Difference between revisions of "Team:SSTi-SZGD/HP/Gold Integrated"

 
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<meta name="revised" content="Lucky Yang,10/14/17"/>
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<meta name="revised" content="Lucky Yang,10/26/17"/>
 
<title>SSTi-SZGD---HP_Intergrated</title>
 
<title>SSTi-SZGD---HP_Intergrated</title>
 
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<li>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Design">Design</a>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Expression">Expression</a>
 
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Expression">Expression</a>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Applied_Design">Applied Design</a>
 
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<ul class="unify">
 
<ul class="unify">
 
<li>
 
<li>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Applied_Design">Business Plan</a>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/Business_Plan">Business Plan</a>
 
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<ul class="unify">
 
<ul class="unify">
 
<li>
 
<li>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/HP/Summary">Summary</a>
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<a href="https://2017.igem.org/Team:SSTi-SZGD/HP/Silver">Summary</a>
 
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</nav>
 
</nav>
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</div>
 
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<div class="SubNav">
 
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<div>
 
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<a href="#Potential">Potential customer visits</a>
 
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</div>
 
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<div>
 
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<a href="#Leveking">Visit Leveking</a>
 
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<div class="Content">
 
<div class="Content">
 
 
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<!--Potential customer visits-->
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<!--introduction-->
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<div class="content" id="Potential">
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<div class="introduction">
 +
<p>
 +
To find out what exactly our potential customers need and how we could help meeting their needs. This summer we paid a number of visits to village, private garden/orchard, and Biotech company.
 +
</p>
 +
</div>
 +
 +
<!--NanXi Village---Guangdong Province-->
 +
<div class="Village">
 
 
−
<p class="Title">Potential customer visits</p>
+
<p class="Title">NanXi Village---Guangdong Province</p>
 
 
−
<p>
+
<!--First paragraph-->
−
To make sure that our design truly satisfies the needs of potential users in the future, this summer we paid a number of visits to villages, private farm/orchard/gardens, and small size rural cooperative farmland.  
+
<div class="Firstparagraph">
 +
 +
<div class="left">
 +
<p>
 +
We visited NanXi New Agricultural Science and Technology Village, Jieyang City, which is located in the Western region of Guangdong province. Historically local residents grew oranges, sweet potatoes, and sugar canes for living. In recent years they also started growing decorated trees.
 +
</p>
 +
</div>
 +
 +
<div class="right">
 +
<img src="https://static.igem.org/mediawiki/2017/c/cc/SSTi-SZGD_HP_Intergrated_Fparagraph.png"/>
 +
</div>
 +
 +
</div>
 +
 +
<!--Second paragraph-->
 +
<p class="Secondparagraph">
 +
Like thousands of other villages in China, armers in NanXi are accustomed of using heavy loads of chemical fertilizers and pesticides, including various fungicide, herbicide, and insecticide. The direct consequence was the heavily polluted local riverstream and the disappearance of the marine organisms. Since the introduction of ‘New Countryside’ concept, refers to ecological protection alongside economical development, the conditions of local environment have improved dramatically.  
 
