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| | {{IISc-Bangalore}} | | {{IISc-Bangalore}} |
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| | <html> | | <html> |
| − | <p><strong>Results</strong></p> | + | |
| − | <h3><strong>CALIBRATION:</strong></h3> | + | <ol id="inPageNav"> |
| − | <p>Calibration of Version1.1 was done using KMnO</span>4 </span>and milk solutions of different dilutions. V1.1 can hold a test tube containing the sample and measure its optical density (OD). The same electronic circuitry was used to measure the OD values of the sample placed in a cuvette. The OD values of the sample was taken using a spectrophotometer (actual) and the device-V1.1 (measured).</span></p> | + | <li><a href="#calibration"> Calibration </a></li> |
| − | <p>KMnO</span>4</span>: Optical Density readings measured from a cuvette, using the device(V1.1) and a spectrophotometer.</span></p> | + | <li><a href="#growthcurves"> Growth Curves </a></li> |
| − | <table>
| + | </ol> |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td colspan="4">
| + | <div id="contentMain"> |
| − | <p>Cuvette</span></p>
| + | |
| − | </td>
| + | <h1 id = "calibration">Calibration</h1> |
| − | </tr>
| + | |
| − | <tr>
| + | <p>Calibration of Version1.1 was done using KMnO4 and milk solutions of different dilutions. V1.1 can hold a test tube containing the sample and measure its optical density (OD). The same electronic circuitry was used to measure the OD values of the sample placed in a cuvette. The OD values of the sample was taken using a spectrophotometer (actual) and the device-V1.1 (measured). |
| − | <td>
| + | </p> |
| − | <p>Substance</span></p>
| + | |
| − | </td>
| + | <p>The Mini (then called Version 1.1) was calibrated against a spectrophotometer (Shimadzu UV-1800) at 648 nm by running serial dilutions of KMnO4 solutions and milk. |
| − | <td>
| + | </p> |
| − | <p>Signal</span></p>
| + | |
| − | </td>
| + | <img src="https://static.igem.org/mediawiki/2017/f/ff/T--IISc-Bangalore--ecoli2.jpg" align ="center"> |
| − | <td>
| + | |
| − | <p>OD(Actual)</span></p>
| + | <p>There has to be a better way. That way is GCODe.</p> |
| − | </td>
| + | |
| − | <td>
| + | <p>GCODe (Growth Curve and Optical DEnsity) is an optical density measurement device that will not only take readings at pre-programmed intervals or continuously, but will also aerate and dilute the culture as required. It can even send you a message when the OD reaches a particular level, just in time to you to start the next stage of your experiment. Fundamentally, it automates the grunt work of growth curves, in a manner that allows you to walk away from the lab, secure in the knowledge that you will be alerted when your cells are ready for you.</p> |
| − | <p>OD(Measured)</span></p>
| + | |
| − | </td>
| + | <p>Our work stemmed from our own frustrations running growth curves night after night, and was also inspired by the OD meter built by the 2014 Aachen iGEM team. We worked incrementally, starting with a rudimentary box of wiring, designing version after version in response to feedback from the iGEM wetlab team, as they trialled our device, and other labs and professors from across IISc.</p> |
| − | </tr>
| + | |
| − | <tr>
| + | <p>We currently have two versions of GCODe - The GCODE Mini and the GCODe Pro. |
| − | <td>
| + | |
| − | <p>Water</span></p>
| + | The GCODe Mini has been developed, fabricated, tested and fully documented. The GCODe Pro is still under development and is in its third version. |
| − | </td>
| + | |
| − | <td>
| + | </p> |
| − | <p>656</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0</span></p>
| + | <h1 id="growthcurves">Growth Curves</h1> |
| − | </td>
| + | |
| − | <td>
| + | <h2>Intro</h2> |
| − | <p>0</span></p>
| + | |
| − | </td>
| + | <p>The GCODe Mini is a sleek black acrylic cuboid that houses a test tube that will hold your culture. Connect the Mini to your laptop via USB, tell it what to do with our associated software, place it in the shaker-incubator, press Start … aaand you’re done !</p> |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td colspan="3">
| + | |
| − | <p>KMnO4 Dilutions</span></p>
| + | </div> |
| − | </td>
| + | |
| − | <td> </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.1</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>492</span></p>
| + | |
| − | </td>
| + | |
| − | <td> | + | |
| − | <p>0.112</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.12</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.2</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>365</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.219</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.25</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.3</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>277</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.328</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.37</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.4</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>212</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.44</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.49</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.5</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>163</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.544</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.6</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.6</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>126</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.656</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.72</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.7</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>99</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.766</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.82</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.8</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>80</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.876</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.91</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>~0.9</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>64</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.999</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>1.01</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>~1</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>51</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>1.137</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>1.11</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table>
| + | |
| − | <p><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /></p>
| + | |
| − | <p>KMnO</span>4</span>: test tube readings</span></p>
| + | |
| − | <p> </p>
| + | |
| − | <table>
| + | |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td colspan="4">
| + | |
| − | <p>V1.1</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>Substance</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>Signal</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>OD(Actual)</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>OD(Measured)</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>Water</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>550</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td colspan="3">
| + | |
| − | <p>KMnO4 Dilutions</span></p>
| + | |
| − | </td>
| + | |
