Line 57: | Line 57: | ||
padding: 10px; | padding: 10px; | ||
font-family: 'Karla', sans-serif; | font-family: 'Karla', sans-serif; | ||
+ | background-color: #02263e; | ||
} | } | ||
</style> | </style> |
Revision as of 18:40, 29 October 2017
RESULTS:
STEP 1: Create guideRNA Plasmid
STEP 2: Create Reporter Plasmid
STEP 3 : Create Promoter Library
STEP 4 : Random Ligations
STEP 5: Freeze drying & Revivial
STEP 6: CRISPRi & guideRNA efficiency
This picture shows our gRNAs working visually, but the graphs show the results of RFP detection using a microplate reader on cultures containing each of the dCas9 plasmids with one promoter-RFP-terminator brick, with its corresponding gRNA plasmid, or with a non-targeting gRNA plasmid.