</p>
 
</p>
 
 
−
<!--New countryside visit-->
+
<div class="img">
−
<div class="countryside">
+
<img src="https://static.igem.org/mediawiki/2017/7/72/SSTi-SZGD_HP_Intergrated_img.png"/>
 +
</div>
 +
 +
<!--Third paragraph-->
 +
<div class="Thirdparagraph">
 +
 +
<p>
 +
Local government wishes to maintain their ecological improvement outcome. When we explained our project, they showed great interest and invited us to their village. During the visit, we learned value information regards to local situation, farming, and pesticide usage.
 +
</p>
 +
 +
<p>
 +
NanXi village has over 2000 residents, most of them are elders and children, so there is a short of labor force
 +
</p>
 +
<p>
 +
Farmers mainly use organophosphate pesticide, atrazine herbicide and carbendazim fungicide
 +
</p>
 +
<p>
 +
Local government has funds and resources to import new farming technologies
 +
</p>
 +
<p>
 +
Many residents have water spraying system set up in their farms, thanks to the ‘new countryside’ funds.
 +
</p>
 +
 +
</div>
 +
 +
<div class="img">
 +
<img src="https://static.igem.org/mediawiki/2017/4/4d/SSTi-SZGD_HP_Intergrated_img2.png"/>
 +
</div>
 +
 +
<!--Fourth paragraph-->
 +
<div class="Fourthparagraph">
 +
<p>
 +
Our project may help them degrading pesticide residues in soil or on the surface of the leaves or fruits before pick-up. By using their existing water spraying systems, we could add an addition pump specifically for spraying enzyme solution. However, it appears that farmers are not willing to pay for high price enzyme products. After consulting with local officials, we came up with an idea of using whole cell biocatalysts, which is more economical and easily accepted by farmers. Later in the progress of project, we finalized the design of an automatic spraying system based on the existing water spraying system, and added a suicide system in bacteria cells to avoid GMO leaking problem.
 +
</p>
 +
</div>
 +
 +
</div>
 +
 +
<!--Private balcony garden---Shenzhen-->
 +
<div class="balcony">
 +
 +
<p class="Title">Private balcony garden---Shenzhen
 +
</p>
 +
 +
<div class="Top">
 
 
 
<div class="left">
 
<div class="left">
−
+
<p>
−
<p class="SubTitle">New countryside visit</p>
+
We visited a private balcony garden located on the top floor of a commercial building in Shenzhen. The total size is around 300 sqm, and is divided into five sections with different plants growing, i.e. spring onion, lettuce, spinach, melons, etc. Owner is a businessman who is afraid of contaminated food products so decided to grow his own. He is especially concerned with soil quality and would like to know if his produce are ‘green and residue-free”. e collected soil sample and ran tests on HPLC  to detect traces of esticide. Results showed soil samples inevitably contained small races of pesticide residues, despite the owner seldom uses any. The ossible reason is that soil was purchased from farmers or commercial companies, who obtained soil directly from native land that is eavily polluted by pesticides due to a long history of application.  
−
+
−
<p>We visited NanXi New Agricultural Science and Technology Village, Jieyang City, which is located in the Western region of Guangdong province. Historically it is economically underdeveloped, and local residents usually grow oranges, sweet potatoes, and sugar canes. In recently years, with the majority of labor migrated to large industralized cities (a tendency happens in all part of China), they gradually switched to grow decorated trees that require less labor force. Despite the change of farming produce, the tendency to use heavy loads of chemical fertilizers and pesticides, including fungicide, herbicide, insecticide, have not changed. The direct consequence was the heavily polluted local riverstream and disapparence of the local marine animals (pictures). With the introduction of ‘New Countryside’ concept, and the efforts of local government, the condition of the land, riverstream and canals started improving dramatically (Picture).  
+
 
</p>
 
</p>
−
<p>Under this circumstance, the local government hopes to maintain their ecological improvement outcome and continue searching for sustainable method for future farming. They showed great interest in our project and invited us to visit their village. We admired the efforts they made in changing the ecological condition, and learned that they wish to set up a foundation for eco-travel promotion in the village. Eco-travel means that tourists can enjoy a pure and native environment, playing in the rivel without worry about pollution, picking up vege or fruits from the soil without worry about residues. It also means that their produce can be sold in the market as green and residue-free. In this visit, we introduced our project briefly and asked some farmers about basic practices. Inquiries such as the type of pesticide being used, the amount of being used, as well as chemical fertilizers or bio-fertilizer to probe the farming scenarios.
 
