Difference between revisions of "Team:IISc-Bangalore/Hardware/Results"
| Line 26: | Line 26: | ||
<p>Growth curves of Salmonella typhimurium (STMwt14028) and Escherichia Coli were made. The optical densities were simultaneously measured using a spectrophotometer at wavelength 600nm and our device GCODe Mini.</p> | <p>Growth curves of Salmonella typhimurium (STMwt14028) and Escherichia Coli were made. The optical densities were simultaneously measured using a spectrophotometer at wavelength 600nm and our device GCODe Mini.</p> | ||
| − | <img src="https://static.igem.org/mediawiki/2017/d/d4/T--IISc-Bangalore--hardware_gc1.jpg" align =" | + | <img src="https://static.igem.org/mediawiki/2017/d/d4/T--IISc-Bangalore--hardware_gc1.jpg" align ="center" > |
| − | <img src="https://static.igem.org/mediawiki/2017/3/3c/T--IISc-Bangalore--hardware_gc2.jpg" align =" | + | <img src="https://static.igem.org/mediawiki/2017/3/3c/T--IISc-Bangalore--hardware_gc2.jpg" align ="center"> |
<p></p> | <p></p> | ||
| − | <img src="https://static.igem.org/mediawiki/2017/f/f2/T--IISc-Bangalore--ecoli1.jpg" align =" | + | <img src="https://static.igem.org/mediawiki/2017/f/f2/T--IISc-Bangalore--ecoli1.jpg" align ="center"> |
| − | <img src="https://static.igem.org/mediawiki/2017/f/ff/T--IISc-Bangalore--ecoli2.jpg" align =" | + | <img src="https://static.igem.org/mediawiki/2017/f/ff/T--IISc-Bangalore--ecoli2.jpg" align ="center"> |
<p>Notice that the E. Coli readings from the Mini form an even smoother curve than those from the spectrophotometer! This is probably because the GCODe readings were taken instantaneously, while it takes time to load the cuvette into the spectrophotometer and take a reading, allowing lots more opportunities for human error.</p> | <p>Notice that the E. Coli readings from the Mini form an even smoother curve than those from the spectrophotometer! This is probably because the GCODe readings were taken instantaneously, while it takes time to load the cuvette into the spectrophotometer and take a reading, allowing lots more opportunities for human error.</p> | ||
Revision as of 13:14, 28 October 2017
| Sponsors | |||||
|---|---|---|---|---|---|
 |
 |
 |
|||
 |
 |
||||
| Contact | |||||
|---|---|---|---|---|---|
|
|
|
|||
Calibration
Before we could try running growth curves with GCODe, we needed to check that its readings were linear. We calibrated the Mini (then called Version 1.1) against a spectrophotometer (Shimadzu UV-1800) at 648 nm by running serial dilutions of KMnO4 solutions and milk.
As you can see, the graphs are linear to within experimental error! Data from which these graphs were generated is available at [data link]
Growth Curves
With the knowledge that GCODe's readings are linear, we are now ready to run Growth Curves! There is a slight hitch, however. Optical Density is calculated using Beer-Lambert's Law, which states that Absorbance = Absorbance coefficient x Concentration x Path Length. But we realised that the path length and epsilon are different for our optical system as compared to the spectrophotometer. However since the concentration will still be the same in both cases, the OD will be proportional. We can then compare the graphs by normalizing, ie dividing by any one reading.
Growth curves of Salmonella typhimurium (STMwt14028) and Escherichia Coli were made. The optical densities were simultaneously measured using a spectrophotometer at wavelength 600nm and our device GCODe Mini.
Notice that the E. Coli readings from the Mini form an even smoother curve than those from the spectrophotometer! This is probably because the GCODe readings were taken instantaneously, while it takes time to load the cuvette into the spectrophotometer and take a reading, allowing lots more opportunities for human error.
