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<td><p><font size="-1">c)</font></p><img src="https://static.igem.org/mediawiki/2017/b/bc/T-Nanjing-China-h2s-9.png" width="400" /><p><font size="-1"></p></p></td> | <td><p><font size="-1">c)</font></p><img src="https://static.igem.org/mediawiki/2017/b/bc/T-Nanjing-China-h2s-9.png" width="400" /><p><font size="-1"></p></p></td> | ||
− | <td><p><font size="-1">a)RFP responsiveness of the detector system.<br /> | + | <td><p><font size="-1">Figure 2.a)RFP responsiveness of the detector system.<br /> |
b) A visible photograph of a).<br /> | b) A visible photograph of a).<br /> | ||
c) Test of selectivity.</font></p></td> | c) Test of selectivity.</font></p></td> |
Revision as of 04:22, 28 October 2017
![](https://static.igem.org/mediawiki/2017/3/31/T-Nanjing-China-title-7.png)
Results
In the part of lab work, we have designed three biosensor sequence and improved an old part, J23000. What's more, all the three design have been demonstrate by us.
![](https://static.igem.org/mediawiki/2017/f/f1/T-Nanjing-China-project-ch2o.png)
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Figure 3. Influence of Formaldehyde Induce Time on Fluorescence Expression ![]() |
Figure4.A photograph of E.coli cells containing the formaldehyde-induced RFP expression plasmid, or without formaldehyde induction, and re-suspended in PBS buffer(pH7.4) |
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It is worth to be mentioned that the team OUC help us demonstrate the result. |
![](https://static.igem.org/mediawiki/2017/c/c7/T-Nanjing-China-project-h2s.png)
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In the experiment, we proved that the sequence worked well and was useful to detect hydrogen sulfide | |
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![]() Figure1.Whole-cell sequence dual-enzyme digestion |
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Figure 2.a)RFP responsiveness of the detector system. |
![](https://static.igem.org/mediawiki/2017/0/09/T-Nanjing-China-project-h2.png)
There is a composite of hydrogen sensor full length sequence. The order of the elements is: HoxA-HoxB-HoxC-HoxJ-terminator-HoxP-EGFP. The sequence of HoxABCJP comes from Ralstonia eutropha H16 megaplasmid pHG1. The hole sequence acts as an hydrogen sensor. When the amount of hydrogen goes to a higher level, Fluorescence intensity increases apparently. | |
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The sequence was a good detecter in the lab work. | |
![]() Figure1. Coomassie Brilliant Blue R-250-stained SDS-Page analysis of recombinant E.coli expressing hoxABCJ-terminator-hoxp-gfp |
![]() Fingure 2. Western blot analysis of recombinant E.coli expressing his-hoxA |
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![]() Figure 3. Influence of H2 concentration on fluorescence expression |