</td>
 +
    <td>64</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">ACGGCGTAACCGGCAGCAGTTCGACATACTCTTTTAACTGTTTTCCTGCCAGTGCAAACAGTTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ov</td>
 +
    <td>crtI_318TAG_fw</td>
 +
    <td>55</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">CTATTTTGGTTTGAATCACCATCATTAGCAGCTCGCGCATCACACGGTTTGTTTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ow</td>
 +
    <td>crtI_318TAG_rev</td>
 +
    <td>61</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">ATGATGGTGATTCAAACCAAAATAGAGCACAAACAGAGAGTTACTCATGCGCTTAGTTTGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ox</td>
 +
    <td>crtI_353TAG_fw</td>
 +
    <td>49</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">GAGGACTTCTCACTTTATCTGTAGGCGCCCTGTGTCACGGATTCGTCAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17oy</td>
 +
    <td>crtI_353TAG_rev</td>
 +
    <td>60</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">CAGATAAAGTGAGAAGTCCTCTGCGAGGCCATCATGATTAAAAATTTCGTCAATCAGCTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17oz</td>
 +
    <td>BB_ovexGA_F</td>
 +
    <td>18</td>
 +
    <td>52.0</td>
 +
    <td  style="font-size: 7.5pt;">GATTTCTGGAATTCGCGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pa</td>
 +
    <td>BB_ovexGA_R</td>
 +
    <td>15</td>
 +
    <td>52.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pb</td>
 +
    <td>B0015_ovex_F</td>
 +
    <td>54</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">ATTGATTTGAGTCAGCTAGGAGGTGACTGACCAGGCATCAAATAAAACGAAAGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pc</td>
 +
    <td>B0015_ovex_R</td>
 +
    <td>51</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">AATGGTAATGTTGCTCATATCAGTATCTCATATAAACGCAGAAAGGCCCAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pd</td>
 +
    <td>B0015_GA_F</td>
 +
    <td>54</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">GATTTCTGGAATTCGCGGCCGCTTCTAGAGCCAGGCATCAAATAAAACGAAAGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pe</td>
 +
    <td>B0015_GA_R</td>
 +
    <td>51</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pf</td>
 +
    <td>Cas9o_ovex_F</td>
 +
    <td>68</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">TGTGAGCGGATAACAATTTCACACAGGAATACTAGATGGATAAGAAATACTCAATAGGCTTAGATATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pg</td>
 +
    <td>Cas9o_ovex_R</td>
 +
    <td>50</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">ACTGAGCCTTTCGTTTTATTTGATGCCTGGGTCAGCTAGGAGGTGACTGA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ph</td>
 +
    <td>RFS_ovex_F</td>
 +
    <td>57</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">CACCTTCGGGTGGGCCTTTCTGCGTTTATATGAGATACTGATATGAGCAACATTACC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pi</td>
 +
    <td>RFS_GA57_R</td>
 +
    <td>60</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTGCCGGACTGCAGCGGCCGCTACTAGTACAATAAATGTAATGCGTTATATCTTCCTGA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pj</td>
 +
    <td>Seq_crtE1_fw</td>
 +
    <td>22</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CTACGAGGATCAGGGGTTTACC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pk</td>
 +
    <td>Seq_crtE2_fw</td>
 +
    <td>22</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">GTGGTTCATACCTATCGCGACC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pl</td>
 +
    <td>Cas9o_ovex_R2</td>
 +
    <td>50</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">ACTGAGCCTTTCGTTTTATTTGATGCCTGGTCAGTCACCTCCTAGCTGAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pm</td>
 +
    <td>28567c3g1_gF</td>
 +
    <td>64</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCCTTGGATCCTAAAGTGAAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pn</td>
 +
    <td>28567c3g1_gR</td>
 +
    <td>61</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCATTCCCATTCGATGGTTGATGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17po</td>
 +
    <td>27205c6g2i2_gF</td>
 +
    <td>63</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCTTTAGAATTGCAAGACGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pp</td>
 +
    <td>27205c6g2i2_gR</td>
 +
    <td>67</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAGAAGGCCTTTTTCTCATAAGAAATGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pq</td>
 +
    <td>24513c0g1_gF</td>
 +
    <td>65</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCTTCTTCAGCTTCCAATAATG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pr</td>
 +
    <td>24513c0g1i3_gR</td>
 +
    <td>73</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAAGAGAAAAGTCGTTTTTCATATGAGTATAAACC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ps</td>
 +
    <td>24513c0g1i1_gR</td>
 +
    <td>73</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAAGAATAGAGACGTTTTTCATATGAGTATAAACC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pt</td>
 +
    <td>27205c6g2i4_gR</td>
 +
    <td>63</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCATGTAGGGAACATCCAGAAGCTTA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pu</td>
 +
    <td>107595c0g1i1_gF</td>
 +
    <td>64</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGAACCTCAATTCTCTCCGCCTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pv</td>
 +
    <td>107595c0g1i1_gR</td>
 +
    <td>64</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCACTTGTATATCAAGGCATCTCGTCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pw</td>
 +
    <td>30924c1g1i14_gF</td>
 +
    <td>63</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGGGTCGTTGAAAAATGAAGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17px</td>
 +
    <td>30924c1g1i14_gR</td>
 +
    <td>64</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAAACACTAAGCTTGGGATGGTAATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17py</td>
 +
    <td>30983c0g1i12_gF</td>
 +
    <td>65</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGTGTTCCAAAAAATAGCAGCCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17pz</td>
 +
    <td>30983c0g1i12_gR</td>
 +
    <td>57</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCACTTGCCGCCCGTTTTCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qa</td>
 +
    <td>26152c4g1i4_gF</td>
 +
    <td>75</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAAAACGAAAACAAAATAGAAAGTAACAGTAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qb</td>
 +
    <td>26152c4g1i4_gR</td>
 +
    <td>68</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCATTTACCATCCATTTTCCACTGTAAGTAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qc</td>
 +
    <td>29270c2g2i7_gF</td>
 +
    <td>64</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAAGGTCAATATCCACAGCAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qd</td>
 +
    <td>29270c2g2i7_gR</td>
 +
    <td>67</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCACACAAGCTGCACAACAGCAGCCACTCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qe</td>
 +
    <td>30924c1g1i5_gR</td>
 +
    <td>60</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAAAGCAGTTCCATCCGTGTAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qf</td>
 +
    <td>26152c4g1i8_gF</td>
 +
    <td>63</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAGAATCAAACTGCTCTTGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qg</td>
 +
    <td>L_rob_pSB1C3_fw_65</td>
 +
    <td>52</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTGGTCCTGGCCACGGGTGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qh</td>
 +
    <td>L_pSB1C3_rop_rev_65</td>
 +
    <td>49</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">GCCGCGTTGCTGGCGTTGGTCCTGGCCACGGGTGCGCATGATCGTGCTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qi</td>
 +
    <td>k_rop_pSB1C3_fw_66</td>
 +
    <td>53</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTGCGGAAGTCAGCGCCCTGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qj</td>
 +
    <td>k_pSB1C3_rop_rev_65</td>
 +
    <td>50</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">CGGAACATAATGGTGCAGGGCGCTGACTTCCGCAACGCCAGCAACGCGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qk</td>
 +
    <td>Cas9_EcoRI_F</td>
 +
    <td>55</td>
 +
    <td>67.0</td>
 +
    <td  style="font-size: 7.5pt;">AGACAATCGTGAGAAGATTGAAAAAATCTTGACTTTTCGCATCCCTTATTATGTT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ql</td>
 +
    <td>Cas9_EcoRI_R</td>
 +
    <td>50</td>
 +
    <td>73.0</td>
 +
    <td  style="font-size: 7.5pt;">TTGCCACGCGCCAATGGACCAACATAATAAGGGATGCGAAAAGTCAAGAT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qm</td>
 +
    <td>28626c0g1i1_gF</td>
 +
    <td>65</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAGCAGTACAGATTCGTAATCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qn</td>
 +
    <td>28626c0g1i1_gR</td>
 +
    <td>65</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAACAGGACTTATTAGAACTGCAATGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qo</td>
 +
    <td>CLRS-fw_OL</td>
 +
    <td>50</td>
 +
    <td>77.0</td>
 +
    <td  style="font-size: 7.5pt;">TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTTTAAAA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qp</td>
 +
    <td>CLRS-rv_OL</td>
 +
    <td>50</td>
 +
    <td>83.0</td>
 +
    <td  style="font-size: 7.5pt;">CCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATATAAACGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qq</td>
 +
    <td>gRNA_fw_codA</td>
 +
    <td>25</td>
 +
    <td>0.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGC CACAGTGAACGTCGATGAGA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qr</td>
 +
    <td>gRNA_rv_codA</td>
 +
    <td>25</td>
 +
    <td>0.0</td>
 +
    <td  style="font-size: 7.5pt;">AAAC TCTCATCGACGTTCACTGTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qs</td>
 +
    <td>pSB3K5-UBP-gaF</td>
 +
    <td>19</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">GCCGCTGCAGGAGTCACTA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qt</td>
 +
    <td>pSB3K5-UBP-gaR</td>
 +
    <td>22</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">CCGCGAATTCGAGTCACTAAGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qu</td>
 +
    <td>mRFP-UBP-gaF</td>
 +
    <td>22</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">CCCTTAGTGACTCGAATTCGCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qv</td>
 +
    <td>mRFP-Link-ccdB-gaR</td>
 +
    <td>43</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">AAACCTTAAACTGTGCGGCTGCCGCAGCACCGGTGGAGTGACG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qw</td>
 +
    <td>ccdB-Linker-mRFP-gaF</td>
 +
    <td>58</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">CTCCACCGGTGCTGCGGCAGCCGCACAGTTTAAGGTTTACACCTATAAAAGAGAGAGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qx</td>
 +
    <td>ccdB-UBP_gaR</td>
 +
    <td>60</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGTGACTCCTGCAGCGGCCGCTACTAGTATTATATTCCCCAGAACATCAGGTTAATGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qy</td>
 +
    <td>ccdB-UBP_gaF</td>
 +
    <td>63</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGTGAAAGAGGAGAAATACTAGATGGCTTCAGTTTAAGGTTTACACCTATAAAAGAGAGAGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17qz</td>
 +
    <td>mRFP-UBP_gaR</td>
 +
    <td>47</td>
 +
    <td>62.0</td>
 +
    <td  style="font-size: 7.5pt;">GTGGCTAGCACTGTACCTAGGACTGAGCTAGCACCGGTGGAGTGACG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ra</td>
 +
    <td>PtNTT2_bb_gaR</td>
 +
    <td>32</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">CATTACTTTCTCCTCTTTGTCCATTATACGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rb</td>
 +
    <td>PtNTT2-noSP_gaF</td>
 +
    <td>52</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">CTCGTATAATGGACAAAGAGGAGAAAGTAATGCTGCCGGTGAAAAAAGGCAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rc</td>
 +
    <td>CLtRNA_fw</td>
 +
    <td>47</td>
 +
    <td>72.0</td>
 +
    <td  style="font-size: 7.5pt;">TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTGTGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rd</td>
 +
    <td>CLtRNA_rv</td>
 +
    <td>30</td>
 +
    <td>72.0</td>
 +
    <td  style="font-size: 7.5pt;">GCCGCTGCAGTCCGGCAAAAAAGGGCAAGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17re</td>
 +
    <td>RDL_SELP_fw</td>
 +
    <td>50</td>
 +
    <td>72.0</td>
 +
    <td  style="font-size: 7.5pt;">TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAGAGGAGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rf</td>
 +
    <td>RDL_SELP_rv</td>
 +
    <td>30</td>
 +
    <td>72.0</td>
 +
    <td  style="font-size: 7.5pt;">CTTGCCCTTTTTTGCCGGACTGCAGCGGCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rg</td>
 +
    <td>XbaI_RBS_baBL_fw</td>
 +
    <td>51</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">CTTCTAGAGAAAGAGGAGAAACTAGTAATGGCAGCATAGGTTATCAACACG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rh</td>
 +
    <td>SpeI_baBL_rev</td>
 +
    <td>45</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">GCTACTAGTATTATCTGATTTTTGTAAAGGTCTGATAATGGTCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ri</td>
 +
    <td>Rubisco_110ATT_fw</td>
 +
    <td>46</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">CGCGGCAATTAGTAAGATATCAAAGAGGAGAAATACTAGATGGCAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rj</td>
