Difference between revisions of "Team:NTHU Taiwan/Contribution"

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<h1 style="color:#DF6A6A">Wet Lab Note
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<p>
 
1. interlab measurement study
 
1. interlab measurement study
 
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Revision as of 21:07, 28 October 2017

HOME
Team
Attributions
Collaborations
Description
Design
Contribution
Model
Results
Demonstrate
Experiments
Protocol
Notebook
InterLab
Basic Parts
Composite Parts
Part Collection
SAFETY
Silver HP
Integrated and Gold
Public Engagement
Applied Design
Integrated Human Practices
Hardware
Model
Software
Criteria
JUDGING FORM

Wet Lab Note

1. interlab measurement study

We have finished the interlab experiments and get lots of results in the page of interlab

2. Contribution of new parts

We have contributed new 12 biobricks for iGEM part registry and submitted 9 parts to iGEM headquarter.(Figure 1)

Figure 1. the list of our new 12 parts

3. Validation of composite parts’ function

We constructed three composite parts and all of their function have been validated:

(1)Part:BBa_K2354010:monobody-GBP HS

Monobody was fused with gold binding protein and linked with 6×his tag. This fused protein can bind to the gold surface by the function of the gold-binding protein, and it and binds to ER-alpha/EDCs complex.

(2)Part:BBa_K2354011:INP-ER-alpha HS

ER-alpha was fused with ice nucleating protein. This fused protein can be anchored to the membrane of E.coli via the function of ice nucleating protein and ER-alpha can bind to EDCs for detection.

(3)Part:BBa_K2354012:HRP-his tag

Horseradish peroxidase is one kind of peroxidase which has heme as a cofactor, and it can degrade EDCs to the less toxic compounds. Moreover, we linked fused protein with 6×his tag for purification.