−
</p>
 
−
 
 
</div>
 
</div>
 
 
 
<div class="right">
 
<div class="right">
−
<img src="https://static.igem.org/mediawiki/2017/b/bc/SSTi-SZGD_HP_Intergrated_Potential_countryside.jpeg"/>
+
<img src="https://static.igem.org/mediawiki/2017/6/60/SSTi-SZGD_HP_Intergrated_balconyimg.png"/>
−
<span></span><span></span>
+
−
<img src="img/HP_Intergrated_Potential_countryside2.jpg"/>
+
−
<img src="img/HP_Intergrated_Potential_countryside3.jpg"/>
+
 
</div>
 
</div>
 
 
 
</div>
 
</div>
 +
 +
<div class="Bottom">
 
 
−
<!--Private garder/Farm visits-->
+
<div class="left">
−
<div class="Private">
+
<p>
 +
The owner was happy to try new methods to keep his precious garden “pollution & residue free”, and was little conscious of product price. He showed interest in enzyme products that degrade specific pesticide detected in his garden. He also spread the news on his Wechat and drew interested customers to us in the following weeks.
 +
</p>
 +
</div>
 +
 +
<div class="right">
 +
<img src="https://static.igem.org/mediawiki/2017/9/93/SSTi-SZGD_HP_Intergrated_balconyimg2.png"/>
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
 +
<!--Private Orchard---Shenzhen-->
 +
<div class="Orchard">
 +
 +
<p class="Title">Private Orchard---Shenzhen</p>
 +
 +
<div class="Top">
 
 
 
<div class="left">
 
<div class="left">
−
+
<p>
−
<p class="SubTitle">Private garder/Farm visits</p>
+
Family friends of Joyce own a 16 hectra private orchard in Shenzhen. Besides personal consumption, owners also grow commercialized produces, i.e. dragon fruit, lynchee, longan and papaya, primarily distribute as gift to family, friends or clients. They used heavy loads to fertilizer and pesticides, and seemed little concerned with the potential harm associated with them. However, soil quality and condition is less desirable .  
−
+
−
<p>We visited three private gardens or farm in Shenzhen region this summer. Sizes range from 300 sqm to 16 hectares. Apart from growing vege/fruits for personal consumption, owners also grow commercialized produces, i.e. dragon fruit, lynchee, longan and papaya, for both personal consumption and gifts to friends, family or business clients. They are very conscious of produce and soil quality, but are little concerned of the product appearance. They always refer their produce as 'green, not polluted, eat from the soil'. Therefore, these owners are usually interested in our product concept, and less conscious of price issue. We got four soil testing appointments in two week time since started promoting our project. Test results showed soil samples inevitably contained small amount of pesticide residues, despite they hardly used any. The possible reason is that they purchased soil from farmers or commercial companies, who obtained soil directly from native land that is heavily polluted by pesticides due to a long history of application.
+
 
</p>
 
</p>
−
 
 
</div>
 
</div>
 
 
 
<div class="right">
 
<div class="right">
−
<img src="img/HP_Intergrated_Potential_Private.jpg"/>
+
<img src="https://static.igem.org/mediawiki/2017/4/4e/SSTi-SZGD_HP_Intergrated_Orchardimg.png"/>
−
<img src="img/HP_Intergrated_Potential_Private2.jpg"/>
+
−
<img src="img/HP_Intergrated_Potential_Private3.jpg"/>
+
−
<img src="img/HP_Intergrated_Potential_Private4.jpg"/>
+
 
</div>
 
</div>
 
 
 
</div>
 
</div>
 +
 +
<div class="Bottom">
 
 
 +
<div class="left">
 +
<img src="https://static.igem.org/mediawiki/2017/3/3e/SSTi-SZGD_HP_Intergrated_Orchardimg2.png"/>
 +
</div>
 +
 +
<div class="right">
 +
<p>
 +
They showed some interest in our products, while being price concerned.
 +
Enzyme products might be the primary choice for them, while whole cell
 +
product with automatic spraying system could be an alternative, as they
 +
consider setting up automatic watering system in the orchard soon.
 +
</p>
 +
</div>
 +
 +
</div>
 +
 