 +
    <td>Rubisco_110ATT_rv</td>
 +
    <td>41</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">GATATCTTACTAATTGCCGCGGTAGACCACGAAGGCCAGAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rk</td>
 +
    <td>BcuI_R</td>
 +
    <td>40</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">TCCTCTTTCACAAGAAGCTAGCACTGTACCTAGGACTGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rl</td>
 +
    <td>BcuI_F</td>
 +
    <td>40</td>
 +
    <td>66.0</td>
 +
    <td  style="font-size: 7.5pt;">TGCTAGCTTCTTGTGAAAGAGGAGAAATACTAGATGGCTT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rm</td>
 +
    <td>MHA_GA_F</td>
 +
    <td>60</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">ATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rn</td>
 +
    <td>MHA_GA_R</td>
 +
    <td>70</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">CGTATAACGTTACTGGTTTCATCTAGTATTTCTCCTCTTTCACAAGAAGCTAGCACTGTACCTAGGACTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ro</td>
 +
    <td>lacI_BB_R</td>
 +
    <td>62</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">TACCTAGGACTGAGCTAGCCGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rp</td>
 +
    <td>lacI_BB_F</td>
 +
    <td>67</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">TCTTGTGAAAGAGGAGAAATACTAGATGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rq</td>
 +
    <td>fwd_CDD_pSB1C3</td>
 +
    <td>56</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGATGCAAGCCAAACCATTTCAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rr</td>
 +
    <td>rev_CDD_pSB1C3</td>
 +
    <td>71</td>
 +
    <td>62.0</td>
 +
    <td  style="font-size: 7.5pt;">CCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATCAATTGAAATTTAAATCTAATGTATCAATTGAATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rs</td>
 +
    <td>Split_RBS_F</td>
 +
    <td>45</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">TACTAGTGAAAGAGGAGAAATACTAGAGATGGCTTCCTCCGAAGA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rt</td>
 +
    <td>Split_RBS_R</td>
 +
    <td>46</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TTTCTCCTCTTTCACTAGTATGTGTGAAATTGTTATCCGCTCACAA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ru</td>
 +
    <td>PtNTT2-pelB-SP_gaF</td>
 +
    <td>65</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">GCGGCGGCGGGCCTGCTGCTGCTGGCGGCGCAGCCGGCGATGGCGCTGCCGGTGAAAAAAGGCAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rv</td>
 +
    <td>PtNTT2_bb_pelB-SP_gaR</td>
 +
    <td>74</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">CAGCAGCAGCAGGCCCGCCGCCGCGGTCGGCAGCAGATATTTCATTACTTTCTCCTCTTTGTCCATTATACGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rw</td>
 +
    <td>PtNTT2_TAT-SP_gaF</td>
 +
    <td>77</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">ACCACAGGACTTGCCACTATCGGCGCTGCCAGCATGTTTATGCCAAAGGCCAACGCCCTGCCGGTGAAAAAAGGCAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rx</td>
 +
    <td>PtNTT2_bb_TAT-SP_gaR</td>
 +
    <td>86</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">GCCGATAGTGGCAAGTCCTGTGGTGGCTTTTAAGAAGCCTCGGCGGTTTATTTGCATTACTTTCTCCTCTTTGTCCATTATACGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ry</td>
 +
    <td>PtNTT2-only_gaF</td>
 +
    <td>43</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">CTGGAATTCGCGGCCGCTTCTAGAGATGCGCCCGTATCCGACC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17rz</td>
 +
    <td>fwd_CDD_SpeI</td>
 +
    <td>50</td>
 +
    <td>50.0</td>
 +
    <td  style="font-size: 7.5pt;">TGATGAAAAATGTGGCTAATACCAGTGTTTTGAAATGGGGTGGAATATTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sa</td>
 +
    <td>rev_CDD_SpeI</td>
 +
    <td>76</td>
 +
    <td>76.0</td>
 +
    <td  style="font-size: 7.5pt;">AATATTCCACCCCATTTCAAAACACTGGTATTAGCCACATTTTTCATCACCTTGGTATTTCCAAATTTCTTACCTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sb</td>
 +
    <td>AcF_107GAG2_fw</td>
 +
    <td>62</td>
 +
    <td>64.0</td>
 +
    <td  style="font-size: 7.5pt;">GATAAGGATTATACACTGAATGTCTATAGATTGGCTTTAAAAACTACCTTAAAAAGAGCAAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sc</td>
 +
    <td>AcF_107GAG2_rv</td>
 +
    <td>48</td>
 +
    <td>64.0</td>
 +
    <td  style="font-size: 7.5pt;">GACATTCAGTGTATAATCCTTATCAAGCTGGAACTCACTTCCATAAAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sd</td>
 +
    <td>PtNTT2-lacO-gaR</td>
 +
    <td>53</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">TCGACTGAGCCTTTCGTTTTATTTGTTATTTTTCTTTGTTGGTTTTCGCTTCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17se</td>
 +
    <td>lacO-PtNTT2_gaF</td>
 +
    <td>50</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">AAGCGAAAACCAACAAAGAAAAATAACAAATAAAACGAAAGGCTCAGTCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sf</td>
 +
    <td>lacO-PtNTT2_gaR</td>
 +
    <td>54</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">ATCTAAGCCTATTGAGTATTTCTTATCCATTTCCTGTGTGAAATTGTTATCCGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sg</td>
 +
    <td>Cas9_gaF</td>
 +
    <td>63</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">TTGTGAGCGGATAACAATTTCACACAGGAAATGGATAAGAAATACTCAATAGGCTTAGATATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sh</td>
 +
    <td>is_kan_fw</td>
 +
    <td>44</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">CCAATTAACCAATTCTGAATGATTGAACAAGATGGATTGCACGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17si</td>
 +
    <td>is_kan_rev</td>
 +
    <td>53</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">CATAAACAGTAATACAAGGGGTGTTTCAGAAGAACTCGTCAAGAAGGCGATAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sj</td>
 +
    <td>bb_kan_fv</td>
 +
    <td>56</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">CGCCTTCTTGACGAGTTCTTCTGAAACACCCCTTGTATTACTGTTTATGTAAGCAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sk</td>
 +
    <td>bb_kan_rv</td>
 +
    <td>53</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">GCAATCCATCTTGTTCAATCATTCAGAATTGGTTAATTGGTTGTAACACTGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sl</td>
 +
    <td>pSB_CFP_fw</td>
 +
    <td>54</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">GTCACTCCACCGGTGCTTAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sm</td>
 +
    <td>pSB_YFP_rev</td>
 +
    <td>60</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">CAGTTCTTCGCCTTTGCTCATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sn</td>
 +
    <td>CFP_YFP_fw</td>
 +
    <td>50</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GAATTCGCGGCCGCTTCTAGAGATGAGCAAAGGCGAAGAACTGTTTACCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17so</td>
 +
    <td>CFP_pSB_rev</td>
 +
    <td>64</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GAACCAGCAGCGGAGCCAGCGGATCCCATTTTATACAGTTCATCCATGCCATGGGTAATACCGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sp</td>
 +
    <td>YFP_CFP_fw</td>
 +
    <td>67</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GGGATCCGCTGGCTCCGCTGCTGGTTCTGGCGAATAGATGGCTTCCTCCGAAGACGTTATCAAAGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sq</td>
 +
    <td>YFP_pSB_rev</td>
 +
    <td>53</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">CCGGACTGCAGCGGCCGCTACTAGTATTAAGCACCGGTGGAGTGACGACCTTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sr</td>
 +
    <td>fwd_CCD_Topo</td>
 +
    <td>25</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">CCGTTTCTCATCGAATATGCAAGCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ss</td>
 +
    <td>rev_CCD_Topo</td>
 +
    <td>26</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">GCCCAAAAACCATGAAATCCAAGTGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17st</td>
 +
    <td>fwd_pTXB1_backb</td>
 +
    <td>21</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">TGCATCACGGGAGATGCACTA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17su</td>
 +
    <td>rev_pTXB1_backb</td>
 +
    <td>45</td>
 +
    <td>66.0</td>
 +
    <td  style="font-size: 7.5pt;">ATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sv</td>
 +
    <td>pC3_YFP_fw</td>
 +
    <td>49</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">GATGAACTGTATAAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sw</td>
 +
    <td>pC3_CFP_rv</td>
 +
    <td>51</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">GGAGGAAGCCATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sx</td>
 +
    <td>YFP_pC3_fw</td>
 +
    <td>60</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GATTTCTGGAATTCGCGGCCGCTTCTAGAGATGGCTTCCTCCGAAGACGTTATCAAAGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sy</td>
 +
    <td>YFP_CFP_rv</td>
 +
    <td>52</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">CAGAACCAGCAGCGGAGCCAGCGGATCCCATAGCACCGGTGGAGTGACGACC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17sz</td>
 +
    <td>CFP_YFP_fw</td>
 +
    <td>56</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">TGGCTCCGCTGCTGGTTCTGGCGAATAGATGAGCAAAGGCGAAGAACTGTTTACCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ta</td>
 +
    <td>CFP_pC3_rev</td>
 +
    <td>58</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GGACTGCAGCGGCCGCTACTAGTATTTATACAGTTCATCCATGCCATGGGTAATACCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17tb</td>
 +
    <td>B0015_ba_fw    </td>
 +
    <td>58</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">CCATTATCAGACCTTTACAAAAATCAGATAACCAGGCATCAAATAAAACGAAAGGCTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17tc</td>
 +
    <td>B0015_ba_r_rev  </td>
 +
    <td>64</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">CGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGGCCCACCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17td</td>
 +
    <td>baBL_fw        </td>
 +
    <td>67</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">CTCCATACCCGTTTTTTGGGCTAACAAAGAGGAGAAACTACTAATGGCAGCATAGGTTATCAACACG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17te</td>
 +
    <td>baBL_rev        </td>
 +
    <td>70</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">CGACTGAGCCTTTCGTTTTATTTGATGCCTGGTTATCTGATTTTTGTAAAGGTCTGATAATGGTCCGTTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17tf</td>
 +
    <td>K80_ba_rev      </td>
 +
    <td>70</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">GATAACCTATGCTGCCATTAGTAGTTTCTCCTCTTTGTTAGCCCAAAAAACGGGTATGGAGAAACAGTAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17tg</td>
 +
    <td>K808000_ba_r_rev</td>
 +
    <td>60</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">CCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATGACAACTTGACGGCTACATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17th</td>
 +
    <td>pSB1C3_ba_r_fw  </td>
 +
    <td>66</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATACTAGTAGCGGCCGCTGCAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ti</td>
 +
    <td>pSB1C3_ba_r_rev </td>
 +
    <td>64</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATACTCTAGAAGCGGCCGCGAATTCCAGAAATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17tj</td>
 +
    <td>pSB1C3_prom_fw </td>
 +
    <td>69</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">CCTCTTTGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAATACTAGTAGCGGCCGCTGCAGTCCGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17tk</td>
 +
    <td>Term_kanR_rev  </td>
 +
    <td>69</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">CCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGGCCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17tl</td>
 +
    <td>Term_kanR_fw  </td>
 +
    <td>53</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">GCAGTTTCATTTGATGCTCGATGAGTTTTTCTAACCAGGCATCAAATAAAACG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17tm</td>
 +
    <td>pSB1C3_term_rev</td>
 +
    <td>70</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">CTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17tn</td>
 +
    <td>kanR_term_rev  </td>
 +
    <td>71</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">GGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGTTAGAAAAACTCATCGAGCATCAAATG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17to</td>
 +
    <td>kanR_prom_fw  </td>
 +
    <td>70</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">GACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCAAAGAGGAGAAAGTAGTAATGAGCCATATTCAACGGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17tp</td>
 +
    <td>SpeI_weg_fw              </td>
 +
    <td>24</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">GAGGAGAAAGTAGTAATGTAGACG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17tq</td>
 +
    <td>SpeI_weg_rev              </td>
 +
    <td>21</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">CTTTCTCCTCTTTGTTAGCCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17tr</td>
 +
    <td>qPCR_mRFP_F</td>
 +
    <td>23</td>
 +
    <td>62.0</td>
 +
    <td  style="font-size: 7.5pt;">AAGGTCGTCCGTACGAAGGTACC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ts</td>
 +
    <td>qPCR_mRFP_R</td>
 +
    <td>25</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">TCAGCCGGGTGTTTAACGTAAGCTT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17tt</td>
 +
    <td>Cm_pSB6A1_fw_53</td>
 +
    <td>42</td>
 +
