Difference between revisions of "Team:UCopenhagen/InterLab"

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                     <h2 class="section-heading">Sub-projects</h2>
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<p class="lead"><strong>Natural endosymbiosis (our model)</strong> Shortly: what is endosymbiosis?
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<strong> Synthetic endosymbiosis </strong> will have innumerable applications if developed to be the orthogonal system we envision. We have chosen to take a bottom-up approach to it, through the investigation of the following three essential mechanisms.
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                    <p class="lead"> Something about the importance of <p>Interdependency<br> in Nature: How it would be necessary for the final product. </p>
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<strong> Interdependence </strong> between host and endosymbiont is necessary in order for the endosymbiotic relationship to be stable and maintained throughout evolution or, more relevant for synthetic biology, through generations. The relationship should be beneficial and crucial for the host and the endosymbiont alike. Interdependency would thus decrease possible safety concerns: without the dependency relationship, the endosymbiotic relationship will not spread and be maintained in a wild population.
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<strong>Number control</strong> addresses our concern that the endosymbiont will thrive too well in the host, and replicate uncontrollably - thus overwhelming the host system - or be lost in host replications. Thus we strive for a way to maintain a stable number of endosymbionts in the system. A control system that repress endosymbiont reproduction in high concentration/numbers, and allow replication when the concentration is decreased, would address this concern.
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<strong>Protein import</strong> is a common trait observed in naturally occurring endosymbiotic relationships, such as mitochondria and chloroplasts. In these cases a down regulation of protein expression in the symbiont is observed, and the protein expression is instead moved to the cell. Proteins expressed in mitochondria and chloroplasts are transcribed in the nucleus, translated in the cytoplasm and subsequently transported across the cell membranes into the endosymbionts. This mechanism would tighten the relationship between host and symbiont, and would allow endosymbiotic manipulation of precursors produced in the host. </p>
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Revision as of 11:49, 28 October 2017

I N T E R L A B

Introduction

We participated in InterLitab, as we want to be contribute to the scientific progress made through this globe spanning project. In InterLab, 6 test devices are inserted in E.coli D5 α, and the growth and fluorescence is measured.

We used the following plasmids provided by iGEM HQ to transform E.coli:

  • Positive control
  • Negative control
  • Test Device 1: J23101+I13504
  • Test Device 2: J23106+I13504
  • Test Device 3: J23117+I13504
  • Test Device 4: J23101.BCD2.E0040.B0015
  • Test Device 5: J23106.BCD2.E0040.B0015
  • Test Device 6: J23117.BCD2.E0040.B0015

Introduction

Something about the importance of

Interdependency
in Nature: How it would be necessary for the final product.

Find Incell here: