Difference between revisions of "Team:USP-Brazil/Results/Detection"
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<p>Firstly, we’ve carried out some experiments in order to evaluate the possibility to use this system in our work. For this purpose, two plasmids containing compatible origins of replications and adequate copy number were designed. pETDuet and pRSFDuet plasmids were chosen for the Switch and the Trigger RNA, respectively.</p> | <p>Firstly, we’ve carried out some experiments in order to evaluate the possibility to use this system in our work. For this purpose, two plasmids containing compatible origins of replications and adequate copy number were designed. pETDuet and pRSFDuet plasmids were chosen for the Switch and the Trigger RNA, respectively.</p> | ||
| − | <img src="https://static.igem.org/mediawiki/2017/b/b1/T--USP-Brazil--Detection--Trigger.png" style="width: 50% | + | <img src="https://static.igem.org/mediawiki/2017/b/b1/T--USP-Brazil--Detection--Trigger.png" style="width: 50%> |
[Switch] | [Switch] | ||
[Trigger] | [Trigger] | ||
Revision as of 23:31, 28 October 2017
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Toehold Switch
Description
We decided to use a toehold switch system as a method to simulate RNA-dependent response in our cells. The image below briefly describes how this system works:
[Toehold]In our system, the Trigger RNA represents an overexpressed messenger RNA which could be, for instance, a bacterial transport system related transcript, only expressed when the cell is under certain stress conditions and aimed to increase bacterial load. On the other hand, the Switch RNA represents the part capable of producing the desired effector, which would then be able to eliminate the related pathogen.
Firstly, we’ve carried out some experiments in order to evaluate the possibility to use this system in our work. For this purpose, two plasmids containing compatible origins of replications and adequate copy number were designed. pETDuet and pRSFDuet plasmids were chosen for the Switch and the Trigger RNA, respectively.