    <td>53.0</td>
 +
    <td  style="font-size: 7.5pt;">GAGTAAACTTGGTCTGACAGGATTCTCACCAATAAAAAACGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17tu</td>
 +
    <td>Cm_pSB6A1_rev_53</td>
 +
    <td>46</td>
 +
    <td>53.0</td>
 +
    <td  style="font-size: 7.5pt;">GTTAATGTCATGATAATAATGGTTTCTTAGACCGTCAGGTGGCACT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17tv</td>
 +
    <td>pSB6A1_Cm_fw_52</td>
 +
    <td>53</td>
 +
    <td>52.0</td>
 +
    <td  style="font-size: 7.5pt;">CCCGATCAACTCGAGTGCCACCTGACGGTCTAAGAAACCATTATTATCATGAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17tw</td>
 +
    <td>pSB6A1_Cm_rev_52</td>
 +
    <td>54</td>
 +
    <td>52.0</td>
 +
    <td  style="font-size: 7.5pt;">GGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCTGTCAGACCAAGTTTACTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17tx</td>
 +
    <td>mRFP-UBP-New_gaR</td>
 +
    <td>47</td>
 +
    <td>62.0</td>
 +
    <td  style="font-size: 7.5pt;">ATCGCTAGCACTGTACCTAGGACTGAGCTAGCACCGGTGGAGTGACG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ty</td>
 +
    <td>ccdB-UBP-Max_gaF</td>
 +
    <td>64</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">ACTAGTGAAAGAGGAGAAATACTAGATGGCTCAGTTTAAGGTTTACACCTATAAAAGAGAGAGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17tz</td>
 +
    <td>ccdB-UBP-New_gaF</td>
 +
    <td>64</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">ATAGTGAAAGAGCAGAAATACTAGATGGCTTCAGTTTAAGGTTTACACCTATAAAAGAGAGAGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ua</td>
 +
    <td>codA_lin_fwd</td>
 +
    <td>21</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">CATTAAAAGCGGTCGTTCCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ub</td>
 +
    <td>codA-lin_rev</td>
 +
    <td>20</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">CGGAATTTGCCACCAGAGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17uc</td>
 +
    <td>mRFP1_CBT-Asp_fw</td>
 +
    <td>35</td>
 +
    <td>70.0</td>
 +
    <td  style="font-size: 7.5pt;">TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ud</td>
 +
    <td>mRFP1_CBT-Asp_rv</td>
 +
    <td>26</td>
 +
    <td>70.0</td>
 +
    <td  style="font-size: 7.5pt;">CCTTTTTTGCCGGACTGCAGCGGCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ue</td>
 +
    <td>Cas9_Split_F</td>
 +
    <td>32</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">ATCTTGACTTTTCGCATCCCTTATTATGTTGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17uf</td>
 +
    <td>Cas9_Split_R</td>
 +
    <td>32</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">CCAACATAATAAGGGATGCGAAAAGTCAAGAT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ug</td>
 +
    <td>RBS_tight_F</td>
 +
    <td>43</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">TTTCACACAGGAATACTAGATGGCTTCCTCCGAAGACGTTATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17uh</td>
 +
    <td>RBS_tight_R</td>
 +
    <td>41</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CATCTAGTATTCCTGTGTGAAATTGTTATCCGCTCACAATT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ui</td>
 +
    <td>lacUV5_split_F</td>
 +
    <td>35</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">TATGCTTCCGGCTCGTATAATGTGTGAAATTGTGA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17uj</td>
 +
    <td>lacUV5_split_R</td>
 +
    <td>35</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">TCACAATTTCACACATTATACGAGCCGGAAGCATA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17uk</td>
 +
    <td>RBS_B0034_F</td>
 +
    <td>49</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">ACAATTAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATCA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ul</td>
 +
    <td>RBS_B0034_R</td>
 +
    <td>47</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CATCTAGTATTTCTCCTCTTTAATTGTTATCCGCTCACAATTTCACA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17um</td>
 +
    <td>RBS_B0035_F</td>
 +
    <td>52</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">AATTATTAAAGAGGAGAATACTAGATGGCTTCCTCCGAAGACGTTATCAAAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17un</td>
 +
    <td>RBS_B0035_R</td>
 +
    <td>49</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CATCTAGTATTCTCCTCTTTAATAATTGTTATCCGCTCACAATTTCACA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17uo</td>
 +
    <td>kan_alt_bb_1</td>
 +
    <td>23</td>
 +
    <td>64.0</td>
 +
    <td  style="font-size: 7.5pt;">TACTAGTAGCGGCCGCTGCAGTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17up</td>
 +
    <td>kan_alt_bb_2</td>
 +
    <td>18</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">CCCTTCGGGAAGCGTGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17uq</td>
 +
    <td>kan_alt_bb_3</td>
 +
    <td>21</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GGTCTGACAGCTCGAGTCCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ur</td>
 +
    <td>kan_alt_bb_4</td>
 +
    <td>24</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">CCGTTATTCATTCGTGATTGCGCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17us</td>
 +
    <td>kan_alt_bb_5</td>
 +
    <td>32</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">CACCCCTTGTATTACTGTTTATGTAAGCAGAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ut</td>
 +
    <td>pos_kanRr_fw              </td>
 +
    <td>53</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">AGCCTGCTCAACGAGCAGGCTTTTTTGCATGTATAAACGCAGAAAGGCCCACC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17uu</td>
 +
    <td>pos_B0015_rev            </td>
 +
    <td>63</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">CACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATATAAACGCAGAAAGGCCCACCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17uv</td>
 +
    <td>pos_kanRr_rev            </td>
 +
    <td>55</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">AAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGACGGCTAGCTCAGTCCTAGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17uw</td>
 +
    <td>pos_T7RNAP_fw            </td>
 +
    <td>69</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">CTTTGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAATTATGACAACTTGACGGCTACATCATTCAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ux</td>
 +
    <td>pos_tRNA_rev              </td>
 +
    <td>55</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">CACCTTCGGGTGGGCCTTTCTGCGTTTATACATGCAAAAAAGCCTGCTCGTTGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17uy</td>
 +
    <td>pSC_mRFP_fw</td>
 +
    <td>55</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">GTGCTAGTGTAGATCGCCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17uz</td>
 +
    <td>pSC_mRFP_rev</td>
 +
    <td>67</td>
 +
    <td>68.0</td>
 +
    <td  style="font-size: 7.5pt;">CTCCTCTTTCTCTAGTATCTCCCTTTAGTGAGTGTTAATTTACTAGTAGCGGCCGCTGCAGTCCGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17va</td>
 +
    <td>mRFP_pSC_fw</td>
 +
    <td>64</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">GGGAGATACTAGAGAAAGAGGAGAAATACTAGAGATGGCTTCCTCCGAAGACGTTATCAAAGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vb</td>
 +
    <td>mRFP_pSC_rev</td>
 +
    <td>49</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">CGGCCGCTTCTAGAGGCGATCTACACTAGCACTATCAGCGTTATTAAGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vc</td>
 +
    <td>T3Pol_2TAG_fw</td>
 +
    <td>55</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">ATGTAGTAGATCATCGAAAACATCGAAAAGAATGACTTCTCAGAAATCGAACTGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vd</td>
 +
    <td>T3Pol_2TAG_rev</td>
 +
    <td>67</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">CGATGTTTTCGATGATCTACTACATCTAGTACTTTCCTGTGTGACTCTAGTATTAGAAAAACTCATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ve</td>
 +
    <td>Bb-pRS_RFP_fw_61</td>
 +
    <td>24</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">GTTGATGTTGCTGTTGGAGGGATG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vf</td>
 +
    <td>Bb-pRS_RFP_rev_61</td>
 +
    <td>37</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">CAACCTTTGGATTTTCATCCTCTCTTGCTATAAGTTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vg</td>
 +
    <td>mRFP_RS_fw_60</td>
 +
    <td>58</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">GAACTTATAGCAAGAGAGGATGAAAATCCAAAGGTTGCAATACGCAAACCGCCTCTCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vh</td>
 +
    <td>mRFP_RS_rev_60</td>
 +
    <td>50</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">CTCCATCCCTCCAACAGCAACATCAACTATAAACGCAGAAAGGCCCACCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vi</td>
 +
    <td>158_159_162 _fw 55</td>
 +
    <td>73</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">GAGTATGGAACTTATAGCAAGAGAGGATGAAAATCCAAAGGTTGCTGAAGTTATCTATCCAATAATGATGGTT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vj</td>
 +
    <td>158-159-162 rev 55</td>
 +
    <td>77</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">CTCCATCCCTCCAACAGCAACATCAACGCCMNNATAATGCAGMNNMNNAACCATCATTATTGGATAGATAACTTCAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vk</td>
 +
    <td>S133_S155_fw_59</td>
 +
    <td>74</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">GCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCTAATGCAACCGGCGCAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vl</td>
 +
    <td>S155_S133_rev_59</td>
 +
    <td>61</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">GCGATCGCGTATTTCGTCTCGCTCAGGCGCAATAGCGAATGAATAACGGTTTGGTTGATGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vm</td>
 +
    <td>Prüf_S155_S133_rev_59</td>
 +
    <td>18</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">CTGCGCCGGTTGCATTAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vn</td>
 +
    <td>T7RNAP_spe_fw_59</td>
 +
    <td>63</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">CCGTTTTTTGGGCTAACAAAGAGGAGAAAGTAGTAATGTAGACGATTAACATCGCTAAGAACG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vo</td>
 +
    <td>T7RBS_Spe_rev_61</td>
 +
    <td>62</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">CGTTCTTAGCGATGTTAATCGTCTACATTACTACTTTCTCCTCTTTGTTAGCCCAAAAAACG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vp</td>
 +
    <td>T7RNAP_107_fw_59</td>
 +
    <td>48</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">GCCCGACAGCCTTCCAGTTCCTGCAATAGATCAAGCCGGAAGCCGTAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vq</td>
 +
    <td>T7RNAP_107_rev_59</td>
 +
    <td>52</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">GTGATGTACGCTACGGCTTCCGGCTTGATCTATTGCAGGAACTGGAAGGCTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vr</td>
 +
    <td>Prüf_T7RNAP_107_rev_59</td>
 +
    <td>19</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">CGGCTTCCGGCTTGATCTA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vs</td>
 +
    <td>gBlock_delta_own2_s</td>
 +
    <td>18</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">GTGAGCGATGCTCCGTTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vt</td>
 +
    <td>UBP_PCR_1C3_fw</td>
 +
    <td>21</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">CCTTAGCTTTCGCTAAGGATG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vu</td>
 +
    <td>UBP_PCR_1C3_rv</td>
 +
    <td>19</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">CGAATTGCAAGACGCTGTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vv</td>
 +
    <td>Prefix-AG_rev</td>
 +
    <td>20</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CTAGAAGCGGCCGCGAATTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vw</td>
 +
    <td>PtNTT2-AG_gaF</td>
 +
    <td>43</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">TTCTGGAATTCGCGGCCGCTTCTAGATGCGCCCGTATCCGACC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vx</td>
 +
    <td>codA-flank-part_gaF</td>
 +
    <td>46</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CTGGAATTCGCGGCCGCTTCTAGAGCATTAAAAGCGGTCGTTCCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vy</td>
 +
    <td>codA-flank-part_gaR</td>
 +
    <td>45</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CGGACTGCAGCGGCCGCTACTAGTACGGAATTTGCCACCAGAGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17vz</td>
 +
    <td>mRFP_sacB_gaR</td>
 +
    <td>55</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CTTGTTTTGCAAACTTTTTGATGTTTGCGGCTGCCGCAGCACCGGTGGAGTGACG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wa</td>
 +
    <td>sacB_ccdB-rep_gaF</td>
 +
    <td>57</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CGTCACTCCACCGGTGCTGCGGCAGCCGCAAACATCAAAAAGTTTGCAAAACAAGCA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wb</td>
 +
    <td>sacB-ccdB-rep_gaR</td>
 +
    <td>63</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">TAGTGACTCCTGCAGCGGCCGCTACTAGTATTATTTGTTAACTGTTAATTGTCCTTGTTCAAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wc</td>
 +
    <td>alt_VR_pSB1C3</td>
 +
    <td>21</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">CCTGCATAACGCGAAGTAATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wd</td>
 +
    <td>sacB-UBP-Max_gaF</td>
 +
    <td>60</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">GTACTAGTGAAAGAGGAGAAATACTAGATGGCTAACATCAAAAAGTTTGCAAAACAAGCA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17we</td>