</div>
 
</div>
 
 
−
<!--Visit Leveking-->
+
<!--Leveking Bio-tech Co.---Shenzhen-->
−
<div class="content Leveking" id="Leveking">
+
<div class="Leveking">
 
 
−
<p class="Title">Visit Leveking</p>
+
<p class="Title">Leveking Bio-tech Co.---Shenzhen</p>
 
 
−
<div class="left">
+
<div class="Top">
−
<p>
+
−
ShenZhen Leveking Biotech. Co. is a leading supplier of lipase products in Southern China. The company owns a 10,000 sqms manufacturing plant and produces more than 100 types of lipase products each year. Their manufacturing plant has a strong R&D base and mainly uses genetically engineered organisms for production. We would like to form potential business collaboration with them and also like to find out how feasible our project was in terms of commercialization. They provided us with a number parameters related to manufacturing enzyme products, such as chassis choice, enzymatic activity requirement, additive selection, mass production bottlenecks, etc. It appears that although our light-controlled expression system is a valuable alternative to traditional chemical induction, the system still needs quite a few of optimization before implementation. Also, our chassis choice, E.coli., might not be suitable for manufacturing purpose as prokaryotic system has coherent disadvantages to produce functional enzymes compared to eukaryotic system. We were suggested to use Pichia Pastoris or Aspergillus as chassis to help increasing production efficiency. In addition, the large size of our light-off vector (~6kb) could make it difficult for E. coli. to maintain normal growth and expression, which we have encountered frequently in the experimental process ourselves. A change of chassis may help overcome this problem. We took their advice and hope to incorporate into our future project.
+
<div class="left">
−
</p>
+
<p>
 +
ShenZhen Leveking Biotech. Co. is a leading supplier of lipase products in Southern China. The company owns a 10,000 sqms manufacturing plant and manufactures more than 100 types of lipase products each year. Their industrial production is also strongly R&D based and mainly uses genetically engineered organisms for production.  
 +
</p>
 +
</div>
 +
 +
<div class="right">
 +
<img src="https://static.igem.org/mediawiki/2017/9/99/SSTi-SZGD_HP_Intergrated_Levekingimg.png"/>
 +
</div>
 +
 
</div>
 
</div>
 
 
−
<div class="right">
+
<div class="Middle">
−
<img src="img/HP_Intergrated_Leveking.jpg"/>
+
−
<span></span><span></span>
+
<div class="left">
−
<img src="img/HP_Intergrated_Leveking2.jpg"/>
+
<img src="https://static.igem.org/mediawiki/2017/7/7f/SSTi-SZGD_HP_Intergrated_Levekingimg2.png"/>
 +
</div>
 +
 +
<div class="right">
 +
<p>
 +
We visited them for technique supports and industrial production advice. They provided invaluable advice on the feasibility of commercialization of our enzyme products:
 +
<br />
 +
1. Chassis choice---E.coli as being ‘too weak, too simple’ for large-scale production, yeast (Pichia Pastoris) or mold (Aspergillus) is a better choice. Eukaryotic cells also help with large vector (~6kb) expression problem.
 +
<br />
 +
2. Enzymatic activity requirement for industrial production---our current enzyme activity is low for commercialization, and other methods for testing enzyme activity were suggested
 +
<br />
 +
3. Additive selection for enzyme product formulation---a list of additives were provided, such as starch and dextrin
 +
<br />
 +
4. Inducible gene expression system---our LightOFF system is a valuable alternative to chemical induction, however its efficiency and stability needs further verification. Other cheaper chemical inducers also available.
 +
</p>
 +
</div>
 +
 +
</div>
 +
 +
<div class="Bottom">
 +
 +
<div class="left">
 +
<img src="https://static.igem.org/mediawiki/2017/f/fb/SSTi-SZGD_HP_Intergrated_Levekingimg3.png"/>
 +
</div>
 +
 +
<div class="right">
 +
<p>
 +
Being a giant provider in enzyme product, Leveking also suggested a potential business collaboration opportunity with them, such as production outsourcing, which was a bonus to us. It gave us some ideas on how to reduce initial nvestment for our start-up business (refer to <a href="https://2017.igem.org/Team:SSTi-SZGD/Business_Plan">Business plan</a>) and it is reflected in our business plan.
 +
</p>
 +
</div>
 +
 
