Difference between revisions of "Team:SSTi-SZGD/Parts"

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{{SSTi-SZGD}}
 
{{SSTi-SZGD}}
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<html lang="en">
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<meta charset="UTF-8">
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<meta name="viewport" content="width=device-width,initial-scale=1,minimum-scale=1,maximum-scale=1,user-scalable=no" />
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<meta name="Keywords" content="product,degradation,soil,pesticide,residues"/>
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<meta name="Description" content="A product for the degradation of soil pesticide residues"/>
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<meta name="author" content="Lucky"/>
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<div class="column full_size">
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<meta name="copyright" content="IGEM Team:SSTi-SZGD"/>
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<meta name="revised" content="Ke Zhang,10/26/17"/>
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<h1>Parts</h1>
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<title>SSTi-SZGD---Expression</title>
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<link rel="stylesheet" type="text/css" href="https://2017.igem.org/Team:SSTi-SZGD/css/base?action=raw&ctype=text/css"/>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<link rel="stylesheet" type="text/css" href="https://2017.igem.org/Team:SSTi-SZGD/css/animation?action=raw&ctype=text/css"/>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<link rel="stylesheet" type="text/css" href="https://2017.igem.org/Team:SSTi-SZGD/css/hover?action=raw&ctype=text/css"/>
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<link rel="stylesheet" type="text/css" href="https://2017.igem.org/Team:SSTi-SZGD/css/header?action=raw&ctype=text/css"/>
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<link rel="stylesheet" type="text/css" href="https://2017.igem.org/Team:SSTi-SZGD/css/footer?action=raw&ctype=text/css"/>
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</div>
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<link rel="stylesheet" type="text/css" href="https://2017.igem.org/Team:SSTi-SZGD/css/Parts?action=raw&ctype=text/css"/>
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<script src="https://2017.igem.org/Team:SSTi-SZGD/js/jquery?action=raw&ctype=text/javascript" type="text/javascript" charset="utf-8"></script>
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<script src="https://2017.igem.org/Team:SSTi-SZGD/js/header?action=raw&ctype=text/javascript" type="text/javascript" charset="utf-8"></script>
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<script src="https://2017.igem.org/Team:SSTi-SZGD/js/footer?action=raw&ctype=text/javascript" type="text/javascript" charset="utf-8"></script>
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<script src="https://2017.igem.org/Team:SSTi-SZGD/js/Parts?action=raw&ctype=text/javascript" type="text/javascript" charset="utf-8"></script>
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<div class="column half_size">
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</head>
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<div class="highlight">
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<body>
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<h5>Note</h5>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<!--SSTi-SZGD-->
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</div>
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<div class="SSTi-SZGD" onselectstart="return false;" unselectable="on" style=" -moz-user-select: none;">
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<div class="header a-fadeinT">
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<div class="logo">
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<div class="column half_size">
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<img src="https://static.igem.org/mediawiki/2017/9/9d/SSTi-SZGD_logo.png"/>
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<span>SSTi-SZGD</span>
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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</div>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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</div>
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<!--nav-->
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<nav class="nav">
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<!--nav_fist-->
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<ul>
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<div class="column half_size">
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<!--Home-->
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<li class="Home mainnav">
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<a href="https://2017.igem.org/Team:SSTi-SZGD">
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<p>The information needed to initially create a part on the Registry is:</p>
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<span>Home</span>
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<ul>
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</a>
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<li>Part Name</li>
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</li>
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<li>Part type</li>
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<li>Creator</li>
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<!--Project-->
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<li>Sequence</li>
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<li class="Project mainnav hvr-overline-from-center">
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<li>Short Description (60 characters on what the DNA does)</li>
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<span>Project</span>
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<li>Long Description (Longer description of what the DNA does)</li>
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<!--Project_nav-->
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<li>Design considerations</li>
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<ul class="unify">
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</ul>
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<li>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Description">Description</a>
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<p>
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</li>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<li>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Expression">Expression</a>
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</div>
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</li>
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<li>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Degradation">Degradation</a>
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<div class="column half_size">
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</li>
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<li>
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<h5>Inspiration</h5>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Applied_Design">Applied Design</a>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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</li>
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<li>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/InterLab">InterLab</a>
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<ul>
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</li>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Safety">Safety</a>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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</li>
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</ul>
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<li>
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</div>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Notebook">Notebook</a>
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</li>
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<div class="column full_size">
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<li>
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<h5>Part Table </h5>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Experiments">Experiments</a>
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</li>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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</ul>
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</li>
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<div class="highlight">
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<!--Application-->
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<li class="Application mainnav hvr-overline-from-center">
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</html>
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<span>Application</span>
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<groupparts>iGEM17 SSTi-SZGD</groupparts>
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<ul class="unify">
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<li>
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<html>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Business_Plan">Business Plan</a>
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</div>
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</li>
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</div>
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<li>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Software">Software</a>
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</li>
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<li>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Hardware">Hardware</a>
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</li>
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</ul>
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</li>
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<!--Human Practices-->
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<li class="Human_Practices mainnav hvr-overline-from-center">
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<span>Human Practices</span>
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<!--Human Practices_nav-->
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<ul class="unify">
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<li>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/HP/Silver">Summary</a>
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</li>
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<li>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/HP/Gold_Integrated">Intergrated HP</a>
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</li>
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<li>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/HP/Outreach">Outreach</a>
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</li>
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<li>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Collaborations">Collaboration</a>
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</li>
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</ul>
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</li>
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<!--Achievement-->
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<li class="Achievement mainnav hvr-overline-from-center">
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<span>Achievement</span>
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<!--Achievement_nav-->
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<ul class="unify">
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<li>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Parts">Parts</a>
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</li>
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<li>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Medal">Medal</a>
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</li>
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</ul>
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</li>
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<!--Team-->
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<li class="Team mainnav hvr-overline-from-center">
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<span>Team</span>
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<!--Team_nav-->
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<ul class="unify">
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<li>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Team">Team</a>
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</li>
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<li>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Attributions">Attributions</a>
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</li>
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</ul>
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</li>
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</ul>
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</nav>
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</div>
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</div>
  