 +
    <td>sacB-UBP-New_gaF</td>
 +
    <td>60</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">GTATAGTGAAAGAGCAGAAATACTAGATGGCTTAACATCAAAAAGTTTGCAAAACAAGCA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wf</td>
 +
    <td>retBB_sgRNA_gF</td>
 +
    <td>64</td>
 +
    <td>65.0</td>
 +
    <td  style="font-size: 7.5pt;">CGAAAAGCCTGCTCAACGAGCAGGCTTTTTTACTAGAGTTGACGGCTAGCTCAGTCCTAGGTAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wg</td>
 +
    <td>retBB_sgRNA_gR</td>
 +
    <td>58</td>
 +
    <td>66.0</td>
 +
    <td  style="font-size: 7.5pt;">GCTTAGTTGCATCTAGGTGACGCATCTCTCTCTCTAGAAGCGGCCGCGAATTCGAGTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wh</td>
 +
    <td>mRFP-retBB_o2_f</td>
 +
    <td>37</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">CTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wi</td>
 +
    <td>mRFP-retBB_o2_r</td>
 +
    <td>31</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">CCTATCATCGCTAGCACTGTACCTAGGACTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wj</td>
 +
    <td>Split_pSB3C5_for</td>
 +
    <td>26</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TGTATCACATATTCTGCTGACGCACC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wk</td>
 +
    <td>Split_pSB3C5_rev</td>
 +
    <td>26</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">GGTGCGTCAGCAGAATATGTGATACA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wl</td>
 +
    <td>Split_SpeI_for</td>
 +
    <td>40</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">TGCTAGCTGCTTGTGAAAGAGGAGAAATACTAGATGGCTT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wm</td>
 +
    <td>Split_SpeI_rev</td>
 +
    <td>40</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">TCCTCTTTCACAAGCAGCTAGCACTGTACCTAGGACTGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wn</td>
 +
    <td>lacUV5_Split_F</td>
 +
    <td>65</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">TATGCTTCCGGCTCGTATAATGGACAAAGAGGAGAAAGTAATGGATAAGAAATACTCAATAGGCT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wo</td>
 +
    <td>lacUV5_Split_R</td>
 +
    <td>60</td>
 +
    <td>53.0</td>
 +
    <td  style="font-size: 7.5pt;">CTCTTTGTCCATTATACGAGCCGGAAGCATAAAGTGTAAATATAAACGCAGAAAGGCCCA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wp</td>
 +
    <td>pSB1K3_Split_F</td>
 +
    <td>24</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">GTAAACTTGGTCTGACAGCTCGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wq</td>
 +
    <td>pSB1K3_Split_R</td>
 +
    <td>24</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">CTCGAGCTGTCAGACCAAGTTTAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wr</td>
 +
    <td>lacNEU_F</td>
 +
    <td>80</td>
 +
    <td>61.0</td>
 +
    <td  style="font-size: 7.5pt;">TCGGCTCGTATAATGTGTGAAATTGTGAGCGGATAACAATTTCACACAAGGAGATTACTAGATGGATAAGAAATACTCAA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ws</td>
 +
    <td>lacNEU_R</td>
 +
    <td>80</td>
 +
    <td>64.0</td>
 +
    <td  style="font-size: 7.5pt;">GTGAAATTGTTATCCGCTCACAATTTCACACATTATACGAGCCGATGATTAATTGTCAACAGCCTGGGGTGCCTAAATTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wt</td>
 +
    <td>lacNEU_BB_F</td>
 +
    <td>46</td>
 +
    <td>67.0</td>
 +
    <td  style="font-size: 7.5pt;">ACAATTTCACACAAGGAGATTACTAGTAGCGGCCGCTGCAGTCCGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wu</td>
 +
    <td>lacNEU_BB_R</td>
 +
    <td>53</td>
 +
    <td>69.0</td>
 +
    <td  style="font-size: 7.5pt;">AATTCTCACGCTTTCCAGTCCTCTAGAAGCGGCCGCGAATTCCAGAAATCATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wv</td>
 +
    <td>ColPCR_Mis_F</td>
 +
    <td>22</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">CCAGGCTGTTGACAATTAATCA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ww</td>
 +
    <td>ColPCR_R</td>
 +
    <td>18</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">CCTTCTACGAGCTGTCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wx</td>
 +
    <td>ColPCR2_F</td>
 +
    <td>20</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">CCGGGAGGCATTTACTAGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wy</td>
 +
    <td>c3_g1_i4_fw</td>
 +
    <td>27</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CTTAAAGGTGTTGAACATGGTGAGATG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17wz</td>
 +
    <td>c2_g1_i4_rv</td>
 +
    <td>25</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">ACCATGTTCAACACCTTTAAGCTTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xa</td>
 +
    <td>c3g1i4_gBl_gF</td>
 +
    <td>68</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCATTAGATCCTAAGGTTAAAGATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xb</td>
 +
    <td>c3g1i4_gBl_gR</td>
 +
    <td>58</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCTCCCACTCAATGGTCGAAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xc</td>
 +
    <td>pSB3C5_split_fwd</td>
 +
    <td>20</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">GTTTCGCCACCACTGATTTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xd</td>
 +
    <td>pSB3C5_split_rev</td>
 +
    <td>20</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">ACGCTCAAATCAGTGGTGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xe</td>
 +
    <td>pSB3C5-SpeI-remo-gaF</td>
 +
    <td>50</td>
 +
    <td>63.0</td>
 +
    <td  style="font-size: 7.5pt;">TACAGTGCTAGCTCATAGTGAAAGAGGAGAAATACTAGATGGCTTCCTCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xf</td>
 +
    <td>pSB3C5-SpeI-remo_gaR</td>
 +
    <td>45</td>
 +
    <td>60.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGTATTTCTCCTCTTTCACTATGAGCTAGCACTGTACCTAGGAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xg</td>
 +
    <td>sacB-Test-gaF</td>
 +
    <td>43</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">GCGGCAGCCGCAGCAGCCGCAACCCGCGATACCAAAGATACCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xh</td>
 +
    <td>mRFP-Test-gaR</td>
 +
    <td>41</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">TGCGGCTGCTGCGGCTGCCGCAGCACCGGTGGAGTGACGAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xi</td>
 +
    <td>sacB-test-gaF</td>
 +
    <td>47</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CGTTCGGACTTCACACGGCCAACATCAAAAAGTTTGCAAAACAAGCA</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xj</td>
 +
    <td>sacB-test-gaR</td>
 +
    <td>46</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">GGCCGTGTGAAGTCCGAACGACCACGCTCACGCTGCTATAAATTTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xk</td>
 +
    <td>pTXB1_GMPSopt_f</td>
 +
    <td>59</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCGCTGGATCCAAAAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xl</td>
 +
    <td>pTXB1_GMPSopt_r</td>
 +
    <td>61</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGTTCCCATTCGATTGTCGACGGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xm</td>
 +
    <td>pTXB1_CDAopt_f</td>
 +
    <td>69</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAACAATATCGCTTTGTAATTGAAGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xn</td>
 +
    <td>pTXB1_CDAopt_r</td>
 +
    <td>68</td>
 +
    <td>59.0</td>
 +
    <td  style="font-size: 7.5pt;">CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGGCAGCTTTTGTTAGATGAACAATGAAACAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xo</td>
 +
    <td>phyto_fw</td>
 +
    <td>38</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">AACGTCTCACTCGATCGATGCAAGCCAAACCATTTCAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xp</td>
 +
    <td>phyto_rv</td>
 +
    <td>53</td>
 +
    <td>54.0</td>
 +
    <td  style="font-size: 7.5pt;">AACGTCTCACTCAATCGTCAATTGAAATTTAAATCTAATGTATCAATTGAATC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xq</td>
 +
    <td>GFP-NPtNTT2_gaF</td>
 +
    <td>58</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">GCTCGTATAATGGACAAAGAGGAGAAAGTAATGCGTAAAGGAGAAGAACTTTTCACTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xr</td>
 +
    <td>PtNTT2-NGFP_gaR</td>
 +
    <td>49</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">AGTTCTTCTCCTTTACGCATTACTTTCTCCTCTTTGTCCATTATACGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xs</td>
 +
    <td>GFP-NPtNTT2_gaR</td>
 +
    <td>64</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">GTCGGATACGGGCGTGCGGCTGCCGCTGCGGCTGCCGCTTTGTATAGTTCATCCATGCCATGTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xt</td>
 +
    <td>PtNTT2-NGFP_gaF</td>
 +
    <td>58</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">GATGAACTATACAAAGCGGCAGCCGCAGCGGCAGCCGCACGCCCGTATCCGACCATTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xu</td>
 +
    <td>PtNTT2_seq2_fwd</td>
 +
    <td>20</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">GGCCTGGTGTTTGTGTTTGG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xv</td>
 +
    <td>PtNTT2(31-575)-T7-gaF</td>
 +
    <td>43</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">TAATACGACTCACTATAGGGAAAGAGGAGAAAGTAATGCTGCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xw</td>
 +
    <td>PtNTT2-T7-gaF</td>
 +
    <td>43</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">TAATACGACTCACTATAGGGAAAGAGGAGAAAGTAATGCGCCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>7xx</td>
 +
    <td>PtNTT2-T7-gaR</td>
 +
    <td>47</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">TCTCCTCTTTCCCTATAGTGAGTCGTATTACTCTAGAAGCGGCCGCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xy</td>
 +
    <td>MHA-mRFP-seqR</td>
 +
    <td>20</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">ACCTTCGTACGGACGACCTT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17xz</td>
 +
    <td>CWH-mRFP_seqF</td>
 +
    <td>20</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CGGGTGCTTACAAAACCGAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ya</td>
 +
    <td>CWH-sacB-seqR</td>
 +
    <td>21</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CGTGTAATATGGGAAATGCCG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17yb</td>
 +
    <td>mRFPretBB_25_gF</td>
 +
    <td>25</td>
 +
    <td>56.0</td>
 +
    <td  style="font-size: 7.5pt;">GAAAGAGGAGAAATACTAGATGGCT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17yc</td>
 +
    <td>mRFPretBB_25_gR</td>
 +
    <td>21</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">AGCACTGTACCTAGGACTGAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17yd</td>
 +
    <td>PtNTT2_histag_gaF</td>
 +
    <td>48</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CACCATCACCACCATCATCACCATTAATAATACTAGTAGCGGCCGCTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ye</td>
 +
    <td>PtNTT2_histag_gaR</td>
 +
    <td>55</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">ATGGTGATGATGGTGGTGATGGTGATGATGTTTTTCTTTGTTGGTTTTCGCTTCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17yf</td>
 +
    <td>Seq_Library_fw</td>
 +
    <td>90</td>
 +
    <td>57.0</td>
 +
    <td  style="font-size: 7.5pt;">AATGGGGTTAAAGGCAAAATATGTTTATGGAAGTGAATTCCAGCTTGATAAGGATTATACACTGAATGTCTATAGATTGGCTTTAAAAAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17yg</td>
 +
    <td>Seq_Library_rev</td>
 +
    <td>81</td>
 +
    <td>58.0</td>
 +
    <td  style="font-size: 7.5pt;">CTGCCCAGCTGGAGTTGTTGAAGGAAATCCAATAATGGAGATAGCTAAATACTTCCTTGAATATCCTTTAACCATAAAAAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17yh</td>
 +
    <td>ColPCR_PtNTT2_F</td>
 +
    <td>21</td>
 +
    <td>55.0</td>
 +
    <td  style="font-size: 7.5pt;">AAAATGGAAGCGAAAACCAAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17yi</td>
 +
    <td>split_kan_fw</td>
 +
    <td>43</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">GTGCCCTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17yj</td>
 +
    <td>split_kan_rv</td>
 +
    <td>53</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">TCGTCTTGCAGTTCATTCAGGGCACCGGACAGGTCGGTCTTGACAAAAAGAAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17yk</td>
 +
    <td>split_kan_ggü_fw</td>
 +
    <td>29</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">GGCGGTAATACGGTTATCCACAGAATCAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17yl</td>
 +
    <td>split_kan_ggü_rv</td>
 +
    <td>29</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CTGATTCTGTGGATAACCGTATTACCGCC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ym</td>
 +
    <td>Seq_57_fw_B8</td>
 +
    <td>89</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNCAGCTTGATAAGGATTATACACTGAATGTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17yn</td>
 +
    <td>Seq_58_fw_B11</td>
 +
    <td>92</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNGCTTGATAAGGATTATACACTGAATGTCTATAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17yo</td>
 +
    <td>Seq_57_fw_B21</td>
 +
    <td>89</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNGGATTATACACTGAATGTCTATAGATTGGC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17yp</td>
 +
    <td>Seq_57_fwd_B3</td>
 +
    <td>90</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNCACTGAATGTCTATAGATTGGCTTTAAAAAC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17yq</td>
 +
    <td>Seq_58_B8_rev</td>
 +
    <td>81</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCACAACTCCAGCTGGGCAG</td>
 +
    </tr>
 +
<tr>
 +
    <td>17yr</td>
 +