</div>
 
</div>
 
 
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<!--footer-->
 
<!--footer-->
−
<div class="footer_box">
+
<footer class="footer_box">
 
 
 
<div class="footer">
 
<div class="footer">
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<!--sponsor-->
 
<!--sponsor-->
 
<div class="sponsor">
 
<div class="sponsor">
−
<img src="https://static.igem.org/mediawiki/2017/9/9d/SSTi-SZGD_logo.png"/>
+
<img src="https://static.igem.org/mediawiki/2017/9/9d/SSTi-SZGD_logo.png" alt="SSTi-SZGD"/>
−
<img src="https://static.igem.org/mediawiki/2017/e/e0/SSTi-SZGD_logo_SSTI.png"/>
+
<img src="https://static.igem.org/mediawiki/2017/e/e0/SSTi-SZGD_logo_SSTI.png" alt="SSTI"/>
 +
<img src="https://static.igem.org/mediawiki/2017/8/8c/SSTi-SZGD_logo_USZ.png" alt="USZ"/>
 +
<img src="https://static.igem.org/mediawiki/2017/d/d2/SSTi-SZGD_logo_SSTIABD.png" alt="SSTIABD"/>
 +
<img src="https://static.igem.org/mediawiki/2017/9/95/SSTi-SZGD_logo_PRS.png" alt="PRS"/>
 
</div>
 
</div>
 
 
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<div class="copyright">
 
<div class="copyright">
 
<p>
 
<p>
−
<span class="left">A&nbsp;product&nbsp;for&nbsp;the&nbsp;degradation&nbsp;of&nbsp;soil&nbsp;pesticide&nbsp;residues</span>
+
<span class="left">
−
<span class="right">Copyright&nbsp;&copy;&nbsp;2017&nbsp;Lucky&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;power&nbsp;by&nbsp;&nbsp;IGEM&nbsp;Team&#58;SSTi-SZGD</span>
+
A product&nbsp;for&nbsp;the&nbsp;degradation&nbsp;of&nbsp;soil&nbsp;pesticide&nbsp;residues
 +
</span>
 +
<span class="right">
 +
Copyright&nbsp;&copy;&nbsp;2017&nbsp;Lucky&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;power&nbsp;by&nbsp;&nbsp;iGEM&nbsp;Team&#58;SSTi-SZGD
 +
</span>
 
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</html>
 
</html>

Latest revision as of 09:58, 29 October 2017

SSTi-SZGD---HP_Intergrated

To find out what exactly our potential customers need and how we could help meeting their needs. This summer we paid a number of visits to village, private garden/orchard, and Biotech company.

NanXi Village---Guangdong Province

We visited NanXi New Agricultural Science and Technology Village, Jieyang City, which is located in the Western region of Guangdong province. Historically local residents grew oranges, sweet potatoes, and sugar canes for living. In recent years they also started growing decorated trees.

Like thousands of other villages in China, armers in NanXi are accustomed of using heavy loads of chemical fertilizers and pesticides, including various fungicide, herbicide, and insecticide. The direct consequence was the heavily polluted local riverstream and the disappearance of the marine organisms. Since the introduction of ‘New Countryside’ concept, refers to ecological protection alongside economical development, the conditions of local environment have improved dramatically.

Local government wishes to maintain their ecological improvement outcome. When we explained our project, they showed great interest and invited us to their village. During the visit, we learned value information regards to local situation, farming, and pesticide usage.