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<!--Content-->
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<div class="Content">
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<div class="Basic">
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<p class="Title">Basic Parts</p>
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<p class="SubTitle">Four Basic parts were built this year.</p>
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<p class="section">
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<a href="http://parts.igem.org/Part:BBa_K2244004" target="_blank">(1) BBa_K2244004</a><br/>
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Coding region of mheI, a functional gene encoding a carbendazim (Methyl-1H-Benzimidazol-2-ylcarbamate, or MBC) hydrolyzing esterase that degrades MBC pesticide.<br/>
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<a href="http://parts.igem.org/Part:BBa_K2244005" target="_blank">(2) BBa_K2244005</a><br/>
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Coding region of LEV1, a conjugated gene encoding a fusion protein LexA-VVD.<br/>
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<a href="http://parts.igem.org/Part:BBa_K2244006" target="_blank">(3) BBa_K2244006</a><br/>
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Regulatory sequence ColE promoter, which contains a SOS operator sequence for the binding of LexA protein.<br/>
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<a href="http://parts.igem.org/Part:BBa_K2244003" target="_blank">(4) BBa_K2244003</a><br/>
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A signal peptide and a coding region of opdA. This is an improved part of BBa_K215090 (iGEM Washington 2009), which contains a functional gene (opdA) that encodes organophosphate hydrolase (OPH) to degrade organophosphate pesticides. In order to develop live cell biocatalysts, we fused a TorA signal peptide of E. coli Tat translocase to the N-terminal of opdA to facilitate the protein exportation to periplasmic space of E.coli.
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</p>
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</div>
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<div class="Composite">
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<p class="Title">Composite Parts</p>
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<p class="SubTitle">Three Composite Parts were built this year.</p>
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<p class="section">
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<a href="http://parts.igem.org/Part:BBa_K2244009" target="_blank">(1) BBa_K2244009</a><br/>
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A Light-repressed gene expression device (LightOFF system). LEV1 repressor binds to operator sequence of ColE promoter thus halts the transcription of reporter gene mCherry<br/>
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<a href="http://parts.igem.org/Part:BBa_K2244011" target="_blank">(2) BBa_K2244011</a><br/>
 +
A heterologous proteins exportation component. Once the lightOFF system is activated by light repression, opdA hydrolase is produced and exported to the periplasmic space of E. coli cells. <br/>
 +
<a href="http://parts.igem.org/Part:BBa_K2244010" target="_blank">(3) BBa_K2244010</a><br/>
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A light-regulated biosafety device adapted from the lightOFF system. Once the lightOFF system is activated by light repression, supernova is produced and accumulated in the cells. Subsequent light illumination leads to release of ROS from supernova and causes cell apoptosis.
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</p>
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<div class="img">
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<img src="https://static.igem.org/mediawiki/2017/3/32/Parts_img1.png"/>
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</div>
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</div>
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</div>
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<!--footer-->
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<footer class="footer_box">
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<div class="footer">
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<!--sponsor-->
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<div class="sponsor">
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<img src="https://static.igem.org/mediawiki/2017/9/9d/SSTi-SZGD_logo.png" alt="SSTi-SZGD"/>
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<img src="https://static.igem.org/mediawiki/2017/e/e0/SSTi-SZGD_logo_SSTI.png" alt="SSTI"/>
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<img src="https://static.igem.org/mediawiki/2017/8/8c/SSTi-SZGD_logo_USZ.png" alt="USZ"/>
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<img src="https://static.igem.org/mediawiki/2017/d/d2/SSTi-SZGD_logo_SSTIABD.png" alt="SSTIABD"/>
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<img src="https://static.igem.org/mediawiki/2017/9/95/SSTi-SZGD_logo_PRS.png" alt="PRS"/>
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</div>
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<!--copyright-->
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<div class="copyright">
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<p>
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<span class="left">
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A product&nbsp;for&nbsp;the&nbsp;degradation&nbsp;of&nbsp;soil&nbsp;pesticide&nbsp;residues
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</span>
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<span class="right">
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Copyright&nbsp;&copy;&nbsp;2017&nbsp;Lucky&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;power&nbsp;by&nbsp;&nbsp;iGEM&nbsp;Team&#58;SSTi-SZGD
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</span>
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</p>
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</div>
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</div>
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</footer>
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</body>
 