    <td>Seq_57_alt_rev</td>
 +
    <td>87</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCNGATTTCCTTCAACAACTCCAGCT</td>
 +
    </tr>
 +
<tr>
 +
    <td>17ys</td>
 +
    <td>Seq_57_B11_rev</td>
 +
    <td>91</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CAAGCAGAAGACGGCATACGAGATGTAGCCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCCATTATTGGATTTCCTTCAACAACTC</td>
 +
    </tr>
 +
<tr>
 +
    <td>17yt</td>
 +
    <td>Seq_56_B21_rev</td>
 +
    <td>93</td>
 +
    <td>nan</td>
 +
    <td  style="font-size: 7.5pt;">CAAGCAGAAGACGGCATACGAGATCGAAACGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCGGATATTCAAGGAAGTATTTAGCTATCTCC</td>
 +
    </tr>
 +
</tbody>
 +
</table>
 +
</div>
 +
</div>
 +
 +
 +
 +
  
 
</div>
 
</div>

Latest revision as of 18:09, 27 October 2017

Oligonucleotides
ID Name Length Tm °C Sequence 5'-> 3'
17aa tetM_gaF 48 61.0 ACAATGCTAGCGACAAAGAGGAGAAAGTAATGCGCACCCTGAACATCG
17ab tetM_gaR 49 60.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTACTACTCACCCGAATGCCCG
17ac tetM_gaF 60 56.0 ACAATGCTAGCGACAAAGAGGAGAAAGTAATGAAAATCATAAATATCGGTGTATTAGCTC
17ad tetM_gaR 60 55.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATGTGATTTTGTTGAACATATAACGAAC
17ae tetM_gaF 64 56.0 TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAAAATTATTAATATTGGAGTTTTAGCTCATG
17af tetM_gaR 74 55.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTAAGTTATTTTATTGAACATATATCTTACTTTATCTATCC
17ag pSB1C3-mRFP_gaF 49 68.0 CTAGTGTAGATCGCGGTCTCATGCGTACTAGTAGCGGCCGCTGCAGTCC
17ah pSB1C3-mRFP_gaR 73 68.0 CTCCTCTTTGTCGCTAGCATTGTACCTAGGACTGAGCTAGCCATAAACTCTAGAAGCGGCCGCGAATTCCAGA
17ai aac2_gaF 49 62.0 TACAATGCTAGCGACAAAGAGGAGAAAGTAATGACCATCGACCTCCGCC
17aj aac2_gaR 48 62.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGGTGACGTCGCCGTC
17ak mRFP_gaF 68 58.0 CTAGGTACAATGCTAGCGACAAAGAGGAGAAAGTAATGGGCGAGACCGCTTCCTCCGAAGACGTTATC
17al mRFP_gaR 65 58.0 TTTTGCCGGACTGCAGCGGCCGCTACTAGTACGCATGAGACCGCGATCTACACTAGCACTATCAG
17am aadA_gaF 49 60.0 TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAGGGAAGCGGTGATCG
17an aadA_gaR 58 59.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTGCCGACTACCTTGGTGATCTCG
17ao ermc_gaF 62 57.0 ACAATGCTAGCGACAAAGAGGAGAAAGTAATGAACGAGAAAAATATAAAACACAGTCAAAAC
17ap ermc_gaR 78 58.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATTACTTATTAAATAATTTATAGCTATTGAAAAGAGATAAGAATTGTTC
17aq msrA_gaF 67 58.0 ACAATGCTAGCGACAAAGAGGAGAAAGTAATGGAACAATATACAATTAAATTTAACCAAATCAATCA
17ar msrA_gaR 67 58.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATTAAGTTATATCATGAATAGATTGTCCTGTTAATTCC
17as mef_gaF 62 58.0 ACAATGCTAGCGACAAAGAGGAGAAAGTAATGGAAAAATACAACAATTGGAAACTTAAGTTT
17at mef_gaR 71 58.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTTAAATCTAATTTTCTAATCTCATTTATCATTGGGC
17au ermX_gaF 48 58.0 ACAATGCTAGCGACAAAGAGGAGAAAGTAATGTCTGCATACGGACACG
17av ermX_gaR 51 58.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATCACCTGGAAGAGATCGATCC
17aw carB4_gaF 54 60.0 TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAAGTTGCTGTTAGTGTTCTCG
17ax carB4_gar 53 59.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAACGGCTTTGCGAACTATAAA
17ay tetX_gaF 47 61.0 TGCTAGCGACAAAGAGGAGAAAGTAATGAGACCTCGTATCGCTGTGG
17az tetX_gaR 43 62.0 CGGACTGCAGCGGCCGCTACTAGTATCAAGCAGCAGGGCAGCG
17ba addA1_gaF 55 59.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTGCCGACTACCTTGGTGATC
17bb addA1_gaR 49 60.0 TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAGGGAAGCGGTGATCG
17bc addA1_gaF 49 59.0 TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAGGGAAGCGGTGATCG
17bd addA1_gaR 53 58.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTGCCGACTACCTTGGTGA
17be aad9_gaF 52 57.0 GCTAGCGACAAAGAGGAGAAAGTAATGAGGAGGATATATTTGAATACATACG
17bf aad9_gaR 73 54.0 CGGACTGCAGCGGCCGCTACTAGTATTATAATTTTTTTAATCTGTTATTTAAATAGTTTATAGTTAAATTTAC
17bg aac3_gaF 53 61.8 TACAATGCTAGCGACAAAGAGGAGAAAGTAGTGCAATACGAATGGCGAAAAGC
17bh aac3_gaR 50 62.6 TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGCCAATCGACTGGCGAG
17bi tetA_gaF 55 59.0 TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAAATCTAACAATGCGCTCATCG
17bj tetA_gaR 48 60.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATTAGGTCGAGGTGGCCCG
17bk pbp5_gaF 57 59.0 TACAATGCTAGCGACAAAGAGGAGAAAGTAATGGAACGAAGCAATCGTAATAAAAAG
17bl pbp5_gaR 65 59.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTAATGGTTGCTTCTAAGTAATCAATTAATG
17bm hyg_gaF 61 66.7 TACAATGCTAGCGACAAAGAGGAGAAAGTAGTGACACAAGAATCCCTGTTACTTCTCGACC
17bn hyg_gaR 46 67.9 TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGGCGCCGGGGGCG
17bo arpA_gaR 51 61.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGCCTTTCGGTGTTTCCAC
17bp arpA_gaF 50 61.0 TACAATGCTAGCGACAAAGAGGAGAAAGTAATGCAATTCAAGCCAGCCGT
17bq ecoRI(1)35 35 63.0 GAAGGTCGCCTGGAGTTCTCCGAAGTCGCGGTGGA
17br ecoRI(1)re 35 63.0 GCGACTTCGGAGAACTCCAGGCGACCTTCCTGCTT
17bs ecoRI(2)fw 36 64.0 CATTCTGATCGTGGAGTTCGCCAAGGAGCTGCACGA
17bt ecoRI(2)re 40 64.0 AGCTCCTTGGCGAACTCCACGATCAGAATGGCGTTTTTCG
17bu PlacUV5_ga1F 55 56.0 TTTACACTTTATGCTTCCGGCTCGTATAATGGACAAAGAGGAGAAAGTAATGGGC
17bv PlacUV5_ga1R 19 58.0 CAGCGGCCGCTACTAGTAC
17bw PlacUV5_ga2F 19 58.0 GTACTAGTAGCGGCCGCTG
17bx PlacUV5_ga2R 48 58.0 CATTATACGAGCCGGAAGCATAAAGTGTAAACTCTAGAAGCGGCCGCG
17by PtNTT2_gaF 47 60.0 GCTCGTATAATGGACAAAGAGGAGAAAGTAATGCGCCCGTATCCGAC
17bz PtNTT2_gaR 58 57.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTTTCTTTGTTGGTTTTCGCTTCC
17ca LFS_ovexPCR_F 51 58.0 TCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCC
17cb LFS_ovexPCR_R 50 57.0 ACTGAGCCTTTCGTTTTATTTGATGCCTGGAATGCCTCCCGGATAAAACA
17cc Term_ovexPCR_F 53 57.0 TTTTCAGATGTGTTTTATCCGGGAGGCATTCCAGGCATCAAATAAAACGAAAG
17cd Term_ovexPCR_R 50 57.0 GAGCGCTCACAATTCTCACGCTTTCCAGTCTATAAACGCAGAAAGGCCCA
17ce lacO_ovexPCR_F 41 58.0 TGGGCCTTTCTGCGTTTATAGACTGGAAAGCGTGAGAATTG
17cf lacO_ovexPCR_R 57 57.0 ATCTAAGCCTATTGAGTATTTCTTATCCATCTAGTATTCCTGTGTGAAATTGTTATC
17cg cas9_ovexPCR_F 58 57.0 GCGGATAACAATTTCACACAGGAATACTAGATGGATAAGAAATACTCAATAGGCTTAG
17ch cas9_ovexPCR_R 80 57.0 ATGGTAATGTTGCTCATATCAGTATCTCATTTTACGGCGGTCGCCTTGACCGCCGACACCCTCAGTCACCTCCTAGCTGA
17ci RFS_ovexPCR_F 80 57.0 GAGTCAGCTAGGAGGTGACTGAGGGTGTCGGCGGTCAAGGCGACCGCCGTAAAATGAGATACTGATATGAGCAACATTAC
17cj RFS_ovexPCR_R 59 58.0 TTAGAACAGGAATGGATTTTCTTCTACACACAATAAATGTAATGCGTTATATCTTCCTG
17ck Sequ_ftsz_R 23 53.0 GTAAGTTCCATTGGTTCAAACAT
17cl Sequ_ftsz_F 21 55.0 CTGCGTAAGCAAGCTGATTAA
17cm Sequ_tet_R 21 53.0 GAGCGCATTGTTAGATTTCAT
17cn Sequ_tet_F 20 65.0 GCCGGGCCACCTCGACCTAA
17co Sequ_kan_R 21 50.0 CAATCCATCTTGTTCAATCAT
17cp Sequ_kan_F 21 53.0 CTTCTTGACGAGTTCTTCTGA
17cq codA_flanke1_delF 46 59.0 TGAGTTAGCTCACTCATTAGGCACCCATTAAAAGCGGTCGTTCCCG
17cr codA_flanke1_delR 27 60.0 TTATTCGACACTGTTAGCCTCCACATG
17cs codA_flanke2_delF 44 60.0 TGTGGAGGCTAACAGTGTCGAATAAACGACTGGGTTACAGCGAG
17ct codA_flanke2_delR 45 58.0 GACTGGCTTTCTACGTGTTCCGCTTCGGAATTTGCCACCAGAGAG
17cu sgRNA_LKA01_for 24 0.0 ACGCTATTGTTCATAATAGAAGAG
17cv sgRNA_LKA01_rev 24 0.0 AAACCTCTTCTATTATGAACAATA
17cw PtNTT2_assembly_gaF 20 60.0 GCGGCGGATAACGAACAGAG
17cx PtNTT2_assembly_gaR 23 58.0 TAATGCTCTGTTCGTTATCCGCC
17cy Sequ_MCS_F 20 54.0 GCGAATTGGGTACACTTACC
17cz Sequ_MCS_R 21 54.0 CACTAAAGGGAACAAAAGCTG
17da Sequ_pBK1_F 20 64.0 CACCTGACGCGCCCTGTAGC
17db Sequ_pBK2_F 24 54.0 GCATTCATTTTATGTTTCAGGTTC
17dc Sequ_pBK3_F 21 54.0 CAGCTTTTGTTCCCTTTAGTG
17dd Sequ_pBK4_F 21 54.0 CAGAAGAACTCGTCAAGAAGG
17de Sequ_54_F 22 59.0 GAACGCTCTCCTGAGTAGGACA
17df Sequ_55_F 21 66.0 GGCGCAGCGGTCGGACTGAAC
17dg Sequ_56_F 21 64.0 CTGCCCTGCTGAACTACCGCC
17dh Sequ_58_R 21 60.0 GTAATCGCTGAATTCCCGGCG
17di Prefix_fwd 19 59.0 TTTCTGGAATTCGCGGCCG
17dj Suffix_rev 17 60.0 TTTGCCGGACTGCAGCG
17dk KanMod-Fwd-1 57 61.0 CATAAACAGTAATACAAGGGGTGTTATGTAGAGCCATATTCAACGGGAAACGTCTTG
17dl KanMod-Fwd-2 60 61.0 CATAAACAGTAATACAAGGGGTGTTATGTAGTAGAGCCATATTCAACGGGAAACGTCTTG
17dm KanMod-Fwd-3 63 60.0 CATAAACAGTAATACAAGGGGTGTTATGTAGTAGTAGAGCCATATTCAACGGGAAACGTCTTG
17dn KanMod-Rev 32 61.0 AACACCCCTTGTATTACTGTTTATGTAAGCAG
17do KanMod-Split-Fwd 33 58.0 GTAGTGATCTTATTTCATTATGGTGAAAGTTGG
17dp KanMod-Split-Rev 33 58.0 CCAACTTTCACCATAATGAAATAAGATCACTAC
17dq KanMod-19ML 24 55.0 GCTTAAACTCCAACTTGGACGCTG
17dr LacO_GA_For 52 61.0 ATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAG
17ds LacO_GA_Rev 49 61.0 GGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAG
17dt VR 20 56.0 GCTCACTCAAAGGCGGTAAT
17du VF2 20 57.0 TGCCACCTGACGTCTAAGAA
17dv pK18-wo-ori_fwd 14 59.0 ACGCCAGCAACGCG
17dw pK18-wo-ori_rev 29 60.0 GAAGATCCTTTGATCTTTTCTACGGGGTC
17dx pSC101-replicon-gaF 45 56.0 CCGTAGAAAAGATCAAAGGATCTTCGAGTTATACACAGGGCTGGG
17dy pSC101-replicon_gaR 49 57.0 CGTAAAAAGGCCGCGTTGCTGGCGTTCAGATCCTTCCGTATTTAGCCAG
17dz RubiscoTAG2_fw 50 68.0 ATGCAGGATTACAAGCAAAGCCTCAAATATGAGACTTTCTCTTATCTTCC
17ea RubiscoTAG2_rv 60 68.0 TGAGGCTTTGCTTGTAATCCTGCATCTAAGCCATGATATCTTTCTCCTCTTTCTCTAGAA
17eb RubiscoTAG111_fw 52 68.0 AAAGAGGAGAAATACTAGATGGCAGTTAAGAAATATAGCGCGGGTGTCAAAG
17ec RubiscoTAG111_rev 57 68.0 CTGCCATCTAGTATTTCTCCTCTTTGATATCTTACTAGTTGCCGCGGTAGACCACGA
17ed RubisoTAG474_fw 45 68.0 TACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTC
17ee RubisoTAG474_rev 60 68.0 CGGACTGCAGCGGCCGCTACTAGTAGATATCTTACTAACGGTTTTGTGTGTCCAACTTAT
17ef RubisomRFP_fw 42 64.0 CTTCGTGGTCTACCGCGGCAACTACTAGAGAATGGCTTCCTC
17eg RubiscomRFP_rev 56 63.0 GCGCTATATTTCTTAACTGCCATCTAGTATTTCTCCTCTTTGCGATCTACACTAGC
17eh mRFPRubisco_fw 36 54.0 GATAGTGCTAGTGTAGATCGCAAAGAGGAGAAATAC
17ei mRFPRubisco_rev 33 55.0 GTCTTCGGAGGAAGCCATTCTCTAGTAGTTGCC
17ej Sequ_pBAD_fw 18 62.0 GTTGCGTCGCGGTGCATG
17ek Sequ-pBAD_rev 20 58.0 CAATCTCACCATTCGCGGTC
17el VR 20 56.0 ATTACCGCCTTTGAGTGAGC
17em LFS_GA_F 64 65.0 AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCATTGCCAGAGTGAAAATGCCTCAGAGCT
17en LFS_GA_R 74 65.0 CCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAAATGCCTCCCGGATAAAACACATCTGAAAATTCATATGT
17eo BB_LFS_F 56 69.0 TTTTCAGATGTGTTTTATCCGGGAGGCATTTACTAGTAGCGGCCGCTGCAGTCCGG
17ep BB_LFS_R 64 68.0 TAGCTCTGAGGCATTTTCACTCTGGCAATGCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC
17eq Term_GA_F 57 68.0 ATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCCAGG
17er Term_GA_R 67 69.0 GGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGG
17es BB_Term_F 56 69.0 CACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTCCGG
17et BB_Term_R 64 68.0 ACTGAGCCTTTCGTTTTATTTGATGCCTGGCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC
17eu lacO_GA_F2 62 68.0 ATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGACTGGAAAG
17ev lacO_GA_R2 67 69.0 GGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTCCTGTGTGAAATTG
17ew BB_lacO_F 56 69.0 TTGTGAGCGGATAACAATTTCACACAGGAATACTAGTAGCGGCCGCTGCAGTCCGG
17ex BB_lacO_R 64 68.0 GAGCGCTCACAATTCTCACGCTTTCCAGTCCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC
17ey Cas9_GA_F 69 58.0 AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGATGGATAAGAAATACTCAATAGGCTTAGATATCG
17ez Cas9_GA_R 80 58.0 GCCGGACTGCAGCGGCCGCTACTAGTATTTTACGGCGGTCGCCTTGACCGCCGACACCCTCAGTCACCTCCTAGCTGACT
17fa BB_Cas9_F 80 69.0 GAGTCAGCTAGGAGGTGACTGAGGGTGTCGGCGGTCAAGGCGACCGCCGTAAAATACTAGTAGCGGCCGCTGCAGTCCGG
17fb BB_Cas9_R 64 68.0 ATCTAAGCCTATTGAGTATTTCTTATCCATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC
17fc RFS_GA_F 74 62.0 AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTGAGATACTGATATGAGCAACATTACCATTTATCACAAC
17fd RFS_GA_R 80 62.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTACAATAAATGTAATGCGTTATATCTTCCTGATATAATTACCTTTGATTTCC
17fe BB_RFS_F 56 69.0 TCAGGAAGATATAACGCATTACATTTATTGTACTAGTAGCGGCCGCTGCAGTCCGG
17ff BB_RFS_R 64 68.0 AATGGTAATGTTGCTCATATCAGTATCTCACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC
17fg PtNTT2_trunc_gaF 45 60.0 TCGTATAATGGACAAAGAGGAGAAAGTAATGGCGGCAGCACCGTG
17fh aaRS_pC3_fw 37 60.0 CGGCCGCTTCTAGAGGCACCTGAAGTCAGCCCCATAC
17fi aaRS_RuTAG_rev 40 60.0 TATCTTTCTCCTCTTTGTGCAAGATTCCGAATACCGCAAG
17fj RuTAG_aaRS_fw 38 54.0 TTCGGAATCTTGCACAAAGAGGAGAAAGATATCATGGC
17fk RuTAG474_pC3_rv 40 54.0 CGGCCGCTACTAGTAGATATCTTACTAACGGTTTTGTGTG
17fl pC3_RuTAG474_fw 45 70.0 CGTTAGTAAGATATCTACTAGTAGCGGCCGCTGCAGTCCGGCAAA
17fm pC3_aaRS_rev 55 70.0 GCTGACTTCAGGTGCCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGCG
17fn pC3_RuTAG_fw 45 70.0 CACACAAAACCGTTAATACTAGTAGCGGCCGCTGCAGTCCGGCAA
17fo Sequ_pBad3 20 54.0 GCACCACCCGGATGCCTATC
17fp Sequ_pBad4 21 54.0 GTCCGCACCGCTGCCGGTAGC
17fq RuTAG_pC3_rv 37 54.0 TTGGACACACAAAACCGTTAATACTAGTAGCGGCCGC
17fr pSB1C3_splitF 20 59.0 GTCTGACAGCTCGAGGCTTG
17fs pSB1C3_splitR 20 59.0 CAAGCCTCGAGCTGTCAGAC
17ft PlacUV5_ga2R_v2 56 54.0 TCTTTGTCCATTATACGAGCCGGAAGCATAAAGTGTAAACTCTAGAAGCGGCCGCG
17fu PlacUV5_ga2F_v2 18 57.0 TACTAGTAGCGGCCGCTG
17fv pRedET_SeqF_1 20 57.0 AGGCGTAGCAGAAACTAACG
17fw pRedET_SeqF_2 20 56.0 CACACAAAGGTCTAGCGGAA
17fx pRedET_SeqR_1 18 59.0 GACGCAGTGACGGCAATG
17fy pRedET_SeqR_2 20 58.0 ATTTCAGCGACCTGTTTGGG
17fz pK18msb_ga1 20 58.0 AAGCGGAACACGTAGAAAGC
17ga pK18msb_ga2 22 57.0 GGTGCCTAATGAGTGAGCTAAC
17gb K1172904fw_gb1 51 58.0 GATTTCTGGAATTCGCGGCCGCTTCTAGAGATGGCAGCACAGGTTATCAAC
17gc K1172904re_gb2 60 57.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATCTGATTTTTGTAAAGGTCTGATAATG
17gd psB1A3fw_gb3 51 64.0 CATTATCAGACCTTTACAAAAATCAGATAATACTAGTAGCGGCCGCTGCAG
17ge psB1A3re_gb4 51 63.0 GTCAAACGTGTTGATAACCTGTGCTGCCATCTCTAGAAGCGGCCGCGAATT
17gf Glu107_fw 63 67.0 CTGAAAGCGAAATACGTCTATGGCAGCNNKGAACAGCTGGACAAAGACTACACCCTGAACGTG
