Difference between revisions of "Team:OUC-China/Improve"

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{{OUC-China}}
 
{{OUC-China}}
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{{OUC-China/CSS}}
 
<html>
 
<html>
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<head>
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<meta charset="utf-8">
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<title>Improve</title>
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<style type="text/css">
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body{padding-top: 50px;}
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.ouc-navbar{background-color:#008EA1;top: 15px;}
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.ouc-nav-a{color: white;}
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#ouc-logo{padding-top:5px;}
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#ouc-logo:hover{background-color:#008EA1;}
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.ouc-page-header{border-bottom-color: #EDEC8C;border-bottom-width: thick;color: #008F75;}
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.top{position: fixed;bottom:0;right: 0;}
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.top:hover{animation: upp 0.6s ;}
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@keyframes upp {
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0% {margin-bottom: 0;}
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50% {margin-bottom: 80px;}
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100% {margin-bottom: 0;}
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}
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.ouc-reserve{background-color: #008EA1;color: white;}
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.ouc-heading {color: #008EA1;}
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.ouc-a{color:#E3D434; }
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.ouc-a:hover{color: #008F75;}
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.ouc-ul{border-left:thin dashed #008F75}
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.ouc-panel-heading{text-align: center; color:#008EA1; }
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</style>
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</head>
  
  
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<body>
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<!--此处是导航-->
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<div class="navbar navbar-fixed-top ouc-navbar" role="navigation">
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    <ul class="nav navbar-nav">
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        <li><a href="https://2017.igem.org/Team:OUC-China" class="navbar-brand" id="ouc-logo"><img src="https://static.igem.org/mediawiki/2017/8/89/T--OUC-China--logo.jpg" width="66" height="40" /></a></li>
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            <a data-toggle="dropdown" class="dropdown-toggle ouc-nav-a">Team<span class="caret"></span></a>
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            <ul class="dropdown-menu">
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                <li><a href="https://2017.igem.org/Team:OUC-China/Team">Members</a></li>
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                <li><a href="#">Collaborations</a></li>
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                <li><a href="#">Attributions</a></li>
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        </li>
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        <li class="dropdown">
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            <a href="#" data-toggle="dropdown" class="dropdown-toggle ouc-nav-a">Project<spam class="caret"></span></a>
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            <ul class="dropdown-menu">
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                <li><a href="#">Description</a></li>
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                <li><a href="#">Design</a></li>
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                <li><a href="#">Experiments</a></li>
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                <li><a href="#">Results</a></li>
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                <li><a href="https://2017.igem.org/Team:OUC-China/InterLab">Interlab</a></li>
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                <li><a href="https://2017.igem.org/Team:OUC-China/Notebook" >Notebook</a></li>
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            </ul>
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        </li>
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        <li><a href="#" class="ouc-nav-a">Model</a></li>
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        <li><a href="#" class="ouc-nav-a">Parts</a></li>
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        <li><a href="#" class="ouc-nav-a">Safety</a></li>
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        <li><a href="#" class="ouc-nav-a">Human Practice</a></li>
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    </ul>
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</div>
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<!--此处是导航-->
  
  
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<div class="column full_size">
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<div class="container">
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<h1>Improve</h1>
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    <div class="page-header ouc-page-header">
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Regisrty. Please include a link to your improved part on this page.</p>
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        <h1><strong>Improve</strong></h1>
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    </div>
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<h3>Gold Medal Criterion #2</h3>
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    <h3 class="ouc-heading"><strong>Inspiration</strong></h3>
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<p><b>Standard Tracks:</b> Improve the function of an existing BioBrick Part. The original part must NOT be from your 2017 part number range. If you change the original part sequence, you must submit a new part. In addition, both the new and original part pages must reference each other. This working part must be different from the part documented in bronze #4 and silver #1.
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    <div class="row">
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    <div class="col-md-4" style="text-align: center;">
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<br><br>
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    <img src="https://static.igem.org/mediawiki/2017/f/fb/T--OUC-China--improve.5%E2%80%98UTR.png" width="400px"/>
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
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    </div>
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    <div class="col-md-8">
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    <p style="font-size: 20px">
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    <br/><br/><br/><br/>
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    5'UTR will be transcribed and can be used as a regulatory element to adjust
 +
the translation process. Thus, there is reason to believe that a delicate
 +
structure of the 5'UTR has the potential as a universal enhancement
 +
module. We see that Zhou et al. Screened some 5'UTR sequences from <i>E.
 +
coli</i>, which helped to improve protein content. Thus, we are prepared to
 +
consider this part as an enhancement module, the idea also received the
 +
author's support.
 +
    </p>
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    </div>
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    </div>
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    <h3 class="ouc-heading"><strong>Design</strong></h3>
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    <p style="text-align: center">
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    <img src="https://static.igem.org/mediawiki/2017/thumb/a/aa/T--OUC-China--improve.illustration.png/800px-T--OUC-China--improve.illustration.png" width="700px"/>
 +
    </p>
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    <p style="font-size: 20px">
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    We selected J23108 from constitutive
 +
promoter family members and added the
 +
5'UTR sequence thereafter. Fluorescent
 +
protein is used to characterize whether the
 +
5'UTR has an enhanced effect on the
 +
promoter.
 +
    </p>
 +
   
 +
    <h3 class="ouc-heading"><strong>Proof of concept</strong></h3>
 +
    <p style="text-align: center">
 +
    <img src="https://static.igem.org/mediawiki/2017/thumb/7/77/T--OUC-China--improve.FI_Abs600.png/800px-T--OUC-China--improve.FI_Abs600.png" width="700px"/>
 +
    </p>
 +
    <p style="font-size: 20px">
 +
    We constructed two red fluorescent protein devices and constructed the loop by randomly
 +
selecting the promoter and the terminator. The difference is that UTRrpsT is added to a loop and
 +
the fluorescence intensity is measured using a microplate reader and plotted. We found that the
 +
fluorescence intensity of 6h increased by 1.5 times. This indicates that UTRrpsT has the capability
 +
as a generic enhancement module.
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    </p>
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</div>
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<!--此处是置顶及赞助页-->
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<a href="#top"><img class="top" src="https://static.igem.org/mediawiki/2017/b/b7/T--OUC-China--top.jpeg" width="101" height="101" /></a>
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<div class="container-fluid ouc-reserve">
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    <p class="text-center">
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                          <br/><br/>
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                          Contact Us : oucigem@163.com&nbsp;&nbsp;|&nbsp;&nbsp;©2017 OUC IGEM.All Rights Reserved.&nbsp;&nbsp;|&nbsp;&nbsp;Based On Bootstrap<br/><br/>
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Revision as of 16:15, 29 October 2017

Improve

Improve

Inspiration





5'UTR will be transcribed and can be used as a regulatory element to adjust the translation process. Thus, there is reason to believe that a delicate structure of the 5'UTR has the potential as a universal enhancement module. We see that Zhou et al. Screened some 5'UTR sequences from E. coli, which helped to improve protein content. Thus, we are prepared to consider this part as an enhancement module, the idea also received the author's support.

Design

We selected J23108 from constitutive promoter family members and added the 5'UTR sequence thereafter. Fluorescent protein is used to characterize whether the 5'UTR has an enhanced effect on the promoter.

Proof of concept

We constructed two red fluorescent protein devices and constructed the loop by randomly selecting the promoter and the terminator. The difference is that UTRrpsT is added to a loop and the fluorescence intensity is measured using a microplate reader and plotted. We found that the fluorescence intensity of 6h increased by 1.5 times. This indicates that UTRrpsT has the capability as a generic enhancement module.



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