Difference between revisions of "Team:TNCR Korea/Part Collection"

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<h1> Part Collection </h1>
 
<h1> Part Collection </h1>
  
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<p>Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.</p>
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    <li>BBa_K2496000- FLAG</li>
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<li>BBa_K2496001 = DPP4</li>
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<li>BBa_K2496002= strong recombinant </li>
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While you should put all the characterization information for your parts on the Registry, you are encouraged to explain how all your parts form a collection on this page.
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<li>BBa_K2496003= strong anderson promoter(BBa_J23110)</li>
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<li>BBa_K2496004= moderate anderson promoter(BBa_J23111)</li>
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<li>BBa_K2496005= weak anderson promoter(BBa_J23116)</li>
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<h3>Best Part Collection Special Prize</h3>
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<li>BBa_K2496006= moderate recombinant</li>
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<p>Did your team make a lot of great parts? Is there a team that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection or a collection of awesome pigment parts? Tell the judges you should be evaluated for the Best Parts Collection award! To be eligible for this award, these parts must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts.
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<li>BBa_K2496007= weak recombinant</li>
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If you have a collection of parts you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part numbers to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.</p>
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<p>Three composite plasmid(strong, moderate, weak) was designed to control the expression of DPP4, because DPP4 can induce inflammation.
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<h4>Note</h4>
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Strong recombinant plasmid have most strong expression(1) of DPP4
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<p>This page should list all the parts in the collection your team made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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Moderate recombinant plasmid have moderate expression(0.58) of DPP4
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Weak recombinant plasmid have most weak expression(0.1) of DPP4</p>
  
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<p>TNCR_Korea constituted a total of three recombinant plasmids. Anderson promoter (strong, moderate, weak.) + FLAG + DPP4. There are two Pst1 sites in the DPP4 sequence, so you can not access the desired 3A assembly method from iGEM. So we used infusion ligation method instead.</p>
  
 
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Revision as of 01:49, 28 October 2017

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TNCR_Korea

Part Collection

  • BBa_K2496000- FLAG
  • BBa_K2496001 = DPP4
  • BBa_K2496002= strong recombinant
  • BBa_K2496003= strong anderson promoter(BBa_J23110)
  • BBa_K2496004= moderate anderson promoter(BBa_J23111)
  • BBa_K2496005= weak anderson promoter(BBa_J23116)
  • BBa_K2496006= moderate recombinant
  • BBa_K2496007= weak recombinant

Three composite plasmid(strong, moderate, weak) was designed to control the expression of DPP4, because DPP4 can induce inflammation. Strong recombinant plasmid have most strong expression(1) of DPP4 Moderate recombinant plasmid have moderate expression(0.58) of DPP4 Weak recombinant plasmid have most weak expression(0.1) of DPP4

TNCR_Korea constituted a total of three recombinant plasmids. Anderson promoter (strong, moderate, weak.) + FLAG + DPP4. There are two Pst1 sites in the DPP4 sequence, so you can not access the desired 3A assembly method from iGEM. So we used infusion ligation method instead.