17gg Glu107_rev 64 67.0 GCTGCCATAGACGTATTTCGCTTTCAGGCCCATCGCTTCGAACACTTTCTTGTTGTAGTCGCCG
17gh Asp158_Ile159_Leu162_fw 73 67.0 GAAGTGATCTATCCGATCATGCAGGTGAACNNKNNKCACTATNNKGGCGTAGATGTAGCAGTTGGTGGCATGG
17gi Asp158_Ile159_Leu162_rev 59 67.0 GTTCACCTGCATGATCGGATAGATCACTTCCGCCACTTTCGGGTTTTCATCTTCGCGGG
17gj pSB1C3_lin_fwd 19 59.0 TACTAGTAGCGGCCGCTGC
17gk GFPBB_fwd 51 58.0 CTGGAATTCGCGGCCGCTTCTAGAGATGCGTAAAGGAGAAGAACTTTTCAC
17gl pSB1C3_lin_rev 17 61.0 CTCTAGAAGCGGCCGCG
17gm GFPLink1_rev 64 58.0 CCCACCACTGCCTCCACTACCTCCAGAGCCCCCTTTGTATAGTTCATCCATGCCATGTGTAATC
17gn GFPLink2_rev 64 58.0 CCCACCACTGCCTCCCTAACCTCCAGAGCCCCCTTTGTATAGTTCATCCATGCCATGTGTAATC
17go Link1Step_fwd 54 61.0 GGGGGCTCTGGAGGTAGTGGAGGCAGTGGTGGGGCTGAAGCTGGTATCACCGGC
17gp Link2Step_fwd 54 61.0 GGGGGCTCTGGAGGTTAGGGAGGCAGTGGTGGGGCTGAAGCTGGTATCACCGGC
17gq StrepBB_rev 45 61.0 CTGCAGCGGCCGCTACTAGTATTAGGAAGCAGCGGACGGTTTAAC
17gr sup35_Histag_fw 66 70.0 CATCATCACCATCACCACCATCATCACCATTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAA
17gs sup35_Histag_rev 67 69.0 GATGGTGGTGATGGTGATGATGAACTTCGTCGTCAACTTCTTCTTCCTGTTCTTTGATCAGAGCGTC
17gt pSB1C3_sup35_fw 51 70.0 GAAGTTGACGACGAAGTTTAATACTAGTAGCGGCCGCTGCAGTCCGGCAAA
17gu pSB1C3_sup35_rv 56 70.0 CCTGGTTAGAGTCAGACATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTA
17gv T7RNAP_fw 59 64.0 GATTTCTGGAATTCGCGGCCGCTTCTAGAGATGAACACGATTAACATCGCTAAGAACGA
17gw T7RNAP_re 50 64.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATTACGCGAACGCGAAGTCCG
17gx psB1C3_T7P_fw 50 63.0 ATCTTAGAGTCGGACTTCGCGTTCGCGTAATACTAGTAGCGGCCGCTGCA
17gy psB1C3_T7P_re 50 63.0 GTCGTTCTTAGCGATGTTAATCGTGTTCATCTCTAGAAGCGGCCGCGAAT
17gz PtNTT2_seq_1 20 59.0 GGCAACCAGGAAGTGACCAA
17ha PtNTT2_seq_2 20 60.0 CACATCAATCGCCGCTTTGC
17hb PromRBS-Lin-fwd 25 65.0 TACTAGTAGCGGCCGCTGCAGTCCG
17hc KanR-Prä-fwd 54 61.0 ATGCTAGCTACTAGAGTCACACAGGAAAGATGAGCCATATTCAACGGGAAACGT
17hd PromRBS-Lin-rev 23 57.0 CTTTCCTGTGTGACTCTAGTAGC
17he KanMod-19ML-fwd 43 65.0 TCAGCATCCAAGTTGGAATTTAATCGCGGCCTGGAGCAAGACG
17hf KanMod-19ML-rev 43 53.0 GCGATTAAATTCCAACTTGGATGCTGATTTATATGGGTATAAA
17hg Suff-KanR-rev 52 59.0 TGCAGCGGCCGCTACTAGTAATTAGAAAAACTCATCGAGCATCAAATGAAAC
17hh neuKan-mod-TAG1 49 59.0 ACTAGAGTCACACAGGAAAGATGTAGAGCCATATTCAACGGGAAACGTC
17hi neuKan-mod-TAG2 52 59.0 ACTAGAGTCACACAGGAAAGATGTAGTAGAGCCATATTCAACGGGAAACGTC
17hj neuKan-mod-TAG3 55 59.0 ACTAGAGTCACACAGGAAAGATGTAGTAGTAGAGCCATATTCAACGGGAAACGTC
17hk ONBY-out-rev 26 54.0 ATGAATCTCCAAGTATATAACGACTG
17hl ONBY-out-fwd 27 63.0 TAAGATCTGGATCCTCTACGCCGGACG
17hm NPA/ONBY-switch-fwd 46 59.0 TTATATACTTGGAGATTCATATGGACGAGTTTGAGATGATTAAGCG
17hn ONBY/NPA-switch-rev 47 64.0 TCCGGCGTAGAGGATCCAGATCTTACAGGCGTTTGCGGATTGGTTCC
17ho pK18msb_seq_1 20 55.0 TAGCTATCGCCATGTAAGCC
17hp pK18msb_seq_2 20 62.0 AGCGGAAGAGCGCCCAATAC
17hq aaRS_pSB1C3_fw 50 58.0 CGGCCGCTTCTAGAGGATTATCAATTTTAAAAAACTAACAGTTGTCAGCC
17hr aaRS_pSB1C3_rev 39 58.0 GCAGCGGCCGCTACTAGTACTCGAGCATGCAAAAAAGCC
17hs pSB1C3_aaRS_fw 44 65.0 GCTTTTTTGCATGCTCGAGTACTAGTAGCGGCCGCTGCAGTCCG
17ht pSB1C3_aaRS_rev 50 65.0 GTTTTTTAAAATTGATAATCCTCTAGAAGCGGCCGCGAATTCCAGAAATC
17hu sgRNA_romA_1_fw 40 68.0 CGTCAGTCCAGATACCTTTGATGTCCTGATTGGAAGGACC
17hv sgRNA_romA_1_rv 40 68.0 ACTCCTCCTCTCAAGGACTATAGAAAAAAAGTCCCGCTGC
17hw sgRNA_romG_2_fw 40 68.0 GCAGCGGGACTTTTTTTCTATAGTCCTTGAGAGGAGGAGT
17hx sgRNA_romG_2_rv 40 69.0 GCTCACAGGACACCTGGTATACGTATTGATCTCACGAGGC
17hy sgRNA_romd_3_fw 40 69.0 GCCTCGTGAGATCAATACGTATACCAGGTGTCCTGTGAGC
17hz sgRNA_romd_3_rv 40 67.0 GATTATCGGGCAAATACCTTCTCATACGTGACGGATTCCC
17ia sgRNA_romT_4_fw 40 67.0 GGGAATCCGTCACGTATGAGAAGGTATTTGCCCGATAATC
17ib sgRNA_romT_4_rv 40 69.0 ATACGTTCCCTTTAATTGCCTGCCGGAGTTAAGGACGTTC
17ic sgRNA_romC_5_fw 40 69.0 GAACGTCCTTAACTCCGGCAGGCAATTAAAGGGAACGTAT
17id sgRNA_romC_5_rv 40 68.0 GCTAGTATTACTCTGATCTGATGTTTGCAGTCGCTGCTGG
17ie Prefix_rev 17 59.0 CTCTAGAAGCGGCCGCG
17if pSC101-ori-part_gaF 45 57.0 CTGGAATTCGCGGCCGCTTCTAGAGGAGTTATACACAGGGCTGGG
17ig pSC101-ori-part_gaR 49 58.0 CGGACTGCAGCGGCCGCTACTAGTATCAGATCCTTCCGTATTTAGCCAG
17ih sgRNAs_Rom_gaF 66 62.0 GGCCTTTCTGCGTTTATATACTAGAAGCGGCCGCTGCACGTCAGTCCAGATACCTTTGATGTCCTG
17ii sgRNAs_Rom_gaR 78 63.0 AACTAACTAACCCTTAGTGACTCCTGCAGCGGCCGCTACTAGTAGCTAGTATTACTCTGATCTGATGTTTGCAGTCGC
17ij sgRNA_Rom_BB_gF 32 62.0 GCTGCAGGAGTCACTAAGGGTTAGTTAGTTAG
17ik sgRNA_Rom_BB_gR 32 63.0 CCGCTTCTAGTATATAAACGCAGAAAGGCCCA
17il SeqCas1 20 55.0 ATTTGGCCTTAGCGCATATG
17im SeqCas2 20 58.0 GGCTCCCCTATCAGCTTCAA
17in SeqCas3 21 56.0 TCGATAAAGGTGCTTCAGCTC
17io SeqCas4 20 59.0 GGTTGGGGACGTTTGTCTCG
17ip SeqCas5 22 56.0 GGAAGAGACATGTATGTGGACC
17iq SeqCas6 20 60.0 GCCGTCGTTGGAACTGCTTT
17ir SeqCas7 21 56.0 CAGTCCTAGTGGTTGCTAAGG
17is SeqCas8 20 61.0 AATCTTGGAGCTCCCGCTGC
17it Seq_LFS 20 57.0 CGTCCTGATTCAGACCTCCT
17iu Seq_B0015 20 58.0 GGCTCAGTCGAAAGACTGGG
17iv RuBisCo_RFP_fw 56 64.0 GCTAGTGTAGATCGCAAAGAGGAGAAATACTAGATGGCAGTTAAGAAATATAGCGC
17iw Rubisco_RFP_rev 37 64.0 AGCCATTCTCTAGTAGTTGCCGCGGTAGACCACGAAG
17ix RFP_RuBisCo_rev 49 64.0 GTATTTCTCCTCTTTGCGATCTACACTAGCACTATCAGCGTTATTAAGC
17iy RFP_RuBisCo_fw 43 64.0 GGCAACTACTAGAGAATGGCTTCCTCCGAAGACGTTATCAAAG
17iz ori_pSB1C3_fw_66_3 39 66.0 CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG
17ja pSB1C3_ori_rev_66_2 33 66.0 CGGAGCCTATGGAAAAACGCCAGCAACGCGGCC
17jb pSB1C3_ori_fw_65_4 54 65.0 CAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGC
17jc ori_pSB1C3_rev_65_3 57 65.0 GTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGC
17jd J23100_gaF 43 65.0 CTTAGTGACTCGAATTCGCGGCCGCTTCTAGAGTTGACGGCTA
17je J23100_gaR 56 65.0 TAACTAACTAACCCTTAGTGACTCCTGCAGCGGCCGCTACTAGTATATAAACGCAG
17jf sgRNA_BB_gaF 56 65.0 CTGCAAACATCAGATCAGAGTAATACTAGCTACTAGTAGCGGCCGCTGCAGGAGTC
17jg sgRNA_BB_gaR 62 65.0 ATCAGGACATCAAAGGTATCTGGACTGACGTATAAACGCAGAAAGGCCCACCCGAAGGTGAG
17jh retBB_Rom_gaR 38 61.0 CTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGC
17ji retBB_Rom_gaF 44 60.0 ATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCTTCCTC
17jj pSB1C3_aaRS(oT)_fw_66 55 65.0 ATTTTAGAGCCAATTAGAAAGAGATTATAATACTAGTAGCGGCCGCTGCAGTCCG
17jk aaRS(oT)_pSB1C3_rev_58 71 58.0 CCGGACTGCAGCGGCCGCTACTAGTATTATAATCTCTTTCTAATTGGCTCTAAAATCTTTATAAGTTCTTC
17jl ori_pSB1C3_fw_65 56 65.0 TAAAAAGGCCGCGTTGCTGGCGTTGTGACCAAACAGGAAAAAACCGCCCTTAACAT
17jm neu_pSB1C3_ori_fw_58 51 58.0 TACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGG
17jn pSB1C3_ori_rev_65 40 65.0 GCGGTTTTTTCCTGTTTGGTCACAACGCCAGCAACGCGGC
17jo KanR-mod-fwd (80) 80 72.0 ACTAGAGTCACACAGGAAAGATGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCCGCTTGGGTTGAGAGGCTATTC
17jp KanR-mod-rev (80) 80 72.0 CTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGAAGAACTCGTCAAGAAGGCGATAGAAGGCGATGCGC
17jq KanR-TAG1-fwd (73) 73 71.0 AATGCTAGCTACTAGAGTCACACAGGAAAGATGTAGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCCG
17jr KanR-TAG2-fwd (76) 76 71.0 AATGCTAGCTACTAGAGTCACACAGGAAAGATGTAGTAGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCCG
17js KanR-TAG3-fwd (79) 79 71.0 AATGCTAGCTACTAGAGTCACACAGGAAAGATGTAGTAGTAGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCCG
17jt KanR-mod-fwd-short (59) 59 71.0 ACTAGAGTCACACAGGAAAGATGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCC
17ju pSB1C3_sup_fw 70 70.0 GATCAAAGAACAGGAAGAAGAAGTTGACGACGAAGTTTAATACTAGTAGCGGCCGCTGCAGTCCGGCAAA
17jv pSB1C3_sup_rev 68 68.0 GCTGGTTGTTACCCTGGTTAGAGTCAGACATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTA
17jw sup35_121CUA_fw 50 61.0 CCAGGCTGGTTTCCAGCCGCAGCTACAGGGTATGTCTCTGAACGACTTCC
17jx sup35_121CUA_rv 55 61.0 TAGCTGCGGCTGGAAACCAGCCTGGTAACCTTGCAGGTTGTTGTTGTAGTTGAAG
17jy AcF_CUA_fw 54 67.0 CGGACTTAGAATCCGCATGGCAGGGGTTCAAATCCCCTCCGCCGGACCAAATTC
17jz AcF_CUA_rev 50 67.0 CCCTGCCATGCGGATTCTAAGTCCGCCGTTCTGCCCTGCTGAACTACCGC
17ka AB_BB_gaF 33 69.0 TACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAA
17kb AB_BB_gaR 48 69.0 TACTTTCTCCTCTTTGTCGCTAGCATTGTACCTAGGACTGAGCTAGCC
17kc tRNA_heid_fw 53 61.0 GATTTCTGGAATTCGCGGCCGCTTCTAGAGTGTGCTTCTCAAATGCCTGAGGC
17kd tRNA_heid_rev 55 61.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTACATGCAAAAAAGCCTGCTCGTTGAG
17ke BB1C3_tRNA_h_fw 51 62.0 GCCTGCTCAACGAGCAGGCTTTTTTGCATGTACTAGTAGCGGCCGCTGCAG
17kf BB1C3_tRNA_h_re 52 62.0 CAAACTGGCCTCAGGCATTTGAGAAGCACACTCTAGAAGCGGCCGCGAATTC
17kg pSC101-pK18msb_seqF 20 58.0 GTTCCACTGAGCGTCAGACC
17kh pSC101-pK18msb_seqR 20 56.0 AACGCAGGAAAGAACATGTG
17ki TyrRS_pSB1C3_fw 61 63.0 GATTTTAGAGCCAATTAGAAAGAGATTATAACTGCAGTTTCAAACGCTAAATTGCCTGATG
17kj TyrRS_pSB1C3_rev 63 63.0 GTTATACGTTGTTTACGCTTTGAGGAATCCCATATGGACGAATTTGAAATGATAAAGAGAAAC
17kk PtNTT2_gaF_ext 62 60.0 CACTTTATGCTTCCGGCTCGTATAATGGACAAAGAGGAGAAAGTAATGCGCCCGTATCCGAC
17kl PtNTT2_gaR_ext 68 56.0 CTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTTTCTTTGTTGGTTTTCGCTTCC
17km LFS_Col 27 58.0 AATTTTCAGATGTGTTTTATCCGGGAG
17kn RFS_Col 30 58.0 GTGATAAATGGTAATGTTGCTCATATCAGT
17ko PtNTT2_trunc_gaF_v2 46 60.0 TCGTATAATGGACAAAGAGGAGAAAGTAATGGGCGGCAGCACCGTG
17kp pSC101ori_ext_gaF 43 64.0 CCGTAGAAAAGATCAAAGGATCTTCAACGCCCTGAGAAGCCCG
17kq pSC101ori_ext_gaR 47 63.0 CGTAAAAAGGCCGCGTTGCTGGCGTGCATCGCCTGTTTCAGGCTGTC
17kr K80+RBS+T7RNAPre 52 53.0 CGTCTACATTACTAGTTTCTCCTCTTTGTTAGCCCAAAAAACGGGTATGGAG
17ks K80T7RNAPp1 61 53.0 CCATACCCGTTTTTTGGGCTAACAAAGAGGAGAAACTAGTAATGTAGACGATTAACATCGC
17kt K808000+suff 51 63.0 CACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAG
17ku K808000+suf_re 59 63.0 CCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCG
17kv K808000+Term 54 58.0 ATCTTAGAGTCGGACTTCGCGTTCGCGTAACCAGGCATCAAATAAAACGAAAGG
17kw K808000+Term_re 50 58.0 GACTGAGCCTTTCGTTTTATTTGATGCCTGGTTACGCGAACGCGAAGTCC
17kx Pre+K808000 54 56.0 GATTTCTGGAATTCGCGGCCGCTTCTAGAGTTATGACAACTTGACGGCTACATC
17ky Pre+K808000_re 50 56.0 TAGCCGTCAAGTTGTCATAACTCTAGAAGCGGCCGCGAATTCCAGAAATC
17kz T7RNAP_pos107_re 20 58.0 TTGCAGGAACTGGAAGGCTG
17la T7RNAP_pos107_BL 22 58.0 TTCCTGCAATAGATCAAGCCGG
17lc I746909+suff_rev 67 60.0 TTGCCGGACTGCAGCGGCCGCTACTAGTATAATACGACTCACTATAGGGAGATACTAGAGAAAGAGG
17ld Pre+I746909re 52 58.0 GATTTCTGGAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGGCCCACC
17le pre+I746909re_rev 55 58.0 GTGGGCCTTTCTGCGTTTATACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC
17lf Seq_RFS 20 66.0 CGCCTGTGCCGCCCTTCAGA
17lg pTarget_seq_fwd 20 54.0 GCCTTTGAGTGAGCTGATAC
17lh pTarget_seq_rev 20 55.0 CGGCACTGTTGCAAATAGTC
17li I746909+suff 49 60.0 TCTAGTATCTCCCTATAGTGAGTCGTATTATACTAGTAGCGGCCGCTGC
17lj PtNTT2_seq_fwd 23 58.0 CCAGGAAGTGACCAAATTTCTGC
17lk PtNTT2_seq_rev 19 60.0 GCAGGCTCTGAATGGTGCC
17ll pSC101-pK18msb-seq3 20 59.0 GAGGAGCAAACAGCGTTTGC
17lm pSC101-pK18msb-seq4 25 59.0 ATCTGTGCATATGGACAGTTTTCCC
17ln sup35_pSB1C3_fw 69 65.0 GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGATGTCTGACTCTAACCAGGGTAACAACCAGCAGAAC
17lo sup35_pSB1C3_rv 53 65.0 GCCGCTACTAGTATTAAACTTCGTCGTCAACTTCTTCTTCCTGTTCTTTGATC
17lp TyrFS_pSB1C3_fw 53 61.0 CTTTGAGGAATCCCATATGGACGAATTTGAAATGATAAAGAGAAACACATCTG
17lq TyrFS_pSB1C3_rv 67 61.0 GCGTTTGAAACTGCAGTTATAATCTCTTTCTAATTGGCTCTAAAATCTTTATAAGTTCTTCAGCTAC
17lr sgRNA_BB_A_1_gF 53 67.0 TTCTATAGTCCTTGAGAGGAGGAGTTACTAGTAGCGGCCGCTGCAGGAGTCAC
17ls sgRNA_BB_G_2_gF 58 67.0 ATCAATACGTATACCAGGTGTCCTGTGAGCTACTAGTAGCGGCCGCTGCAGGAGTCAC
17lt sgRNA_BB_d_3_gF 58 67.0 CACGTATGAGAAGGTATTTGCCCGATAATCTACTAGTAGCGGCCGCTGCAGGAGTCAC
17lu sgRNA_BB_T_4_gF 58 67.0 AACTCCGGCAGGCAATTAAAGGGAACGTATTACTAGTAGCGGCCGCTGCAGGAGTCAC
17lv RomT_mutA_sense 80 nan TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGAATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCT
17lw RomT_mutA_anti 80 nan AGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATTCTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA
17lx RomT_mutG_sense 80 nan TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGGATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCT
17ly RomT_mutG_anti 80 nan AGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATCCTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA
17lz RomT_mutD_sense 80 nan TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCTT
17ma RomT_mutD_anti 80 nan AAGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATCTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA
17mb RomT_mutT_sense 80 nan TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGTATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCT
17mc RomT_mutT_anti 80 nan AGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATACTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA
17md RomT_mutC_sense 80 nan TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGCATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCT
17me RomT_mutC_anti 80 nan AGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATGCTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA
17mf ownT_mutA_sense 80 nan CTAGCTCAGTCCTAGGTACAGTGCTAGCGATGATAGGCGGACTCTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC
17mg ownT_mutA_anti 80 nan GCCATCTAGTATTTCTCCTCTTTCACTAGTACCGAAGAGTCCGCCTATCATCGCTAGCACTGTACCTAGGACTGAGCTAG
17mh ownT_mutG_sense 80 nan CTAGCTCAGTCCTAGGTACAGTGCTAGCGATGATAGGCGGGCTCTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC
17mi ownT_mutG_anti 80 nan GCCATCTAGTATTTCTCCTCTTTCACTAGTACCGAAGAGCCCGCCTATCATCGCTAGCACTGTACCTAGGACTGAGCTAG
17mj ownT_mutT_sense 80 nan CTAGCTCAGTCCTAGGTACAGTGCTAGCGATGATAGGCGGTCTCTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC
