Difference between revisions of "Team:UNebraska-Lincoln/Design"
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<p>We successfully cloned and transformed E.coli to carry the gene for the enzymes nitrite reductase and vanadium dependent bromoperoxidase. After we accomplished this we began to characterize our various parts. To characterize nitrite reductase we performed the Nessler’s test (put info here on nessler’s test). To characterize the bromoperoxidase we used the monochlorodimedone assay which is commonly used to determine the rate at which the enzyme brominates hydrocarbons. More detailed information on these steps can be found in the <a href="https://2017.igem.org/Team:UNebraska-Lincoln/Notebook">lab notebook</a> and <a href="https://2017.igem.org/Team:UNebraska-Lincoln/Results">results</a> sections. Although we made plans to go further with the experimental design, at this point we ran out of time.</p> | <p>We successfully cloned and transformed E.coli to carry the gene for the enzymes nitrite reductase and vanadium dependent bromoperoxidase. After we accomplished this we began to characterize our various parts. To characterize nitrite reductase we performed the Nessler’s test (put info here on nessler’s test). To characterize the bromoperoxidase we used the monochlorodimedone assay which is commonly used to determine the rate at which the enzyme brominates hydrocarbons. More detailed information on these steps can be found in the <a href="https://2017.igem.org/Team:UNebraska-Lincoln/Notebook">lab notebook</a> and <a href="https://2017.igem.org/Team:UNebraska-Lincoln/Results">results</a> sections. Although we made plans to go further with the experimental design, at this point we ran out of time.</p> | ||
Revision as of 16:04, 28 October 2017
UNL 2017
Helping reduce methane emissions from livestock
Project Design
We started with our brainstorming and project design stage where we thought through various directions we could take our idea, and then we moved into the experimental design stage where we outlined the things we wanted to accomplish over our project's duration.
Brainstorming Stage
Experimental Stage
We successfully cloned and transformed E.coli to carry the gene for the enzymes nitrite reductase and vanadium dependent bromoperoxidase. After we accomplished this we began to characterize our various parts. To characterize nitrite reductase we performed the Nessler’s test (put info here on nessler’s test). To characterize the bromoperoxidase we used the monochlorodimedone assay which is commonly used to determine the rate at which the enzyme brominates hydrocarbons. More detailed information on these steps can be found in the lab notebook and results sections. Although we made plans to go further with the experimental design, at this point we ran out of time.
Woulda, Coulda, Shoulda
Design
Design is the first step in the design-build-test cycle in engineering and synthetic biology. Use this page to describe the process that you used in the design of your parts. You should clearly explain the engineering principles used to design your project.
This page is different to the "Applied Design Award" page. Please see the Applied Design page for more information on how to compete for that award.
What should this page contain?
- Explanation of the engineering principles your team used in your design
- Discussion of the design iterations your team went through
- Experimental plan to test your designs
Inspiration
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