| − | <td> </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.1</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>355</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.112</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.19</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.2</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>237</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.219</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.37</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.3</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>159</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.328</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.54</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.4</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>109</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.44</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.7</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.5</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>78</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.544</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.85</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.6</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>56</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.656</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.99</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.7</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>42</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.766</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>1.12</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.8</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>34</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.876</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>1.21</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>~0.9</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>27</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.999</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>1.31</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>~1</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>23</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>1.137</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>1.38</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table>
| + | |
| − | <p><br /><br /><br /><br /><br /></p>
| + | |
| − | <p>�</span></p>
| + | |
| − | <p>Milk: Cuvette readings</span></p>
| + | |
| − | <table>
| + | |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td colspan="4">
| + | |
| − | <p>Cuvette</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>Substance</span></p> | + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>Signal</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>OD(Actual)</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>OD(Measured)</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>Water</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>627</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>Milk Dilutions</span></p>
| + | |
| − | </td>
| + | |
| − | <td> </td>
| + | |
| − | <td> </td>
| + | |
| − | <td> </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.1</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>549</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.092</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.06</span></p> | + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.2</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>483</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.186</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.11</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.3</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>406</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.301</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.19</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.4</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>359</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.406</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.24</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.5</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>315</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.485</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.3</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.6</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>277</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.598</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.35</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.7</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>252</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.689</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.4</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.8</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>225</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.772</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.45</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.9</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>197</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.879</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.5</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>1.0</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>179</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>1.007</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.54</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table>
| + | |
| − | <p><br /><br /><br /></p>
| + | |
| − | <p>�</span></p>
| + | |
| − | <p>Milk: test tube (V1.1) readings</span></p>
| + | |
| − | <table>
| + | |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td colspan="4">
| + | |
| − | <p>V1.1</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>Substance</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>Signal</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>OD(Actual)</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>OD(Measured)</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>Water</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>554</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>Milk Dilutions</span></p>
| + | |
| − | </td>
| + | |
| − | <td> </td>
| + | |
| − | <td> </td>
| + | |
| − | <td> </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.1</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>452</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.092</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.09</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.2</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>375</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.186</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.17</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.3</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>299</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.301</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.27</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.4</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>244</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.406</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.36</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.5</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>206</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.485</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.43</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.6</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>168</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.598</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.52</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.7</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>143</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.689</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.59</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.8</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>122</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.772</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.66</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0.9</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>102</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.879</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.73</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>1.0</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>83</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>1.007</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.82</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table>
| + | |
| − | <p><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /></p>
| + | |
| − | <h1><strong>Growth Curves</strong></h1>
| + | |
| − | <p> </p>
| + | |
| − | <p>Growth curves of </span><em>Salmonella typhimurium</span></em> (</span>STMwt14028) </span>and </span><em>Escherichia coli</span></em> were made. The optical densities were simultaneously measured using a spectrophotometer at wavelength 600nm and our device GCODe mini.</span></p>
| + | |
| − | <p>According to the Beer-Lambert’s law, we expect that the path length and epsilon to be different for our optical system as compared to the spectrophotometer. However, the concentration will still be the same in both cases. Hence, we expect the OD to be proportional. </span></p>
| + | |
| − | <p>Beer-Lambert’s Law: </span>A=</span>c</span>l</span></p>
| + | |