</html>
 
</html>

Latest revision as of 07:46, 29 October 2017

SSTi-SZGD---Expression

Basic Parts

Four Basic parts were built this year.

(1) BBa_K2244004
Coding region of mheI, a functional gene encoding a carbendazim (Methyl-1H-Benzimidazol-2-ylcarbamate, or MBC) hydrolyzing esterase that degrades MBC pesticide.
(2) BBa_K2244005
Coding region of LEV1, a conjugated gene encoding a fusion protein LexA-VVD.
(3) BBa_K2244006
Regulatory sequence ColE promoter, which contains a SOS operator sequence for the binding of LexA protein.
(4) BBa_K2244003
A signal peptide and a coding region of opdA. This is an improved part of BBa_K215090 (iGEM Washington 2009), which contains a functional gene (opdA) that encodes organophosphate hydrolase (OPH) to degrade organophosphate pesticides. In order to develop live cell biocatalysts, we fused a TorA signal peptide of E. coli Tat translocase to the N-terminal of opdA to facilitate the protein exportation to periplasmic space of E.coli.

Composite Parts

Three Composite Parts were built this year.

(1) BBa_K2244009
A Light-repressed gene expression device (LightOFF system). LEV1 repressor binds to operator sequence of ColE promoter thus halts the transcription of reporter gene mCherry
(2) BBa_K2244011
A heterologous proteins exportation component. Once the lightOFF system is activated by light repression, opdA hydrolase is produced and exported to the periplasmic space of E. coli cells.
(3) BBa_K2244010
A light-regulated biosafety device adapted from the lightOFF system. Once the lightOFF system is activated by light repression, supernova is produced and accumulated in the cells. Subsequent light illumination leads to release of ROS from supernova and causes cell apoptosis.

A product for the degradation of soil pesticide residues Copyright © 2017 Lucky      power by  iGEM Team:SSTi-SZGD