17mk ownT_mutT_anti 80 nan GCCATCTAGTATTTCTCCTCTTTCACTAGTACCGAAGAGACCGCCTATCATCGCTAGCACTGTACCTAGGACTGAGCTAG
17ml ownT_mutC_sense 80 nan CTAGCTCAGTCCTAGGTACAGTGCTAGCGATGATAGGCGGCCTCTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC
17mm ownT_mutC_anti 80 nan GCCATCTAGTATTTCTCCTCTTTCACTAGTACCGAAGAGGCCGCCTATCATCGCTAGCACTGTACCTAGGACTGAGCTAG
17mn pSC101-replace-fwd 45 71.0 TATGAAATTGGTGGTTGATAAGCGAGGCCGCCCGACTGATACGTT
17mo pSC101-replace-rev 55 64.0 GCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGG
17mp pK18msB_split_1 29 66.0 GCAACTACAGCTCAGGCGACAACCATACG
17mq pK18msB_split_2 29 66.0 CGTATGGTTGTCGCCTGAGCTGTAGTTGC
17mr sup_21TGT_fw 50 62.0 CCAGCAGTACTCTCAGAATGGATGTCAACAGCAAGGCAATAATCGTTATC
17ms sup_21TGT_rev 70 63.0 GTTGACATCCATTCTGAGAGTACTGCTGGTAATTCTGCTGGTTGTTACCCTGGTTAGAGTCAGACATCTC
17mt TyrRS_FluoAS_fw 67 63.0 GATTAAAATCCTGGAGCCCATTCGCAAGCGCCTGTAACTGCAGTTTCAAACGCTAAATTGCCTGATG
17mu TyrRS_FluoAS_rv 70 63.0 GATGTGTTACGCTTAATCATCTCGAACTCGTCCATATGGGATTCCTCAAAGCGTAAACAACGTATAACGG
17mv ONBY-out-rev_2 35 63.0 ATGAATCTCCAAGTATATAACGACTGCACCTTTGC
17mw CLRS-fw 40 73.0 GAATTCGCGGCCGCTTCTAGAGTTTTAAAAAACTAACAGT
17mx CLRS-rv 40 80.0 CTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCC
17my PrK_210CTT_fw 41 69.0 CCGAAAGACGAAATCAGCCTTAATTCCGGCAAACCGTTTCG
17mz PrK_210CTT_rv 39 69.0 AAGGCTGATTTCGTCTTTCGGATTCAGCAGAACCTCCAG
17na PrK_456CTT_fw 46 69.0 CTTTAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGG
17nb PrK_456CTT_rv 54 69.0 CAATTTAGCGTTTGAAACTAAAGTTACAGGTTCGTAGAGATCCCGTTATAATAC
17nc AcF_308CTT_fw 46 69.0 CTTCAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGG
17nd AcF_308CTT_rv 54 69.0 CAATTTAGCGTTTGAAACTGAAGTTATAATCTCTTTCTAATTGGCTCTAAAATC
17ne AcF_107GAG_fw 59 69.0 AGTGAGTTCCAGCTTGATAAGGATTATACACTGAATGTCTATAGATTGGCTTTAAAAAC
17nf AcF_107GAG_rv 56 69.0 GTGTATAATCCTTATCAAGCTGGAACTCACTTCCATAAACATATTTTGCCTTTAAC
17ng sup35_121CTA_fw 51 70.0 CAAGCTGGATTCCAGCCTCAACTACAGGGGATGTCGCTTAATGACTTTCAG
17nh sup35_121CTA_rv 49 70.0 CCCCTGTAGTTGAGGCTGGAATCCAGCTTGATAACCTTGCAAATTGTTG
17ni sup35_Histag_rev 73 70.0 TTAATGGTGATGATGGTGGTGATGGTGATGATGAACTTCGTCGTCAACTTCTTCTTCCTGTTCTTTAATTAAC
17nj blocking_L_fw 42 72.0 CGGCCTGCTGACTTTCTCGCCGATCAAAAGGCATTTTGCTAT
17nk blocking_L_rv 37 72.0 GCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCC
17nl blocking_R_fw 72 70.0 GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCG
17nm blocking_R_rv 48 70.0 AAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTAAAAAAAGCACCG
17nn sgRNA_biobrck_F 44 63.0 CTAGCATCTAGTGACTCTAGAGCTAAGCGGCCTGCTGACTTTCT
17no sgRNA_biobrck_R 59 63.0 GAACTAGACAGATTCTGCAGCGGCCGCTACTAGTAAAAAAAGCCTGCTCGTTGAGCAGG
17np FluoAS_308CTT 67 69.0 CATCAGGCAATTTAGCGTTTGAAACTGAAGTTACAGGCGCTTGCGAATGGGCTCCAGGATTTTAATC
17nq Prüf_ori_fw_58 32 58.0 CCTTTTTCTTTAAAACCGAAAAGATTACTTCG
17nr Prüf_ori_rev_58 22 58.0 CGGGGCGTAATTTGATATCGAG
17ns NPA_seq_fwd 19 56.0 CCATTTGGGTGATCTTGGC
17nt NPA_seq_rev 24 55.0 CAAGGATTCTAACTCCTCATACGA
17nu GFP_tag_gaF 55 58.0 GAACAAAAGCTTATTTCTGAAGAGGACTTGCGTAAAGGAGAAGAACTTTTCACTG
17nv GFP_tag_gaR 57 54.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTGTATAGTTCATCCATGCCATG
17nw PtNTT2_tag_gaR 55 56.0 CAAGTCCTCTTCAGAAATAAGCTTTTGTTCTTTTTCTTTGTTGGTTTTCGCTTCC
17nx KanR_fw 54 55.0 AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGATGAGCCATATTCAACGGG
17ny KanR_rev 49 53.0 GCCGGACTGCAGCGGCCGCTACTAGTATTAGAAAAACTCATCGAGCATC
17nz KanR_S133Am 23 63.0 GCGCCGGTTGCATTAGATTCCTG
17oa KanR_S133Am_rev 19 63.0 GCGCAGGAACACTGCCAGC
17ob KanR_S154Am 26 63.0 CAGGCGCAATAGCGAATGAATAACGG
17oc KanR_S154Am_rev 23 65.0 GCCTGAGCGAGACGAAATACGCG
17od pSB1C3_PF_rev 52 60.0 CTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATCTCTAGAAGCGGCCGCG
17oe pSB1C3_SF_fw 54 60.0 TGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATACTAGTAGCGGCCGCTGC
17of T7RNAP_BL_fw 20 62.0 CAGACATTGCCGTCACTGCG
17og T7RNAP_BL_rev 17 61.0 GCCACGAAGACCACGCC
17oh T7_CBS_fw 63 63.0 GACTCACTATAGGGAATACAAGCTACTTGTTCTTTTTGCATACTAGTAGCGGCCGCTGCAGTC
17oi T7_CBS_rev 72 63.0 CAAGTAGCTTGTATTCCCTATAGTGAGTCGTATTACTCTAGTATTAGCTATTCAGATTTGCGATACACCACG
17oj KanR_rev_fw 56 52.0 GGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTAGAAAAACTCATCGAGCATC
17ok KanR_rev_rev 54 52.0 CCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAATGAGCCATATTCAACGG
17ol mutT_flanke1_gaF 50 59.0 TAATGTGAGTTAGCTCACTCATTAGGCACCCTCGATTTGCCAATTGCCGA
17om mutT_flanke1_gaR 55 55.0 GTCGGATGCGGCGAAAACGCCTTATCTGACTGTCTTAAACCTATAAAAAAGGCGC
17on mutT-flanke2_gaF 50 56.0 ATTCTGCGCCTTTTTTATAGGTTTAAGACAGTCAGATAAGGCGTTTTCGC
17oo mutT-flanke2-gaR 49 54.0 CTGCGGACTGGCTTTCTACGTGTTCCGCTTGGCACTTACCATGGTGCTG
17op Cas9only_F 64 58.0 GATTTCTGGAATTCGCGGCCGCTTCTAGAGATGGATAAGAAATACTCAATAGGCTTAGATATCG
17oq Cas9only_R 51 58.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGTCACCTCCTAGCTGACT
17or bb_Cas9only_F 55 67.0 ATTGATTTGAGTCAGCTAGGAGGTGACTGATACTAGTAGCGGCCGCTGCAGTCCG
17os bb_Cas9only_R 60 66.0 ATCTAAGCCTATTGAGTATTTCTTATCCATCTCTAGAAGCGGCCGCGAATTCCAGAAATC
17ot crtI_86TAG_fw 51 68.0 GAACTGCTGCCGGTTACGCCGTAGTACCGCCTGTGTTGGGAGTCAGGGAAG
17ou crtI_86TAG_rev 64 68.0 ACGGCGTAACCGGCAGCAGTTCGACATACTCTTTTAACTGTTTTCCTGCCAGTGCAAACAGTTC
17ov crtI_318TAG_fw 55 68.0 CTATTTTGGTTTGAATCACCATCATTAGCAGCTCGCGCATCACACGGTTTGTTTC
17ow crtI_318TAG_rev 61 68.0 ATGATGGTGATTCAAACCAAAATAGAGCACAAACAGAGAGTTACTCATGCGCTTAGTTTGC
17ox crtI_353TAG_fw 49 68.0 GAGGACTTCTCACTTTATCTGTAGGCGCCCTGTGTCACGGATTCGTCAC
17oy crtI_353TAG_rev 60 68.0 CAGATAAAGTGAGAAGTCCTCTGCGAGGCCATCATGATTAAAAATTTCGTCAATCAGCTC
17oz BB_ovexGA_F 18 52.0 GATTTCTGGAATTCGCGG
17pa BB_ovexGA_R 15 52.0 TTTGCCGGACTGCAG
17pb B0015_ovex_F 54 57.0 ATTGATTTGAGTCAGCTAGGAGGTGACTGACCAGGCATCAAATAAAACGAAAGG
17pc B0015_ovex_R 51 57.0 AATGGTAATGTTGCTCATATCAGTATCTCATATAAACGCAGAAAGGCCCAC
17pd B0015_GA_F 54 57.0 GATTTCTGGAATTCGCGGCCGCTTCTAGAGCCAGGCATCAAATAAAACGAAAGG
17pe B0015_GA_R 51 57.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCAC
17pf Cas9o_ovex_F 68 57.0 TGTGAGCGGATAACAATTTCACACAGGAATACTAGATGGATAAGAAATACTCAATAGGCTTAGATATC
17pg Cas9o_ovex_R 50 56.0 ACTGAGCCTTTCGTTTTATTTGATGCCTGGGTCAGCTAGGAGGTGACTGA
17ph RFS_ovex_F 57 56.0 CACCTTCGGGTGGGCCTTTCTGCGTTTATATGAGATACTGATATGAGCAACATTACC
17pi RFS_GA57_R 60 57.0 TTTGCCGGACTGCAGCGGCCGCTACTAGTACAATAAATGTAATGCGTTATATCTTCCTGA
17pj Seq_crtE1_fw 22 58.0 CTACGAGGATCAGGGGTTTACC
17pk Seq_crtE2_fw 22 58.0 GTGGTTCATACCTATCGCGACC
17pl Cas9o_ovex_R2 50 56.0 ACTGAGCCTTTCGTTTTATTTGATGCCTGGTCAGTCACCTCCTAGCTGAC
17pm 28567c3g1_gF 64 59.0 TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCCTTGGATCCTAAAGTGAAG
17pn 28567c3g1_gR 61 59.0 CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCATTCCCATTCGATGGTTGATGG
17po 27205c6g2i2_gF 63 58.0 TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCTTTAGAATTGCAAGACGG
17pp 27205c6g2i2_gR 67 58.0 CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAGAAGGCCTTTTTCTCATAAGAAATGAG
17pq 24513c0g1_gF 65 58.0 TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCTTCTTCAGCTTCCAATAATG
17pr 24513c0g1i3_gR 73 58.0 CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAAGAGAAAAGTCGTTTTTCATATGAGTATAAACC
17ps 24513c0g1i1_gR 73 58.0 CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAAGAATAGAGACGTTTTTCATATGAGTATAAACC
17pt 27205c6g2i4_gR 63 58.0 CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCATGTAGGGAACATCCAGAAGCTTA
17pu 107595c0g1i1_gF 64 59.0 TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGAACCTCAATTCTCTCCGCCTC
17pv 107595c0g1i1_gR 64 59.0 CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCACTTGTATATCAAGGCATCTCGTCC
17pw 30924c1g1i14_gF 63 58.0 TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGGGTCGTTGAAAAATGAAGG
17px 30924c1g1i14_gR 64 58.0 CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAAACACTAAGCTTGGGATGGTAATC
17py 30983c0g1i12_gF 65 61.0 TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGTGTTCCAAAAAATAGCAGCCC
17pz 30983c0g1i12_gR 57 61.0 CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCACTTGCCGCCCGTTTTCC
17qa 26152c4g1i4_gF 75 59.0 TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAAAACGAAAACAAAATAGAAAGTAACAGTAG
17qb 26152c4g1i4_gR 68 59.0 CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCATTTACCATCCATTTTCCACTGTAAGTAC
17qc 29270c2g2i7_gF 64 59.0 TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAAGGTCAATATCCACAGCAG
17qd 29270c2g2i7_gR 67 59.0 CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCACACAAGCTGCACAACAGCAGCCACTCG
17qe 30924c1g1i5_gR 60 58.0 CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAAAGCAGTTCCATCCGTGTAG
17qf 26152c4g1i8_gF 63 58.0 TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAGAATCAAACTGCTCTTGC
17qg L_rob_pSB1C3_fw_65 52 65.0 CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTGGTCCTGGCCACGGGTGC
17qh L_pSB1C3_rop_rev_65 49 65.0 GCCGCGTTGCTGGCGTTGGTCCTGGCCACGGGTGCGCATGATCGTGCTC
17qi k_rop_pSB1C3_fw_66 53 65.0 CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTGCGGAAGTCAGCGCCCTGC
17qj k_pSB1C3_rop_rev_65 50 65.0 CGGAACATAATGGTGCAGGGCGCTGACTTCCGCAACGCCAGCAACGCGGC
17qk Cas9_EcoRI_F 55 67.0 AGACAATCGTGAGAAGATTGAAAAAATCTTGACTTTTCGCATCCCTTATTATGTT
17ql Cas9_EcoRI_R 50 73.0 TTGCCACGCGCCAATGGACCAACATAATAAGGGATGCGAAAAGTCAAGAT
17qm 28626c0g1i1_gF 65 59.0 TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAGCAGTACAGATTCGTAATCG
17qn 28626c0g1i1_gR 65 58.0 CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAACAGGACTTATTAGAACTGCAATGG
17qo CLRS-fw_OL 50 77.0 TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTTTAAAA
17qp CLRS-rv_OL 50 83.0 CCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATATAAACGC
17qq gRNA_fw_codA 25 0.0 TAGC CACAGTGAACGTCGATGAGA
17qr gRNA_rv_codA 25 0.0 AAAC TCTCATCGACGTTCACTGTG
17qs pSB3K5-UBP-gaF 19 60.0 GCCGCTGCAGGAGTCACTA
17qt pSB3K5-UBP-gaR 22 59.0 CCGCGAATTCGAGTCACTAAGG
17qu mRFP-UBP-gaF 22 59.0 CCCTTAGTGACTCGAATTCGCG
17qv mRFP-Link-ccdB-gaR 43 63.0 AAACCTTAAACTGTGCGGCTGCCGCAGCACCGGTGGAGTGACG
17qw ccdB-Linker-mRFP-gaF 58 60.0 CTCCACCGGTGCTGCGGCAGCCGCACAGTTTAAGGTTTACACCTATAAAAGAGAGAGC
17qx ccdB-UBP_gaR 60 60.0 TAGTGACTCCTGCAGCGGCCGCTACTAGTATTATATTCCCCAGAACATCAGGTTAATGGC
17qy ccdB-UBP_gaF 63 59.0 TAGTGAAAGAGGAGAAATACTAGATGGCTTCAGTTTAAGGTTTACACCTATAAAAGAGAGAGC
17qz mRFP-UBP_gaR 47 62.0 GTGGCTAGCACTGTACCTAGGACTGAGCTAGCACCGGTGGAGTGACG
17ra PtNTT2_bb_gaR 32 59.0 CATTACTTTCTCCTCTTTGTCCATTATACGAG
17rb PtNTT2-noSP_gaF 52 61.0 CTCGTATAATGGACAAAGAGGAGAAAGTAATGCTGCCGGTGAAAAAAGGCAC
17rc CLtRNA_fw 47 72.0 TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTGTGC
17rd CLtRNA_rv 30 72.0 GCCGCTGCAGTCCGGCAAAAAAGGGCAAGG
17re RDL_SELP_fw 50 72.0 TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAGAGGAGG
17rf RDL_SELP_rv 30 72.0 CTTGCCCTTTTTTGCCGGACTGCAGCGGCC
17rg XbaI_RBS_baBL_fw 51 60.0 CTTCTAGAGAAAGAGGAGAAACTAGTAATGGCAGCATAGGTTATCAACACG
17rh SpeI_baBL_rev 45 60.0 GCTACTAGTATTATCTGATTTTTGTAAAGGTCTGATAATGGTCCG
17ri Rubisco_110ATT_fw 46 57.0 CGCGGCAATTAGTAAGATATCAAAGAGGAGAAATACTAGATGGCAG
17rj Rubisco_110ATT_rv 41 57.0 GATATCTTACTAATTGCCGCGGTAGACCACGAAGGCCAGAC
17rk BcuI_R 40 68.0 TCCTCTTTCACAAGAAGCTAGCACTGTACCTAGGACTGAG
17rl BcuI_F 40 66.0 TGCTAGCTTCTTGTGAAAGAGGAGAAATACTAGATGGCTT
17rm MHA_GA_F 60 69.0 ATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGC
17rn MHA_GA_R 70 68.0 CGTATAACGTTACTGGTTTCATCTAGTATTTCTCCTCTTTCACAAGAAGCTAGCACTGTACCTAGGACTG
17ro lacI_BB_R 62 68.0 TACCTAGGACTGAGCTAGCCGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTA
17rp lacI_BB_F 67 69.0 TCTTGTGAAAGAGGAGAAATACTAGATGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGT
17rq fwd_CDD_pSB1C3 56 61.0 AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGATGCAAGCCAAACCATTTCAC
17rr rev_CDD_pSB1C3 71 62.0 CCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATCAATTGAAATTTAAATCTAATGTATCAATTGAATC
17rs Split_RBS_F 45 60.0 TACTAGTGAAAGAGGAGAAATACTAGAGATGGCTTCCTCCGAAGA
17rt Split_RBS_R 46 59.0 TTTCTCCTCTTTCACTAGTATGTGTGAAATTGTTATCCGCTCACAA
17ru PtNTT2-pelB-SP_gaF 65 59.0 GCGGCGGCGGGCCTGCTGCTGCTGGCGGCGCAGCCGGCGATGGCGCTGCCGGTGAAAAAAGGCAC
17rv PtNTT2_bb_pelB-SP_gaR 74 55.0 CAGCAGCAGCAGGCCCGCCGCCGCGGTCGGCAGCAGATATTTCATTACTTTCTCCTCTTTGTCCATTATACGAG
17rw PtNTT2_TAT-SP_gaF 77 56.0 ACCACAGGACTTGCCACTATCGGCGCTGCCAGCATGTTTATGCCAAAGGCCAACGCCCTGCCGGTGAAAAAAGGCAC
17rx PtNTT2_bb_TAT-SP_gaR 86 60.0 GCCGATAGTGGCAAGTCCTGTGGTGGCTTTTAAGAAGCCTCGGCGGTTTATTTGCATTACTTTCTCCTCTTTGTCCATTATACGAG
17ry PtNTT2-only_gaF 43 63.0 CTGGAATTCGCGGCCGCTTCTAGAGATGCGCCCGTATCCGACC
17rz fwd_CDD_SpeI 50 50.0 TGATGAAAAATGTGGCTAATACCAGTGTTTTGAAATGGGGTGGAATATTG
17sa rev_CDD_SpeI 76 76.0 AATATTCCACCCCATTTCAAAACACTGGTATTAGCCACATTTTTCATCACCTTGGTATTTCCAAATTTCTTACCTC
17sb AcF_107GAG2_fw 62 64.0 GATAAGGATTATACACTGAATGTCTATAGATTGGCTTTAAAAACTACCTTAAAAAGAGCAAG
17sc AcF_107GAG2_rv 48 64.0 GACATTCAGTGTATAATCCTTATCAAGCTGGAACTCACTTCCATAAAC
17sd PtNTT2-lacO-gaR 53 55.0 TCGACTGAGCCTTTCGTTTTATTTGTTATTTTTCTTTGTTGGTTTTCGCTTCC
17se lacO-PtNTT2_gaF 50 59.0 AAGCGAAAACCAACAAAGAAAAATAACAAATAAAACGAAAGGCTCAGTCG
17sf lacO-PtNTT2_gaR 54 57.0 ATCTAAGCCTATTGAGTATTTCTTATCCATTTCCTGTGTGAAATTGTTATCCGC
17sg Cas9_gaF 63 56.0 TTGTGAGCGGATAACAATTTCACACAGGAAATGGATAAGAAATACTCAATAGGCTTAGATATC
17sh is_kan_fw 44 61.0 CCAATTAACCAATTCTGAATGATTGAACAAGATGGATTGCACGC
17si is_kan_rev 53 61.0 CATAAACAGTAATACAAGGGGTGTTTCAGAAGAACTCGTCAAGAAGGCGATAG
17sj bb_kan_fv 56 61.0 CGCCTTCTTGACGAGTTCTTCTGAAACACCCCTTGTATTACTGTTTATGTAAGCAG
17sk bb_kan_rv 53 61.0 GCAATCCATCTTGTTCAATCATTCAGAATTGGTTAATTGGTTGTAACACTGGC