| − | <p>where, A= Absorbance, </span>= Absorbance, c=concentration, </span>l</span>=path length of the light</span></p> | + | |
| − | <p>GCODe mini: </span>A</span>m</span>=</span>m</span>c</span>l</span>m</span></p>
| + | |
| − | <p>Spectrophotometer: </span>A</span>s</span>=</span>s</span>c</span>l</span>s</span></p> | + | |
| − | <p>A</span>m</span>A</span>s</span></p>
| + | |
| − | <p>In the following graphs, we have scaled the GCODe mini readings with the 12hr and the 24hr readings of the spectrophotometer.</span></p>
| + | |
| − | <p><br /><br /></p>
| + | |
| − | <p>�</span></p>
| + | |
| − | <p> </p>
| + | |
| − | <table>
| + | |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td colspan="7">
| + | |
| − | <p>24H Curve 1 STMwt14028</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>Time</span></p>
| + | |
| − | <p>(hours)</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>OD600 (Spectrophotometer)</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>V1 Reading</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>V1OD</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>OD600</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>V1OD_N_12H</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>V1OD_N_24H</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.0593</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>552</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.02</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.0122</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.009</span></p>
| + | |
| − | </td> | + | |
| − | <td>
| + | |
| − | <p>0.009</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>3</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.2091</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>293</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.27</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.1121</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.124</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.115</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>6</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.3105</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>175</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.49</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.2135</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.226</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.209</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>9</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.3687</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>142</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.58</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.2717</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.267</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.247</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>12</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.4054</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>116</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.67</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.3084</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.308</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.286</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>24</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.4422</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>83</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.81</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.3452</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.373</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.345</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table>
| + | |
| − | <p><br /><br /></p>
| + | |
| − | <p>�</span></p>
| + | |
| − | <p> </p>
| + | |
| − | <table>
| + | |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td colspan="7">
| + | |
| − | <p>24H Curve 2 STMwt14028</span></p>
| + | |
| − | </td>
| + | |
| − | </tr> | + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>Time</span></p>
| + | |
| − | <p>(hours)</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>OD600 (Spectrophotometer)</span></p> | + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>V1 Reading</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>V1OD</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>OD600</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>V1OD_N_12H</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>V1OD_N_24H</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.0888</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>547.49</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.03</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.0392</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.017</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.016</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>3</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.2994</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>222.56</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.42</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.2498</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.236</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.22</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>6</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.3919</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>158.64</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.56</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.3423</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.315</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.293</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>9</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.4136</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>128.08</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.66</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.364</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.371</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.346</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>12</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.4711</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>104</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.75</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.4215</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.422</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.393</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>24</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.5579</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>63</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.97</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.5083</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.545</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.508</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table>
| + | |
| − | <p><br /><br /></p>
| + | |
| − | <p>�</span></p>
| + | |
| − | <p> </p>
| + | |
| − | <table>
| + | |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td colspan="7">
| + | |
| − | <p>24H Curve 1 E.coli</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>Time</span></p>
| + | |
| − | <p>(hours)</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>OD600 (Spectrophotometer)</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>V1 