NanXi village has over 2000 residents, most of them are elders and children, so there is a short of labor force

Farmers mainly use organophosphate pesticide, atrazine herbicide and carbendazim fungicide

Local government has funds and resources to import new farming technologies

Many residents have water spraying system set up in their farms, thanks to the ‘new countryside’ funds.

Our project may help them degrading pesticide residues in soil or on the surface of the leaves or fruits before pick-up. By using their existing water spraying systems, we could add an addition pump specifically for spraying enzyme solution. However, it appears that farmers are not willing to pay for high price enzyme products. After consulting with local officials, we came up with an idea of using whole cell biocatalysts, which is more economical and easily accepted by farmers. Later in the progress of project, we finalized the design of an automatic spraying system based on the existing water spraying system, and added a suicide system in bacteria cells to avoid GMO leaking problem.

Private balcony garden---Shenzhen

We visited a private balcony garden located on the top floor of a commercial building in Shenzhen. The total size is around 300 sqm, and is divided into five sections with different plants growing, i.e. spring onion, lettuce, spinach, melons, etc. Owner is a businessman who is afraid of contaminated food products so decided to grow his own. He is especially concerned with soil quality and would like to know if his produce are ‘green and residue-free”. e collected soil sample and ran tests on HPLC to detect traces of esticide. Results showed soil samples inevitably contained small races of pesticide residues, despite the owner seldom uses any. The ossible reason is that soil was purchased from farmers or commercial companies, who obtained soil directly from native land that is eavily polluted by pesticides due to a long history of application.

The owner was happy to try new methods to keep his precious garden “pollution & residue free”, and was little conscious of product price. He showed interest in enzyme products that degrade specific pesticide detected in his garden. He also spread the news on his Wechat and drew interested customers to us in the following weeks.

Private Orchard---Shenzhen

Family friends of Joyce own a 16 hectra private orchard in Shenzhen. Besides personal consumption, owners also grow commercialized produces, i.e. dragon fruit, lynchee, longan and papaya, primarily distribute as gift to family, friends or clients. They used heavy loads to fertilizer and pesticides, and seemed little concerned with the potential harm associated with them. However, soil quality and condition is less desirable .

They showed some interest in our products, while being price concerned. Enzyme products might be the primary choice for them, while whole cell product with automatic spraying system could be an alternative, as they consider setting up automatic watering system in the orchard soon.

Leveking Bio-tech Co.---Shenzhen

ShenZhen Leveking Biotech. Co. is a leading supplier of lipase products in Southern China. The company owns a 10,000 sqms manufacturing plant and manufactures more than 100 types of lipase products each year. Their industrial production is also strongly R&D based and mainly uses genetically engineered organisms for production.

We visited them for technique supports and industrial production advice. They provided invaluable advice on the feasibility of commercialization of our enzyme products:
1. Chassis choice---E.coli as being ‘too weak, too simple’ for large-scale production, yeast (Pichia Pastoris) or mold (Aspergillus) is a better choice. Eukaryotic cells also help with large vector (~6kb) expression problem.
2. Enzymatic activity requirement for industrial production---our current enzyme activity is low for commercialization, and other methods for testing enzyme activity were suggested
3. Additive selection for enzyme product formulation---a list of additives were provided, such as starch and dextrin
4. Inducible gene expression system---our LightOFF system is a valuable alternative to chemical induction, however its efficiency and stability needs further verification. Other cheaper chemical inducers also available.

Being a giant provider in enzyme product, Leveking also suggested a potential business collaboration opportunity with them, such as production outsourcing, which was a bonus to us. It gave us some ideas on how to reduce initial nvestment for our start-up business (refer to Business plan) and it is reflected in our business plan.

A product for the degradation of soil pesticide residues Copyright © 2017 Lucky      power by  iGEM Team:SSTi-SZGD