17sl pSB_CFP_fw 54 69.0 GTCACTCCACCGGTGCTTAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAG
17sm pSB_YFP_rev 60 69.0 CAGTTCTTCGCCTTTGCTCATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGC
17sn CFP_YFP_fw 50 63.0 GAATTCGCGGCCGCTTCTAGAGATGAGCAAAGGCGAAGAACTGTTTACCG
17so CFP_pSB_rev 64 63.0 GAACCAGCAGCGGAGCCAGCGGATCCCATTTTATACAGTTCATCCATGCCATGGGTAATACCGG
17sp YFP_CFP_fw 67 63.0 GGGATCCGCTGGCTCCGCTGCTGGTTCTGGCGAATAGATGGCTTCCTCCGAAGACGTTATCAAAGAG
17sq YFP_pSB_rev 53 63.0 CCGGACTGCAGCGGCCGCTACTAGTATTAAGCACCGGTGGAGTGACGACCTTC
17sr fwd_CCD_Topo 25 60.0 CCGTTTCTCATCGAATATGCAAGCC
17ss rev_CCD_Topo 26 61.0 GCCCAAAAACCATGAAATCCAAGTGG
17st fwd_pTXB1_backb 21 65.0 TGCATCACGGGAGATGCACTA
17su rev_pTXB1_backb 45 66.0 ATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGG
17sv pC3_YFP_fw 49 69.0 GATGAACTGTATAAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAG
17sw pC3_CFP_rv 51 69.0 GGAGGAAGCCATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGC
17sx YFP_pC3_fw 60 63.0 GATTTCTGGAATTCGCGGCCGCTTCTAGAGATGGCTTCCTCCGAAGACGTTATCAAAGAG
17sy YFP_CFP_rv 52 63.0 CAGAACCAGCAGCGGAGCCAGCGGATCCCATAGCACCGGTGGAGTGACGACC
17sz CFP_YFP_fw 56 63.0 TGGCTCCGCTGCTGGTTCTGGCGAATAGATGAGCAAAGGCGAAGAACTGTTTACCG
17ta CFP_pC3_rev 58 63.0 GGACTGCAGCGGCCGCTACTAGTATTTATACAGTTCATCCATGCCATGGGTAATACCG
17tb B0015_ba_fw 58 63.0 CCATTATCAGACCTTTACAAAAATCAGATAACCAGGCATCAAATAAAACGAAAGGCTC
17tc B0015_ba_r_rev 64 63.0 CGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGGCCCACCCG
17td baBL_fw 67 60.0 CTCCATACCCGTTTTTTGGGCTAACAAAGAGGAGAAACTACTAATGGCAGCATAGGTTATCAACACG
17te baBL_rev 70 60.0 CGACTGAGCCTTTCGTTTTATTTGATGCCTGGTTATCTGATTTTTGTAAAGGTCTGATAATGGTCCGTTG
17tf K80_ba_rev 70 56.0 GATAACCTATGCTGCCATTAGTAGTTTCTCCTCTTTGTTAGCCCAAAAAACGGGTATGGAGAAACAGTAG
17tg K808000_ba_r_rev 60 56.0 CCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATGACAACTTGACGGCTACATC
17th pSB1C3_ba_r_fw 66 63.0 GGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATACTAGTAGCGGCCGCTGCAG
17ti pSB1C3_ba_r_rev 64 63.0 GGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATACTCTAGAAGCGGCCGCGAATTCCAGAAATC
17tj pSB1C3_prom_fw 69 65.0 CCTCTTTGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAATACTAGTAGCGGCCGCTGCAGTCCGGC
17tk Term_kanR_rev 69 54.0 CCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGGCCC
17tl Term_kanR_fw 53 54.0 GCAGTTTCATTTGATGCTCGATGAGTTTTTCTAACCAGGCATCAAATAAAACG
17tm pSB1C3_term_rev 70 65.0 CTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC
17tn kanR_term_rev 71 55.0 GGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGTTAGAAAAACTCATCGAGCATCAAATG
17to kanR_prom_fw 70 55.0 GACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCAAAGAGGAGAAAGTAGTAATGAGCCATATTCAACGGG
17tp SpeI_weg_fw 24 54.0 GAGGAGAAAGTAGTAATGTAGACG
17tq SpeI_weg_rev 21 54.0 CTTTCTCCTCTTTGTTAGCCC
17tr qPCR_mRFP_F 23 62.0 AAGGTCGTCCGTACGAAGGTACC
17ts qPCR_mRFP_R 25 63.0 TCAGCCGGGTGTTTAACGTAAGCTT
17tt Cm_pSB6A1_fw_53 42 53.0 GAGTAAACTTGGTCTGACAGGATTCTCACCAATAAAAAACGC
17tu Cm_pSB6A1_rev_53 46 53.0 GTTAATGTCATGATAATAATGGTTTCTTAGACCGTCAGGTGGCACT
17tv pSB6A1_Cm_fw_52 53 52.0 CCCGATCAACTCGAGTGCCACCTGACGGTCTAAGAAACCATTATTATCATGAC
17tw pSB6A1_Cm_rev_52 54 52.0 GGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCTGTCAGACCAAGTTTACTC
17tx mRFP-UBP-New_gaR 47 62.0 ATCGCTAGCACTGTACCTAGGACTGAGCTAGCACCGGTGGAGTGACG
17ty ccdB-UBP-Max_gaF 64 58.0 ACTAGTGAAAGAGGAGAAATACTAGATGGCTCAGTTTAAGGTTTACACCTATAAAAGAGAGAGC
17tz ccdB-UBP-New_gaF 64 59.0 ATAGTGAAAGAGCAGAAATACTAGATGGCTTCAGTTTAAGGTTTACACCTATAAAAGAGAGAGC
17ua codA_lin_fwd 21 59.0 CATTAAAAGCGGTCGTTCCCG
17ub codA-lin_rev 20 59.0 CGGAATTTGCCACCAGAGAG
17uc mRFP1_CBT-Asp_fw 35 70.0 TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCT
17ud mRFP1_CBT-Asp_rv 26 70.0 CCTTTTTTGCCGGACTGCAGCGGCCG
17ue Cas9_Split_F 32 61.0 ATCTTGACTTTTCGCATCCCTTATTATGTTGG
17uf Cas9_Split_R 32 61.0 CCAACATAATAAGGGATGCGAAAAGTCAAGAT
17ug RBS_tight_F 43 nan TTTCACACAGGAATACTAGATGGCTTCCTCCGAAGACGTTATC
17uh RBS_tight_R 41 nan CATCTAGTATTCCTGTGTGAAATTGTTATCCGCTCACAATT
17ui lacUV5_split_F 35 nan TATGCTTCCGGCTCGTATAATGTGTGAAATTGTGA
17uj lacUV5_split_R 35 nan TCACAATTTCACACATTATACGAGCCGGAAGCATA
17uk RBS_B0034_F 49 nan ACAATTAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATCA
17ul RBS_B0034_R 47 nan CATCTAGTATTTCTCCTCTTTAATTGTTATCCGCTCACAATTTCACA
17um RBS_B0035_F 52 nan AATTATTAAAGAGGAGAATACTAGATGGCTTCCTCCGAAGACGTTATCAAAG
17un RBS_B0035_R 49 nan CATCTAGTATTCTCCTCTTTAATAATTGTTATCCGCTCACAATTTCACA
17uo kan_alt_bb_1 23 64.0 TACTAGTAGCGGCCGCTGCAGTC
17up kan_alt_bb_2 18 63.0 CCCTTCGGGAAGCGTGGC
17uq kan_alt_bb_3 21 63.0 GGTCTGACAGCTCGAGTCCCG
17ur kan_alt_bb_4 24 61.0 CCGTTATTCATTCGTGATTGCGCC
17us kan_alt_bb_5 32 60.0 CACCCCTTGTATTACTGTTTATGTAAGCAGAC
17ut pos_kanRr_fw 53 60.0 AGCCTGCTCAACGAGCAGGCTTTTTTGCATGTATAAACGCAGAAAGGCCCACC
17uu pos_B0015_rev 63 60.0 CACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATATAAACGCAGAAAGGCCCACCC
17uv pos_kanRr_rev 55 60.0 AAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGACGGCTAGCTCAGTCCTAGG
17uw pos_T7RNAP_fw 69 60.0 CTTTGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAATTATGACAACTTGACGGCTACATCATTCAC
17ux pos_tRNA_rev 55 60.0 CACCTTCGGGTGGGCCTTTCTGCGTTTATACATGCAAAAAAGCCTGCTCGTTGAG
17uy pSC_mRFP_fw 55 68.0 GTGCTAGTGTAGATCGCCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAG
17uz pSC_mRFP_rev 67 68.0 CTCCTCTTTCTCTAGTATCTCCCTTTAGTGAGTGTTAATTTACTAGTAGCGGCCGCTGCAGTCCGGC
17va mRFP_pSC_fw 64 63.0 GGGAGATACTAGAGAAAGAGGAGAAATACTAGAGATGGCTTCCTCCGAAGACGTTATCAAAGAG
17vb mRFP_pSC_rev 49 63.0 CGGCCGCTTCTAGAGGCGATCTACACTAGCACTATCAGCGTTATTAAGC
17vc T3Pol_2TAG_fw 55 63.0 ATGTAGTAGATCATCGAAAACATCGAAAAGAATGACTTCTCAGAAATCGAACTGG
17vd T3Pol_2TAG_rev 67 63.0 CGATGTTTTCGATGATCTACTACATCTAGTACTTTCCTGTGTGACTCTAGTATTAGAAAAACTCATC
17ve Bb-pRS_RFP_fw_61 24 61.0 GTTGATGTTGCTGTTGGAGGGATG
17vf Bb-pRS_RFP_rev_61 37 61.0 CAACCTTTGGATTTTCATCCTCTCTTGCTATAAGTTC
17vg mRFP_RS_fw_60 58 60.0 GAACTTATAGCAAGAGAGGATGAAAATCCAAAGGTTGCAATACGCAAACCGCCTCTCC
17vh mRFP_RS_rev_60 50 60.0 CTCCATCCCTCCAACAGCAACATCAACTATAAACGCAGAAAGGCCCACCC
17vi 158_159_162 _fw 55 73 55.0 GAGTATGGAACTTATAGCAAGAGAGGATGAAAATCCAAAGGTTGCTGAAGTTATCTATCCAATAATGATGGTT
17vj 158-159-162 rev 55 77 55.0 CTCCATCCCTCCAACAGCAACATCAACGCCMNNATAATGCAGMNNMNNAACCATCATTATTGGATAGATAACTTCAG
17vk S133_S155_fw_59 74 59.0 GCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCTAATGCAACCGGCGCAG
17vl S155_S133_rev_59 61 59.0 GCGATCGCGTATTTCGTCTCGCTCAGGCGCAATAGCGAATGAATAACGGTTTGGTTGATGC
17vm Prüf_S155_S133_rev_59 18 59.0 CTGCGCCGGTTGCATTAG
17vn T7RNAP_spe_fw_59 63 59.0 CCGTTTTTTGGGCTAACAAAGAGGAGAAAGTAGTAATGTAGACGATTAACATCGCTAAGAACG
17vo T7RBS_Spe_rev_61 62 61.0 CGTTCTTAGCGATGTTAATCGTCTACATTACTACTTTCTCCTCTTTGTTAGCCCAAAAAACG
17vp T7RNAP_107_fw_59 48 59.0 GCCCGACAGCCTTCCAGTTCCTGCAATAGATCAAGCCGGAAGCCGTAG
17vq T7RNAP_107_rev_59 52 59.0 GTGATGTACGCTACGGCTTCCGGCTTGATCTATTGCAGGAACTGGAAGGCTG
17vr Prüf_T7RNAP_107_rev_59 19 59.0 CGGCTTCCGGCTTGATCTA
17vs gBlock_delta_own2_s 18 56.0 GTGAGCGATGCTCCGTTG
17vt UBP_PCR_1C3_fw 21 56.0 CCTTAGCTTTCGCTAAGGATG
17vu UBP_PCR_1C3_rv 19 56.0 CGAATTGCAAGACGCTGTC
17vv Prefix-AG_rev 20 nan CTAGAAGCGGCCGCGAATTC
17vw PtNTT2-AG_gaF 43 nan TTCTGGAATTCGCGGCCGCTTCTAGATGCGCCCGTATCCGACC
17vx codA-flank-part_gaF 46 nan CTGGAATTCGCGGCCGCTTCTAGAGCATTAAAAGCGGTCGTTCCCG
17vy codA-flank-part_gaR 45 nan CGGACTGCAGCGGCCGCTACTAGTACGGAATTTGCCACCAGAGAG
17vz mRFP_sacB_gaR 55 nan CTTGTTTTGCAAACTTTTTGATGTTTGCGGCTGCCGCAGCACCGGTGGAGTGACG
17wa sacB_ccdB-rep_gaF 57 nan CGTCACTCCACCGGTGCTGCGGCAGCCGCAAACATCAAAAAGTTTGCAAAACAAGCA
17wb sacB-ccdB-rep_gaR 63 nan TAGTGACTCCTGCAGCGGCCGCTACTAGTATTATTTGTTAACTGTTAATTGTCCTTGTTCAAG
17wc alt_VR_pSB1C3 21 57.0 CCTGCATAACGCGAAGTAATC
17wd sacB-UBP-Max_gaF 60 nan GTACTAGTGAAAGAGGAGAAATACTAGATGGCTAACATCAAAAAGTTTGCAAAACAAGCA
17we sacB-UBP-New_gaF 60 nan GTATAGTGAAAGAGCAGAAATACTAGATGGCTTAACATCAAAAAGTTTGCAAAACAAGCA
17wf retBB_sgRNA_gF 64 65.0 CGAAAAGCCTGCTCAACGAGCAGGCTTTTTTACTAGAGTTGACGGCTAGCTCAGTCCTAGGTAC
17wg retBB_sgRNA_gR 58 66.0 GCTTAGTTGCATCTAGGTGACGCATCTCTCTCTCTAGAAGCGGCCGCGAATTCGAGTC
17wh mRFP-retBB_o2_f 37 56.0 CTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC
17wi mRFP-retBB_o2_r 31 57.0 CCTATCATCGCTAGCACTGTACCTAGGACTG
17wj Split_pSB3C5_for 26 59.0 TGTATCACATATTCTGCTGACGCACC
17wk Split_pSB3C5_rev 26 59.0 GGTGCGTCAGCAGAATATGTGATACA
17wl Split_SpeI_for 40 61.0 TGCTAGCTGCTTGTGAAAGAGGAGAAATACTAGATGGCTT
17wm Split_SpeI_rev 40 61.0 TCCTCTTTCACAAGCAGCTAGCACTGTACCTAGGACTGAG
17wn lacUV5_Split_F 65 55.0 TATGCTTCCGGCTCGTATAATGGACAAAGAGGAGAAAGTAATGGATAAGAAATACTCAATAGGCT
17wo lacUV5_Split_R 60 53.0 CTCTTTGTCCATTATACGAGCCGGAAGCATAAAGTGTAAATATAAACGCAGAAAGGCCCA
17wp pSB1K3_Split_F 24 59.0 GTAAACTTGGTCTGACAGCTCGAG
17wq pSB1K3_Split_R 24 59.0 CTCGAGCTGTCAGACCAAGTTTAC
17wr lacNEU_F 80 61.0 TCGGCTCGTATAATGTGTGAAATTGTGAGCGGATAACAATTTCACACAAGGAGATTACTAGATGGATAAGAAATACTCAA
17ws lacNEU_R 80 64.0 GTGAAATTGTTATCCGCTCACAATTTCACACATTATACGAGCCGATGATTAATTGTCAACAGCCTGGGGTGCCTAAATTG
17wt lacNEU_BB_F 46 67.0 ACAATTTCACACAAGGAGATTACTAGTAGCGGCCGCTGCAGTCCGG
17wu lacNEU_BB_R 53 69.0 AATTCTCACGCTTTCCAGTCCTCTAGAAGCGGCCGCGAATTCCAGAAATCATC
17wv ColPCR_Mis_F 22 55.0 CCAGGCTGTTGACAATTAATCA
17ww ColPCR_R 18 55.0 CCTTCTACGAGCTGTCCG
17wx ColPCR2_F 20 55.0 CCGGGAGGCATTTACTAGAG
17wy c3_g1_i4_fw 27 58.0 CTTAAAGGTGTTGAACATGGTGAGATG
17wz c2_g1_i4_rv 25 58.0 ACCATGTTCAACACCTTTAAGCTTG
17xa c3g1i4_gBl_gF 68 57.0 TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCATTAGATCCTAAGGTTAAAGATC
17xb c3g1i4_gBl_gR 58 57.0 CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCTCCCACTCAATGGTCGAAG
17xc pSB3C5_split_fwd 20 56.0 GTTTCGCCACCACTGATTTG
17xd pSB3C5_split_rev 20 60.0 ACGCTCAAATCAGTGGTGGC
17xe pSB3C5-SpeI-remo-gaF 50 63.0 TACAGTGCTAGCTCATAGTGAAAGAGGAGAAATACTAGATGGCTTCCTCC
17xf pSB3C5-SpeI-remo_gaR 45 60.0 TAGTATTTCTCCTCTTTCACTATGAGCTAGCACTGTACCTAGGAC
17xg sacB-Test-gaF 43 nan GCGGCAGCCGCAGCAGCCGCAACCCGCGATACCAAAGATACCC
17xh mRFP-Test-gaR 41 nan TGCGGCTGCTGCGGCTGCCGCAGCACCGGTGGAGTGACGAC
17xi sacB-test-gaF 47 nan CGTTCGGACTTCACACGGCCAACATCAAAAAGTTTGCAAAACAAGCA
17xj sacB-test-gaR 46 nan GGCCGTGTGAAGTCCGAACGACCACGCTCACGCTGCTATAAATTTC
17xk pTXB1_GMPSopt_f 59 59.0 TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCGCTGGATCCAAAAG
17xl pTXB1_GMPSopt_r 61 59.0 CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGTTCCCATTCGATTGTCGACGGAG
17xm pTXB1_CDAopt_f 69 59.0 TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAACAATATCGCTTTGTAATTGAAGC
17xn pTXB1_CDAopt_r 68 59.0 CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGGCAGCTTTTGTTAGATGAACAATGAAACAC
17xo phyto_fw 38 56.0 AACGTCTCACTCGATCGATGCAAGCCAAACCATTTCAC
17xp phyto_rv 53 54.0 AACGTCTCACTCAATCGTCAATTGAAATTTAAATCTAATGTATCAATTGAATC
17xq GFP-NPtNTT2_gaF 58 nan GCTCGTATAATGGACAAAGAGGAGAAAGTAATGCGTAAAGGAGAAGAACTTTTCACTG
17xr PtNTT2-NGFP_gaR 49 nan AGTTCTTCTCCTTTACGCATTACTTTCTCCTCTTTGTCCATTATACGAG
17xs GFP-NPtNTT2_gaR 64 nan GTCGGATACGGGCGTGCGGCTGCCGCTGCGGCTGCCGCTTTGTATAGTTCATCCATGCCATGTG
17xt PtNTT2-NGFP_gaF 58 nan GATGAACTATACAAAGCGGCAGCCGCAGCGGCAGCCGCACGCCCGTATCCGACCATTG
17xu PtNTT2_seq2_fwd 20 nan GGCCTGGTGTTTGTGTTTGG
17xv PtNTT2(31-575)-T7-gaF 43 nan TAATACGACTCACTATAGGGAAAGAGGAGAAAGTAATGCTGCC
17xw PtNTT2-T7-gaF 43 nan TAATACGACTCACTATAGGGAAAGAGGAGAAAGTAATGCGCCC
7xx PtNTT2-T7-gaR 47 nan TCTCCTCTTTCCCTATAGTGAGTCGTATTACTCTAGAAGCGGCCGCG
17xy MHA-mRFP-seqR 20 nan ACCTTCGTACGGACGACCTT
17xz CWH-mRFP_seqF 20 nan CGGGTGCTTACAAAACCGAC
17ya CWH-sacB-seqR 21 nan CGTGTAATATGGGAAATGCCG
17yb mRFPretBB_25_gF 25 56.0 GAAAGAGGAGAAATACTAGATGGCT
17yc mRFPretBB_25_gR 21 57.0 AGCACTGTACCTAGGACTGAG
17yd PtNTT2_histag_gaF 48 nan CACCATCACCACCATCATCACCATTAATAATACTAGTAGCGGCCGCTG
17ye PtNTT2_histag_gaR 55 nan ATGGTGATGATGGTGGTGATGGTGATGATGTTTTTCTTTGTTGGTTTTCGCTTCC
17yf Seq_Library_fw 90 57.0 AATGGGGTTAAAGGCAAAATATGTTTATGGAAGTGAATTCCAGCTTGATAAGGATTATACACTGAATGTCTATAGATTGGCTTTAAAAAC
17yg Seq_Library_rev 81 58.0 CTGCCCAGCTGGAGTTGTTGAAGGAAATCCAATAATGGAGATAGCTAAATACTTCCTTGAATATCCTTTAACCATAAAAAG
17yh ColPCR_PtNTT2_F 21 55.0 AAAATGGAAGCGAAAACCAAC
17yi split_kan_fw 43 nan GTGCCCTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTG
17yj split_kan_rv 53 nan TCGTCTTGCAGTTCATTCAGGGCACCGGACAGGTCGGTCTTGACAAAAAGAAC
17yk split_kan_ggü_fw 29 nan GGCGGTAATACGGTTATCCACAGAATCAG
17yl split_kan_ggü_rv 29 nan CTGATTCTGTGGATAACCGTATTACCGCC
17ym Seq_57_fw_B8 89 nan AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNCAGCTTGATAAGGATTATACACTGAATGTC
17yn Seq_58_fw_B11 92 nan AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNGCTTGATAAGGATTATACACTGAATGTCTATAG
17yo Seq_57_fw_B21 89 nan AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNGGATTATACACTGAATGTCTATAGATTGGC
17yp Seq_57_fwd_B3 90 nan AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNCACTGAATGTCTATAGATTGGCTTTAAAAAC
17yq Seq_58_B8_rev 81 nan CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCACAACTCCAGCTGGGCAG
17yr Seq_57_alt_rev 87 nan CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCNGATTTCCTTCAACAACTCCAGCT
17ys Seq_57_B11_rev 91 nan CAAGCAGAAGACGGCATACGAGATGTAGCCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCCATTATTGGATTTCCTTCAACAACTC
17yt Seq_56_B21_rev 93 nan CAAGCAGAAGACGGCATACGAGATCGAAACGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCGGATATTCAAGGAAGTATTTAGCTATCTCC