Reading</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>V1OD</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>OD600</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>V1OD_N_12H</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>V1OD_N_10H</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.118</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>584.04</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>2</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.173</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>511.48</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.06</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.055</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.1</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.101</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>4</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.437</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>354.87</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.22</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.319</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.367</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.369</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>6</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.633</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>278.55</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.32</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.515</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.534</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.536</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>8</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.706</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>246.26</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.38</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.588</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.634</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.637</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>10</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.805</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>225.58</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.41</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.687</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.684</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.687</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>12</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.869</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>207.19</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.45</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.751</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.751</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.754</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>24.5</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>1.194</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>165.33</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.55</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>1.076</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.918</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.922</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table>
| + | |
| − | <p> </p>
| + | |
| − | <p>�</span></p>
| + | |
| − | <p> </p>
| + | |
| − | <table>
| + | |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td colspan="7">
| + | |
| − | <p>24H Curve 2 E.coli</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>Time</span></p>
| + | |
| − | <p>(hours)</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>OD600 (Spectrophotometer)</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>V1 Reading</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>V1OD</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>OD600</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>V1OD_N_12H</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>V1OD_N_10H</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>0</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.111</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>595.4</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>2</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.183</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>512.38</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.07</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.072</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.117</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.11</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>4</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.444</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>345.93</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.24</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.333</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.399</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.378</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>6</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.66</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>286.98</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.32</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.549</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.533</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.504</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>8</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.687</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>252.75</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.37</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.576</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.616</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.582</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>10</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.772</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>226.86</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.42</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.661</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.699</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.661</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>12</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.86</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>210.33</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.45</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.749</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.749</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.708</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>
| + | |
| − | <p>24.5</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>1.106</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>174.59</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.53</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.995</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.882</span></p>
| + | |
| − | </td>
| + | |
| − | <td>
| + | |
| − | <p>0.834</span></p>
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table>
| + | |
| − | <p><br /><br /></p>
| + | |
| − | <p>Notice that the readings from the Mini form an even smoother curve than those from the spectrophotometer! This is probably because the GCODe readings were taken instantaneously, while it takes time to load the cuvette into the spectrophotometer and take a reading, allowing lots more opportunities for human error.</span></p>
| + | |
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Calibration
Calibration of Version1.1 was done using KMnO4 and milk solutions of different dilutions. V1.1 can hold a test tube containing the sample and measure its optical density (OD). The same electronic circuitry was used to measure the OD values of the sample placed in a cuvette. The OD values of the sample was taken using a spectrophotometer (actual) and the device-V1.1 (measured).
The Mini (then called Version 1.1) was calibrated against a spectrophotometer (Shimadzu UV-1800) at 648 nm by running serial dilutions of KMnO4 solutions and milk.
There has to be a better way. That way is GCODe.
GCODe (Growth Curve and Optical DEnsity) is an optical density measurement device that will not only take readings at pre-programmed intervals or continuously, but will also aerate and dilute the culture as required. It can even send you a message when the OD reaches a particular level, just in time to you to start the next stage of your experiment. Fundamentally, it automates the grunt work of growth curves, in a manner that allows you to walk away from the lab, secure in the knowledge that you will be alerted when your cells are ready for you.
Our work stemmed from our own frustrations running growth curves night after night, and was also inspired by the OD meter built by the 2014 Aachen iGEM team. We worked incrementally, starting with a rudimentary box of wiring, designing version after version in response to feedback from the iGEM wetlab team, as they trialled our device, and other labs and professors from across IISc.
We currently have two versions of GCODe - The GCODE Mini and the GCODe Pro.
The GCODe Mini has been developed, fabricated, tested and fully documented. The GCODe Pro is still under development and is in its third version.
Growth Curves
Intro
The GCODe Mini is a sleek black acrylic cuboid that houses a test tube that will hold your culture. Connect the Mini to your laptop via USB, tell it what to do with our associated software, place it in the shaker-incubator, press Start … aaand you’re done !
